{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T18:02:26Z","timestamp":1790704946305,"version":"4.1.0"},"reference-count":48,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2010,4,1]],"date-time":"2010-04-01T00:00:00Z","timestamp":1270080000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0009986","type":"journal-article","created":{"date-parts":[[2010,4,1]],"date-time":"2010-04-01T19:28:26Z","timestamp":1270150106000},"page":"e9986","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":39,"title":["Rapid Accumulation of Virulent Rift Valley Fever Virus in Mice from an Attenuated Virus Carrying a Single Nucleotide Substitution in the M RNA"],"prefix":"10.1371","volume":"5","author":[{"given":"John C.","family":"Morrill","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tetsuro","family":"Ikegami","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoko","family":"Yoshikawa-Iwata","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nandadeva","family":"Lokugamage","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sungyong","family":"Won","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaori","family":"Terasaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aya","family":"Zamoto-Niikura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. J.","family":"Peters","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinji","family":"Makino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2010,4,1]]},"reference":[{"key":"ref1","first-page":"1741","article-title":"Bunyaviruses.","author":"C Schmaljohn","year":"2007"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/S0924-8579(02)00295-9","article-title":"Rift Valley fever: an uninvited zoonosis in the Arabian peninsula.","volume":"21","author":"HH Balkhy","year":"2003","journal-title":"Int J Antimicrob Agents"},{"key":"ref3","article-title":"Rift Valley fever","author":"CJ Peters","year":"1989"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"618","DOI":"10.1016\/0035-9203(79)90004-X","article-title":"The Rift Valley fever epizootic in Egypt 1977-78. 1. Description of the epizootic and virological studies.","volume":"73","author":"JM Meegan","year":"1979","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1016\/S0923-2516(06)80134-2","article-title":"Rift Valley fever epizootic in the central highlands of Madagascar.","volume":"143","author":"J Morvan","year":"1992","journal-title":"Res Virol"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"1415","DOI":"10.3201\/eid0812.020195","article-title":"Genetic analysis of viruses associated with emergence of Rift Valley fever in Saudi Arabia and Yemen, 2000-01.","volume":"8","author":"T Shoemaker","year":"2002","journal-title":"Emerg Infect Dis"},{"key":"ref7","first-page":"73","article-title":"Rift Valley fever outbreak- Kenya, November 2006-January 2007.","volume":"56","year":"2007","journal-title":"Morb Mortal Wkly Rep"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"13335","DOI":"10.1128\/JVI.01238-07","article-title":"NSm protein of Rift Valley fever virus suppresses virus-induced apoptosis.","volume":"81","author":"S Won","year":"2007","journal-title":"J Virol"},{"key":"ref9","first-page":"541","article-title":"TFIIH transcription factor, a target for the Rift Valley hemorrhagic fever virus.","volume":"116","author":"N Le May","year":"2004"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"e13","DOI":"10.1371\/journal.ppat.0040013","article-title":"A SAP30 complex inhibits IFN-beta expression in Rift Valley fever virus infected cells.","volume":"4","author":"N Le May","year":"2008","journal-title":"PLoS Pathog"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"e1000287","DOI":"10.1371\/journal.ppat.1000287","article-title":"Rift Valley fever virus NSs protein promotes post-transcriptional downregulation of protein kinase PKR and inhibits eIF2alpha phosphorylation.","volume":"5","author":"T Ikegami","year":"2009","journal-title":"PLoS Pathog"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"4365","DOI":"10.1128\/JVI.02148-08","article-title":"NSs protein of Rift Valley Fever Virus induces the specific degradation of the double-stranded RNA-dependent protein kinase.","volume":"83","author":"M Habjan","year":"2009","journal-title":"J Virol"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1016\/0264-410X(90)90096-5","article-title":"Use of reassortant viruses to map attenuating and temperature-sensitive mutations of the Rift Valley fever virus MP-12 vaccine.","volume":"8","author":"JF Saluzzo","year":"1990","journal-title":"Vaccine"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"405","DOI":"10.4269\/ajtmh.1995.53.405","article-title":"Characterization of clone 13, a naturally attenuated avirulent isolate of Rift Valley fever virus, which is altered in the small segment.","volume":"53","author":"R Muller","year":"1995","journal-title":"Am J Trop Med Hyg"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"1538","DOI":"10.1128\/JVI.74.3.1538-1543.2000","article-title":"The S segment of rift valley fever phlebovirus (Bunyaviridae) carries determinants for attenuation and virulence in mice.","volume":"74","author":"P