{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T23:57:17Z","timestamp":1791158237817,"version":"4.1.0"},"reference-count":48,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2010,10,29]],"date-time":"2010-10-29T00:00:00Z","timestamp":1288310400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0013773","type":"journal-article","created":{"date-parts":[[2010,10,29]],"date-time":"2010-10-29T19:27:29Z","timestamp":1288380449000},"page":"e13773","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":12,"title":["Protective Efficacy of BCG Overexpressing an L,D-Transpeptidase against M. tuberculosis Infection"],"prefix":"10.1371","volume":"5","author":[{"given":"Scott T.","family":"Nolan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gyanu","family":"Lamichhane","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2010,10,29]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"1861","DOI":"10.1016\/S0140-6736(09)60331-7","article-title":"Epidemiology of antituberculosis drug resistance 2002-07: an updated analysis of the Global Project on Anti-Tuberculosis Drug Resistance Surveillance.","volume":"373","author":"A Wright","year":"2009","journal-title":"Lancet"},{"key":"ref2","article-title":"Global tuberculosis control.","author":"WHO","year":"2009"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1056\/NEJM193810132191504","article-title":"Significance of Latent Forms of Tuberculosis.","volume":"219","author":"JB Amberson","year":"1938","journal-title":"New England Journal of Medicine"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"1860","DOI":"10.1056\/NEJMcp021045","article-title":"Clinical practice. Latent tuberculosis infection.","volume":"347","author":"RM Jasmer","year":"2002","journal-title":"N Engl J Med"},{"key":"ref5","first-page":"729","article-title":"Pathogenesis of a first episode of chronic pulmonary tuberculosis in man: recrudescence of residuals of the primary infection or exogenous reinfection?","volume":"95","author":"WW Stead","year":"1967","journal-title":"Am Rev Respir Dis"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1056\/NEJM198903023200901","article-title":"A prospective study of the risk of tuberculosis among intravenous drug users with human immunodeficiency virus infection.","volume":"320","author":"PA Selwyn","year":"1989","journal-title":"N Engl J Med"},{"key":"ref7","first-page":"S47","article-title":"Impact of the HIV epidemic on the spread of other diseases: the case of tuberculosis.","volume":"14","author":"E Girardi","year":"2000","journal-title":"AIDS"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"4025","DOI":"10.1128\/JB.184.14.4025-4032.2002","article-title":"Microarray analysis of the Mycobacterium tuberculosis transcriptional response to the acidic conditions found in phagosomes.","volume":"184","author":"MA Fisher","year":"2002","journal-title":"J Bacteriol"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1146\/annurev.micro.55.1.139","article-title":"Nonreplicating persistence of mycobacterium tuberculosis.","volume":"55","author":"LG Wayne","year":"2001","journal-title":"Annu Rev Microbiol"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"717","DOI":"10.1046\/j.1365-2958.2002.02779.x","article-title":"Evaluation of a nutrient starvation model of Mycobacterium tuberculosis persistence by gene and protein expression profiling.","volume":"43","author":"JC Betts","year":"2002","journal-title":"Mol Microbiol"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1084\/jem.20030205","article-title":"Inhibition of respiration by nitric oxide induces a Mycobacterium tuberculosis dormancy program.","volume":"198","author":"MI Voskuil","year":"2003","journal-title":"J Exp Med"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"1379","DOI":"10.1128\/IAI.71.3.1379-1388.2003","article-title":"Isolation of acid-inducible genes of Mycobacterium tuberculosis with the use of recombinase-based in vivo expression technology.","volume":"71","author":"B Saviola","year":"2003","journal-title":"Infect Immun"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"7534","DOI":"10.1073\/pnas.121172498","article-title":"Regulation of the Mycobacterium tuberculosis hypoxic response gene encoding alpha -crystallin.","volume":"98","author":"DR Sherman","year":"2001","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"833","DOI":"10.1046\/j.1365-2958.2003.03474.x","article-title":"Rv3133c\/dosR is a transcription factor that mediates the hypoxic response of Mycobacterium tuberculosis.","volume":"48","author":"HD Park","year":"2003","journal-title":"Mol Microbiol"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1128\/CVI.13.2.179-186.2006","article-title":"Recognition of stage-specific mycobacterial antigens differentiates between acute and latent infections with Mycobacterium tuberculosis.","volume":"13","author":"A Demissie","year":"2006","journal-title":"Clin Vaccine