{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T08:48:07Z","timestamp":1790930887509,"version":"4.1.0"},"reference-count":56,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2010,12,22]],"date-time":"2010-12-22T00:00:00Z","timestamp":1292976000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0015667","type":"journal-article","created":{"date-parts":[[2010,12,22]],"date-time":"2010-12-22T23:43:45Z","timestamp":1293061425000},"page":"e15667","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":77,"title":["Simultaneous Analysis of Multiple Mycobacterium tuberculosis Knockdown Mutants In Vitro and In Vivo"],"prefix":"10.1371","volume":"5","author":[{"given":"Antje","family":"Blumenthal","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolina","family":"Trujillo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabine","family":"Ehrt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dirk","family":"Schnappinger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2010,12,22]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"856","DOI":"10.1126\/science.1185449","article-title":"The population dynamics and control of tuberculosis.","volume":"328","author":"C Dye","year":"2010","journal-title":"Science"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1097\/QCO.0b013e3283229fab","article-title":"Extensively drug-resistant tuberculosis.","volume":"22","author":"P LoBue","year":"2009","journal-title":"Curr Opin Infect Dis"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"1075","DOI":"10.1164\/rccm.200801-132OC","article-title":"Treatment outcomes and long-term survival in patients with extensively drug-resistant tuberculosis.","volume":"178","author":"DH Kim","year":"2008","journal-title":"Am J Respir Crit Care Med"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"1575","DOI":"10.1016\/S0140-6736(06)69573-1","article-title":"Extensively drug-resistant tuberculosis as a cause of death in patients co-infected with tuberculosis and HIV in a rural area of South Africa.","volume":"368","author":"NR Gandhi","year":"2006","journal-title":"Lancet"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"e21","DOI":"10.1093\/nar\/gni013","article-title":"Controlling gene expression in mycobacteria with anhydrotetracycline and Tet repressor.","volume":"33","author":"S Ehrt","year":"2005","journal-title":"Nucleic Acids Res"},{"key":"ref6","article-title":"Improved tetracycline repressors for gene silencing in mycobacteria.","author":"M Klotzsche","year":"2009","journal-title":"Nucleic Acids Res"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"e22","DOI":"10.1093\/nar\/gni023","article-title":"Tetracycline-inducible gene regulation in mycobacteria.","volume":"33","author":"MC Blokpoel","year":"2005","journal-title":"Nucleic Acids Res"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"3077","DOI":"10.1128\/AEM.71.6.3077-3084.2005","article-title":"Use of a tetracycline-inducible system for conditional expression in Mycobacterium tuberculosis and Mycobacterium smegmatis.","volume":"71","author":"P Carroll","year":"2005","journal-title":"Appl Environ Microbiol"},{"key":"ref9","article-title":"Development of a repressible mycobacterial promoter system based on two transcriptional repressors.","author":"F Boldrin","year":"2010","journal-title":"Nucleic Acids Res"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.tube.2008.07.007","article-title":"Nitrile-inducible gene expression in mycobacteria.","volume":"89","author":"AK Pandey","year":"2009","journal-title":"Tuberculosis (Edinb)"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1016\/j.jbiotec.2009.02.001","article-title":"Pristinamycin-inducible gene regulation in mycobacteria.","volume":"140","author":"F Forti","year":"2009","journal-title":"J Biotechnol"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.plasmid.2010.04.003","article-title":"Improved mycobacterial tetracycline inducible vectors.","volume":"64","author":"KJ Williams","year":"2010","journal-title":"Plasmid"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1111\/j.1574-6968.2007.00842.x","article-title":"Expression of Mycobacterium tuberculosis Rv1991c using an arabinose-inducible promoter demonstrates its role as a toxin.","volume":"274","author":"P Carroll","year":"2007","journal-title":"FEMS Microbiol