{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T01:46:13Z","timestamp":1791942373128,"version":"4.3.4"},"reference-count":82,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2009,5,13]],"date-time":"2009-05-13T00:00:00Z","timestamp":1242172800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0005519","type":"journal-article","created":{"date-parts":[[2009,5,12]],"date-time":"2009-05-12T21:32:10Z","timestamp":1242163930000},"page":"e5519","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":118,"title":["Genome Degradation in Brucella ovis Corresponds with Narrowing of Its Host Range and Tissue Tropism"],"prefix":"10.1371","volume":"4","author":[{"given":"Renee M.","family":"Tsolis","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rekha","family":"Seshadri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renato L.","family":"Santos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felix J.","family":"Sangari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan M. Garc\u00eda","family":"Lobo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maarten F.","family":"de Jong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qinghu","family":"Ren","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Garry","family":"Myers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lauren M.","family":"Brinkac","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William C.","family":"Nelson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert T.","family":"DeBoy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samuel","family":"Angiuoli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hoda","family":"Khouri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George","family":"Dimitrov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey R.","family":"Robinson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephanie","family":"Mulligan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard L.","family":"Walker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip E.","family":"Elzer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karl A.","family":"Hassan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ian T.","family":"Paulsen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2009,5,13]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1099\/00207713-35-3-292","article-title":"Brucella, a Monospecific Genus as Shown by Deoxyribonucleic Acid Hybridization.","volume":"35","author":"J-M Verger","year":"1985","journal-title":"Int J Syst Bacteriol"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/S1369-5274(00)00165-X","article-title":"Brucellosis: a worldwide zoonosis.","volume":"4","author":"ML Boschiroli","year":"2001","journal-title":"Curr Opin Microbiol"},{"key":"ref3","first-page":"213","article-title":"Brucellosis: An Overview.","volume":"3","author":"MJ Corbel","year":"1997","journal-title":"EmergInfDis"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1073\/pnas.022622699","article-title":"Brucella melitensis: a nasty bug with hidden credentials for virulence.","volume":"99","author":"E Moreno","year":"2002","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"2688","DOI":"10.1099\/ijs.0.65269-0","article-title":"Brucella ceti sp. nov. and Brucella pinnipedialis sp. nov. for Brucella strains with cetaceans and seals as their preferred hosts.","volume":"57","author":"G Foster","year":"2007","journal-title":"Int J Syst Evol Microbiol"},{"key":"ref6","first-page":"483","article-title":"Human exposure to Brucella recovered from a sea mammal.","volume":"144","author":"SD Brew","year":"1999","journal-title":"Vet Rec"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"485","DOI":"10.3201\/eid0904.020576","article-title":"Human neurobrucellosis with intracerebral granuloma caused by a marine mammal Brucella spp.","volume":"9","author":"AH Sohn","year":"2003","journal-title":"Emerg Infect Dis"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1017\/S0022172400044612","article-title":"Studies on Brucella ovis (n.sp.), a cause of genital disease of sheep in New Zealand and Australia.","volume":"54","author":"MB Buddle","year":"1956","journal-title":"J Hyg (Lond)"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1016\/0378-1135(82)90049-9","article-title":"Ovine contagious epididymitis: a review.","volume":"7","author":"GW Burgess","year":"1982","journal-title":"Vet Microbiol"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"977","DOI":"10.2460\/javma.1987.190.08.977","article-title":"Economics of Brucella ovis control in sheep: epidemiologic simulation model.","volume":"190","author":"TE Carpenter","year":"1987","journal-title":"J Am Vet Med Assoc"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1080\/00480169.1994.35795","article-title":"Seroprevalence of Brucella ovis infection in commercial ram flocks in the Tamworth area.","volume":"42","author":"ES Sergeant","year":"1994","journal-title":"N Z Vet J"},{"key":"ref12","first-page":"75","article-title":"Brucelose ovina no Rio Grande do Sul.","volume":"16","author":"A Maghalh\u00e3es Neto","year":"1996","journal-title":"Pesquisa Veteriin\u00e1ria Brasiliera"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1023\/A:1006158514414","article-title":"Epidemiological observations in a Corriedale flock affected by Brucella ovis.","volume":"22","author":"CA Robles","year":"1998","journal-title":"Vet Res Commun"},{"key":"ref14","first-page":"241","article-title":"Presencia de anticuerpos contra diferentes especies de Brucella en sementales ovonos j\u00f3venes.","volume":"28","author":"E Torres","year":"1997","journal-title":"Veterinaria M\u00e9xico"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/S0921-4488(97)00108-9","article-title":"Diagnosis and epidemiology of Brucella ovis infection in rams.","volume":"29","author":"A Ficapal","year":"1998","journal-title":"Small Ruminant Research"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"393","DOI":"10.2460\/javma.1986.188.04.393","article-title":"Association of age of ram with distribution of epididymal lesions and etiologic agent.","volume":"188","author":"RL Walker","year":"1986","journal-title":"J Am Vet Med Assoc"},{"key":"ref17","first-page":"29","article-title":"Studies on the transmission of Brucella ovis infection in rams.","volume":"63","author":"GM Brown","year":"1973","journal-title":"Cornell Vet"},{"key":"ref18","first-page":"27","article-title":"Epididymitis in Rams. Studies on Pathogenesis.","volume":"54","author":"EL Biberstein","year":"1964","journal-title":"Cornell Vet"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1073\/pnas.221575398","article-title":"The genome sequence of the facultative intracellular pathogen Brucella melitensis.","volume":"99","author":"VG DelVecchio","year":"2002","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"12503","DOI":"10.1073\/pnas.212508599","article-title":"Comparative genome analysis of the alpha -proteobacteria: relationships between plant and animal pathogens and host specificity.","volume":"99","author":"RM Tsolis","year":"2002","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"8353","DOI":"10.1128\/IAI.73.12.8353-8361.2005","article-title":"Whole-genome analyses of speciation events in pathogenic Brucellae.","volume":"73","author":"PS Chain","year":"2005","journal-title":"Infect Immun"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"2715","DOI":"10.1128\/JB.187.8.2715-2726.2005","article-title":"Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis.","volume":"187","author":"SM Halling","year":"2005","journal-title":"J Bacteriol"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"5040","DOI":"10.1128\/JB.186.15.5040-5051.2004","article-title":"Comparative whole-genome hybridization reveals genomic islands in Brucella species.","volume":"186","author":"G Rajashekara","year":"2004","journal-title":"J Bacteriol"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"13148","DOI":"10.1073\/pnas.192319099","article-title":"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts.","volume":"99","author":"IT Paulsen","year":"2002","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"3830","DOI":"10.1128\/IAI.67.8.3830-3835.1999","article-title":"Genetic characterization of a Tn5-disrupted glycosyltransferase gene homolog in Brucella abortus and its effect on lipopolysaccharide composition and virulence.","volume":"67","author":"JR McQuiston","year":"1999","journal-title":"Infect Immun"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"821","DOI":"10.1016\/j.micinf.2004.04.009","article-title":"DNA polymorphism in the omp25\/omp31 family