{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T01:03:03Z","timestamp":1773277383027,"version":"3.50.1"},"reference-count":45,"publisher":"Public Library of Science (PLoS)","issue":"8","license":[{"start":{"date-parts":[[2010,8,10]],"date-time":"2010-08-10T00:00:00Z","timestamp":1281398400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0012085","type":"journal-article","created":{"date-parts":[[2010,8,10]],"date-time":"2010-08-10T19:25:20Z","timestamp":1281468320000},"page":"e12085","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":14,"title":["Chitosan Modification of Adenovirus to Modify Transfection Efficiency in Bovine Corneal Epithelial Cells"],"prefix":"10.1371","volume":"5","author":[{"given":"I-Jong","family":"Wang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min-Chao","family":"Jhuang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu-Hsin","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lung-Kun","family":"Yeh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chia-Yang","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tai-Horng","family":"Young","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2010,8,10]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1016\/j.preteyeres.2005.04.001","article-title":"Gene therapy in the cornea.","volume":"24","author":"RR Mohan","year":"2005","journal-title":"Prog Retin Eye Res"},{"key":"ref2","first-page":"614","article-title":"An Sv40-Immortalized Human Corneal Epithelial-Cell Line and Its Characterization.","volume":"36","author":"K Arakisasaki","year":"1995","journal-title":"Investigative Ophthalmology & Visual Science"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1002\/jgm.210","article-title":"Non-invasive observation of repeated adenoviral GFP gene delivery to the anterior segment of the monkey eye in vivo.","volume":"3","author":"T Borras","year":"2001","journal-title":"Journal of Gene Medicine"},{"key":"ref4","first-page":"1282","article-title":"Ocular adenovirus gene transfer varies in efficiency and inflammatory response.","volume":"37","author":"T Borras","year":"1996","journal-title":"Investigative Ophthalmology & Visual Science"},{"key":"ref5","first-page":"2211","article-title":"In-Vivo Gene-Transfer into Murine Corneal Endothelial and Trabecular Meshwork Cells.","volume":"36","author":"DL Budenz","year":"1995","journal-title":"Investigative Ophthalmology & Visual Science"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"2194","DOI":"10.1167\/iovs.03-1224","article-title":"In vivo gene delivery and visualization of corneal stromal cells using an adenoviral vector and keratocyte-specific promoter.","volume":"45","author":"EC Carlson","year":"2004","journal-title":"Investigative Ophthalmology & Visual Science"},{"key":"ref7","first-page":"122","article-title":"In-Vivo Adenovirus-Mediated Gene-Transfer into Ocular-Tissues.","volume":"1","author":"B Mashhour","year":"1994","journal-title":"Gene Therapy"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1006\/exer.1998.0557","article-title":"Adenovirus-mediated gene transfer to the ocular surface epithelium.","volume":"67","author":"K Tsubota","year":"1998","journal-title":"Experimental Eye Research"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"2605","DOI":"10.1099\/0022-1317-81-11-2605","article-title":"Blood clearance rates of adenovirus type 5 in mice.","volume":"81","author":"R Alemany","year":"2000","journal-title":"Journal of General Virology"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"1887","DOI":"10.1089\/104303402760372972","article-title":"PEGylation of E1-deleted adenovirus vectors allows significant gene expression on readministration to liver.","volume":"13","author":"MA Croyle","year":"2002","journal-title":"Human Gene Therapy"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"1713","DOI":"10.1089\/10430340050111368","article-title":"Development of a rapid method for the PEGylation of adenoviruses with enhanced transduction and improved stability under harsh storage conditions.","volume":"11","author":"MA Croyle","year":"2000","journal-title":"Human Gene Therapy"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.ymthe.2004.09.015","article-title":"Evaluation of polyethylene glycol modification of first-generation and helper-dependent adenoviral vectors to reduce innate immune responses.","volume":"11","author":"H Mok","year":"2005","journal-title":"Mol Ther"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"995","DOI":"10.1038\/sj.gt.3300665","article-title":"Adenovirus complexed with polyethylene glycol and cationic lipid is shielded from neutralizing antibodies in vitro.","volume":"5","author":"M Chillon","year":"1998","journal-title":"Gene Therapy"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"604","DOI":"10.1002\/jgm.699","article-title":"PEGylated adenovirus vectors