Lett"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1007\/s00203-006-0160-2","article-title":"Tetracycline-inducible gene expression in mycobacteria within an animal host using modified Streptomyces tcp830 regulatory elements.","volume":"186","author":"SM Hernandez-Abanto","year":"2006","journal-title":"Arch Microbiol"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"e1000957","DOI":"10.1371\/journal.ppat.1000957","article-title":"EspA acts as a critical mediator of ESX1-dependent virulence in Mycobacterium tuberculosis by affecting bacterial cell wall integrity.","volume":"6","author":"A Garces","year":"2010","journal-title":"PLoS Pathog"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"18792","DOI":"10.1073\/pnas.0900589106","article-title":"Mycobacterial Esx-3 is required for mycobactin-mediated iron acquisition.","volume":"106","author":"MS Siegrist","year":"2009","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref17","article-title":"Characterization of a Mycobacterium tuberculosis ESX-3 conditional mutant: essentiality and rescue by Iron and Zinc.","author":"A Serafini","year":"2009","journal-title":"J Bacteriol"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"4614","DOI":"10.1128\/JB.00216-07","article-title":"Silencing essential protein secretion in Mycobacterium smegmatis by using tetracycline repressors.","volume":"189","author":"XV Guo","year":"2007","journal-title":"J Bacteriol"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"9927","DOI":"10.1074\/jbc.M900325200","article-title":"The Accessory SecA2 System of Mycobacteria Requires ATP Binding and the Canonical SecA1.","volume":"284","author":"NW Rigel","year":"2009","journal-title":"J Biol Chem"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"717","DOI":"10.1038\/nature07219","article-title":"Secreted transcription factor controls Mycobacterium tuberculosis virulence.","volume":"454","author":"S Raghavan","year":"2008","journal-title":"Nature"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1073\/pnas.0912952107","article-title":"Inhibition of bacterial disulfide bond formation by the anticoagulant warfarin.","volume":"107","author":"RJ Dutton","year":"2010","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"5237","DOI":"10.1128\/JB.00332-07","article-title":"Mycobacterial nonhomologous end joining mediates mutagenic repair of chromosomal double-strand DNA breaks.","volume":"189","author":"NC Stephanou","year":"2007","journal-title":"J Bacteriol"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"15114","DOI":"10.1074\/jbc.M701167200","article-title":"Mycobacterial UvrD1 is a Ku-dependent DNA helicase that plays a role in multiple DNA repair events, including double-strand break repair.","volume":"282","author":"KM Sinha","year":"2007","journal-title":"J Biol Chem"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"e1000001","DOI":"10.1371\/journal.ppat.1000001","article-title":"A mycobacterial enzyme essential for cell division synergizes with resuscitation-promoting factor.","volume":"4","author":"EC Hett","year":"2008","journal-title":"PLoS Pathog"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"658","DOI":"10.1111\/j.1365-2958.2007.05945.x","article-title":"A partner for the resuscitation-promoting factors of Mycobacterium tuberculosis.","volume":"66","author":"EC Hett","year":"2007","journal-title":"Mol Microbiol"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"961","DOI":"10.1099\/00221287-148-4-961","article-title":"Physiological consequences associated with overproduction of Mycobacterium tuberculosis FtsZ in mycobacterial hosts.","volume":"148","author":"J Dziadek","year":"2002","journal-title":"Microbiology"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"e1001040","DOI":"10.1371\/journal.ppat.1001040","article-title":"The <italic>Mycobacterium tuberculosis<\/italic> Proteasome Active Site Threonine Is Essential for Persistence Yet Dispensable for Replication and Resistance to Nitric Oxide.","volume":"6","author":"S Gandotra","year":"2010","journal-title":"PLoS Pathog"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"8511","DOI":"10.1073\/pnas.0511129103","article-title":"The nonredundant roles of two 4&apos;-phosphopantetheinyl transferases in vital processes of Mycobacteria.","volume":"103","author":"C Chalut","year":"2006","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1099\/mic.0.2007\/014076-0","article-title":"Wag31, a