of Brucella spp.: identification of a 1.7-kb inversion in Brucella cetaceae and of a 15.1-kb genomic island, absent from Brucella ovis, related to the synthesis of smooth lipopolysaccharide.","volume":"6","author":"N Vizcaino","year":"2004","journal-title":"Microbes Infect"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/S0167-4781(01)00352-9","article-title":"Evidence for lateral transfer to Brucellae: characterization of a locus with a Tn-like element (Tn2020).","volume":"1574","author":"SM Halling","year":"2002","journal-title":"Biochim Biophys Acta"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"7779","DOI":"10.1128\/IAI.73.11.7779-7783.2005","article-title":"The IncP island in the genome of Brucella suis 1330 was acquired by site-specific integration.","volume":"73","author":"JP Lavigne","year":"2005","journal-title":"Infect Immun"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"3244","DOI":"10.1128\/jb.179.10.3244-3249.1997","article-title":"Genome structure and phylogeny in the genus Brucella.","volume":"179","author":"S Michaux-Charachon","year":"1997","journal-title":"J Bacteriol"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1016\/S0092-8674(02)01282-5","article-title":"Toxin-antitoxin pairs in bacteria: killers or stress regulators?","volume":"112","author":"CS Hayes","year":"2003","journal-title":"Cell"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1177\/104063879400600408","article-title":"Characteristics of a Brucella species from a bottlenose dolphin (Tursiops truncatus).","volume":"6","author":"DR Ewalt","year":"1994","journal-title":"J Vet Diagn Invest"},{"key":"ref32","first-page":"100","article-title":"Brucella-induced abortions and infection in bottlenose dolphins (Tursiops truncatus).","volume":"30","author":"WG Miller","year":"1999","journal-title":"J Zoo Wildl Med"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"3265","DOI":"10.1099\/00221287-139-12-3265","article-title":"Identification and sequence analysis of IS6501, an insertion sequence in Brucella spp.: relationship between genomic structure and the number of IS6501 copies.","volume":"139","author":"S Ouahrani","year":"1993","journal-title":"Journal of General Microbiology"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"681","DOI":"10.1111\/j.1365-2958.1994.tb01306.x","article-title":"Characterization and occurrence of two repeated palindromic DNA elements of Brucella spp.: Bru-RS1 and Bru-RS2.","volume":"14","author":"SM Halling","year":"1994","journal-title":"Mol Microbiol"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1111\/j.1462-5822.2005.00502.x","article-title":"The sheathed flagellum of Brucella melitensis is involved in persistence in a murine model of infection.","volume":"7","author":"D Fretin","year":"2005","journal-title":"Cell Microbiol"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"774","DOI":"10.1128\/IAI.01244-06","article-title":"Characterization of the urease operon of Brucella abortus and assessment of its role in virulence of the bacterium.","volume":"75","author":"FJ Sangari","year":"2007","journal-title":"Infect Immun"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1186\/1471-2180-7-57","article-title":"Brucella suis urease encoded by ure1 but not ure2 is necessary for intestinal infection of BALB\/c mice.","volume":"7","author":"AB Bandara","year":"2007","journal-title":"BMC Microbiol"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1136\/vr.144.20.555","article-title":"Experimental Brucella ovis infection in pregnant ewes.","volume":"144","author":"MJ Grillo","year":"1999","journal-title":"Vet Rec"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"998","DOI":"10.1126\/science.7754395","article-title":"The crystal structure of urease from Klebsiella aerogenes.","volume":"268","author":"E Jabri","year":"1995","journal-title":"Science"},{"key":"ref40","first-page":"1757","article-title":"Phenotypic comparison of Brucella ovis to the DNA-homologous Brucella species.","volume":"30","author":"ME Meyer","year":"1969","journal-title":"Am J Vet Res"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1099\/00221287-38-1-109","article-title":"The Metabolism of Erythritol by Brucella Abortus.","volume":"38","author":"JD Anderson","year":"1965","journal-title":"J Gen