{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T18:18:33Z","timestamp":1770747513013,"version":"3.49.0"},"reference-count":45,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2011,10,18]],"date-time":"2011-10-18T00:00:00Z","timestamp":1318896000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0026304","type":"journal-article","created":{"date-parts":[[2011,10,18]],"date-time":"2011-10-18T21:06:58Z","timestamp":1318972018000},"page":"e26304","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":30,"title":["Broad-Spectrum Antiviral Activity of RNA Interference against Four Genotypes of Japanese Encephalitis Virus Based on Single MicroRNA Polycistrons"],"prefix":"10.1371","volume":"6","author":[{"given":"Zhiqiang","family":"Wu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Xue","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiang","family":"Du","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qi","family":"Jin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2011,10,18]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3201\/eid1501.080311","article-title":"Past, present, and future of Japanese encephalitis.","volume":"15","author":"TE Erlanger","year":"2009","journal-title":"Emerg Infect Dis"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"3635","DOI":"10.1016\/j.vaccine.2010.02.105","article-title":"Phenotypic and genotypic characteristics of Japanese encephalitis attenuated live vaccine virus SA14-14-2 and their stabilities.","volume":"28","author":"Y Yu","year":"2010","journal-title":"Vaccine"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"1047","DOI":"10.1111\/j.1348-0421.2005.tb03701.x","article-title":"Inhibitory effect of RNAi on Japanese encephalitis virus replication in vitro and in vivo.","volume":"49","author":"M Murakami","year":"2005","journal-title":"Microbiol Immunol"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"e624","DOI":"10.1371\/journal.pntd.0000624","article-title":"The neglected arboviral infections in mainland China.","volume":"4","author":"X Gao","year":"2010","journal-title":"PLoS Negl Trop Dis"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1016\/j.antiviral.2007.10.005","article-title":"Does antiviral therapy have a role in the control of Japanese encephalitis?","volume":"78","author":"EA Gould","year":"2008","journal-title":"Antiviral Res"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1038\/nrmicro1067","article-title":"A structural perspective of the flavivirus life cycle.","volume":"3","author":"S Mukhopadhyay","year":"2005","journal-title":"Nat Rev Microbiol"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1016\/j.antiviral.2008.08.004","article-title":"Molecular targets for flavivirus drug discovery.","volume":"81","author":"A Sampath","year":"2009","journal-title":"Antiviral Res"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"876","DOI":"10.3201\/eid1605.091055","article-title":"Molecular epidemiology of Japanese encephalitis virus, Taiwan.","volume":"16","author":"JH Huang","year":"2010","journal-title":"Emerg Infect Dis"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"7131","DOI":"10.1016\/j.vaccine.2009.09.051","article-title":"Molecular epidemiology of Japanese encephalitis in East Asia.","volume":"27","author":"K Morita","year":"2009","journal-title":"Vaccine"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"3091","DOI":"10.1128\/JVI.77.5.3091-3098.2003","article-title":"Origin and evolution of Japanese encephalitis virus in southeast Asia.","volume":"77","author":"T Solomon","year":"2003","journal-title":"J Virol"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"885","DOI":"10.1099\/vir.0.82185-0","article-title":"Molecular epidemiological analysis of Japanese encephalitis virus in China.","volume":"88","author":"HY Wang","year":"2007","journal-title":"J Gen Virol"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1186\/1743-422X-7-127","article-title":"Molecular epidemiology of Japanese encephalitis virus circulating in South Korea, 1983\u20132005.","volume":"7","author":"SM Yun","year":"2010","journal-title":"Virol J"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"642","DOI":"10.1016\/j.cell.2009.01.035","article-title":"Origins and Mechanisms of miRNAs and siRNAs.","volume":"136","author":"RW Carthew","year":"2009","journal-title":"Cell"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1038\/35052556","article-title":"Post-transcriptional gene