containing RGD peptides on the tip of PEG show high transduction efficiency and antibody evasion ability.","volume":"7","author":"Y Eto","year":"2005","journal-title":"J Gene Med"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"1349","DOI":"10.1089\/10430349950018021","article-title":"PEGylation of adenovirus with retention of infectivity and protection from neutralizing antibody in vitro and in vivo.","volume":"10","author":"CR O'Riordan","year":"1999","journal-title":"Human Gene Therapy"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"4792","DOI":"10.1128\/JVI.75.10.4792-4801.2001","article-title":"\u201cStealth\u201d adenoviruses blunt cell-mediated and humoral immune responses against the virus and allow for significant gene expression upon readministration in the lung.","volume":"75","author":"MA Croyle","year":"2001","journal-title":"J Virol"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/S1525-0016(03)00139-4","article-title":"Targeting adenoviral vectors using heterofunctional polyethylene glycol FGF2 conjugates.","volume":"8","author":"J Lanciotti","year":"2003","journal-title":"Molecular Therapy"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1089\/10430340460745766","article-title":"A novel strategy to modify adenovirus tropism and enhance transgene delivery to activated vascular endothelial cells in vitro and in vivo.","volume":"15","author":"KI Ogawara","year":"2004","journal-title":"Human Gene Therapy"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"356","DOI":"10.1038\/sj.gt.3302655","article-title":"Chick embryo lethal orphan virus can be polymer-coated and retargeted to infect mammalian cells.","volume":"13","author":"M Stevenson","year":"2006","journal-title":"Gene Therapy"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"1256","DOI":"10.1038\/sj.gt.3302295","article-title":"Extended plasma circulation time and decreased toxicity of polymer-coated adenovirus.","volume":"11","author":"NK Green","year":"2004","journal-title":"Gene Ther"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1038\/sj.gt.3301389","article-title":"Polymer-coated adenovirus permits efficient retargeting and evades neutralising antibodies.","volume":"8","author":"KD Fisher","year":"2001","journal-title":"Gene Ther"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1038\/sj.cgt.7701022","article-title":"Incorporation of a laminin-derived peptide (SIKVAV) on polymer-modified adenovirus permits tumor-specific targeting via alpha6-integrins.","volume":"14","author":"M Stevenson","year":"2007","journal-title":"Cancer Gene Ther"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"1036","DOI":"10.1042\/bst0311036","article-title":"Mucoadhesive interactions.","volume":"31","author":"SE Harding","year":"2003","journal-title":"Biochem Soc Trans"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/0168-3659(95)00146-8","article-title":"The potential of mucoadhesive polymers in enhancing intestinal peptide drug absorption .3. Effects of chitosan-glutamate and carbomer on epithelial tight junctions in vitro.","volume":"39","author":"G Borchard","year":"1996","journal-title":"Journal of Controlled Release"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1006\/mthe.2001.0411","article-title":"Development of novel formulations that enhance adenoviral-mediated gene expression in the lung in vitro and in vivo.","volume":"4","author":"MA Croyle","year":"2001","journal-title":"Mol Ther"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"4573","DOI":"10.1016\/S0142-9612(02)00203-X","article-title":"Receptor-independent augmentation of adenovirus-mediated gene transfer with chitosan in vitro.","volume":"23","author":"Y Kawamata","year":"2002","journal-title":"Biomaterials"},{"key":"ref27","first-page":"2614","article-title":"ETB and epidermal growth factor receptor stimulation of wound closure in bovine corneal epithelial cells.","volume":"36","author":"W Tao","year":"1995","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1128\/AEM.18.2.268-269.1969","article-title":"Preparation of primary bovine corneal epithelial cell cultures for use in virological investigations.","volume":"18","author":"GE Wilcox","year":"1969","journal-title":"Appl Microbiol"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/S0378-5173(03)00271-0","article-title":"Thiolated polymers\u2013thiomers: synthesis and in vitro evaluation of chitosan-2-iminothiolane conjugates.","volume":"260","author":"A Bernkop-Schnurch","year":"2003","journal-title":"Int J Pharm"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"880","DOI":"10.1021\/bm049352e","article-title":"Thiolation of chitosan. Attachment of proteins via thioether formation.","volume":"6","author":"T Masuko","year":"2005","journal-title":"Biomacromolecules"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"2589","DOI":"10.1002\/j.1460-2075.1993.tb05919.x","article-title":"Difference