Microbiol"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1038\/193047a0","article-title":"Foetal erythritol: a cause of the localization of Brucella abortus in bovine contagious abortion.","volume":"193","author":"H Smith","year":"1962","journal-title":"Nature"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1099\/00221287-146-2-487","article-title":"The genes for erythritol catabolism are organized as an inducible operon in Brucella abortus.","volume":"146 (Pt 2)","author":"FJ Sangari","year":"2000","journal-title":"Microbiology"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"2061","DOI":"10.1099\/mic.0.28938-0","article-title":"Characterization of genes involved in erythritol catabolism in Rhizobium leguminosarum bv. viciae.","volume":"152","author":"CK Yost","year":"2006","journal-title":"Microbiology"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1128\/JB.124.1.391-397.1975","article-title":"Inhibition of growth by erythritol catabolism in Brucella abortus.","volume":"124","author":"JF Sperry","year":"1975","journal-title":"J Bacteriol"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"15711","DOI":"10.1073\/pnas.232454299","article-title":"The analysis of the intramacrophagic virulome of Brucella suis deciphers the environment encountered by the pathogen inside the macrophage host cell.","volume":"99","author":"S Kohler","year":"2002","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"1549","DOI":"10.1099\/00221287-143-5-1549","article-title":"Brucella abortus strain 2308 putative glucose and galactose transporter gene: cloning and characterization.","volume":"143 (Pt 5)","author":"RC Essenberg","year":"1997","journal-title":"Microbiology"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"4102","DOI":"10.1128\/IAI.68.7.4102-4107.2000","article-title":"Identification of genes required for chronic persistence of Brucella abortus in mice.","volume":"68","author":"PC Hong","year":"2000","journal-title":"Infect Immun"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"1973","DOI":"10.1128\/IAI.74.3.1973-1976.2006","article-title":"Requirement of norD for Brucella suis virulence in a murine model of in vitro and in vivo infection.","volume":"74","author":"S Loisel-Meyer","year":"2006","journal-title":"Infect Immun"},{"key":"ref50","doi-asserted-by":"crossref","first-page":"5332","DOI":"10.1128\/JB.182.19.5332-5341.2000","article-title":"Purine catabolism in Escherichia coli and function of xanthine dehydrogenase in purine salvage.","volume":"182","author":"H Xi","year":"2000","journal-title":"J Bacteriol"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"4911","DOI":"10.1128\/IAI.72.8.4911-4917.2004","article-title":"Intact purine biosynthesis pathways are required for wild-type virulence of Brucella abortus 2308 in the BALB\/c mouse model.","volume":"72","author":"RB Alcantara","year":"2004","journal-title":"Infect Immun"},{"key":"ref52","doi-asserted-by":"crossref","first-page":"4031","DOI":"10.1128\/IAI.72.7.4031-4039.2004","article-title":"Oral vaccination with Brucella melitensis WR201 protects mice against intranasal challenge with virulent Brucella melitensis 16M.","volume":"72","author":"MJ Izadjoo","year":"2004","journal-title":"Infect Immun"},{"key":"ref53","doi-asserted-by":"crossref","first-page":"929","DOI":"10.1128\/jb.171.2.929-939.1989","article-title":"Rhizobium meliloti fixGHI sequence predicts involvement of a specific cation pump in symbiotic nitrogen fixation.","volume":"171","author":"D Kahn","year":"1989","journal-title":"J Bacteriol"},{"key":"ref54","doi-asserted-by":"crossref","first-page":"1008","DOI":"10.1128\/IAI.66.3.1008-1016.1998","article-title":"Transposon-derived Brucella abortus rough mutants are attenuated and exhibit reduced intracellular survival.","volume":"66","author":"CA Allen","year":"1998","journal-title":"Infect Immun"},{"key":"ref55","doi-asserted-by":"crossref","first-page":"5485","DOI":"10.1128\/IAI.66.11.5485-5493.1998","article-title":"Identification of the perosamine synthetase gene of Brucella melitensis 16M and involvement of lipopolysaccharide O side chain in Brucella survival in mice and in macrophages.","volume":"66","author":"F Godfroid","year":"1998","journal-title":"Infect Immun"},{"key":"ref56","doi-asserted-by":"crossref","first-page":"6181","DOI":"10.1128\/IAI.67.11.6181-6186.1999","article-title":"The