{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T11:13:22Z","timestamp":1788952402147,"version":"build-2803163510"},"reference-count":48,"publisher":"Public Library of Science (PLoS)","issue":"8","license":[{"start":{"date-parts":[[2011,8,17]],"date-time":"2011-08-17T00:00:00Z","timestamp":1313539200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0023596","type":"journal-article","created":{"date-parts":[[2011,8,17]],"date-time":"2011-08-17T19:39:54Z","timestamp":1313609994000},"page":"e23596","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":33,"title":["Fluorescence Dequenching Makes Haem-Free Soluble Guanylate Cyclase Detectable in Living Cells"],"prefix":"10.1371","volume":"6","author":[{"given":"Linda S.","family":"Hoffmann","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter M.","family":"Schmidt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yvonne","family":"Keim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carsten","family":"Hoffmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harald H. H. W.","family":"Schmidt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johannes-Peter","family":"Stasch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2011,8,17]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"2592","DOI":"10.1073\/pnas.91.7.2592","article-title":"Mutation of His-105 in the beta 1 subunit yields a nitric oxide-insensitive form of soluble guanylyl cyclase.","volume":"91","author":"B Wedel","year":"1994","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"578","DOI":"10.1038\/sj.emboj.7601521","article-title":"NO and CO differentially activate soluble guanylyl cyclase via a heme pivot-bend mechanism.","volume":"26","author":"X Ma","year":"2007","journal-title":"EMBO J"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1016\/S0031-6997(24)01457-1","article-title":"Guanylyl cyclases and signaling by cyclic GMP.","volume":"52","author":"KA Lucas","year":"2000","journal-title":"Pharmacol Rev"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"907","DOI":"10.1161\/01.RES.0000100390.68771.CC","article-title":"Cyclic GMP-dependent protein kinases and the cardiovascular system: insights from genetically modified mice.","volume":"93","author":"R Feil","year":"2003","journal-title":"Circ Res"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"755","DOI":"10.1038\/nrd2038","article-title":"NO-independent stimulators and activators of soluble guanylate cyclase: discovery and therapeutic potential.","volume":"5","author":"OV Evgenov","year":"2006","journal-title":"Nat Rev Drug Discov"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"2330","DOI":"10.1172\/JCI29807","article-title":"Deconstructing endothelial dysfunction: soluble guanylyl cyclase oxidation and the NO resistance syndrome.","volume":"116","author":"MT Gladwin","year":"2006","journal-title":"J Clin Invest"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1016\/j.pharmthera.2007.06.012","article-title":"Impaired tissue responsiveness to organic nitrates and nitric oxide: a new therapeutic frontier?","volume":"116","author":"YY Chirkov","year":"2007","journal-title":"Pharmacol Ther"},{"key":"ref8","article-title":"Oxidation and Loss of Heme in Soluble Guanylyl Cyclase from Manduca Sexta.","author":"BG Fritz","year":"2011","journal-title":"Biochemistry"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"3025","DOI":"10.1074\/jbc.M310141200","article-title":"Identification of residues crucially involved in the binding of the heme moiety of soluble guanylate cyclase.","volume":"279","author":"PM Schmidt","year":"2004","journal-title":"J Biol Chem"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"1495","DOI":"10.1038\/sj.bjp.0707687","article-title":"Probing the presence of the ligand-binding haem in cellular nitric oxide receptors.","volume":"153","author":"B Roy","year":"2008","journal-title":"Br J Pharmacol"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1007\/978-3-540-68964-5_14","article-title":"NO- and haem-independent soluble guanylate cyclase activators.","volume":"191","author":"HH Schmidt","year":"2009","journal-title":"Handb Exp Pharmacol"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1007\/978-3-540-68964-5_13","article-title":"NO-independent, haem-dependent soluble guanylate cyclase stimulators.","volume":"191","author":"JP Stasch","year":"2009","journal-title":"Handb Exp Pharmacol"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"2552","DOI":"10.1172\/JCI28371","article-title":"Targeting the heme-oxidized nitric oxide receptor for selective vasodilatation of diseased blood vessels.","volume":"116","author":"JP Stasch","year":"2006","journal-title":"J Clin Invest"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"773","DOI":"10.1038\/sj.bjp.0704778","article-title":"NO- and haem-independent activation of soluble guanylyl cyclase: molecular basis and cardiovascular implications of a new pharmacological principle.","volume":"136","author":"JP Stasch","year":"2002","journal-title":"Br J Pharmacol"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"1128","DOI":"10.1161\/HYPERTENSIONAHA.106.083832","article-title":"Targeting