{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T16:07:18Z","timestamp":1787242038762,"version":"build-2736575974"},"reference-count":29,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2011,10,12]],"date-time":"2011-10-12T00:00:00Z","timestamp":1318377600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0025795","type":"journal-article","created":{"date-parts":[[2011,10,12]],"date-time":"2011-10-12T16:50:03Z","timestamp":1318438203000},"page":"e25795","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":29,"title":["Analysis of RNA Binding by the Dengue Virus NS5 RNA Capping Enzyme"],"prefix":"10.1371","volume":"6","author":[{"given":"Brittney R.","family":"Henderson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bejan J.","family":"Saeedi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grace","family":"Campagnola","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian J.","family":"Geiss","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2011,10,12]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1006\/viro.1997.8698","article-title":"Viral and cellular enzymes involved in synthesis of mRNA cap structure.","volume":"236","author":"M Bisaillon","year":"1997","journal-title":"Virology"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"27412","DOI":"10.1074\/jbc.M501393200","article-title":"Modulation of the nucleoside triphosphatase\/RNA helicase and 5\u2032-RNA triphosphatase activities of Dengue virus type 2 nonstructural protein 3 (NS3) by interaction with NS5, the RNA-dependent RNA polymerase.","volume":"280","author":"C Yon","year":"2005","journal-title":"J Biol Chem"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"10278","DOI":"10.1128\/JVI.79.16.10278-10288.2005","article-title":"Structure of the Dengue virus helicase\/nucleoside triphosphatase catalytic domain at a resolution of 2.4 A.","volume":"79","author":"T Xu","year":"2005","journal-title":"J Virol"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"989","DOI":"10.1128\/JVI.67.2.989-996.1993","article-title":"RNA-stimulated NTPase activity associated with yellow fever virus NS3 protein expressed in bacteria.","volume":"67","author":"P Warrener","year":"1993","journal-title":"J Virol"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"8362","DOI":"10.1128\/JVI.00814-06","article-title":"West nile virus 5\u2032-cap structure is formed by sequential Guanine N-7 and ribose 2\u2032-o methylations by nonstructural protein 5.","volume":"80","author":"D Ray","year":"2006","journal-title":"J Virol"},{"issue":"Pt 9","key":"ref6","first-page":"2077","article-title":"Characterization of the NTPase activity of Japanese encephalitis virus NS3 protein.","volume":"77","author":"MD Kuo","year":"1996","journal-title":"J Gen Virol"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"2340","DOI":"10.1261\/rna.1609709","article-title":"The flavivirus NS5 protein is a true RNA guanylyltransferase that catalyzes a two-step reaction to form the RNA cap structure.","volume":"15","author":"M Issur*","year":"2009","journal-title":"RNA"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"2757","DOI":"10.1093\/emboj\/21.11.2757","article-title":"An RNA cap (nucleoside-2\u2032-O-)-methyltransferase in the flavivirus RNA polymerase NS5: crystal structure and functional characterization.","volume":"21","author":"MP Egloff","year":"2002","journal-title":"EMBO J"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1016\/j.virol.2004.07.004","article-title":"The RNA helicase, nucleotide 5\u2032-triphosphatase, and RNA 5\u2032-triphosphatase activities of Dengue virus protein NS3 are Mg2+-dependent and require a functional Walker B motif in the helicase catalytic core.","volume":"328","author":"D Benarroch","year":"2004","journal-title":"Virology"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"10268","DOI":"10.1128\/JVI.79.16.10268-10277.2005","article-title":"Structure of the Flavivirus helicase: implications for catalytic activity, protein interactions, and proteolytic processing.","volume":"79","author":"J Wu","year":"2005","journal-title":"J Virol"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"1643","DOI":"10.1016\/j.jmb.2008.11.058","article-title":"Analysis of flavivirus NS5 methyltransferase cap binding.","volume":"385","author":"BJ Geiss","year":"2009","journal-title":"J Mol Biol"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1006\/viro.2002.1504","article-title":"Expression, purification, and characterization of the RNA 5\u2032-triphosphatase activity of dengue virus type 2 nonstructural protein 3.","volume":"299","author":"G Bartelma","year":"2002","journal-title":"Virology"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"3891","DOI":"10.1128\/JVI.02704-06","article-title":"Structure and function of flavivirus NS5 methyltransferase.","volume":"81","author":"Y Zhou","year":"2007","journal-title":"J