Vialat","year":"2000","journal-title":"J Virol"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"2681","DOI":"10.1128\/JVI.02501-07","article-title":"Rift valley fever virus lacking the NSs and NSm genes is highly attenuated, confers protective immunity from virulent virus challenge, and allows for differential identification of infected and vaccinated animals.","volume":"82","author":"BH Bird","year":"2008","journal-title":"J Virol"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1016\/j.virol.2008.05.033","article-title":"RNA polymerase I-mediated expression of viral RNA for the rescue of infectious virulent and avirulent Rift Valley fever viruses.","volume":"378","author":"A Billecocq","year":"2008","journal-title":"Virology"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.virol.2007.01.046","article-title":"Rift Valley fever virus lacking NSm proteins retains high virulence in vivo and may provide a model of human delayed onset neurologic disease.","volume":"362","author":"BH Bird","year":"2007","journal-title":"Virology"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"2805","DOI":"10.1128\/JVI.02095-06","article-title":"Complete genome analysis of 33 ecologically and biologically diverse Rift Valley fever virus strains reveals widespread virus movement and low genetic diversity due to recent common ancestry.","volume":"81","author":"BH Bird","year":"2007","journal-title":"J Virol"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1038\/nature04388","article-title":"Quasispecies diversity determines pathogenesis through cooperative interactions in a viral population.","volume":"439","author":"M Vignuzzi","year":"2006","journal-title":"Nature"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"8271","DOI":"10.1128\/JVI.78.15.8271-8280.2004","article-title":"Common E protein determinants for attenuation of glycosaminoglycan-binding variants of Japanese encephalitis and West Nile viruses.","volume":"78","author":"E Lee","year":"2004","journal-title":"J Virol"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"4901","DOI":"10.1128\/JVI.76.10.4901-4911.2002","article-title":"Mechanism of virulence attenuation of glycosaminoglycan-binding variants of Japanese encephalitis virus and Murray Valley encephalitis virus.","volume":"76","author":"E Lee","year":"2002","journal-title":"J Virol"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"2791","DOI":"10.1099\/vir.0.82164-0","article-title":"Virulence attenuation of Dengue virus due to augmented glycosaminoglycan-binding affinity and restriction in extraneural dissemination.","volume":"87","author":"E Lee","year":"2006","journal-title":"J Gen Virol"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"1204","DOI":"10.1128\/JVI.77.2.1204-1210.2003","article-title":"Glycosaminoglycan binding properties of natural venezuelan equine encephalitis virus isolates.","volume":"77","author":"E Wang","year":"2003","journal-title":"J Virol"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/S0042-6822(02)00097-1","article-title":"Role of heparan sulfate for attachment and entry of tick-borne encephalitis virus.","volume":"308","author":"H Kroschewski","year":"2003","journal-title":"Virology"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"7349","DOI":"10.1128\/JVI.72.9.7349-7356.1998","article-title":"Binding of Sindbis virus to cell surface heparan sulfate.","volume":"72","author":"AP Byrnes","year":"1998","journal-title":"J Virol"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"2271","DOI":"10.1099\/0022-1317-66-10-2271","article-title":"Mutagen-directed attenuation of Rift Valley fever virus as a method for vaccine development.","volume":"66 (Pt 10)","author":"H Caplen","year":"1985","journal-title":"J Gen Virol"},{"key":"ref28","first-page":"21","article-title":"Pathogenesis of Rift Valley fever.","volume":"3","author":"CJ Peters","year":"1981","journal-title":"Contributions to Epidemiology & Biostatistics"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"1104","DOI":"10.2460\/ajvr.1997.58.10.1104","article-title":"Safety and efficacy of a mutagen-attenuated Rift Valley fever virus vaccine in cattle.","volume":"58","author":"JC Morrill","year":"1997","journal-title":"Am J Vet Res"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"8098","DOI":"10.1073\/pnas.85.21.8098","article-title":"Evolution to predominance of swine influenza virus hemagglutinin mutants of predictable phenotype during single infections of the natural host.","volume":"85","author":"ED Kilbourne","year":"1988","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"10465","DOI":"10.1128\/JVI.00825-08","article-title":"Hidden virulence determinants in a viral quasispecies in vivo.","volume":"82","author":"M Sanz-Ramos","year":"2008","journal-title":"J Virol"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/j.gene.2006.07.037","article-title":"Minority memory genomes can influence the evolution of HIV-1 quasispecies in vivo.","volume":"384","author":"C Briones","year":"2006","journal-title":"Gene"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"1309","DOI":"10.1099\/vir.0.83662-0","article-title":"Quasispecies dynamics and fixation of a synonymous mutation in