heme-oxidized soluble guanylate cyclase in experimental heart failure.","volume":"49","author":"G Boerrigter","year":"2007","journal-title":"Hypertension"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1161\/CIRCRESAHA.109.198234","article-title":"Nitric oxide-independent vasodilator rescues heme-oxidized soluble guanylate cyclase from proteasomal degradation.","volume":"105","author":"S Meurer","year":"2009","journal-title":"Circ Res"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"781","DOI":"10.1111\/j.1476-5381.2009.00263.x","article-title":"Distinct molecular requirements for activation or stabilization of soluble guanylyl cyclase upon haem oxidation-induced degradation.","volume":"157","author":"LS Hoffmann","year":"2009","journal-title":"Br J Pharmacol"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"22651","DOI":"10.1074\/jbc.M110.111559","article-title":"Structure of cinaciguat (BAY 58-2667) bound to Nostoc H-NOX domain reveals insights into heme-mimetic activation of the soluble guanylyl cyclase.","volume":"285","author":"F Martin","year":"2010","journal-title":"J Biol Chem"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"4205","DOI":"10.1016\/j.febslet.2006.06.079","article-title":"Identification of residues crucially involved in soluble guanylate cyclase activation.","volume":"580","author":"C Rothkegel","year":"2006","journal-title":"FEBS Lett"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/j.ab.2004.12.025","article-title":"A cell-based cGMP assay useful for ultra-high-throughput screening and identification of modulators of the nitric oxide\/cGMP pathway.","volume":"339","author":"F Wunder","year":"2005","journal-title":"Anal Biochem"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"10848","DOI":"10.1021\/bi9929296","article-title":"Inhibition of soluble guanylate cyclase by ODQ.","volume":"39","author":"Y Zhao","year":"2000","journal-title":"Biochemistry"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1038\/sj.bjp.0701603","article-title":"Characterization of NS 2028 as a specific inhibitor of soluble guanylyl cyclase.","volume":"123","author":"SP Olesen","year":"1998","journal-title":"Br J Pharmacol"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"8516","DOI":"10.1074\/jbc.M210432200","article-title":"Mitochondrial complex I inhibitor rotenone induces apoptosis through enhancing mitochondrial reactive oxygen species production.","volume":"278","author":"N Li","year":"2003","journal-title":"J Biol Chem"},{"key":"ref24","first-page":"301","article-title":"Soluble guanylate cyclase: allosteric activation and redox regulation.","author":"AJ Hobbs","year":"2009"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1016\/j.febslet.2007.12.025","article-title":"Heat shock protein 90 regulates stabilization rather than activation of soluble guanylate cyclase.","volume":"582","author":"PI Nedvetsky","year":"2008","journal-title":"FEBS Lett"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"534","DOI":"10.1161\/01.RES.85.6.534","article-title":"Downregulation of soluble guanylyl cyclase in young and aging spontaneously hypertensive rats.","volume":"85","author":"H Ruetten","year":"1999","journal-title":"Circ Res"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"16671","DOI":"10.1073\/pnas.0405509101","article-title":"Reduced cGMP signaling associated with neointimal proliferation and vascular dysfunction in late-stage atherosclerosis.","volume":"101","author":"VO Melichar","year":"2004","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1038\/sj.bjp.0704300","article-title":"Downregulation of vascular soluble guanylate cyclase induced by high salt intake in spontaneously hypertensive rats.","volume":"134","author":"S Kagota","year":"2001","journal-title":"Br J Pharmacol"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1007\/978-3-540-68964-5_11","article-title":"Novel techniques for real-time monitoring of cGMP in living cells.","volume":"191","author":"VO Nikolaev","year":"2009","journal-title":"Handb Exp Pharmacol"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1016\/S0162-0134(01)00387-7","article-title":"Role of conformational changes in the heme-dependent regulation of human soluble guanylate cyclase.","volume":"87","author":"DN Kosarikov","year":"2001","journal-title":"J Inorg Biochem"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1134\/S0006297907010099","article-title":"Engineering, expression, purification, and physicochemical characterization of a chimeric protein, full-length cytochrome b(5)\u2013green fluorescence protein (HMWb5\u2013EGFP).","volume":"72","author":"AV Yantsevich","year":"2007","journal-title":"Biochemistry (Mosc)"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/S0167-4838(99)00156-9","article-title":"Conformational dynamics and molecular interaction reactions of recombinant cytochrome p450scc (CYP11A1) detected by fluorescence energy transfer.","volume":"1434","author":"GI Lepesheva","year":"1999","journal-title":"Biochim Biophys Acta"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1126\/science.281.5374.269","article-title":"Specific