{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T08:54:37Z","timestamp":1777625677923,"version":"3.51.4"},"reference-count":36,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2011,1,21]],"date-time":"2011-01-21T00:00:00Z","timestamp":1295568000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0015572","type":"journal-article","created":{"date-parts":[[2011,1,21]],"date-time":"2011-01-21T21:35:54Z","timestamp":1295645754000},"page":"e15572","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":26,"title":["Surface Localization of Glucosylceramide during Cryptococcus neoformans Infection Allows Targeting as a Potential Antifungal"],"prefix":"10.1371","volume":"6","author":[{"given":"Ryan","family":"Rhome","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arpita","family":"Singh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Talar","family":"Kechichian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monica","family":"Drago","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giulia","family":"Morace","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chiara","family":"Luberto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maurizio Del","family":"Poeta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2011,1,21]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1016\/j.idc.2006.07.001","article-title":"Cryptococcosis.","volume":"20","author":"M Chayakulkeeree","year":"2006","journal-title":"Infect Dis Clin North Am"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"1360","DOI":"10.1038\/nature04220","article-title":"Same-sex mating and the origin of the Vancouver Island Cryptococcus gattii outbreak.","volume":"437","author":"JA Fraser","year":"2005","journal-title":"Nature"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1097\/QAD.0b013e328322ffc3","article-title":"The burden of HIV-associated cryptococcal disease.","volume":"23","author":"TS Harrison","year":"2009","journal-title":"AIDS"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1097\/QAD.0b013e328322ffac","article-title":"Estimation of the current global burden of cryptococcal meningitis among persons living with HIV\/AIDS.","volume":"23","author":"BJ Park","year":"2009","journal-title":"AIDS"},{"key":"ref5","first-page":"271","article-title":"<italic>Cryptococcus neoformans<\/italic>; Press ASfM, editor.","author":"A Casadevall","year":"1998"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"131","DOI":"10.7883\/yoken.JJID.2005.131","article-title":"Sphingolipids in infectious diseases.","volume":"58","author":"K Hanada","year":"2005","journal-title":"Jpn J Infect Dis"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1128\/IAI.74.1.28-39.2006","article-title":"Role of sphingolipids in microbial pathogenesis.","volume":"74","author":"LJ Heung","year":"2006","journal-title":"Infect Immun"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1007\/978-1-4419-6741-1_16","article-title":"Sphingolipid signaling in fungal pathogens.","volume":"688","author":"R Rhome","year":"2010","journal-title":"Adv Exp Med Biol"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1016\/j.mib.2006.06.003","article-title":"Lipid signaling in pathogenic fungi.","volume":"9","author":"JM Shea","year":"2006","journal-title":"Curr Opin Microbiol"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1038\/msb.2008.17","article-title":"Mathematical modeling of pathogenicity of <italic>Cryptococcus neoformans<\/italic>.","volume":"4","author":"J Garcia","year":"2008","journal-title":"Molecular System Biology"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"28547","DOI":"10.1074\/jbc.M503404200","article-title":"The role and mechanism of diacylglycerol-protein kinase C1 signaling in melanogenesis by <italic>Cryptococcus neoformans<\/italic>.","volume":"280","author":"LJ Heung","year":"2005","journal-title":"J Biol Chem"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"21144","DOI":"10.1074\/jbc.M312995200","article-title":"The sphingolipid pathway regulates protein kinase C 1 (Pkc1) through the formation of diacylglycerol (DAG) in <italic>Cryptococcus neoformans<\/italic>.","volume":"279","author":"LJ Heung","year":"2004","journal-title":"J Biol Chem"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"4792","DOI":"10.1128\/IAI.00587-07","article-title":"Depletion of alveolar macrophages decreases the dissemination of a glucosylceramide-deficient mutant of <italic>Cryptococcus neoformans<\/italic> in immunodeficient mice.","volume":"75","author":"TB Kechichian","year":"2007","journal-title":"Infect Immun"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1101\/gad.856001","article-title":"Roles for inositol-phosphoryl ceramide synthase 1 (<italic>IPC1<\/italic>) in pathogenesis of <italic>C. neoformans<\/italic>.","volume":"15","author":"C Luberto","year":"2001","journal-title":"Genes Dev"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"1651","DOI":"10.1172\/JCI27890","article-title":"Glucosylceramide is an essential regulator of pathogenicity of <italic>Cryptococcus neoformans<\/italic>.","volume":"116","author":"PC Rittershaus","year":"2006","journal-title":"J Clin Invest"},{"key":"ref16","article-title":"Identification and characterization of inositol phosphosphingolipid-phospholipase C (<italic>ISC1<\/italic>) from <italic>Cryptococcus neoformans<\/italic>.; 2005 June 5-9.","author":"J Shea"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/S0014-5793(02)03067-3","article-title":"Disruption of the glucosylceramide biosynthetic pathway in Aspergillus nidulans and Aspergillus fumigatus by inhibitors of UDP-Glc:ceramide glucosyltransferase strongly affects spore germination, cell cycle, and hyphal growth.","volume":"525","author":"SB Levery","year":"2002","journal-title":"FEBS Lett"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"1651","DOI":"10.1172\/JCI27890","article-title":"Glucosylceramide