hantavirus transmission.","volume":"89","author":"T Sironen","year":"2008","journal-title":"J Gen Virol"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1016\/0042-6822(89)90365-6","article-title":"Baculovirus expression of the M genome segment of Rift Valley fever virus and examination of antigenic and immunogenic properties of the expressed proteins.","volume":"170","author":"CS Schmaljohn","year":"1989","journal-title":"Virology"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1007\/BF01316748","article-title":"Topological mapping of antigenic sites on the Rift Valley fever virus envelope glycoproteins using monoclonal antibodies.","volume":"121","author":"TG Besselaar","year":"1991","journal-title":"Arch Virol"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"4657","DOI":"10.1016\/j.vaccine.2005.08.034","article-title":"Immunogenicity of combination DNA vaccines for Rift Valley fever virus, tick-borne encephalitis virus, Hantaan virus, and Crimean Congo hemorrhagic fever virus.","volume":"24","author":"K Spik","year":"2006","journal-title":"Vaccine"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1016\/0882-4010(87)90126-4","article-title":"Pathogenesis of Rift Valley fever virus (RVFV) in inbred rats.","volume":"2","author":"GW Anderson Jr","year":"1987","journal-title":"Microb Pathog"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"1559","DOI":"10.2460\/ajvr.1980.41.10.1559","article-title":"Evaluation of a formalin-inactivated Rift Valley fever vaccine in sheep.","volume":"41","author":"DG Harrington","year":"1980","journal-title":"Am J Vet Res"},{"key":"ref39","first-page":"197","article-title":"Active and passive immunization against Rift Valley fever virus infection in Syrian hamsters.","volume":"92","author":"BS Niklasson","year":"1984","journal-title":"Acta Pathol Microbiol Immunol Scand [C]"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1007\/BF01311021","article-title":"Experimental Rift Valley fever in rhesus macaques.","volume":"99","author":"CJ Peters","year":"1988","journal-title":"Arch Virol"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1016\/0166-3542(86)90024-0","article-title":"Prophylaxis of Rift Valley fever with antiviral drugs, immune serum, an interferon inducer, and a macrophage activator.","volume":"6","author":"CJ Peters","year":"1986","journal-title":"Antiviral Res"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/S0264-410X(99)00218-2","article-title":"Immunogenicity of an inactivated Rift Valley fever vaccine in humans: a 12-year experience.","volume":"18","author":"PR Pittman","year":"1999","journal-title":"Vaccine"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1128\/JVI.58.2.263-270.1986","article-title":"Use of bacterial expression cloning to define the amino acid sequences of antigenic determinants on the G2 glycoprotein of Rift Valley fever virus.","volume":"58","author":"K Keegan","year":"1986","journal-title":"J Virol"},{"key":"ref44","first-page":"in press","article-title":"NSm protein of Rift Valley fever virus suppresses virus-induced apoptosis.","volume":"81","author":"A Won","year":"2007","journal-title":"J Virol"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"2933","DOI":"10.1128\/JVI.80.6.2933-2940.2006","article-title":"Rescue of infectious Rift Valley fever virus entirely from cDNA, analysis of virus lacking NSsgene, and expression of a foreign gene.","volume":"80","author":"T Ikegami","year":"2006","journal-title":"J Virol"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"5606","DOI":"10.1128\/JVI.79.9.5606-5615.2005","article-title":"Rift Valley fever virus nonstructural protein NSs promotes viral RNA replication and transcription in a minigenome system.","volume":"79","author":"T Ikegami","year":"2005","journal-title":"J Virol"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"1162","DOI":"10.1128\/JVI.01702-06","article-title":"Severe acute respiratory syndrome coronavirus infection of mice transgenic for the human Angiotensin-converting enzyme 2 virus receptor.","volume":"81","author":"CT Tseng","year":"2007","journal-title":"J Virol"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"8274","DOI":"10.1128\/JVI.00476-06","article-title":"NSm and 78-kilodalton proteins of Rift Valley fever virus are nonessential for viral replication in cell culture.","volume":"80","author":"S Won","year":"2006","journal-title":"J Virol"}],"updated-by":[{"DOI":"10.1371\/annotation\/326c5f5b-3da9-4b0b-b889-28d4f33d2543","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2010,4,13]],"date-time":"2010-04-13T00:00:00Z","timestamp":1271116800000}}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0009986","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,19]],"date-time":"2025-02-19T20:34:32Z","timestamp":1739997272000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0009986"}},"subtitle":[],"editor":[{"given":"Dong-Yan","family":"Jin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2010,4,1]]},"references-count":48,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2010,4,1]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0009986","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,4,1]]}}}