covalent labeling of recombinant protein molecules inside live cells.","volume":"281","author":"BA Griffin","year":"1998","journal-title":"Science"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"6063","DOI":"10.1021\/ja017687n","article-title":"New biarsenical ligands and tetracysteine motifs for protein labeling in vitro and in vivo: synthesis and biological applications.","volume":"124","author":"SR Adams","year":"2002","journal-title":"J Am Chem Soc"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1038\/nmeth742","article-title":"A FlAsH-based FRET approach to determine G protein-coupled receptor activation in living cells.","volume":"2","author":"C Hoffmann","year":"2005","journal-title":"Nat Methods"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"906","DOI":"10.1038\/nrm976","article-title":"Creating new fluorescent probes for cell biology.","volume":"3","author":"J Zhang","year":"2002","journal-title":"Nat Rev Mol Cell Biol"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"569","DOI":"10.2144\/000112264","article-title":"FlAsH-based live-cell fluorescent imaging of synthetic peptides expressed in Arabidopsis and tobacco.","volume":"41","author":"JM Estevez","year":"2006","journal-title":"Biotechniques"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"17777","DOI":"10.1073\/pnas.0608509103","article-title":"Golgi twins in late mitosis revealed by genetically encoded tags for live cell imaging and correlated electron microscopy.","volume":"103","author":"GM Gaietta","year":"2006","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"10034","DOI":"10.1021\/ja057005m","article-title":"Revealing two-state protein-protein interactions of calmodulin by single-molecule spectroscopy.","volume":"128","author":"R Liu","year":"2006","journal-title":"J Am Chem Soc"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"779","DOI":"10.1038\/nchembio.2007.49","article-title":"Surveying polypeptide and protein domain conformation and association with FlAsH and ReAsH.","volume":"3","author":"NW Luedtke","year":"2007","journal-title":"Nat Chem Biol"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"534","DOI":"10.1124\/mol.108.052399","article-title":"Fluorescence resonance energy transfer analysis of alpha 2a-adrenergic receptor activation reveals distinct agonist-specific conformational changes.","volume":"75","author":"A Zurn","year":"2009","journal-title":"Mol Pharmacol"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1006\/bbrc.2001.5966","article-title":"Design of an artificial light-harvesting unit by protein engineering: cytochrome b(562)-green fluorescent Protein chimera.","volume":"289","author":"S Takeda","year":"2001","journal-title":"Biochem Biophys Res Commun"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1016\/j.ab.2005.11.036","article-title":"A Forster-resonance-energy transfer-based method for fluorescence detection of the protein redox state.","volume":"350","author":"S Kuznetsova","year":"2006","journal-title":"Anal Biochem"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"11348","DOI":"10.1021\/bi8011803","article-title":"Allosteric transitions in cytochrome P450eryF explored with pressure-perturbation spectroscopy, lifetime FRET, and a novel fluorescent substrate, Fluorol-7GA.","volume":"47","author":"DR Davydov","year":"2008","journal-title":"Biochemistry"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"11984","DOI":"10.1073\/pnas.97.22.11984","article-title":"Biochemistry, mutagenesis, and oligomerization of DsRed, a red fluorescent protein from coral.","volume":"97","author":"GS Baird","year":"2000","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"1711","DOI":"10.1016\/S0968-0896(02)00034-2","article-title":"Metabolites of orally active NO-independent pyrazolopyridine stimulators of soluble guanylate cyclase.","volume":"10","author":"A Straub","year":"2002","journal-title":"Bioorg Med Chem"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"1666","DOI":"10.1038\/nprot.2010.129","article-title":"Fluorescent labeling of tetracysteine-tagged proteins in intact cells.","volume":"5","author":"C Hoffmann","year":"2010","journal-title":"Nat Protoc"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"1181","DOI":"10.1124\/mol.107.036368","article-title":"Dimerization region of soluble guanylate cyclase characterized by bimolecular fluorescence complementation in vivo.","volume":"72","author":"C Rothkegel","year":"2007","journal-title":"Mol Pharmacol"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0023596","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,8]],"date-time":"2025-03-08T22:05:49Z","timestamp":1741471549000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0023596"}},"subtitle":[],"editor":[{"given":"Carlo","family":"Gaetano","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2011,8,17]]},"references-count":48,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2011,8,17]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0023596","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,8,17]]}}}