outer membrane of Brucella ovis shows increased permeability to hydrophobic probes and is more susceptible to cationic peptides than are the outer membranes of mutant rough Brucella abortus strains.","volume":"67","author":"E Freer","year":"1999","journal-title":"Infect Immun"},{"key":"ref57","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1111\/j.1462-5822.2005.00629.x","article-title":"Differential inductions of TNF-alpha and IGTP, IIGP by structurally diverse classic and non-classic lipopolysaccharides.","volume":"8","author":"N Lapaque","year":"2006","journal-title":"Cell Microbiol"},{"key":"ref58","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1046\/j.1462-5822.2001.00131.x","article-title":"Identification of Brucella spp. genes involved in intracellular trafficking.","volume":"3","author":"RM Delrue","year":"2001","journal-title":"Cell Microbiol"},{"key":"ref59","doi-asserted-by":"crossref","first-page":"1210","DOI":"10.1046\/j.1365-2958.1999.01569.x","article-title":"A homologue of the Agrobacterium tumefaciens VirB and Bordetella pertussis Ptl type IV secretion systems is essential for intracellular survival of Brucella suis.","volume":"33","author":"D O'Callaghan","year":"1999","journal-title":"Mol Microbiol"},{"key":"ref60","doi-asserted-by":"crossref","first-page":"4849","DOI":"10.1128\/JB.182.17.4849-4855.2000","article-title":"A homologue of an operon required for DNA transfer in Agrobacterium is required in Brucella abortus for virulence and intracellular multiplication.","volume":"182","author":"R Sieira","year":"2000","journal-title":"J Bacteriol"},{"key":"ref61","doi-asserted-by":"crossref","first-page":"1151","DOI":"10.1111\/j.1462-5822.2005.00543.x","article-title":"A quorum-sensing regulator controls expression of both the type IV secretion system and the flagellar apparatus of Brucella melitensis.","volume":"7","author":"RM Delrue","year":"2005","journal-title":"Cell Microbiol"},{"key":"ref62","doi-asserted-by":"crossref","first-page":"1075","DOI":"10.1128\/IAI.71.3.1075-1082.2003","article-title":"Production of the type IV secretion system differs among Brucella species as revealed with VirB5- and VirB8-specific antisera.","volume":"71","author":"B Rouot","year":"2003","journal-title":"Infect Immun"},{"key":"ref63","article-title":"Identification of VceA and VceC, two members of the VjbR regulon that are translocated into macrophages by the Brucella type IV secretion system.","author":"MF de Jong","year":"2008","journal-title":"Mol Microbiol"},{"key":"ref64","doi-asserted-by":"crossref","first-page":"706","DOI":"10.1016\/j.micinf.2008.02.013","article-title":"Importance of the Omp25\/Omp31 family in the internalization and intracellular replication of virulent B. ovis in murine macrophages and HeLa cells.","volume":"10","author":"AI Martin-Martin","year":"2008","journal-title":"Microbes Infect"},{"key":"ref65","doi-asserted-by":"crossref","first-page":"e2193","DOI":"10.1371\/journal.pone.0002193","article-title":"Genome sequence of Brucella abortus vaccine strain S19 compared to virulent strains yields candidate virulence genes.","volume":"3","author":"OR Crasta","year":"2008","journal-title":"PLoS ONE"},{"key":"ref66","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/j.vetmic.2005.05.012","article-title":"Putative outer membrane autotransporter protein influences survival of Brucella suis in BALB\/c mice.","volume":"109","author":"AB Bandara","year":"2005","journal-title":"Vet Microbiol"},{"key":"ref67","doi-asserted-by":"crossref","first-page":"618","DOI":"10.1038\/ni1202","article-title":"Cyclic beta-1,2-glucan is a Brucella virulence factor required for intracellular survival.","volume":"6","author":"B Arellano-Reynoso","year":"2005","journal-title":"Nat Immunol"},{"key":"ref68","doi-asserted-by":"crossref","first-page":"4528","DOI":"10.1128\/IAI.69.7.4528-4535.2001","article-title":"Brucella abortus cyclic beta-1,2-glucan mutants have reduced virulence in mice and are defective in intracellular replication in HeLa cells.","volume":"69","author":"G Briones","year":"2001","journal-title":"Infect Immun"},{"key":"ref69","doi-asserted-by":"crossref","first-page":"e21","DOI":"10.1371\/journal.ppat.0040021","article-title":"Brucella control of dendritic cell maturation