Immunol"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"1203","DOI":"10.1128\/CVI.00111-09","article-title":"Immunogenicity of novel DosR regulon-encoded candidate antigens of Mycobacterium tuberculosis in three high-burden populations in Africa.","volume":"16","author":"GF Black","year":"2009","journal-title":"Clin Vaccine Immunol"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"4360","DOI":"10.1128\/JB.00239-08","article-title":"The peptidoglycan of stationary-phase Mycobacterium tuberculosis predominantly contains cross-links generated by L,D-transpeptidation.","volume":"190","author":"M Lavollay","year":"2008","journal-title":"J Bacteriol"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"466","DOI":"10.1038\/nm.2120","article-title":"The Mycobacterium tuberculosis protein Ldt(Mt2) is a nonclassical transpeptidase required for virulence and resistance to amoxicillin.","volume":"16","author":"R Gupta","year":"2010","journal-title":"Nat Med"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1038\/nm.2087","article-title":"Recognition of peptidoglycan from the microbiota by Nod1 enhances systemic innate immunity.","volume":"16","author":"TB Clarke","year":"2010","journal-title":"Nat Med"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1016\/j.vaccine.2005.07.078","article-title":"Preclinical testing of new vaccines for tuberculosis: a comprehensive review.","volume":"24","author":"IM Orme","year":"2006","journal-title":"Vaccine"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1016\/S1472-9792(02)00069-0","article-title":"The mouse as a useful model of tuberculosis.","volume":"83","author":"IM Orme","year":"2003","journal-title":"Tuberculosis (Edinb)"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1016\/j.coi.2009.05.017","article-title":"Tuberculosis vaccine research: the impact of immunology.","volume":"21","author":"LF Barker","year":"2009","journal-title":"Curr Opin Immunol"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1038\/351456a0","article-title":"New use of BCG for recombinant vaccines.","volume":"351","author":"CK Stover","year":"1991","journal-title":"Nature"},{"key":"ref24","first-page":"683","article-title":"Differential characteristics in vitro and in vivo of several substrains of BCG. III. Multiplication and survival in vivo.","volume":"74","author":"RJ Dubos","year":"1956","journal-title":"Am Rev Tuberc"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1111\/j.1365-3083.2009.02231.x","article-title":"More vaccine efficacy studies on the recombinant Bacille Calmette-Guerin co-expressing Ag85B, Mpt64 and Mtb8.4.","volume":"69","author":"YQ Qie","year":"2009","journal-title":"Scand J Immunol"},{"key":"ref26","first-page":"xiv, 453 p. p","article-title":"Pathogenesis of human pulmonary tuberculosis : insights from the rabbit model.","author":"AM Dannenberg","year":"2006"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1007\/s10875-007-9084-0","article-title":"Host innate immune response to Mycobacterium tuberculosis.","volume":"27","author":"K Bhatt","year":"2007","journal-title":"J Clin Immunol"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"2243","DOI":"10.1084\/jem.178.6.2243","article-title":"Disseminated tuberculosis in interferon gamma gene-disrupted mice.","volume":"178","author":"AM Cooper","year":"1993","journal-title":"J Exp Med"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"2249","DOI":"10.1084\/jem.178.6.2249","article-title":"An essential role for interferon gamma in resistance to Mycobacterium tuberculosis infection.","volume":"178","author":"JL Flynn","year":"1993","journal-title":"J Exp Med"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"2407","DOI":"10.4049\/jimmunol.141.7.2407","article-title":"Release of reactive nitrogen intermediates and reactive oxygen intermediates from mouse peritoneal macrophages. Comparison of activating cytokines and evidence for independent production.","volume":"141","author":"AH Ding","year":"1988","journal-title":"J Immunol"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"13853","DOI":"10.1073\/pnas.250480397","article-title":"Recombinant bacillus calmette-guerin (BCG) vaccines expressing the Mycobacterium tuberculosis 30-kDa major secretory protein induce greater protective immunity against tuberculosis than conventional BCG vaccines in a highly susceptible animal model.","volume":"97","author":"MA Horwitz","year":"2000","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1038\/nm859","article-title":"Recombinant BCG exporting ESAT-6 confers enhanced protection against tuberculosis.","volume":"9","author":"AS Pym","year":"2003","journal-title":"Nat Med"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"2472","DOI":"10.1172\/JCI24617","article-title":"Increased vaccine efficacy against tuberculosis of recombinant Mycobacterium bovis bacille Calmette-Guerin mutants that secrete listeriolysin.","volume":"115","author":"L Grode","year":"2005","journal-title":"J Clin