{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T08:05:59Z","timestamp":1790409959834,"version":"4.1.0"},"reference-count":43,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2009,9,16]],"date-time":"2009-09-16T00:00:00Z","timestamp":1253059200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0007041","type":"journal-article","created":{"date-parts":[[2009,9,15]],"date-time":"2009-09-15T23:54:20Z","timestamp":1253058860000},"page":"e7041","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":36,"title":["Complete Genome Sequence of Francisella tularensis Subspecies holarctica FTNF002-00"],"prefix":"10.1371","volume":"4","author":[{"given":"Ravi D.","family":"Barabote","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gary","family":"Xie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas S.","family":"Brettin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven H.","family":"Hinrichs","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul D.","family":"Fey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Justin J.","family":"Jay","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer L.","family":"Engle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shubhada D.","family":"Godbole","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jyothi M.","family":"Noronha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard H.","family":"Scheuermann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liwei W.","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Lion","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael P.","family":"Dempsey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2009,9,16]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1093\/infdis\/10.1.61","article-title":"Further observations on a plague-like disease of rodents with a preliminary note on the causative agent, <italic>Bacterium tularense<\/italic>.","volume":"10","author":"GW McCoy","year":"1912","journal-title":"J Infect Dis"},{"key":"ref2","first-page":"113","article-title":"Tularemia;","author":"CE Hopla","year":"1994"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"5808","DOI":"10.1128\/JB.186.17.5808-5818.2004","article-title":"Worldwide genetic relationships among <italic>Francisella tularensis<\/italic> isolates determined by multiple-locus variable-number tandem repeat analysis.","volume":"186","author":"A Johansson","year":"2004","journal-title":"J Bacteriol"},{"key":"ref4","article-title":"Chapter 24: Tularemia.","author":"ME Evans","year":"1997"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1196\/annals.1409.011","article-title":"Molecular epidemiology, evolution, and ecology of <italic>Francisella<\/italic>.","volume":"1105","author":"P Keim","year":"2007","journal-title":"Ann N Y Acad Sci"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1128\/CMR.15.4.631-646.2002","article-title":"Tularemia.","volume":"15","author":"J Ellis","year":"2002","journal-title":"Clin Microbiol Rev"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"3903","DOI":"10.1128\/JB.187.11.3903-3908.2005","article-title":"Evolution of subspecies of <italic>Francisella tularensis<\/italic>.","volume":"187","author":"K Svensson","year":"2005","journal-title":"J Bacteriol"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"1113","DOI":"10.3201\/eid1207.051504","article-title":"Epidemiologic and molecular analysis of human tularemia, United States, 1964-2004.","volume":"12","author":"JE Staples","year":"2006","journal-title":"Emerg Infect Dis"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"2924","DOI":"10.1128\/JCM.41.7.2924-2931.2003","article-title":"Genome-wide DNA microarray analysis of <italic>Francisella tularensis<\/italic> strains demonstrates extensive genetic conservation within the species but identifies regions that are unique to the highly virulent <italic>F. tularensis<\/italic> subsp. <italic>tularensis<\/italic>.","volume":"41","author":"M Broekhuijsen","year":"2003","journal-title":"J Clin Microbiol"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1111\/j.1574-6968.2004.tb09672.x","article-title":"Genome diversity among regional populations of <italic>Francisella tularensis<\/italic> subspecies <italic>tularensis<\/italic> and <italic>Francisella tularensis<\/italic> subspecies <italic>holarctica<\/italic> isolated from the US.","volume":"237","author":"MM Samrakandi","year":"2004","journal-title":"FEMS Microbiol Lett"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"6430","DOI":"10.1128\/JB.186.19.6430-6436.2004","article-title":"A <italic>Francisella