homologue of the cell division protein DivIVA, regulates growth, morphology and polar cell wall synthesis in mycobacteria.","volume":"154","author":"CM Kang","year":"2008","journal-title":"Microbiology"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1111\/j.1365-2958.2009.06750.x","article-title":"Novel role of Wag31 in protection of mycobacteria under oxidative stress.","volume":"73","author":"P Mukherjee","year":"2009","journal-title":"Mol Microbiol"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"3887","DOI":"10.1099\/00221287-148-12-3887","article-title":"Conditional expression of Mycobacterium smegmatis dnaA, an essential DNA replication gene.","volume":"148","author":"R Greendyke","year":"2002","journal-title":"Microbiology"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"9819","DOI":"10.1073\/pnas.1000715107","article-title":"Gluconeogenic carbon flow of tricarboxylic acid cycle intermediates is critical for Mycobacterium tuberculosis to establish and maintain infection.","volume":"107","author":"J Marrero","year":"2010","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1016\/j.cell.2009.04.041","article-title":"CarD is an essential regulator of rRNA transcription required for Mycobacterium tuberculosis persistence.","volume":"138","author":"CL Stallings","year":"2009","journal-title":"Cell"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1111\/j.1365-2958.2007.05814.x","article-title":"Polyphosphate kinase is involved in stress-induced mprAB-sigE-rel signalling in mycobacteria.","volume":"65","author":"K Sureka","year":"2007","journal-title":"Mol Microbiol"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"2111","DOI":"10.1101\/gr.2512204","article-title":"Concerted assembly and cloning of multiple DNA segments using in vitro site-specific recombination: functional analysis of multi-segment expression clones.","volume":"14","author":"DL Cheo","year":"2004","journal-title":"Genome Res"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"3111","DOI":"10.1073\/pnas.88.8.3111","article-title":"Site-specific integration of mycobacteriophage L5: integration-proficient vectors for Mycobacterium smegmatis, Mycobacterium tuberculosis, and bacille Calmette-Guerin.","volume":"88","author":"MH Lee","year":"1991","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1111\/j.1462-5822.2005.00648.x","article-title":"Carbon metabolism of intracellular bacteria.","volume":"8","author":"EJ Munoz-Elias","year":"2006","journal-title":"Cell Microbiol"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"940","DOI":"10.1111\/j.1365-2958.2006.05297.x","article-title":"Dual role of isocitrate lyase 1 in the glyoxylate and methylcitrate cycles in Mycobacterium tuberculosis.","volume":"61","author":"TA Gould","year":"2006","journal-title":"Mol Microbiol"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"1109","DOI":"10.1111\/j.1365-2958.2006.05155.x","article-title":"Role of the methylcitrate cycle in Mycobacterium tuberculosis metabolism, intracellular growth, and virulence.","volume":"60","author":"EJ Munoz-Elias","year":"2006","journal-title":"Mol Microbiol"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"638","DOI":"10.1038\/nm1252","article-title":"Mycobacterium tuberculosis isocitrate lyases 1 and 2 are jointly required for in vivo growth and virulence.","volume":"11","author":"EJ Munoz-Elias","year":"2005","journal-title":"Nat Med"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"849","DOI":"10.1038\/nm.1795","article-title":"A membrane protein preserves intrabacterial pH in intraphagosomal Mycobacterium tuberculosis.","volume":"14","author":"OH Vandal","year":"2008","journal-title":"Nat Med"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"1150","DOI":"10.1016\/j.micinf.2009.08.003","article-title":"The Mycobacterium tuberculosis proteasome: more than just a barrel-shaped protease.","volume":"11","author":"F Cerda-Maira","year":"2009","journal-title":"Microbes Infect"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"1515","DOI":"10.1038\/nm1683","article-title":"In vivo gene silencing identifies the Mycobacterium tuberculosis proteasome as essential for the bacteria to persist in mice.","volume":"13","author":"S Gandotra","year":"2007","journal-title":"Nat Med"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"735","DOI":"10.1038\/35021074","article-title":"Persistence