Invest"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"4412","DOI":"10.1016\/j.vaccine.2009.05.048","article-title":"Novel recombinant BCG expressing perfringolysin O and the over-expression of key immunodominant antigens; pre-clinical characterization, safety and protection against challenge with Mycobacterium tuberculosis.","volume":"27","author":"R Sun","year":"2009","journal-title":"Vaccine"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"1196","DOI":"10.1128\/IAI.73.2.1196-1203.2005","article-title":"Long-term protection against tuberculosis following vaccination with a severely attenuated double lysine and pantothenate auxotroph of Mycobacterium tuberculosis.","volume":"73","author":"VK Sambandamurthy","year":"2005","journal-title":"Infect Immun"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"1491","DOI":"10.1086\/592450","article-title":"A new recombinant bacille Calmette-Guerin vaccine safely induces significantly enhanced tuberculosis-specific immunity in human volunteers.","volume":"198","author":"DF Hoft","year":"2008","journal-title":"J Infect Dis"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"3571","DOI":"10.1016\/j.vaccine.2010.02.094","article-title":"Ag85B-ESAT-6 adjuvanted with IC31 promotes strong and long-lived Mycobacterium tuberculosis specific T cell responses in naive human volunteers.","volume":"28","author":"JT van Dissel","year":"2010","journal-title":"Vaccine"},{"key":"ref38","article-title":"Recombinant BCG tuberculosis vaccine designed to elicit immune responses to Mycobacterium tuberculosis in all physiological stages of infection and disease.","author":"A Shafferman","year":"2010"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1146\/annurev.immunol.19.1.93","article-title":"Immunology of tuberculosis.","volume":"19","author":"JL Flynn","year":"2001","journal-title":"Annu Rev Immunol"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1111\/j.1365-2222.2010.03459.x","article-title":"Attenuated Bordetella pertussis vaccine strain BPZE1 modulates allergen-induced immunity and prevents allergic pulmonary pathology in a murine model.","volume":"40","author":"H Kavanagh","year":"2010","journal-title":"Clin Exp Allergy"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1067\/mai.2003.171","article-title":"The effect of neonatal BCG vaccination on atopy and asthma at age 7 to 14 years: an historical cohort study in a community with a very low prevalence of tuberculosis infection and a high prevalence of atopic disease.","volume":"111","author":"GB Marks","year":"2003","journal-title":"J Allergy Clin Immunol"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"765","DOI":"10.1128\/CVI.00034-08","article-title":"Enhanced protection against bovine tuberculosis after coadministration of Mycobacterium bovis BCG with a Mycobacterial protein vaccine-adjuvant combination but not after coadministration of adjuvant alone.","volume":"15","author":"DN Wedlock","year":"2008","journal-title":"Clin Vaccine Immunol"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"1339","DOI":"10.1016\/S0140-6736(95)92348-9","article-title":"Variation in protection by BCG: implications of and for heterologous immunity.","volume":"346","author":"PE Fine","year":"1995","journal-title":"Lancet"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"672","DOI":"10.1128\/IAI.70.2.672-678.2002","article-title":"Failure of the Mycobacterium bovis BCG vaccine: some species of environmental mycobacteria block multiplication of BCG and induction of protective immunity to tuberculosis.","volume":"70","author":"L Brandt","year":"2002","journal-title":"Infect Immun"},{"key":"ref45","first-page":"x, 545 p. p","article-title":"Molecular cloning : a laboratory manual.","author":"T Maniatis","year":"1982"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"e6297","DOI":"10.1371\/journal.pone.0006297","article-title":"Bacterial thymidine kinase as a non-invasive imaging reporter for Mycobacterium tuberculosis in live animals.","volume":"4","author":"SL Davis","year":"2009","journal-title":"PLoS One"},{"key":"ref47","first-page":"xii, 363 p. p","article-title":"Molecular genetics of mycobacteria.","author":"GF Hatfull","year":"2000"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.1038\/nprot.2008.73","article-title":"Analyzing real-time PCR data by the comparative C(T) method.","volume":"3","author":"TD Schmittgen","year":"2008","journal-title":"Nat Protoc"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0013773","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,3,31]],"date-time":"2024-03-31T22:13:36Z","timestamp":1711923216000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0013773"}},"subtitle":[],"editor":[{"given":"Katy C.","family":"Kao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2010,10,29]]},"references-count":48,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2010,10,29]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0013773","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,10,29]]}}}