Virol"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"846","DOI":"10.4269\/ajtmh.2009.80.846","article-title":"Cost of dengue cases in eight countries in the Americas and Asia: a prospective study.","volume":"80","author":"JA Suaya","year":"2009","journal-title":"Am J Trop Med Hyg"},{"issue":"Pt 4","key":"ref15","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1099\/0022-1317-74-4-733","article-title":"Computer-assisted identification of a putative methyltransferase domain in NS5 protein of flaviviruses and lambda 2 protein of reovirus.","volume":"74","author":"EV Koonin","year":"1993","journal-title":"J Gen Virol"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"4295","DOI":"10.1128\/JVI.02202-07","article-title":"West Nile virus methyltransferase catalyzes two methylations of the viral RNA cap through a substrate-repositioning mechanism.","volume":"82","author":"H Dong","year":"2008","journal-title":"J Virol"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"4412","DOI":"10.1128\/JVI.02455-06","article-title":"Distinct RNA elements confer specificity to flavivirus RNA cap methylation events.","volume":"81","author":"H Dong","year":"2007","journal-title":"J Virol"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.antiviral.2009.03.001","article-title":"Flaviviral methyltransferase\/RNA interaction: structural basis for enzyme inhibition.","volume":"83","author":"M Milani","year":"2009","journal-title":"Antiviral Res"},{"key":"ref19","article-title":"Crystal structure of the dengue virus methyltransferase bound to a 5\u2032-capped octameric RNA.","volume":"5","author":"LJ Yap","year":"2010","journal-title":"PLoS One"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"6222","DOI":"10.1128\/MCB.15.11.6222","article-title":"Mutational analysis of mRNA capping enzyme identifies amino acids involved in GTP binding, enzyme-guanylate formation, and GMP transfer to RNA.","volume":"15","author":"P Cong","year":"1995","journal-title":"Mol Cell Biol"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1016\/j.virol.2003.08.027","article-title":"Identification of two histidines necessary for reovirus mRNA guanylyltransferase activity.","volume":"316","author":"T Qiu","year":"2003","journal-title":"Virology"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"7729","DOI":"10.1128\/JVI.00326-08","article-title":"Formation of guanosine(5\u2032)tetraphospho(5\u2032)adenosine cap structure by an unconventional mRNA capping enzyme of vesicular stomatitis virus.","volume":"82","author":"T Ogino","year":"2008","journal-title":"J Virol"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1128\/JVI.02107-07","article-title":"A conserved motif in region v of the large polymerase proteins of nonsegmented negative-sense RNA viruses that is essential for mRNA capping.","volume":"82","author":"J Li","year":"2008","journal-title":"J Virol"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/0003-9861(91)90257-J","article-title":"A simple method for calculating the dissociation constant of a receptor (or enzyme).unlabeled ligand complex from radioligand displacement measurements.","volume":"284","author":"RL Martin","year":"1991","journal-title":"Arch Biochem Biophys"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"870","DOI":"10.1093\/bioinformatics\/btn020","article-title":"A discrete view on fold space.","volume":"24","author":"MJ Sippl","year":"2008","journal-title":"Bioinformatics"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1093\/bioinformatics\/btm622","article-title":"A note on difficult structure alignment problems.","volume":"24","author":"MJ Sippl","year":"2008","journal-title":"Bioinformatics"},{"key":"ref27","article-title":"PyMol website."},{"key":"ref28","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1016\/S1097-2765(00)80044-1","article-title":"Structural basis for sequence-nonspecific recognition of 5\u2032-capped mRNA by a cap-modifying enzyme.","volume":"1","author":"AE Hodel","year":"1998","journal-title":"Mol Cell"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"2592","DOI":"10.1093\/nar\/22.13.2592","article-title":"Methylphosphonate mapping of phosphate contacts critical for RNA recognition by the human immunodeficiency virus tat and rev proteins.","volume":"22","author":"CE Pritchard","year":"1994","journal-title":"Nucleic Acids Res"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0025795","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,6,25]],"date-time":"2020-06-25T18:33:02Z","timestamp":1593109982000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0025795"}},"subtitle":[],"editor":[{"given":"K.T.","family":"Jeang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2011,10,12]]},"references-count":29,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2011,10,12]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0025795","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,10,12]]}}}