{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T22:14:31Z","timestamp":1790979271136,"version":"4.1.0"},"reference-count":45,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2011,10,19]],"date-time":"2011-10-19T00:00:00Z","timestamp":1318982400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0026327","type":"journal-article","created":{"date-parts":[[2011,10,19]],"date-time":"2011-10-19T20:58:05Z","timestamp":1319057885000},"page":"e26327","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":37,"title":["Mechanism of Disruption of the Amt-GlnK Complex by PII-Mediated Sensing of 2-Oxoglutarate"],"prefix":"10.1371","volume":"6","author":[{"given":"Sarah","family":"Maier","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula","family":"Schleberger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"L\u00fc","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tobias","family":"Wacker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tobias","family":"Pfl\u00fcger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Litz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susana L. A.","family":"Andrade","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2011,10,19]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1080\/09687680701388423","article-title":"The Amt\/Mep\/Rh family of ammonium transport proteins.","volume":"24","author":"SL Andrade","year":"2007","journal-title":"Mol Membr Biol"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"14994","DOI":"10.1073\/pnas.0506254102","article-title":"Crystal structure of the archaeal ammonium transporter Amt-1 from <italic>Archaeoglobus fulgidus<\/italic>.","volume":"102","author":"SL Andrade","year":"2005","journal-title":"Proc Natl Acad Sci USA"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"9638","DOI":"10.1073\/pnas.1003587107","article-title":"Function of human Rh based on structure of RhCG at 2.1 \u00c5.","volume":"107","author":"F Gruswitz","year":"2010","journal-title":"Proc Natl Acad Sci USA"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"1587","DOI":"10.1126\/science.1101952","article-title":"Mechanism of ammonia transport by Amt\/MEP\/Rh: structure of AmtB at 1.35 \u00c5.","volume":"305","author":"S Khademi","year":"2004","journal-title":"Science"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"19279","DOI":"10.1073\/pnas.0709710104","article-title":"Structure of the <italic>Nitrosomonas europaea<\/italic> Rh protein.","volume":"104","author":"X Li","year":"2007","journal-title":"Proc Natl Acad Sci USA"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"19303","DOI":"10.1073\/pnas.0706563104","article-title":"The 1.3-\u00c5 resolution structure of <italic>Nitrosomonas europaea<\/italic> Rh50 and mechanistic implications for NH<sub>3<\/sub> transport by Rhesus family proteins.","volume":"104","author":"D Lupo","year":"2007","journal-title":"Proc Natl Acad Sci USA"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"17090","DOI":"10.1073\/pnas.0406475101","article-title":"The mechanism of ammonia transport based on the crystal structure of AmtB of <italic>Escherichia coli<\/italic>.","volume":"101","author":"L Zheng","year":"2004","journal-title":"Proc Natl Acad Sci USA"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1042\/bj1170405","article-title":"Synthesis of glutamate in <italic>Aerobacter aerogenes<\/italic> by a hitherto unknown route.","volume":"117","author":"DW Tempest","year":"1970","journal-title":"Biochem J"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1146\/annurev.micro.61.080706.093409","article-title":"Nitrogen regulation in bacteria and archaea.","volume":"61","author":"JA Leigh","year":"2007","journal-title":"Annu Rev Microbiol"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"44357","DOI":"10.1074\/jbc.R100055200","article-title":"The story of glutamine synthetase regulation.","volume":"276","author":"ER Stadtman","year":"2001","journal-title":"J Biol Chem"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1128\/MMBR.65.1.80-105.2001","article-title":"P<sub>II<\/sub> signal transduction proteins, pivotal players in microbial nitrogen control.","volume":"65","author":"T Arcondeguy","year":"2001","journal-title":"Microbiol Mol Biol Rev"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1046\/j.1365-2958.1998.00932.x","article-title":"Role of the GlnK signal transduction protein in the regulation of nitrogen assimilation in <italic>Escherichia coli<\/italic>.","volume":"29","author":"MR Atkinson","year":"1998","journal-title":"Mol Microbiol"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1046\/j.1365-2958.1996.6281349.x","article-title":"An alternative P<sub>II<\/sub> protein in the regulation of glutamine synthetase in <italic>Escherichia coli<\/italic>.","volume":"21","author":"WC van Heeswijk","year":"1996","journal-title":"Mol Microbiol"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1046\/j.1365-2958.2002.03153.x","article-title":"Physiological role of the GlnK signal transduction protein of <italic>Escherichia coli<\/italic>: survival of nitrogen starvation.","volume":"46","author":"TA Blauwkamp","year":"2002","journal-title":"Mol Microbiol"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1128\/jb.176.1.84-91.1994","article-title":"The P<sub>II<\/sub> protein in the cyanobacterium <italic>Synechococcus sp<\/italic>. strain PCC 7942 is modified by serine phosphorylation and signals the cellular N-status.","volume":"176","author":"K Forchhammer","year":"1994","journal-title":"J Bacteriol"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1016\/j.mib.2005.02.011","article-title":"P<sub>II<\/sub> signal transduction proteins: sensors of alpha-ketoglutarate that regulate nitrogen metabolism.","volume":"8","author":"AJ Ninfa","year":"2005","journal-title":"Curr Opin Microbiol"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1016\/j.tplants.2009.07.003","article-title":"P<sub>II<\/sub> in higher plants: a modern role for an ancient protein.","volume":"14","author":"RG Uhrig","year":"2009","journal-title":"Trends Plant Sci"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1016\/j.femsre.2003.11.001","article-title":"Global carbon\/nitrogen control by P<sub>II<\/sub> signal transduction in cyanobacteria: from signals to targets.","volume":"28","author":"K Forchhammer","year":"2004","journal-title":"FEMS Microbiol Rev"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"13965","DOI":"10.1073\/pnas.95.23.13965","article-title":"A P<sub>II<\/sub>-like protein in <italic>Arabidopsis<\/italic>: putative role in nitrogen sensing.","volume":"95","author":"MH Hsieh","year":"1998","journal-title":"Proc Natl Acad Sci USA"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"1477","DOI":"10.1021\/bi062149e","article-title":"Crystal Structure of <italic>Arabidopsis<\/italic> P<sub>II<\/sub> Reveals Novel Structural Elements Unique to Plants.","volume":"46","author":"Y Mizuno","year":"2007","journal-title":"Biochemistry"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"17644","DOI":"10.1073\/pnas.0705987104","article-title":"The crystal structure of the complex of P<sub>II<\/sub> and acetylglutamate kinase reveals how P<sub>II<\/sub> controls the storage of nitrogen as arginine.","volume":"104","author":"JL Llacer","year":"2007","journal-title":"Proc Natl Acad Sci USA"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/0014-5793(96)00737-5","article-title":"The role of the T-loop of the signal transducing protein P<sub>II<\/sub> from <italic>Escherichia coli<\/italic>.","volume":"391","author":"R Jaggi","year":"1996","journal-title":"FEBS Lett"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1107\/S1744309110047482","article-title":"Structure of GlnK1, a signalling protein from <italic>Archaeoglobus fulgidus<\/italic>.","volume":"67","author":"C Litz","year":"2011","journal-title":"Acta Crystallogr Sect F Struct Biol Cryst Commun"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/j.jmb.2010.07.020","article-title":"Cooperative binding of MgATP and MgADP in the trimeric P<sub>II<\/sub> protein GlnK2 from <italic>Archaeoglobus fulgidus<\/italic>.","volume":"402","author":"S Helfmann","year":"2010","journal-title":"J Mol Biol"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"536","DOI":"10.1093\/emboj\/21.4.536","article-title":"Membrane sequestration of the signal transduction protein GlnK by the ammonium transporter AmtB.","volume":"21","author":"G Coutts","year":"2002","journal-title":"EMBO J"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"1213","DOI":"10.1073\/pnas.0610348104","article-title":"The crystal structure of the <italic>Escherichia coli<\/italic> AmtB-GlnK complex reveals how GlnK regulates the ammonia channel.","volume":"104","author":"MJ Conroy","year":"2007","journal-title":"Proc Natl Acad Sci USA"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1073\/pnas.0609796104","article-title":"Inhibitory complex of the transmembrane ammonia channel, AmtB, and the cytosolic regulatory protein, GlnK, at 1.96 \u00c5.","volume":"104","author":"F Gruswitz","year":"2007","journal-title":"Proc Natl Acad Sci USA"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"31037","DOI":"10.1074\/jbc.M110.153908","article-title":"Control of AmtB-GlnK complex formation by intracellular levels of ATP, ADP, and 2-oxoglutarate.","volume":"285","author":"MV Radchenko","year":"2010","journal-title":"J Biol Chem"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1042\/bj1520023","article-title":"The contents of adenine nucleotides, phosphagens and some glycolytic intermediates in resting muscles from vertebrates and invertebrates.","volume":"152","author":"I Beis","year":"1975","journal-title":"Biochem J"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"3719","DOI":"10.1016\/S0021-9258(18)95867-9","article-title":"Intracellular Concentration of Bound and Unbound Magnesium Ions in <italic>Escherichia coli<\/italic>.","volume":"242","author":"C Hurwitz","year":"1967","journal-title":"J Biol