tularensis<\/italic> pathogenicity island required for intramacrophage growth.","volume":"186","author":"FE Nano","year":"2004","journal-title":"J Bacteriol"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"5904","DOI":"10.1128\/JB.00437-06","article-title":"Paired-end sequence mapping detects extensive genomic rearrangement and translocation during divergence of <italic>Francisella tularensis<\/italic> subsp. <italic>tularensis<\/italic> and <italic>Francisella tularensis<\/italic> subsp. <italic>holarctica<\/italic> populations.","volume":"188","author":"MP Dempsey","year":"2006","journal-title":"J Bacteriol"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"6977","DOI":"10.1128\/JB.00506-06","article-title":"Chromosome rearrangement and diversification of <italic>Francisella tularensis<\/italic> revealed by the type B (OSU18) genome sequence.","volume":"188","author":"JF Petrosino","year":"2006","journal-title":"J Bacteriol"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1051\/vetres:2005006","article-title":"Tularemia: emergence\/re-emergence.","volume":"36","author":"JM Petersen","year":"2005","journal-title":"Vet Res"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"1835","DOI":"10.3201\/eid1112.050728","article-title":"<italic>Francisella tularensis<\/italic> in the United States.","volume":"11","author":"J Farlow","year":"2005","journal-title":"Emerg Infect Dis"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"7465","DOI":"10.1128\/AEM.00646-07","article-title":"Genomic deletion marking an emerging subclone of <italic>Francisella tularensis<\/italic> subsp. <italic>holarctica<\/italic> in France and the Iberian Peninsula.","volume":"73","author":"MP Dempsey","year":"2007","journal-title":"Appl Environ Microbiol"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"2474","DOI":"10.1128\/JB.01786-08","article-title":"Phylogeography of <italic>Francisella tularensis<\/italic>: global expansion of a highly fit clone.","volume":"191","author":"AJ Vogler","year":"2009","journal-title":"J Bacteriol"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"1016","DOI":"10.1128\/JCM.38.3.1016-1022.2000","article-title":"Comparison of different PCR approaches for typing of <italic>Francisella tularensis<\/italic> strains.","volume":"38","author":"VA de la Puente-Redondo","year":"2000","journal-title":"J Clin Microbiol"},{"key":"ref19","first-page":"789","article-title":"[Preliminary report of an epidemic tularemia outbreak in Valladolid].","volume":"198","author":"P Bachiller Luque","year":"1998","journal-title":"Rev Clin Esp"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1007\/s100960050462","article-title":"Report on five cases of tularaemic pneumonia in a tularaemia outbreak in Spain.","volume":"19","author":"J Bellido-Casado","year":"2000","journal-title":"Eur J Clin Microbiol Infect Dis"},{"key":"ref21","first-page":"418","article-title":"An outbreak of tularemia in Castilla-Leon. First isolation of <italic>Francisella tularensis<\/italic> in Spain.","volume":"15","author":"FJ Garc\u00eda Pe\u0148a","year":"1998","journal-title":"Medicina Veterinaria"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1086\/322601","article-title":"Tularemia epidemic in northwestern Spain: clinical description and therapeutic response.","volume":"33","author":"JL Perez-Castrillon","year":"2001","journal-title":"Clin Infect Dis"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"2964","DOI":"10.1128\/JCM.40.8.2964-2972.2002","article-title":"Genotyping of <italic>Francisella tularensis<\/italic> strains by pulsed-field gel electrophoresis, amplified fragment length polymorphism fingerprinting, and 16S rRNA gene sequencing.","volume":"40","author":"N Garcia Del Blanco","year":"2002","journal-title":"J Clin Microbiol"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1136\/vr.154.2.55","article-title":"Biochemical characterisation of <italic>Francisella tularensis<\/italic> strains isolated in Spain.","volume":"154","author":"N Garcia del Blanco","year":"2004","journal-title":"Vet Rec"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"2774","DOI":"10.1128\/JCM.41.6.2774-2776.2003","article-title":"<italic>Francisella tularensis<\/italic> bacteremia.","volume":"41","author":"X Haristoy","year":"2003","journal-title":"J Clin Microbiol"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"e3427","DOI":"10.1371\/journal.pone.0003427","article-title":"Re-assembly of the genome of <italic>Francisella tularensis<\/italic> subsp. <italic>holarctica<\/italic> OSU18.","volume":"3","author":"D