{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T11:25:41Z","timestamp":1789385141608,"version":"build-2803163510"},"reference-count":49,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2011,2,23]],"date-time":"2011-02-23T00:00:00Z","timestamp":1298419200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0017383","type":"journal-article","created":{"date-parts":[[2011,2,24]],"date-time":"2011-02-24T01:22:45Z","timestamp":1298510565000},"page":"e17383","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":14,"title":["Adenylate Cyclase Toxin Promotes Internalisation of Integrins and Raft Components and Decreases Macrophage Adhesion Capacity"],"prefix":"10.1371","volume":"6","author":[{"given":"C\u00e9sar","family":"Mart\u00edn","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kepa B.","family":"Uribe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geraxane","family":"G\u00f3mez-Bilbao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helena","family":"Ostolaza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2011,2,23]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"3445","DOI":"10.1128\/IAI.58.10.3445-3447.1990","article-title":"Adenylate cyclase toxin is critical for colonization and pertussis toxin is critical for lethal infection by Bordetella pertussis in infant mice.","volume":"58","author":"MS Goodwin","year":"1990","journal-title":"Infect Immun"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"E168","DOI":"10.2741\/Mattoo","article-title":"Mechanisms of Bordetella pathogenesis.","volume":"6","author":"S Mattoo","year":"2001","journal-title":"Front Biosci"},{"key":"ref3","first-page":"356","article-title":"Activation of prokaryotic adenylate cyclase by calmodulin.","author":"SA Berkowitz","year":"1980","journal-title":"Ann N Y Acad Sci"},{"issue":"34","key":"ref4","doi-asserted-by":"crossref","first-page":"16264","DOI":"10.1016\/S0021-9258(18)66710-9","article-title":"Bordetella pertussis adenylate cyclase. Purification, characterization, and radioimmunoassay.","volume":"261","author":"D Ladant","year":"1986","journal-title":"J Biol Chem"},{"issue":"32","key":"ref5","doi-asserted-by":"crossref","first-page":"19379","DOI":"10.1016\/S0021-9258(19)47312-2","article-title":"Adenylate cyclase toxin from Bordetella pertussis. Identification and purification of the holotoxin molecule.","volume":"264","author":"EL Hewlett","year":"1989","journal-title":"J Biol Chem"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"2698","DOI":"10.1128\/IAI.73.5.2698-2703.2005","article-title":"Pertussis toxin and adenylate cyclase toxin provide a one-two punch for establishment of Bordetella pertussis infection of the respiratory tract.","volume":"73","author":"NH Carbonetti","year":"2005","journal-title":"Infect Immun"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"1035","DOI":"10.1084\/jem.193.9.1035","article-title":"The adenylate cyclase toxin of Bordetella pertussis binds to target cells via the alpha(M)beta(2) integrin (CD11b\/CD18).","volume":"193","author":"P Guermonprez","year":"2001","journal-title":"Exp Med"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"3154","DOI":"10.1016\/S0021-9258(18)49967-X","article-title":"Adenylate cyclase toxin from Bordetella pertussis. The relationship between induction of cAMP and hemolysis.","volume":"266","author":"A Rogel","year":"1991","journal-title":"J Biol Chem"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1016\/S0966-842X(99)01468-7","article-title":"Bordatella pertussis adenylate cyclase: a toxin with multiple talents.","volume":"7","author":"D Ladant","year":"1999","journal-title":"Trends Microbiol"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"27231","DOI":"10.1016\/S0021-9258(18)46973-6","article-title":"Adenylate cyclase toxin (CyaA) of Bordetella pertussis. Evidence for the formation of small ion-permeable channels and comparison with HlyA of Escherichia coli.","volume":"269","author":"R Benz","year":"1994","journal-title":"J Biol Chem"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"3760","DOI":"10.1128\/JB.186.12.3760-3765.2004","article-title":"Membrane restructuring by Bordetella pertussis adenylate cyclase toxin, a member of the RTX toxin family.","volume":"186","author":"C Mart\u00edn","year":"2004","journal-title":"J Bacteriol"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"2808","DOI":"10.1074\/jbc.M609979200","article-title":"Third activity of Bordetella adenylate cyclase (AC) toxin-hemolysin. Membrane translocation of AC domain polypeptide promotes calcium influx into CD11b+ monocytes independently of the catalytic and hemolytic activities.","volume":"282","author":"R Fiser","year":"2007","journal-title":"J Biol Chem"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1074\/jbc.M109.003491","article-title":"Bordetella adenylate cyclase toxin promotes calcium entry into both CD11b+ and CD11b- cells through cAMP-dependent L-type-like calcium channels.","volume":"285","author":"C Mart\u00edn","year":"2010","journal-title":"J Biol Chem"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"47792","DOI":"10.1074\/jbc.M406824200","article-title":"Rafts can trigger contact-mediated secretion of bacterial effectors via a lipid-based mechanism.","volume":"279","author":"FG