{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T15:31:46Z","timestamp":1759332706857},"reference-count":32,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2009,9,4]],"date-time":"2009-09-04T00:00:00Z","timestamp":1252022400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0006905","type":"journal-article","created":{"date-parts":[[2009,9,3]],"date-time":"2009-09-03T21:50:44Z","timestamp":1252014644000},"page":"e6905","update-policy":"http:\/\/dx.doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":17,"title":["DR*W201\/P65 Tetramer Visualization of Epitope-Specific CD4 T-Cell during M. tuberculosis Infection and Its Resting Memory Pool after BCG Vaccination"],"prefix":"10.1371","volume":"4","author":[{"given":"Huiyong","family":"Wei","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhuqing","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Crystal Y.","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dan","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lisa","family":"Halliday","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weihua","family":"Zhong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gucheng","family":"Zeng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yun","family":"Shen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ling","family":"Shen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunqi","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zheng W.","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2009,9,4]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"1557","DOI":"10.1182\/blood-2008-05-078154","article-title":"CD4 T cells: fates, functions, and faults.","volume":"112","author":"J Zhu","year":"2008","journal-title":"Blood"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"1962","DOI":"10.4049\/jimmunol.180.3.1962","article-title":"Distinct, specific IL-17- and IL-22-producing CD4+ T cell subsets contribute to the human anti-mycobacterial immune response.","volume":"180","author":"TJ Scriba","year":"2008","journal-title":"J Immunol"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1086\/524685","article-title":"Mycobacterial T cell responses in HIV-infected patients with advanced immunosuppression.","volume":"197","author":"AS Hammond","year":"2008","journal-title":"J Infect Dis"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/j.autrev.2008.07.011","article-title":"Screening for tuberculosis infection prior to initiation of anti-TNF therapy.","volume":"8","author":"A Lalvani","year":"2008","journal-title":"Autoimmun Rev"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"1167","DOI":"10.1172\/JCI38482","article-title":"Anti-TNF immunotherapy reduces CD8+ T cell-mediated antimicrobial activity against Mycobacterium tuberculosis in humans.","volume":"119","author":"H Bruns","year":"2009","journal-title":"J Clin Invest"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1073\/pnas.97.1.291","article-title":"Use of soluble peptide-DR4 tetramers to detect synovial T cells specific for cartilage antigens in patients with rheumatoid arthritis.","volume":"97","author":"BL Kotzin","year":"2000","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1016\/S1074-7613(00)80572-5","article-title":"Detection of antigen-specific T cells with multivalent soluble class II MHC covalent peptide complexes.","volume":"8","author":"F Crawford","year":"1998","journal-title":"Immunity"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1126\/science.274.5284.94","article-title":"Phenotypic analysis of antigen-specific T lymphocytes.","volume":"274","author":"JD Altman","year":"1996","journal-title":"Science"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"R63","DOI":"10.1172\/JCI8476","article-title":"MHC class II tetramers identify peptide-specific human CD4(+) T cells proliferating in response to influenza A antigen.","volume":"104","author":"EJ Novak","year":"1999","journal-title":"J Clin Invest"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1111\/j.1365-2567.2007.02801.x","article-title":"Class II major histocompatibility complex tetramer staining: progress, problems, and prospects.","volume":"123","author":"SS Vollers","year":"2008","journal-title":"Immunology"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1007\/978-1-4615-5355-7_14","article-title":"In vivo dynamics of anti-viral CD8 T cell responses to different epitopes. An evaluation of bystander activation in primary and secondary responses to viral infection.","volume":"452","author":"K Murali-Krishna","year":"1998","journal-title":"Adv Exp Med Biol"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"1765","DOI":"10.4049\/jimmunol.162.3.1765","article-title":"Direct analysis of viral-specific CD8+ T cells with soluble HLA-A2\/Tax11-19 tetramer complexes in patients with human T cell lymphotropic virus-associated myelopathy.","volume":"162","author":"K Bieganowska","year":"1999","journal-title":"J Immunol"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"1827","DOI":"10.4049\/jimmunol.162.3.1827","article-title":"A re-evaluation of the frequency of CD8+ T cells specific for EBV in healthy virus carriers.","volume":"162","author":"LC Tan","year":"1999","journal-title":"J Immunol"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"950","DOI":"10.1016\/j.humimm.2005.06.011","article-title":"Direct ex vivo detection of HLA-DR3-restricted cytomegalovirus- and Mycobacterium tuberculosis-specific CD4+ T cells.","volume":"66","author":"C Bronke","year":"2005","journal-title":"Hum Immunol"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"8751","DOI":"10.1128\/JVI.74.18.8751-8756.2000","article-title":"Human immunodeficiency virus type 1 envelope epitope-specific CD4(+) T lymphocytes in simian\/human immunodeficiency virus-infected and vaccinated rhesus monkeys detected using a