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Bopp","year":"1991","journal-title":"Genes Dev"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1093\/genetics\/139.2.631","article-title":"Transposon insertions causing constitutive Sex-lethal activity in <italic>Drosophila melanogaster<\/italic> affect <italic>Sxl<\/italic> sex-specific transcript splicing.","volume":"139","author":"M Bernstein","year":"1995","journal-title":"Genetics"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"22227","DOI":"10.1074\/jbc.272.35.22227","article-title":"Sex-lethal interactions with protein and RNA. 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