van der Goot","year":"2004","journal-title":"J Biol Chem"},{"key":"ref15","first-page":"85","article-title":"RTX toxin structure and function: a story of numerous anomalies and few analogies in toxin biology.","volume":"257","author":"RA Welch","year":"2001","journal-title":"Curr Top Microbiol Immunol"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1126\/science.7939682","article-title":"Internal lysine palmitoylation in adenylate cyclase toxin from Bordetella pertussis.","volume":"266","author":"M Hackett","year":"1994","journal-title":"Science"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"3910","DOI":"10.1074\/jbc.274.6.3910","article-title":"Role of lipid modifications in targeting proteins to detergent-resistant membrane rafts. Many raft proteins are acylated, while few are prenylated.","volume":"274","author":"KA Melkonian","year":"1999","journal-title":"J Biol Chem"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"1753","DOI":"10.1111\/j.1462-5822.2006.00746.x","article-title":"Actinobacillus actinomycetemcomitans leukotoxin requires lipid microdomains for target cell cytotoxicity.","volume":"8","author":"KP Fong","year":"2006","journal-title":"Cell Microbiol"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"4719","DOI":"10.1128\/IAI.00534-07","article-title":"Mannheimia haemolytica leukotoxin binds to lipid rafts in bovine lymphoblastoid cells and is internalized in a dynamin-2- and clathrin-dependent manner.","volume":"75","author":"DN Atapattu","year":"2007","journal-title":"Infect Immun"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1038\/42408","article-title":"Functional rafts in cell membranes.","volume":"387","author":"K Simons","year":"1997","journal-title":"Nature"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1006\/bbrc.1997.7575","article-title":"Structure of detergent-resistant membrane domains: does phase separation occur in biological membranes?","volume":"240","author":"DA Brown","year":"1997","journal-title":"Biochem Biophys Res Commun"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1111\/j.1600-0854.2004.0181.x","article-title":"Lipids as targeting signals: lipid rafts and intracellular trafficking.","volume":"5","author":"JB Helms","year":"2004","journal-title":"Traffic"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"2721","DOI":"10.1083\/jcb.109.6.2721","article-title":"Endocytosis of simian virus 40 into the endoplasmic reticulum.","volume":"109","author":"J Kartenbeck","year":"1989","journal-title":"J Cell Biol"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1016\/S0962-8924(99)01567-6","article-title":"Landing on lipid rafts.","volume":"9","author":"M Fivaz","year":"1999","journal-title":"Trends Cell Biol"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"613","DOI":"10.1111\/j.1462-5822.2005.00515.x","article-title":"Bacterial invasion via lipid rafts.","volume":"7","author":"F Lafont","year":"2005","journal-title":"Cell Microbiol"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"17221","DOI":"10.1074\/jbc.R000005200","article-title":"Structure and Function of Sphingolipid- and Cholesterol-rich Membrane Rafts","volume":"275","author":"DA Brown","year":"2000","journal-title":"J Biol Chem"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"556","DOI":"10.1002\/(SICI)1521-4141(199902)29:02<556::AID-IMMU556>3.0.CO;2-2","article-title":"Clusters of glycolipid and glycosylphosphatidylinositol-anchored proteins in lymphoid cells: accumulation of actin regulated by local tyrosine phosphorylation.","volume":"29","author":"T Harder","year":"1999","journal-title":"Eur J Immunol"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"963","DOI":"10.1242\/jcs.115.5.963","article-title":"The involvement of lipid rafts in the regulation of integrin function.","volume":"115","author":"B Leitinger","year":"2002","journal-title":"J Cell Sci"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"699","DOI":"10.1083\/jcb.140.3.699","article-title":"LFA-1-mediated adhesion is regulated by cytoskeletal restraint and by a Ca<sup>2+<\/sup>-dependent protease, calpain.","volume":"140","author":"MP Stewart","year":"1998","journal-title":"J Cell Biol"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"e1000901","DOI":"10.1371\/journal.ppat.1000901","article-title":"Bordetella adenylate cyclase toxin mobilizes its beta2 integrin receptor into lipid rafts to accomplish translocation across target cell membrane in two steps.","volume":"6","author":"L Bumba","year":"2010","journal-title":"PLoS Pathog"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1038\/nri1129","article-title":"Pathogens: raft hijackers.","volume":"3","author":"S Ma\u00f1es","year":"2003","journal-title":"Nat Rev Immunol"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1016\/j.chom.2007.10.001","article-title":"Invasive and adherent bacterial pathogens co-Opt host clathrin for infection.","volume":"2","author":"E Veiga","year":"2007","journal-title":"Cell Host Microbe"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1002\/j.1460-2075.1992.tb05066.x","article-title":"Cells can use their transferrin receptors for locomotion.","volume":"11","author":"MS Bretscher","year":"1992","journal-title":"EMBO