synthase is an essential regulator of pathogenicity of Cryptococcus neoformans.","volume":"116","author":"PC Rittershaus","year":"2006","journal-title":"J Clin Invest"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"1715","DOI":"10.1128\/EC.00208-07","article-title":"Biosynthesis and immunogenicity of glucosylceramide in Cryptococcus neoformans and other human pathogens.","volume":"6","author":"R Rhome","year":"2007","journal-title":"Eukaryot Cell"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"919","DOI":"10.1007\/s00018-003-2243-4","article-title":"Recently discovered functions of glucosylceramides in plants and fungi.","volume":"60","author":"D Warnecke","year":"2003","journal-title":"Cell Mol Life Sci"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1007\/s00253-005-0187-3","article-title":"Presence of glucosylceramide in yeast and its relation to alkali tolerance of yeast.","volume":"71","author":"K Saito","year":"2006","journal-title":"Appl Microbiol Biotechnol"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"7049","DOI":"10.1128\/IAI.68.12.7049-7060.2000","article-title":"Human antibodies against a purified glucosylceramide from Cryptococcus neoformans inhibit cell budding and fungal growth.","volume":"68","author":"ML Rodrigues","year":"2000","journal-title":"Infect Immun"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1128\/EC.00318-06","article-title":"Vesicular polysaccharide export in Cryptococcus neoformans is a eukaryotic solution to the problem of fungal trans-cell wall transport.","volume":"6","author":"ML Rodrigues","year":"2007","journal-title":"Eukaryot Cell"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1128\/EC.00370-07","article-title":"Extracellular vesicles produced by Cryptococcus neoformans contain protein components associated with virulence.","volume":"7","author":"ML Rodrigues","year":"2008","journal-title":"Eukaryot Cell"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1093\/glycob\/11.2.105","article-title":"Characterization of monoclonal antibody MEST-2 specific to glucosylceramide of fungi and plants.","volume":"11","author":"MS Toledo","year":"2001","journal-title":"Glycobiology"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"1372","DOI":"10.1128\/CVI.00202-07","article-title":"Monoclonal antibody to fungal glucosylceramide protects mice against lethal Cryptococcus neoformans infection.","volume":"14","author":"ML Rodrigues","year":"2007","journal-title":"Clin Vaccine Immunol"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1016\/S0014-5793(03)01058-5","article-title":"Formation of glucosylceramide and sterol glucoside by a UDP-glucose-dependent glucosylceramide synthase from cotton expressed in Pichia pastoris.","volume":"553","author":"I Hillig","year":"2003","journal-title":"FEBS Lett"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"919","DOI":"10.1007\/s00018-003-2243-4","article-title":"Recently discovered functions of glucosylceramides in plants and fungi.","volume":"60","author":"D Warnecke","year":"2003","journal-title":"Cell Mol Life Sci"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"570","DOI":"10.1128\/CDLI.3.5.570-574.1996","article-title":"Extracellular proteinase activity of Cryptococcus neoformans.","volume":"3","author":"LC Chen","year":"1996","journal-title":"Clin Diagn Lab Immunol"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"1234","DOI":"10.1099\/mic.0.033985-0","article-title":"Candida albicans sphingolipid C9-methyltransferase is involved in hyphal elongation.","volume":"156","author":"T Oura","year":"2010","journal-title":"Microbiology"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1093\/glycob\/11.2.105","article-title":"Characterization of monoclonal antibody MEST-2 specific to glucosylceramide of fungi and plants.","volume":"11","author":"MS Toledo","year":"2001","journal-title":"Glycobiology"},{"key":"ref32","article-title":"Antibodies. A laboratory Manual.","volume":"274","author":"E Harlow","year":"1988","journal-title":"Cold Spring Harbor, page"},{"key":"ref33","first-page":"2342","article-title":"Role of host sphingosine kinase 1 in the lung response against Cryptococcosis.","author":"T McQuiston","journal-title":"Infect Immun"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"349","DOI":"10.7164\/antibiotics.48.349","article-title":"The discovery of australifungin, a novel inhibitor of sphinganine N-acyltransferase from Sporormiella australis. Producing organism, fermentation, isolation, and biological activity.","volume":"48","author":"SM Mandala","year":"1995","journal-title":"J Antibiot (Tokyo)"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"2421","DOI":"10.1128\/AEM.63.6.2421-2431.1997","article-title":"Bacterial viability and antibiotic susceptibility testing with SYTOX green nucleic acid stain.","volume":"63","author":"BL Roth","year":"1997","journal-title":"Appl Environ Microbiol"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"5451","DOI":"10.1128\/AEM.65.12.5451-5458.1999","article-title":"Permeabilization of fungal membranes by plant defensins inhibits fungal growth.","volume":"65","author":"K Thevissen","year":"1999","journal-title":"Appl Environ Microbiol"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0015572","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,4,3]],"date-time":"2024-04-03T10:08:10Z","timestamp":1712138890000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0015572"}},"subtitle":[],"editor":[{"given":"Francoise","family":"Dromer","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2011,1,21]]},"references-count":36,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2011,1,21]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0015572","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,1,21]]}}}