{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T14:41:41Z","timestamp":1793716901330,"version":"4.6.1"},"reference-count":43,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2010,10,8]],"date-time":"2010-10-08T00:00:00Z","timestamp":1286496000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0013247","type":"journal-article","created":{"date-parts":[[2010,10,8]],"date-time":"2010-10-08T18:59:17Z","timestamp":1286564357000},"page":"e13247","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":502,"title":["Let-7 MicroRNA Family Is Selectively Secreted into the Extracellular Environment via Exosomes in a Metastatic Gastric Cancer Cell Line"],"prefix":"10.1371","volume":"5","author":[{"given":"Keiichi","family":"Ohshima","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kanako","family":"Inoue","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akemi","family":"Fujiwara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keiichi","family":"Hatakeyama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaori","family":"Kanto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuko","family":"Watanabe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koji","family":"Muramatsu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yorikane","family":"Fukuda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shun-ichiro","family":"Ogura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ken","family":"Yamaguchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tohru","family":"Mochizuki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2010,10,8]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1038\/nri2567","article-title":"Membrane vesicles as conveyors of immune responses.","volume":"9","author":"C Th\u00e9ry","year":"2009","journal-title":"Nat Rev Immunol"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"654","DOI":"10.1038\/ncb1596","article-title":"Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.","volume":"9","author":"H Valadi","year":"2007","journal-title":"Nat Cell Biol"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"1470","DOI":"10.1038\/ncb1800","article-title":"Glioblastoma microvesicles transport RNA and proteins that promote tumour growth and provide diagnostic biomarkers.","volume":"10","author":"J Skog","year":"2008","journal-title":"Nat Cell Biol"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1038\/ncb1725","article-title":"Intercellular transfer of the oncogenic receptor EGFRvIII by microvesicles derived from tumour cells.","volume":"10","author":"K Al-Nedawi","year":"2008","journal-title":"Nat Cell Biol"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1038\/nri2020","article-title":"Intercellular transfer of cell-surface proteins is common and can affect many stages of an immune response.","volume":"7","author":"DM Davis","year":"2007","journal-title":"Nat Rev Immunol"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1038\/nri855","article-title":"Exosomes: composition, biogenesis and function.","volume":"2","author":"C Th\u00e9ry","year":"2002","journal-title":"Nat Rev Immunol"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1586\/epr.09.17","article-title":"Exosomes: proteomic insights and diagnostic potential.","volume":"6","author":"RJ Simpson","year":"2009","journal-title":"Expert Rev Proteomics"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"42","DOI":"10.3816\/CLC.2009.n.006","article-title":"Exosomal microRNA: a diagnostic marker for lung cancer.","volume":"10","author":"G Rabinowits","year":"2009","journal-title":"Clin Lung Cancer"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"13368","DOI":"10.1073\/pnas.0403453101","article-title":"Identification and proteomic profiling of exosomes in human urine.","volume":"101","author":"T Pisitkun","year":"2004","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1111\/j.1601-0825.2009.01604.x","article-title":"Exosomes from human saliva as a source of microRNA biomarkers.","volume":"16","author":"A Michael","year":"2009","journal-title":"Oral Dis"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"1095","DOI":"10.1038\/sj.ki.5002486","article-title":"CD24 is a marker of exosomes secreted into urine and amniotic fluid.","volume":"72","author":"S Keller","year":"2007","journal-title":"Kidney Int"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1016\/S0140-6736(02)09552-1","article-title":"Malignant effusions and immunogenic tumour-derived exosomes.","volume":"360","author":"F Andre","year":"2002","journal-title":"Lancet"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.ygyno.2008.04.033","article-title":"MicroRNA signatures of tumor-derived exosomes as diagnostic biomarkers of ovarian cancer.","volume":"110","author":"DD Taylor","year":"2008","journal-title":"Gynecol Oncol"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1038\/nrg2504","article-title":"Small silencing RNAs: an expanding universe.","volume":"10","author":"M Ghildiyal","year":"2009","journal-title":"Nat Rev Genet"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1586\/14737159.7.5.569","article-title":"Role of miRNA in carcinogenesis and biomarker selection: a methodological view.","volume":"7","author":"FE Ahmed","year":"2007","journal-title":"Expert Rev Mol Diagn"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"704","DOI":"10.1038\/nrg2634","article-title":"Causes and consequences of microRNA dysregulation in cancer.","volume":"10","author":"CM Croce","year":"2009","journal-title":"Nat Rev Genet"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"10513","DOI":"10.1073\/pnas.0804549105","article-title":"Circulating microRNAs as stable blood-based markers for cancer detection.","volume":"105","author":"PS Mitchell","year":"2008","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"997","DOI":"10.1038\/cr.2008.282","article-title":"Characterization of microRNAs in serum: a novel class of biomarkers for diagnosis of cancer and other diseases.","volume":"18","author":"X Chen","year":"2008","journal-title":"Cell Res"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"482","DOI":"10.1373\/clinchem.2007.097972","article-title":"Detection and characterization of placental microRNAs in maternal plasma.","volume":"54","author":"SS Chim","year":"2008","journal-title":"Clin Chem"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"e3148","DOI":"10.1371\/journal.pone.0003148","article-title":"Serum microRNAs are promising novel biomarkers.","volume":"3","author":"S Gilad","year":"2008","journal-title":"PLoS One"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"e3694","DOI":"10.1371\/journal.pone.0003694","article-title":"Detection of microRNA expression in human peripheral blood