{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T08:48:02Z","timestamp":1790930882253,"version":"4.1.0"},"reference-count":44,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2010,10,11]],"date-time":"2010-10-11T00:00:00Z","timestamp":1286755200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0013274","type":"journal-article","created":{"date-parts":[[2010,10,11]],"date-time":"2010-10-11T19:41:41Z","timestamp":1286826101000},"page":"e13274","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":15,"title":["Identification of the Essential Brucella melitensis Porin Omp2b as a Suppressor of Bax-Induced Cell Death in Yeast in a Genome-Wide Screening"],"prefix":"10.1371","volume":"5","author":[{"given":"G\u00e9raldine","family":"Laloux","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Micha\u00ebl","family":"Deghelt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie","family":"de Barsy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Jacques","family":"Letesson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xavier","family":"De Bolle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2010,10,11]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1038\/nrmicro1320","article-title":"Actin-dependent movement of bacterial pathogens.","volume":"4","author":"JM Stevens","year":"2006","journal-title":"Nat Rev Microbiol"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"827","DOI":"10.1038\/nature06247","article-title":"Manipulation of host-cell pathways by bacterial pathogens.","volume":"449","author":"AP Bhavsar","year":"2007","journal-title":"Nature"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"E183","DOI":"10.1038\/15620","article-title":"Controlling the maturation of pathogen-containing vacuoles: a matter of life and death.","volume":"1","author":"S Meresse","year":"1999","journal-title":"Nat Cell Biol"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"1339","DOI":"10.1038\/cdd.2008.91","article-title":"Cell death in the host response to infection.","volume":"15","author":"K Labbe","year":"2008","journal-title":"Cell Death Differ"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/j.tim.2008.02.001","article-title":"Staying alive: bacterial inhibition of apoptosis during infection.","volume":"16","author":"CS Faherty","year":"2008","journal-title":"Trends Microbiol"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/S1369-5274(00)00165-X","article-title":"Brucellosis: a worldwide zoonosis.","volume":"4","author":"ML Boschiroli","year":"2001","journal-title":"Curr Opin Microbiol"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1128\/IAI.68.1.342-351.2000","article-title":"In vitro Brucella suis infection prevents the programmed cell death of human monocytic cells.","volume":"68","author":"A Gross","year":"2000","journal-title":"Infect Immun"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"5035","DOI":"10.1128\/IAI.01998-05","article-title":"Brucella melitensis triggers time-dependent modulation of apoptosis and down-regulation of mitochondrion-associated gene expression in mouse macrophages.","volume":"74","author":"Y He","year":"2006","journal-title":"Infect Immun"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"618","DOI":"10.1016\/S1369-5274(99)00031-4","article-title":"Yeast as a tool for apoptosis research.","volume":"2","author":"S Matsuyama","year":"1999","journal-title":"Curr Opin Microbiol"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/j.tcb.2008.01.007","article-title":"How do BCL-2 proteins induce mitochondrial outer membrane permeabilization?","volume":"18","author":"JE Chipuk","year":"2008","journal-title":"Trends Cell Biol"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"1449","DOI":"10.1016\/j.bbamcr.2007.12.013","article-title":"The pleiotropic effects of heterologous Bax expression in yeast.","volume":"1783","author":"CM Khoury","year":"2008","journal-title":"Biochim Biophys Acta"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"9238","DOI":"10.1073\/pnas.91.20.9238","article-title":"Interactions among members of the Bcl-2 protein family analyzed with a yeast two-hybrid system.","volume":"91","author":"T Sato","year":"1994","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1016\/S0076-6879(00)22029-6","article-title":"Assays for studying Bax-induced lethality in the yeast Saccharomyces cerevisiae.","volume":"322","author":"Q Xu","year":"2000","journal-title":"Methods Enzymol"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"1307","DOI":"10.1002\/(SICI)1097-0061(19990930)15:13<1307::AID-YEA455>3.0.CO;2-3","article-title":"A selection