silencing by double-stranded RNA.","volume":"2","author":"SM Hammond","year":"2001","journal-title":"Nat Rev Genet"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1016\/S0092-8674(02)00908-X","article-title":"Single-stranded antisense siRNAs guide target RNA cleavage in RNAi.","volume":"110","author":"J Martinez","year":"2002","journal-title":"Cell"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"861","DOI":"10.1016\/j.molcel.2004.12.002","article-title":"Transcription and processing of human microRNA precursors.","volume":"16","author":"BR Cullen","year":"2004","journal-title":"Mol Cell"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"494","DOI":"10.1038\/35078107","article-title":"Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells.","volume":"411","author":"SM Elbashir","year":"2001","journal-title":"Nature"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1038\/nbt1052","article-title":"Synthetic shRNAs as potent RNAi triggers.","volume":"23","author":"D Siolas","year":"2005","journal-title":"Nat Biotechnol"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"5515","DOI":"10.1073\/pnas.082117599","article-title":"A DNA vector-based RNAi technology to suppress gene expression in mammalian cells.","volume":"99","author":"G Sui","year":"2002","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"5974","DOI":"10.1016\/j.febslet.2005.08.070","article-title":"Approaches for chemically synthesized siRNA and vector-mediated RNAi.","volume":"579","author":"M Amarzguioui","year":"2005","journal-title":"FEBS Lett"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"6900","DOI":"10.1128\/JVI.78.13.6900-6907.2004","article-title":"RNA interference targeting VP1 inhibits foot-and-mouth disease virus replication in BHK-21 cells and suckling mice.","volume":"78","author":"W Chen","year":"2004","journal-title":"J Virol"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"2811","DOI":"10.1093\/nar\/gkn109","article-title":"Inhibition of HIV-1 by multiple siRNAs expressed from a single microRNA polycistron.","volume":"36","author":"YP Liu","year":"2008","journal-title":"Nucleic Acids Res"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"e5671","DOI":"10.1371\/journal.pone.0005671","article-title":"Small interfering RNA targeting m2 gene induces effective and long term inhibition of influenza A virus replication.","volume":"4","author":"HY Sui","year":"2009","journal-title":"PLoS One"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"1263","DOI":"10.1016\/j.addr.2007.03.022","article-title":"Therapeutic application of RNA interference for hepatitis C virus.","volume":"59","author":"T Watanabe","year":"2007","journal-title":"Adv Drug Deliv Rev"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"e96","DOI":"10.1371\/journal.pmed.0030096","article-title":"A single siRNA suppresses fatal encephalitis induced by two different flaviviruses.","volume":"3","author":"P Kumar","year":"2006","journal-title":"PLoS Med"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1038\/nature05901","article-title":"Transvascular delivery of small interfering RNA to the central nervous system.","volume":"448","author":"P Kumar","year":"2007","journal-title":"Nature"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/j.virusres.2007.11.014","article-title":"Effective inhibition of Japanese encephalitis virus replication by small interfering RNAs targeting the NS5 gene.","volume":"132","author":"WB Qi","year":"2008","journal-title":"Virus Res"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"1435","DOI":"10.1038\/nbt1369","article-title":"RNA interference against viruses: strike and counterstrike.","volume":"25","author":"J Haasnoot","year":"2007","journal-title":"Nat Biotechnol"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"1536","DOI":"10.1038\/gt.2008.147","article-title":"Engineering and optimization of the miR-106b cluster for ectopic expression of multiplexed anti-HIV RNAs.","volume":"15","author":"LA Aagaard","year":"2008","journal-title":"Gene Ther"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"e53","DOI":"10.1093\/nar\/gkl143","article-title":"Polycistronic RNA polymerase II expression vectors for RNA interference based on BIC\/miR-155.","volume":"34","author":"KH Chung","year":"2006","journal-title":"Nucleic Acids Res"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"5421","DOI":"10.1111\/j.1742-4658.2006.05534.x","article-title":"Design of expression vectors for