microvesicles.","volume":"3","author":"MP Hunter","year":"2008","journal-title":"PLoS One"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"672","DOI":"10.1111\/j.1365-2141.2008.07077.x","article-title":"Detection of elevated levels of tumour-associated microRNAs in serum of patients with diffuse large B-cell lymphoma.","volume":"141","author":"CH Lawrie","year":"2008","journal-title":"Br J Haematol"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"e6229","DOI":"10.1371\/journal.pone.0006229","article-title":"Detection of cancer with serum miRNAs on an oligonucleotide microarray.","volume":"4","author":"MJ Lodes","year":"2009","journal-title":"PLoS One"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"e5532","DOI":"10.1371\/journal.pone.0005532","article-title":"Down-regulation of miR-92 in human plasma is a novel marker for acute leukemia patients.","volume":"4","author":"M Tanaka","year":"2009","journal-title":"PLoS One"},{"key":"ref25","article-title":"A robust methodology to study urine microRNA as tumor marker: microRNA-126 and microRNA-182 are related to urinary bladder cancer.","author":"M Hanke","journal-title":"Urol Oncol"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"5473","DOI":"10.1158\/1078-0432.CCR-09-0736","article-title":"Salivary microRNA: discovery, characterization, and clinical utility for oral cancer detection.","volume":"15","author":"NJ Park","year":"2009","journal-title":"Clin Cancer Res"},{"key":"ref27","first-page":"4894","article-title":"Peptidomics-based approach reveals the secretion of the 29-residue COOH-terminal fragment of the putative tumor suppressor protein DMBT1 from pancreatic adenocarcinoma cell lines.","volume":"62","author":"K Sasaki","year":"2002","journal-title":"Cancer Res"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"1620","DOI":"10.1002\/prca.200800039","article-title":"Proneurotensin\/neuromedin N secreted from small cell lung carcinoma cell lines as a potential tumor marker.","volume":"2","author":"S Ogura","year":"2008","journal-title":"Proteomics Clin Appl"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"2660","DOI":"10.1002\/elps.200800015","article-title":"Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.","volume":"29","author":"H Ji","year":"2008","journal-title":"Electrophoresis"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1016\/j.tcb.2008.07.007","article-title":"The let-7 family of microRNAs.","volume":"18","author":"S Roush","year":"2008","journal-title":"Trends in Cell Biology"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"F19","DOI":"10.1677\/ERC-09-0184","article-title":"The role of let-7 in cell differentiation and cancer.","volume":"17","author":"B Boyerinas","year":"2010","journal-title":"Endocr Relat Cancer"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1111\/j.1349-7006.2000.tb01004.x","article-title":"A novel experimental mouse model of peritoneal dissemination of human gastric cancer cells: different mechanisms in peritoneal dissemination and hematogenous metastasis.","volume":"91","author":"H Nishimori","year":"2000","journal-title":"Jpn J Cancer Res"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1016\/j.jss.2005.04.030","article-title":"Metastases-related genes in the classification of liver and peritoneal metastasis in human gastric cancer.","volume":"129","author":"R Fukui","year":"2005","journal-title":"J Surg Res"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1261\/rna.1295509","article-title":"Impact of normalization on miRNA microarray expression profiling.","volume":"15","author":"S Pradervand","year":"2009","journal-title":"RNA"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"e5540","DOI":"10.1371\/journal.pone.0005540","article-title":"Intra-platform repeatability and inter-platform comparability of microRNA microarray technology.","volume":"4","author":"F Sato","year":"2009","journal-title":"PLoS One"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"1419","DOI":"10.1158\/0008-5472.CAN-06-4074","article-title":"The human let-7a-3 locus contains an epigenetically regulated microRNA gene with oncogenic function.","volume":"67","author":"B Brueckner","year":"2007","journal-title":"Cancer Res"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"1215","DOI":"10.1007\/s10495-008-0256-z","article-title":"Let-7a microRNA suppresses therapeutics-induced cancer cell death by targeting caspase-3.","volume":"13","author":"WP Tsang","year":"2008","journal-title":"Apoptosis"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"138","DOI":"10.4049\/jimmunol.134.1.138","article-title":"Membrane vesicles shed by murine melanoma cells selectively inhibit the expression of la antigen by macrophages.","volume":"134","author":"DD Poutsiaka","year":"1985","journal-title":"J Immunol"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"2912","DOI":"10.1158\/0008-5472.CAN-07-0520","article-title":"Tumor-released microvesicles as vesicles of immunosuppression.","volume":"67","author":"R Valenti","year":"2007","journal-title":"Cancer Res"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1186\/1479-5876-6-37","article-title":"Exosomes as a tumor immune escape mechanism: possible therapeutic implications.","volume":"6","author":"TE Ichim","year":"2008","journal-title":"J Transl Med"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1097\/SLA.0b013e3181cc939f","article-title":"Circulating microRNAs as novel minimally invasive biomarkers for breast cancer.","volume":"251","author":"HM Heneghan","year":"2010","journal-title":"Ann Surg"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1002\/ijc.10242","article-title":"Intracellular targeting therapy of cisplatin-encapsulated transferrin-polyethylene glycol liposome on peritoneal dissemination of gastric cancer.","volume":"99","author":"H Iinuma","year":"2002","journal-title":"Int J Cancer"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"2371","DOI":"10.3748\/wjg.v16.i19.2371","article-title":"Effect of 5-aza-2\u2032-deoxycytidine on immune-associated proteins in exosomes from hepatoma.","volume":"21","author":"WH Xiao","year":"2010","journal-title":"World J Gastroenterol"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0013247","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,6,3]],"date-time":"2023-06-03T18:58:20Z","timestamp":1685818700000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0013247"}},"subtitle":[],"editor":[{"given":"Stefan","family":"W\u00f6lfl","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2010,10,8]]},"references-count":43,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2010,10,8]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0013247","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,10,8]]}}}