Chem"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1016\/j.jmb.2010.05.036","article-title":"A new P<sub>II<\/sub> protein structure identifies the 2-oxoglutarate binding site.","volume":"400","author":"D Truan","year":"2010","journal-title":"J Mol Biol"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"19760","DOI":"10.1073\/pnas.1007653107","article-title":"Mechanism of 2-oxoglutarate signaling by the <italic>Synechococcus elongatus<\/italic> P<sub>II<\/sub> signal transduction protein.","volume":"107","author":"O Fokina","year":"2010","journal-title":"Proc Natl Acad Sci USA"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.febslet.2010.11.055","article-title":"AmtB-mediated NH<sub>3<\/sub> transport in prokaryotes must be active and as a consequence regulation of transport by GlnK is mandatory to limit futile cycling of NH<sub>4<\/sub><sup>+<\/sup>\/NH<sub>3<\/sub>.","volume":"585","author":"FC Boogerd","year":"2011","journal-title":"FEBS Lett"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"4255","DOI":"10.1073\/pnas.061034698","article-title":"Futile transmembrane NH<sub>4<\/sub><sup>+<\/sup> cycling: a cellular hypothesis to explain ammonium toxicity in plants.","volume":"98","author":"DT Britto","year":"2001","journal-title":"Proc Natl Acad Sci USA"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"29558","DOI":"10.1074\/jbc.M602477200","article-title":"<italic>In vitro<\/italic> analysis of the <italic>Escherichia coli<\/italic> AmtB-GlnK complex reveals a stoichiometric interaction and sensitivity to ATP and 2-oxoglutarate.","volume":"281","author":"A Durand","year":"2006","journal-title":"J Biol Chem"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1038\/sj.emboj.7601492","article-title":"Structure of GlnK1 with bound effectors indicates regulatory mechanism for ammonia uptake.","volume":"26","author":"O Yildiz","year":"2007","journal-title":"EMBO J"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1046\/j.1365-313X.2003.01634.x","article-title":"Molecular properties of the putative nitrogen sensor P<sub>II<\/sub> from <italic>Arabidopsis thaliana<\/italic>.","volume":"33","author":"CS Smith","year":"2003","journal-title":"Plant J"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"410","DOI":"10.1016\/j.jmb.2010.04.018","article-title":"A novel signal transduction protein P<sub>II<\/sub> variant from <italic>Synechococcus elongatus<\/italic> PCC 7942 indicates a two-step process for NAGK-P<sub>II<\/sub> complex formation.","volume":"399","author":"O Fokina","year":"2010","journal-title":"J Mol Biol"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"34909","DOI":"10.1074\/jbc.M607582200","article-title":"Interaction of the membrane-bound GlnK-AmtB complex with the master regulator of nitrogen metabolism TnrA in <italic>Bacillus subtilis<\/italic>.","volume":"281","author":"A Heinrich","year":"2006","journal-title":"J Biol Chem"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"869","DOI":"10.1111\/j.1432-1033.1997.00869.x","article-title":"Phosphoprotein P<sub>II<\/sub> from cyanobacteria. Analysis of functional conservation with the P<sub>II<\/sub> signal-transduction protein from <italic>Escherichia coli<\/italic>.","volume":"244","author":"K Forchhammer","year":"1997","journal-title":"Eur J Biochem"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"17797","DOI":"10.1074\/jbc.270.30.17797","article-title":"The <italic>Escherichia coli<\/italic> P<sub>II<\/sub> signal transduction protein is activated upon binding 2-ketoglutarate and ATP.","volume":"270","author":"ES Kamberov","year":"1995","journal-title":"J Biol Chem"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of bacteriophage T4.","volume":"227","author":"UK Laemmli","year":"1970","journal-title":"Nature"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"760","DOI":"10.1107\/S0907444994003112","article-title":"The CCP4 suite: programs for protein crystallography.","volume":"50","year":"1994","journal-title":"Acta Crystallogr D Biol Crystallogr"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1107\/S0907444909047337","article-title":"XDS.","volume":"66","author":"W Kabsch","year":"2010","journal-title":"Acta Crystallogr D Biol Crystallogr"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"2126","DOI":"10.1107\/S0907444904019158","article-title":"Coot: model-building tools for molecular graphics.","volume":"60","author":"P Emsley","year":"2004","journal-title":"Acta Crystallogr D Biol Crystallogr"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0026327","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,12,11]],"date-time":"2021-12-11T22:15:45Z","timestamp":1639260945000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0026327"}},"subtitle":[],"editor":[{"given":"Maria","family":"Gasset","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2011,10,19]]},"references-count":45,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2011,10,19]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0026327","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,10,19]]}}}