is dependent on the TIR-containing protein Btp1.","volume":"4","author":"SP Salcedo","year":"2008","journal-title":"PLoS Pathog"},{"key":"ref70","doi-asserted-by":"crossref","first-page":"16514","DOI":"10.1073\/pnas.0603362103","article-title":"A B lymphocyte mitogen is a Brucella abortus virulence factor required for persistent infection.","volume":"103","author":"JM Spera","year":"2006","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref71","doi-asserted-by":"crossref","first-page":"12375","DOI":"10.1073\/pnas.192439399","article-title":"The two-component system BvrR\/BvrS essential for Brucella abortus virulence regulates the expression of outer membrane proteins with counterparts in members of the Rhizobiaceae.","volume":"99","author":"C Guzman-Verri","year":"2002","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref72","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1046\/j.1365-2958.1998.00913.x","article-title":"A two-component regulatory system playing a critical role in plant pathogens and endosymbionts is present in Brucella abortus and controls cell invasion and virulence.","volume":"29","author":"A Sola-Landa","year":"1998","journal-title":"Mol Microbiol"},{"key":"ref73","doi-asserted-by":"crossref","first-page":"3785","DOI":"10.1128\/IAI.61.9.3785-3790.1993","article-title":"The omp2 gene locus of Brucella abortus encodes two homologous outer membrane proteins with properties characteristic of bacterial porins.","volume":"61","author":"H Marquis","year":"1993","journal-title":"Infect Immun"},{"key":"ref74","doi-asserted-by":"crossref","first-page":"3744","DOI":"10.1128\/IAI.64.9.3744-3751.1996","article-title":"Cloning, nucleotide sequence, and expression of the Brucella melitensis omp31 gene coding for an immunogenic major outer membrane protein.","volume":"64","author":"N Vizcaino","year":"1996","journal-title":"Infect Immun"},{"key":"ref75","first-page":"351","article-title":"Brucella ovis.","author":"JM Blasco","year":"1990"},{"key":"ref76","first-page":"145","article-title":"Urease activity may contribute to the ability of Actinobacillus pleuropneumoniae to establish infection.","volume":"64","author":"JT Bosse","year":"2000","journal-title":"Can J Vet Res"},{"key":"ref77","doi-asserted-by":"crossref","DOI":"10.1007\/978-3-642-59533-2_14","article-title":"Genitourinary Brucellosis.","author":"MM Madkour","year":"2001"},{"key":"ref78","doi-asserted-by":"crossref","first-page":"580","DOI":"10.1038\/37551","article-title":"Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi.","volume":"390","author":"CM Fraser","year":"1997","journal-title":"Nature"},{"key":"ref79","doi-asserted-by":"crossref","first-page":"500","DOI":"10.1006\/geno.1999.6048","article-title":"Optimized multiplex PCR: efficiently closing a whole-genome shotgun sequencing project.","volume":"62","author":"H Tettelin","year":"1999","journal-title":"Genomics"},{"key":"ref80","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1093\/bioinformatics\/btm009","article-title":"Identifying bacterial genes and endosymbiont DNA with Glimmer.","volume":"23","author":"AL Delcher","year":"2007","journal-title":"Bioinformatics"},{"key":"ref81","doi-asserted-by":"crossref","first-page":"2478","DOI":"10.1093\/nar\/30.11.2478","article-title":"Fast algorithms for large-scale genome alignment and comparison.","volume":"30","author":"AL Delcher","year":"2002","journal-title":"Nucleic Acids Res"},{"key":"ref82","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1038\/nbt1302","article-title":"Genome sequence and identification of candidate vaccine antigens from the animal pathogen Dichelobacter nodosus.","volume":"25","author":"GS Myers","year":"2007","journal-title":"Nat Biotechnol"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0005519","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,9]],"date-time":"2025-02-09T11:06:05Z","timestamp":1739099165000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0005519"}},"subtitle":[],"editor":[{"given":"Niyaz","family":"Ahmed","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2009,5,13]]},"references-count":82,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2009,5,13]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0005519","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,5,13]]}}}