system for human apoptosis inhibitors using yeast.","volume":"15","author":"W Greenhalf","year":"1999","journal-title":"Yeast"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1016\/S1097-2765(00)80034-9","article-title":"Bax inhibitor-1, a mammalian apoptosis suppressor identified by functional screening in yeast.","volume":"1","author":"Q Xu","year":"1998","journal-title":"Mol Cell"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"2597","DOI":"10.1073\/pnas.97.6.2597","article-title":"BAR: An apoptosis regulator at the intersection of caspases and Bcl-2 family proteins.","volume":"97","author":"H Zhang","year":"2000","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"1630","DOI":"10.1104\/pp.103.038091","article-title":"Tomato phospholipid hydroperoxide glutathione peroxidase inhibits cell death induced by Bax and oxidative stresses in yeast and plants.","volume":"135","author":"S Chen","year":"2004","journal-title":"Plant Physiol"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"46284","DOI":"10.1074\/jbc.M107375200","article-title":"Vesicle-associated membrane protein of Arabidopsis suppresses Bax-induced apoptosis in yeast downstream of oxidative burst.","volume":"276","author":"A Levine","year":"2001","journal-title":"J Biol Chem"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1046\/j.1365-313x.2000.00690.x","article-title":"AtBI-1, a plant homologue of Bax inhibitor-1, suppresses Bax-induced cell death in yeast and is rapidly upregulated during wounding and pathogen challenge.","volume":"21","author":"P Sanchez","year":"2000","journal-title":"Plant J"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"554","DOI":"10.1046\/j.1365-313X.2003.01982.x","article-title":"Identification of Pseudomonas syringae type III effectors that can suppress programmed cell death in plants and yeast.","volume":"37","author":"Y Jamir","year":"2004","journal-title":"Plant J"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"e1000774","DOI":"10.1371\/journal.ppat.1000774","article-title":"Anaplasma phagocytophilum Ats-1 is imported into host cell mitochondria and interferes with apoptosis induction.","volume":"6","author":"H Niu","year":"2010","journal-title":"PLoS Pathog"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.chom.2008.06.004","article-title":"The Yeast Saccharomyces cerevisiae: a versatile model system for the identification and characterization of bacterial virulence proteins.","volume":"4","author":"KA Siggers","year":"2008","journal-title":"Cell Host Microbe"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"2201","DOI":"10.1101\/gr.2456204","article-title":"Generation of the Brucella melitensis ORFeome version 1.1.","volume":"14","author":"A Dricot","year":"2004","journal-title":"Genome Res"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1042\/bj20030690","article-title":"Response of yeast to the regulated expression of proteins in the Bcl-2 family.","volume":"374","author":"P Polcic","year":"2003","journal-title":"Biochem J"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"4839","DOI":"10.1128\/JB.183.16.4839-4847.2001","article-title":"Molecular, antigenic, and functional analyses of Omp2b porin size variants of Brucella spp.","volume":"183","author":"JY Paquet","year":"2001","journal-title":"J Bacteriol"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"e1000629","DOI":"10.1371\/journal.ppat.1000629","article-title":"Bacterial porin disrupts mitochondrial membrane potential and sensitizes host cells to apoptosis.","volume":"5","author":"V Kozjak-Pavlovic","year":"2009","journal-title":"PLoS Pathog"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1046\/j.1462-5822.2003.00257.x","article-title":"Neisserial PorB is translocated to the mitochondria of HeLa cells infected with Neisseria meningitidis and protects cells from apoptosis.","volume":"5","author":"P Massari","year":"2003","journal-title":"Cell Microbiol"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1093\/emboj\/18.2.339","article-title":"Neisserial porin (PorB) causes rapid calcium influx in target cells and induces apoptosis by the activation of cysteine proteases.","volume":"18","author":"A Muller","year":"1999","journal-title":"EMBO J"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1146\/annurev.biochem.77.061606.160747","article-title":"Protein translocation across the bacterial cytoplasmic membrane.","volume":"77","author":"AJ Driessen","year":"2008","journal-title":"Annu Rev Biochem"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"19996","DOI":"10.1016\/S0021-9258(17)45473-1","article-title":"Eukaryotic and