{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T17:56:03Z","timestamp":1791741363960,"version":"4.3.4"},"reference-count":38,"publisher":"Public Library of Science (PLoS)","issue":"8","license":[{"start":{"date-parts":[[2009,8,5]],"date-time":"2009-08-05T00:00:00Z","timestamp":1249430400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0006496","type":"journal-article","created":{"date-parts":[[2009,8,4]],"date-time":"2009-08-04T22:21:07Z","timestamp":1249424467000},"page":"e6496","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":74,"title":["Immunogenic Burkholderia pseudomallei Outer Membrane Proteins as Potential Candidate Vaccine Targets"],"prefix":"10.1371","volume":"4","author":[{"given":"Yuka","family":"Hara","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rahmah","family":"Mohamed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheila","family":"Nathan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2009,8,5]]},"reference":[{"key":"ref1","first-page":"72","article-title":"Melioidosis.","volume":"96","author":"DA Dance","year":"2002","journal-title":"Curr Opin Infect"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1097\/00001432-200404000-00011","article-title":"Recent development in melioidosis.","volume":"17","author":"A Leelarasamee","year":"2004","journal-title":"Curr Opin Infect Dis"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1128\/CMR.18.2.383-416.2005","article-title":"Melioidosis: Epidemiology, pathophysiology and management.","volume":"18","author":"AC Cheng","year":"2005","journal-title":"Clin Microbiol Rev"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"1241","DOI":"10.1086\/508217","article-title":"A live experimental vaccine against <italic>Burkholderia pseudomallei<\/italic> elicits CD4+ T cell-mediated immunity, priming T cells specific for 2 type III__ secretion system proteins.","volume":"194","author":"A Haque","year":"2006","journal-title":"J Infect Dis"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"927","DOI":"10.1086\/517253","article-title":"Arabinose-positive <italic>Burkholderia pseudomallei<\/italic> infection in humans: case report.","volume":"28","author":"N Lertpatanasuwan","year":"1999","journal-title":"Clin Infect Dis"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"5945","DOI":"10.1128\/IAI.73.9.5945-5951.2005","article-title":"Humoral and cell-mediated adaptive immune responses are required for protection against <italic>Burkholderia pseudomallei<\/italic> challenge and bacterial clearance post-infection.","volume":"73","author":"GD Healey","year":"2005","journal-title":"Infect Immun"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"2824","DOI":"10.1128\/IAI.64.7.2824-2828.1996","article-title":"Structural and immunological characterization of <italic>Burkholderia pseudomallei<\/italic> O-polysaccharide-flagellin protein conjugates.","volume":"64","author":"PJ Brett","year":"1996","journal-title":"Infect Immun"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"1177","DOI":"10.1099\/jmm.0.45766-0","article-title":"Evaluation of lipopolysaccharide and capsular polysaccharide as subunit vaccines against experimental melioidosis.","volume":"53","author":"M Nelson","year":"2004","journal-title":"J Med Microbiol"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"750","DOI":"10.1016\/j.vaccine.2005.08.069","article-title":"Immunogenicity and anti-<italic>Burkholderia pseudomallei<\/italic> activity in BALB\/c mice immunized with plasmid DNA encoding flagellin.","volume":"24","author":"YS Chen","year":"2005","journal-title":"Vaccine"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1111\/j.1574-695X.1998.tb01219.x","article-title":"Antisera to selected outer membrane proteins of <italic>Vibrio cholerae<\/italic> protect against challenge with homologous and heterologous strains of <italic>V. cholerae<\/italic>.","volume":"22","author":"M Das","year":"1998","journal-title":"FEMS Immunol Med Microbiol"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"2252","DOI":"10.1128\/IAI.59.7.2252-2258.1991","article-title":"Outer membrane protein A (OmpA) contributes to serum resistance and pathogenicity of <italic>Escherichia coli<\/italic> K-1.","volume":"59","author":"JN Weiser","year":"1991","journal-title":"Infect Immun"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1046\/j.0902-0055.2003.00096.x","article-title":"Characterization of two outer membrane protein antigens of <italic>Porphyromonas gingivalis<\/italic> that are protective in a murine lesion model.","volume":"19","author":"BC Ross","year":"2004","journal-title":"Oral Microbiol Immunol"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1016\/S1286-4579(01)01417-4","article-title":"<italic>Burkholdeira pseudomallei<\/italic> virulence: definition, stability and association with clonality.","volume":"3","author":"GC Ulett","year":"2001","journal-title":"Microbes Infect"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"1263","DOI":"10.1016\/j.micinf.2005.04.013","article-title":"A model of immunity for <italic>Burkholderia pseudomallei<\/italic> unique responses following immunization and acute lethal infection.","volume":"7","author":"GC Ulett","year":"2005","journal-title":"Microbes Infect"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1111\/j.1365-2958.2004.04528.x","article-title":"Identification of a bacterial factor required for actin-based motility of <italic>Burkholderia pseudomallei<\/italic>.","volume":"56","author":"MP Stevens","year":"2005","journal-title":"Mol Microbiol"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1128\/CDLI.7.2.168-174.2000","article-title":"Characterization of a predominant immunogenic outer membrane protein of <italic>Riemerella anatipestifer<\/italic>.","volume":"7","author":"S Subramaniam","year":"2000","journal-title":"Clin Diagn Lab Immunol"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"1027","DOI":"10.1111\/j.1365-2958.2003.03903.x","article-title":"Structure of the OmpA-like domain of RmpM from <italic>Neisseria meningitidis<\/italic>.","volume":"51","author":"S Grizot","year":"2004","journal-title":"Mol Microbiol"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"7285","DOI":"10.4049\/jimmunol.165.12.7285","article-title":"Direct