imaging of adenovirus: bridging the resolution gap between X-ray crystallography and electron microscopy.","volume":"12","author":"PL Stewart","year":"1993","journal-title":"Embo J"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"2086","DOI":"10.1016\/S0022-2275(20)32507-4","article-title":"A comparison of LDL size determination using gradient gel electrophoresis and light-scattering methods.","volume":"39","author":"D O'Neal","year":"1998","journal-title":"Journal of Lipid Research"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"986","DOI":"10.1128\/AEM.20.6.986-988.1970","article-title":"Technique for viral neutralization antibody surveys in primary microcultures.","volume":"20","author":"RJ Helmke","year":"1970","journal-title":"Applied Microbiology"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/S0928-0987(03)00178-7","article-title":"The effect of a PEG versus a chitosan coating on the interaction of drug colloidal carriers with the ocular mucosa.","volume":"20","author":"AM De Campos","year":"2003","journal-title":"European Journal of Pharmaceutical Sciences"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"1451","DOI":"10.1211\/0022357022476","article-title":"The potential of chitosan in ocular drug delivery.","volume":"55","author":"MJ Alonso","year":"2003","journal-title":"J Pharm Pharmacol"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"1416","DOI":"10.1167\/iovs.05-0495","article-title":"Chitosan nanoparticles as a potential drug delivery system for the ocular surface: toxicity, uptake mechanism and in vivo tolerance.","volume":"47","author":"A Enriquez de Salamanca","year":"2006","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"1553","DOI":"10.1016\/j.biomaterials.2006.11.028","article-title":"Ocular drug delivery by liposome-chitosan nanoparticle complexes (LCS-NP).","volume":"28","author":"Y Diebold","year":"2007","journal-title":"Biomaterials"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"803","DOI":"10.1023\/B:PHAM.0000026432.75781.cb","article-title":"Chitosan nanoparticles as new ocular drug delivery systems: in vitro stability, in vivo fate, and cellular toxicity.","volume":"21","author":"AM De Campos","year":"2004","journal-title":"Pharm Res"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1038\/sj.gt.3302441","article-title":"PEGylated helper-dependent adenoviral vectors: highly efficient vectors with an enhanced safety profile.","volume":"12","author":"MA Croyle","year":"2005","journal-title":"Gene Ther"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/j.ymthe.2005.03.006","article-title":"Combined genetic and chemical capsid modifications enable flexible and efficient de- and retargeting of adenovirus vectors.","volume":"12","author":"F Kreppel","year":"2005","journal-title":"Mol Ther"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1002\/jgm.853","article-title":"Photochemically enhanced transduction of polymer-complexed adenovirus targeted to the epidermal growth factor receptor.","volume":"8","author":"A Bonsted","year":"2006","journal-title":"J Gene Med"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1038\/sj.gt.3300349","article-title":"Polycations increase the efficiency of adenovirus-mediated gene transfer to epithelial and endothelial cells in vitro.","volume":"4","author":"SM Arcasoy","year":"1997","journal-title":"Gene Ther"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"6479","DOI":"10.1074\/jbc.272.10.6479","article-title":"Complexes of adenovirus with polycationic polymers and cationic lipids increase the efficiency of gene transfer in vitro and in vivo.","volume":"272","author":"A Fasbender","year":"1997","journal-title":"J Biol Chem"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"422","DOI":"10.1165\/ajrcmb.17.4.2714","article-title":"MUC1 and other sialoglycoconjugates inhibit adenovirus-mediated gene transfer to epithelial cells.","volume":"17","author":"SM Arcasoy","year":"1997","journal-title":"Am J Respir Cell Mol Biol"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1097\/00004397-200404430-00010","article-title":"Gene therapy of the corneal epithelium.","volume":"44","author":"MI Rosenblatt","year":"2004","journal-title":"International Ophthalmology Clinics"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0012085","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,4]],"date-time":"2021-11-04T13:23:12Z","timestamp":1636032192000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0012085"}},"subtitle":[],"editor":[{"given":"Neeraj","family":"Vij","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2010,8,10]]},"references-count":45,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2010,8,10]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0012085","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,8,10]]}}}