J"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"191","DOI":"10.4049\/jimmunol.144.1.191","article-title":"Constitutive and induced phosphorylation of the alpha- and beta-chains of the CD11\/CD18 leukocyte integrin family. Relationship to adhesion-dependent functions.","volume":"144","author":"JP Buyon","year":"1990","journal-title":"J Immunol"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"26164","DOI":"10.1074\/jbc.M011291200","article-title":"Caveolin-1 and caveolin-2 expression in mouse macrophages. High density lipoprotein 3-stimulated secretion and a lack of significant subcellular co-localization.","volume":"276","author":"P Gargalovic","year":"2001","journal-title":"J Biol Chem"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"1037","DOI":"10.1182\/blood.V75.5.1037.1037","article-title":"Structure and function of the leukocyte adhesion molecules CD11\/CD18.","volume":"75","author":"MA Arnaout","year":"1990","journal-title":"Blood"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"694","DOI":"10.1038\/35015091","article-title":"Alpha-haemolysin of uropathogenic E. coli induces Ca<sup>2+<\/sup> oscillations in renal epithelial cells.","volume":"405","author":"P Uhl\u00e9n","year":"2000","journal-title":"Nature"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"973","DOI":"10.1096\/fj.05-4561fje","article-title":"Why Escherichia coli alpha-hemolysin induces calcium oscillations in mammalian cells: the pore is on its own.","volume":"20","author":"A Koschinski","year":"2006","journal-title":"FASEB J"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"721","DOI":"10.1046\/j.1462-5822.2001.00151.x","article-title":"Internalization of Bordetella pertussis adenylate cyclase-haemolysin into endocytic vesicles contributes to macrophage cytotoxicity.","volume":"3","author":"N Khelef","year":"2001","journal-title":"Cell Microbiol"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1002\/jemt.20277","article-title":"Bordetella adenylate cyclase toxin induces a cascade of morphological changes of sheep erythrocytes and localizes into clusters in erythrocyte membranes.","volume":"69","author":"J Vojtov\u00e1","year":"2006","journal-title":"Microsc Res Tech"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1007\/978-90-481-8622-8_19","article-title":"Cholesterol and ion channels.","volume":"51","author":"I Levitan","year":"2010","journal-title":"Subcell Biochem"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"496","DOI":"10.1002\/bies.200900172","article-title":"Clathrin-mediated endocytosis: what works for small, also works for big.","volume":"32","author":"J Pizarro-Cerd\u00e1","year":"2010","journal-title":"Bioessays"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"10662","DOI":"10.1074\/jbc.M109.089219","article-title":"Selective translocation of the Bordetella pertussis adenylate cyclase toxin across the basolateral membranes of polarized epithelial cells.","volume":"285","author":"JC Eby","year":"2010","journal-title":"J Biol Chem"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"3759","DOI":"10.1128\/IAI.58.11.3759-3764.1990","article-title":"Bordetella pertussis adenylate cyclase toxin: intoxication of host cells by bacterial invasion.","volume":"58","author":"M Mouallem","year":"1990","journal-title":"Infect Immun"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"2822","DOI":"10.1128\/IAI.00200-08","article-title":"Suppression of T-lymphocyte activation and chemotaxis by the adenylate cyclase toxin of Bordetella pertussis.","volume":"76","author":"SR Paccani","year":"2008","journal-title":"Infect Immun"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"470","DOI":"10.1016\/j.coi.2007.05.002","article-title":"Plasma membrane segregation during T cell activation: probing the order of domains.","volume":"19","author":"T Harder","year":"2007","journal-title":"Curr Opin Immunol"},{"issue":"Pt 11","key":"ref47","doi-asserted-by":"crossref","first-page":"1773","DOI":"10.1242\/jcs.018036","article-title":"Integrin-dependent phagocytosis: spreading from microadhesion to new concepts.","volume":"121","author":"AG Dupuy","year":"2008","journal-title":"J Cell Sci"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1016\/j.molimm.2005.04.011","article-title":"The role of lipid rafts in LPS-induced signaling in a macrophage cell line.","volume":"43","author":"S Olsson","year":"2006","journal-title":"Mol Immunol"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"3440","DOI":"10.1016\/S0021-9258(19)83641-4","article-title":"A new generation of Ca<sup>2+<\/sup> indicators with greatly improved fluorescence properties.","volume":"260","author":"G Grynkiewicz","year":"1985","journal-title":"J Biol Chem"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0017383","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,6,5]],"date-time":"2023-06-05T10:57:51Z","timestamp":1685962671000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0017383"}},"subtitle":[],"editor":[{"given":"J\u00e9r\u00f4me","family":"Nigou","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2011,2,23]]},"references-count":49,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2011,2,23]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0017383","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,2,23]]}}}