RNA interference based on miRNAs and RNA splicing.","volume":"273","author":"G Du","year":"2006","journal-title":"FEBS J"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1186\/1471-2199-8-98","article-title":"A versatile approach to multiple gene RNA interference using microRNA-based short hairpin RNAs.","volume":"8","author":"X Zhu","year":"2007","journal-title":"BMC Mol Biol"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"1327","DOI":"10.1016\/S1097-2765(02)00541-5","article-title":"Both natural and designed micro RNAs can inhibit the expression of cognate mRNAs when expressed in human cells.","volume":"9","author":"Y Zeng","year":"2002","journal-title":"Mol Cell"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1016\/j.antiviral.2008.06.017","article-title":"Quick identification of effective small interfering RNAs that inhibit the replication of coxsackievirus A16.","volume":"80","author":"Z Wu","year":"2008","journal-title":"Antiviral Res"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1016\/j.antiviral.2009.12.010","article-title":"Effective siRNAs inhibit the replication of novel influenza A (H1N1) virus.","volume":"85","author":"W Zhiqiang","year":"2010","journal-title":"Antiviral Res"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"2601","DOI":"10.1128\/JVI.78.5.2601-2605.2004","article-title":"Human immunodeficiency virus type 1 escapes from RNA interference-mediated inhibition.","volume":"78","author":"AT Das","year":"2004","journal-title":"J Virol"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/j.antiviral.2009.07.012","article-title":"Inhibition of rabies virus replication by multiple artificial microRNAs.","volume":"84","author":"N Israsena","year":"2009","journal-title":"Antiviral Res"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.jconrel.2009.08.028","article-title":"Delivery of nucleic acids with a stearylated (RxR)4 peptide using a non-covalent co-incubation strategy.","volume":"141","author":"T Lehto","year":"2010","journal-title":"J Control Release"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"732","DOI":"10.1016\/j.addr.2009.03.004","article-title":"Lentiviral delivery of short hairpin RNAs.","volume":"61","author":"N Manjunath","year":"2009","journal-title":"Adv Drug Deliv Rev"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"526","DOI":"10.1038\/nmat2444","article-title":"Intravaginal gene silencing using biodegradable polymer nanoparticles densely loaded with small-interfering RNA.","volume":"8","author":"KA Woodrow","year":"2009","journal-title":"Nat Mater"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1038\/nature04688","article-title":"RNAi-mediated gene silencing in non-human primates.","volume":"441","author":"TS Zimmermann","year":"2006","journal-title":"Nature"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"1671","DOI":"10.1093\/nar\/gki312","article-title":"A systematic analysis of the silencing effects of an active siRNA at all single-nucleotide mismatched target sites.","volume":"33","author":"Q Du","year":"2005","journal-title":"Nucleic Acids Res"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"2151","DOI":"10.1128\/JVI.79.4.2151-2159.2005","article-title":"Inhibition of coxsackievirus B3 replication by small interfering RNAs requires perfect sequence match in the central region of the viral positive strand.","volume":"79","author":"J Yuan","year":"2005","journal-title":"J Virol"},{"key":"ref44","first-page":"493","article-title":"Asimplemethod of estimating fifty percent end points.","volume":"27","author":"LJ Reed","year":"1938","journal-title":"Am J Hyg"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/j.bbrc.2004.09.222","article-title":"Validating siRNA using a reporter made from synthetic DNA oligonucleotides.","volume":"325","author":"Q Du","year":"2004","journal-title":"Biochem Biophys Res Commun"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0026304","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2018,10,23]],"date-time":"2018-10-23T08:38:39Z","timestamp":1540283919000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0026304"}},"subtitle":[],"editor":[{"given":"Jianming","family":"Qiu","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2011,10,18]]},"references-count":45,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2011,10,18]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0026304","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,10,18]]}}}