of Mycobacterium tuberculosis in macrophages and mice requires the glyoxylate shunt enzyme isocitrate lyase.","volume":"406","author":"JD McKinney","year":"2000","journal-title":"Nature"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"1473","DOI":"10.1101\/gr.191301","article-title":"A novel method of gene transcript profiling in airway biopsy homogenates reveals increased expression of a Na+-K+-Cl- cotransporter (NKCC1) in asthmatic subjects.","volume":"11","author":"GM Dolganov","year":"2001","journal-title":"Genome Res"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"12712","DOI":"10.1073\/pnas.231275498","article-title":"Comprehensive identification of conditionally essential genes in mycobacteria.","volume":"98","author":"CM Sassetti","year":"2001","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1046\/j.1365-2958.2003.03425.x","article-title":"Genes required for mycobacterial growth defined by high density mutagenesis.","volume":"48","author":"CM Sassetti","year":"2003","journal-title":"Mol Microbiol"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"12989","DOI":"10.1073\/pnas.2134250100","article-title":"Genetic requirements for mycobacterial survival during infection.","volume":"100","author":"CM Sassetti","year":"2003","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"929","DOI":"10.1038\/nrg1984","article-title":"Signature-tagged mutagenesis: barcoding mutants for genome-wide screens.","volume":"7","author":"P Mazurkiewicz","year":"2006","journal-title":"Nat Rev Genet"},{"key":"ref50","doi-asserted-by":"crossref","first-page":"8327","DOI":"10.1073\/pnas.0503272102","article-title":"Genome-wide requirements for Mycobacterium tuberculosis adaptation and survival in macrophages.","volume":"102","author":"J Rengarajan","year":"2005","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1038\/47042","article-title":"Complex lipid determines tissue-specific replication of Mycobacterium tuberculosis in mice.","volume":"402","author":"JS Cox","year":"1999","journal-title":"Nature"},{"key":"ref52","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1046\/j.1365-2958.1999.01593.x","article-title":"Identification of a virulence gene cluster of Mycobacterium tuberculosis by signature-tagged transposon mutagenesis.","volume":"34","author":"LR Camacho","year":"1999","journal-title":"Mol Microbiol"},{"key":"ref53","doi-asserted-by":"crossref","first-page":"5315","DOI":"10.1128\/IAI.72.9.5315-5321.2004","article-title":"Identification of Mycobacterium tuberculosis counterimmune (cim) mutants in immunodeficient mice by differential screening.","volume":"72","author":"KB Hisert","year":"2004","journal-title":"Infect Immun"},{"key":"ref54","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1001100","article-title":"High content phenotypic cell-based visual screen identifies Mycobacterium tuberculosis acyltrehalose-containing glycolipids involved in phagosome remodeling.","volume":"6","author":"P Brodin","year":"2010","journal-title":"PLoS Pathog"},{"key":"ref55","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1128\/IAI.00058-06","article-title":"Signature-tagged transposon mutagenesis identifies novel Mycobacterium tuberculosis genes involved in the parasitism of human macrophages.","volume":"75","author":"V Rosas-Magallanes","year":"2007","journal-title":"Infect Immun"},{"key":"ref56","doi-asserted-by":"crossref","first-page":"2578","DOI":"10.1128\/JCM.29.11.2578-2586.1991","article-title":"Occurrence and stability of insertion sequences in Mycobacterium tuberculosis complex strains: evaluation of an insertion sequence-dependent DNA polymorphism as a tool in the epidemiology of tuberculosis.","volume":"29","author":"D van Soolingen","year":"1991","journal-title":"J Clin Microbiol"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0015667","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,6,14]],"date-time":"2020-06-14T23:21:54Z","timestamp":1592176914000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0015667"}},"subtitle":[],"editor":[{"given":"Ludovic","family":"Tailleux","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2010,12,22]]},"references-count":56,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2010,12,22]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0015667","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,12,22]]}}}