detection and magnetic isolation of <italic>Chlamydia trachomatis<\/italic> major outer membrane protein-specific CD8+ CTLs with HLA class I tetramers.","volume":"165","author":"SK Kim","year":"2000","journal-title":"J Immunol"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"A23","DOI":"10.1016\/S0264-410X(02)00383-3","article-title":"Outer membrane protein A (OmpA): a new pathogen associated molecular pattern that interacts with antigen presenting cells-impact on vaccine strategies.","volume":"20","author":"P Jeannin","year":"2002","journal-title":"Vaccine"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1007\/978-1-4899-1382-1_11","article-title":"Bacterial outer membrane protein vaccines: The meningococcal example.","volume":"397","author":"JT Poolman","year":"1996","journal-title":"Adv Exp Med"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1006\/bbrc.2002.6657","article-title":"The function of OmpA in <italic>Escherichia coli<\/italic>.","volume":"292","author":"Y Wang","year":"2002","journal-title":"Biochem and Biophys Res Commun"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"1691","DOI":"10.1128\/IAI.58.6.1691-1696.1990","article-title":"A strong antibody response to the periplasmic C-terminal domain of the OmpA protein of <italic>Escherichia coli<\/italic> is produced by immunization with purified OmpA or with whole <italic>E. coli<\/italic> or <italic>Salmonella typhimurium<\/italic> bacteria.","volume":"58","author":"R Puohiniemi","year":"1990","journal-title":"Infect Immun"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"3593","DOI":"10.1128\/IAI.67.7.3593-3600.1999","article-title":"Obligatory role of gamma interferon for host survival in a murine model of infection with <italic>Burkholderia pseudomallei<\/italic>.","volume":"67","author":"P Santanirand","year":"1999","journal-title":"Infect Immun"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"944","DOI":"10.1126\/science.3107127","article-title":"Interferon-gamma and B cell stimulatory factor-1 reciprocally regulate Ig isotype production.","volume":"236","author":"CM Snapper","year":"1987","journal-title":"Science"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"1155","DOI":"10.1128\/IAI.71.3.1155-1160.2003","article-title":"Expression and immunogenicity of <italic>Mycoplasma hyopneumoniae<\/italic> heat shock protein antigen P42 by DNA vaccination.","volume":"71","author":"YL Chen","year":"2003","journal-title":"Infect Immun"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"782","DOI":"10.1128\/IAI.64.3.782-790.1996","article-title":"Intracellular survival of <italic>Burkholderia pseudomallei<\/italic>.","volume":"64","author":"L Jones","year":"1996","journal-title":"Infect Immun"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"1181","DOI":"10.1093\/infdis\/168.5.1181","article-title":"Relapse in melioidosis: incidence and risk factors.","volume":"168","author":"W Chaowagul","year":"1993","journal-title":"J Infect Dis"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"1706","DOI":"10.1128\/IAI.74.3.1706-1711.2006","article-title":"Protection against heterologous <italic>Burkholderia pseudomallei<\/italic> strains by dendritic cell immunization.","volume":"74","author":"SJ Elvin","year":"2006","journal-title":"Infect Immun"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/S0022-2836(05)80360-2","article-title":"Basic local alignment search tool.","volume":"215","author":"SF Altschul","year":"1990","journal-title":"J Mol Biol"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"944","DOI":"10.1093\/bioinformatics\/16.10.944","article-title":"Artemis: sequence visualisation and annotation.","volume":"16","author":"K Rutherford","year":"2000","journal-title":"Bioinformatics"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"2947","DOI":"10.1093\/bioinformatics\/btm404","article-title":"ClustalW and ClustalX version 2.","volume":"23","author":"MA Larkin","year":"2007","journal-title":"Bioinformatics"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1093\/bioinformatics\/btg430","article-title":"The Jalview Java alignment editor.","volume":"20","author":"M Clamp","year":"2004","journal-title":"Bioinformatics"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/j.diagmicrobio.2007.01.002","article-title":"<italic>Burkholderia pseudomallei<\/italic> animal and human isolates from Malaysia exhibit different phenotypic characteristics.","volume":"58","author":"SH Lee","year":"2007","journal-title":"Diag Microbiol Infect Dis"},{"key":"ref34","article-title":"Molecular Cloning: a Laboratory Manual, 3rd edn.","author":"J Sambrook","year":"2001"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/0003-2697(85)90442-7","article-title":"Measurement of protein using bicinchoninic acid.","volume":"150","author":"PK Smith","year":"1985","journal-title":"Anal Biochem"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of bacteriophage T4.","volume":"227","author":"UK Laemmli","year":"1970","journal-title":"Nature"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"4350","DOI":"10.1073\/pnas.76.9.4350","article-title":"Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.","volume":"76","author":"H Towbin","year":"1979","journal-title":"Proc Natl Acad Sci USA"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"370","DOI":"10.1016\/j.enzmictec.2006.09.014","article-title":"The <italic>Burkholderia pseudomallei<\/italic> serine protease MprA is auto-proteolytically activated to produce a highly stable enzyme.","volume":"40","author":"CY Chin","year":"2007","journal-title":"Enzyme Microbiol Technol"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0006496","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,21]],"date-time":"2020-05-21T05:25:50Z","timestamp":1590038750000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0006496"}},"subtitle":[],"editor":[{"given":"Ramy K.","family":"Aziz","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2009,8,5]]},"references-count":38,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2009,8,5]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0006496","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,8,5]]}}}