peptide-major histocompatibility complex class II tetramer.","volume":"74","author":"MJ Kuroda","year":"2000","journal-title":"J Virol"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"4244","DOI":"10.4049\/jimmunol.164.8.4244","article-title":"HLA-DQ tetramers identify epitope-specific T cells in peripheral blood of herpes simplex virus type 2-infected individuals: direct detection of immunodominant antigen-responsive cells.","volume":"164","author":"WW Kwok","year":"2000","journal-title":"J Immunol"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"11433","DOI":"10.1073\/pnas.190335897","article-title":"Direct enumeration of Borrelia-reactive CD4 T cells ex vivo by using MHC class II tetramers.","volume":"97","author":"AL Meyer","year":"2000","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"2174","DOI":"10.4049\/jimmunol.174.4.2174","article-title":"Highly focused T cell responses in latent human pulmonary Mycobacterium tuberculosis infection.","volume":"174","author":"G Tully","year":"2005","journal-title":"J Immunol"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"14023","DOI":"10.1128\/JVI.78.24.14023-14032.2004","article-title":"Clinical latency and reactivation of AIDS-related mycobacterial infections.","volume":"78","author":"Y Shen","year":"2004","journal-title":"J Virol"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"6152","DOI":"10.4049\/jimmunol.172.10.6152","article-title":"Genetic makeup of the DR region in rhesus macaques: gene content, transcripts, and pseudogenes.","volume":"172","author":"N de Groot","year":"2004","journal-title":"J Immunol"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1128\/IAI.01008-07","article-title":"Immune distribution and localization of phosphoantigen-specific Vgamma2Vdelta2 T cells in lymphoid and nonlymphoid tissues in Mycobacterium tuberculosis infection.","volume":"76","author":"D Huang","year":"2008","journal-title":"Infect Immun"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"4798","DOI":"10.4049\/jimmunol.181.7.4798","article-title":"Definition of APC presentation of phosphoantigen (E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate to Vgamma2Vdelta2 TCR.","volume":"181","author":"H Wei","year":"2008","journal-title":"J Immunol"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"831","DOI":"10.1084\/jem.171.3.831","article-title":"Recognition of a mycobacteria-specific epitope in the 65-kD heat-shock protein by synovial fluid-derived T cell clones.","volume":"171","author":"JS Gaston","year":"1990","journal-title":"J Exp Med"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"8287","DOI":"10.4049\/jimmunol.179.12.8287","article-title":"Prolonged (E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate-driven antimicrobial and cytotoxic responses of pulmonary and systemic Vgamma2Vdelta2 T cells in macaques.","volume":"179","author":"Z Ali","year":"2007","journal-title":"J Immunol"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/j.immuni.2007.07.007","article-title":"Naive CD4(+) T cell frequency varies for different epitopes and predicts repertoire diversity and response magnitude.","volume":"27","author":"JJ Moon","year":"2007","journal-title":"Immunity"},{"key":"ref26","article-title":"Phosphoantigen-activated V{gamma}2V{delta}2 T cells antagonize IL-2-induced CD4+CD25+Foxp3+ T regulatory cells.","author":"G Gong","year":"2008","journal-title":"Blood"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"4525","DOI":"10.4049\/jimmunol.166.7.4525","article-title":"The TCR repertoire of an immunodominant CD8+ T lymphocyte population.","volume":"166","author":"ZW Chen","year":"2001","journal-title":"J Immunol"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1111\/j.1365-3083.2007.01924.x","article-title":"MHC class II tetramer guided detection of Mycobacterium tuberculosis-specific CD4+ T cells in peripheral blood from patients with pulmonary tuberculosis.","volume":"65","author":"H Hohn","year":"2007","journal-title":"Scand J Immunol"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1128\/IAI.71.1.483-493.2003","article-title":"Mapping of murine Th1 helper T-Cell epitopes of mycolyl transferases Ag85A, Ag85B, and Ag85C from Mycobacterium tuberculosis.","volume":"71","author":"S D'Souza","year":"2003","journal-title":"Infect Immun"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"6334","DOI":"10.4049\/jimmunol.175.10.6334","article-title":"Ultrasensitive detection and phenotyping of CD4+ T cells with optimized HLA class II tetramer staining.","volume":"175","author":"TJ Scriba","year":"2005","journal-title":"J Immunol"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"10958","DOI":"10.1128\/JVI.75.22.10958-10968.2001","article-title":"Molecular determinants of peptide binding to two common rhesus macaque major histocompatibility complex class II molecules.","volume":"75","author":"JL Dzuris","year":"2001","journal-title":"J Virol"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"2740","DOI":"10.1016\/j.vaccine.2004.11.051","article-title":"Protection of macaques against Mycobacterium tuberculosis infection by a subunit vaccine based on a fusion protein of antigen 85B and ESAT-6.","volume":"23","author":"JA Langermans","year":"2005","journal-title":"Vaccine"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0006905","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,26]],"date-time":"2023-05-26T15:58:13Z","timestamp":1685116693000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0006905"}},"subtitle":[],"editor":[{"given":"Madhukar","family":"Pai","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2009,9,4]]},"references-count":32,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2009,9,4]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0006905","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,9,4]]}}}