Puiu","year":"2008","journal-title":"PLoS ONE"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1016\/S0966-842X(01)02131-X","article-title":"The evolution of mycobacterial pathogenicity: clues from comparative genomics.","volume":"9","author":"R Brosch","year":"2001","journal-title":"Trends Microbiol"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"13826","DOI":"10.1073\/pnas.0404012101","article-title":"Insights into the evolution of <italic>Yersinia pestis<\/italic> through whole-genome comparison with <italic>Yersinia pseudotuberculosis<\/italic>.","volume":"101","author":"PS Chain","year":"2004","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"1980","DOI":"10.1128\/IAI.68.4.1980-1987.2000","article-title":"Characterization of heat-inducible expression and cloning of HtpG (Hsp90 homologue) of <italic>Porphyromonas gingivalis<\/italic>.","volume":"68","author":"DE Lopatin","year":"2000","journal-title":"Infect Immun"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"6037","DOI":"10.1073\/pnas.0609675104","article-title":"In vivo negative selection screen identifies genes required for <italic>Francisella<\/italic> virulence.","volume":"104","author":"DS Weiss","year":"2007","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref31","first-page":"2.4.1","article-title":"Preparation of Genomic DNA from Bacteria.","author":"K Wilson","year":"1997"},{"key":"ref32","article-title":"Biosafety in Microbiological and Biomedical Laboratories (BMBL) 4th Edition. 4th ed.","year":"1999"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"4636","DOI":"10.1093\/nar\/27.23.4636","article-title":"Improved microbial gene identification with GLIMMER.","volume":"27","author":"AL Delcher","year":"1999","journal-title":"Nucleic Acids Res"},{"key":"ref34","article-title":"Manatee Home Page.","year":"2006"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"955","DOI":"10.1093\/nar\/25.5.0955","article-title":"tRNAscan-SE: a program for improved detection of transfer RNA genes in genomic sequence.","volume":"25","author":"TM Lowe","year":"1997","journal-title":"Nucleic Acids Res"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1186\/1471-2105-6-31","article-title":"Automated generation of heuristics for biological sequence comparison.","volume":"6","author":"GS Slater","year":"2005","journal-title":"BMC Bioinformatics"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"D121","DOI":"10.1093\/nar\/gki081","article-title":"Rfam: annotating non-coding RNAs in complete genomes.","volume":"33","author":"S Griffiths-Jones","year":"2005","journal-title":"Nucleic Acids Res"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"3389","DOI":"10.1093\/nar\/25.17.3389","article-title":"Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.","volume":"25","author":"SF Altschul","year":"1997","journal-title":"Nucleic Acids Res"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1093\/nar\/28.1.263","article-title":"The Pfam protein families database.","volume":"28","author":"A Bateman","year":"2000","journal-title":"Nucleic Acids Res"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1093\/nar\/29.1.41","article-title":"TIGRFAMs: a protein family resource for the functional identification of proteins.","volume":"29","author":"DH Haft","year":"2001","journal-title":"Nucleic Acids Res"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1006\/jmbi.2000.4315","article-title":"Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes.","volume":"305","author":"A Krogh","year":"2001","journal-title":"J Mol Biol"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1016\/j.jmb.2004.05.028","article-title":"Improved prediction of signal peptides: SignalP 3.0.","volume":"340","author":"JD Bendtsen","year":"2004","journal-title":"J Mol Biol"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1186\/1471-2105-4-41","article-title":"The COG database: an updated version includes eukaryotes.","volume":"4","author":"RL Tatusov","year":"2003","journal-title":"BMC Bioinformatics"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0007041","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,22]],"date-time":"2020-05-22T06:24:14Z","timestamp":1590128654000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0007041"}},"subtitle":[],"editor":[{"given":"Niyaz","family":"Ahmed","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2009,9,16]]},"references-count":43,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2009,9,16]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0007041","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,9,16]]}}}