prokaryotic signal peptides direct secretion of a bacterial endoglucanase by mammalian cells.","volume":"265","author":"J Hall","year":"1990","journal-title":"J Biol Chem"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/0168-1656(95)00127-1","article-title":"Secretion of a bacterial protein by mammalian cells.","volume":"43","author":"JM Clement","year":"1995","journal-title":"J Biotechnol"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"3337","DOI":"10.1093\/jxb\/erp167","article-title":"A bacterial signal peptide is functional in plants and directs proteins to the secretory pathway.","volume":"60","author":"L Moeller","year":"2009","journal-title":"J Exp Bot"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1046\/j.1365-2958.2000.01983.x","article-title":"Structure and function of bacterial outer membrane proteins: barrels in a nutshell.","volume":"37","author":"R Koebnik","year":"2000","journal-title":"Mol Microbiol"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"5332","DOI":"10.1093\/emboj\/19.20.5332","article-title":"Targeting of the pro-apoptotic VDAC-like porin (PorB) of Neisseria gonorrhoeae to mitochondria of infected cells.","volume":"19","author":"A Muller","year":"2000","journal-title":"EMBO J"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"2531","DOI":"10.1073\/pnas.0807830106","article-title":"Signals in bacterial beta-barrel proteins are functional in eukaryotic cells for targeting to and assembly in mitochondria.","volume":"106","author":"DM Walther","year":"2009","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"1519","DOI":"10.1021\/pr060636a","article-title":"Extensive cell envelope modulation is associated with virulence in Brucella abortus.","volume":"6","author":"J Lamontagne","year":"2007","journal-title":"J Proteome Res"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"2645","DOI":"10.1101\/gad.1299905","article-title":"Bacterial outer membrane vesicles and the host-pathogen interaction.","volume":"19","author":"MJ Kuehn","year":"2005","journal-title":"Genes Dev"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1111\/j.1462-5822.2005.00609.x","article-title":"Characterization of Brucella abortus lipopolysaccharide macrodomains as mega rafts.","volume":"8","author":"N Lapaque","year":"2006","journal-title":"Cell Microbiol"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"6784","DOI":"10.4049\/jimmunol.162.11.6784","article-title":"Lysosomal accumulation and recycling of lipopolysaccharide to the cell surface of murine macrophages, an in vitro and in vivo study.","volume":"162","author":"C Forestier","year":"1999","journal-title":"J Immunol"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"857","DOI":"10.1002\/1097-0061(20000630)16:9<857::AID-YEA561>3.0.CO;2-B","article-title":"Rapid and reliable protein extraction from yeast.","volume":"16","author":"VV Kushnirov","year":"2000","journal-title":"Yeast"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"784","DOI":"10.1038\/nbt1183-784","article-title":"A Broad Host Range Mobilization System for In Vivo Genetic Engineering: Transposon Mutagenesis in Gram Negative Bacteria.","volume":"1","author":"R Simon","year":"1983","journal-title":"Bio\/technology"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1006\/meth.2001.1190","article-title":"High-throughput yeast two-hybrid assays for large-scale protein interaction mapping.","volume":"24","author":"AJ Walhout","year":"2001","journal-title":"Methods"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"1197","DOI":"10.1016\/0092-8674(91)90042-W","article-title":"Isolation of three novel human cyclins by rescue of G1 cyclin (Cln) function in yeast.","volume":"66","author":"DJ Lew","year":"1991","journal-title":"Cell"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"6494","DOI":"10.1128\/MCB.16.11.6494","article-title":"Structure-function comparisons of the proapoptotic protein Bax in yeast and mammalian cells.","volume":"16","author":"H Zha","year":"1996","journal-title":"Mol Cell Biol"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0013274","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,6,3]],"date-time":"2023-06-03T19:48:49Z","timestamp":1685821729000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0013274"}},"subtitle":[],"editor":[{"given":"Edgardo","family":"Moreno","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2010,10,11]]},"references-count":44,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2010,10,11]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0013274","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,10,11]]}}}