{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T01:51:28Z","timestamp":1771465888329,"version":"3.50.1"},"reference-count":53,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2012,1,23]],"date-time":"2012-01-23T00:00:00Z","timestamp":1327276800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0030150","type":"journal-article","created":{"date-parts":[[2012,1,23]],"date-time":"2012-01-23T21:59:04Z","timestamp":1327355944000},"page":"e30150","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":13,"title":["Effects of High Hydrostatic Pressure on Bacterial Growth on Human Ossicles Explanted from Cholesteatoma Patients"],"prefix":"10.1371","volume":"7","author":[{"given":"Steffen","family":"Dommerich","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hagen","family":"Frickmann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00fcrgen","family":"Ostwald","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tobias","family":"Lindner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andreas Erich","family":"Zautner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kathleen","family":"Arndt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hans Wilhelm","family":"Pau","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andreas","family":"Podbielski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2012,1,23]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"S73","DOI":"10.1055\/s-2000-15919","article-title":"Grundz\u00fcge einer differenzierten Cholesteatomchirurgie.","volume":"79","author":"H Hildmann","year":"2000","journal-title":"Laryngorhinootologie"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1007\/BF00171523","article-title":"Treatment of cholesteatoma and retraction pockets.","volume":"250","author":"J Sade","year":"1993","journal-title":"Eur Arch Otorhinolaryngol"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"117","DOI":"10.3342\/ceo.2008.1.3.117","article-title":"The Role of Inflammatory Mediators in the Pathogenesis of Otitis Media and Sequelae.","volume":"1","author":"SK Juhn","year":"2008","journal-title":"Clin Exp Otorhinolaryngol"},{"key":"ref4","first-page":"38","article-title":"Current concepts on the pathogenesis of acquired middle ear cholesteatomas.","volume":"90","author":"HJ Welkoborsky","year":"2011","journal-title":"Laryngorhinootologie"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"1129","DOI":"10.1001\/archotol.128.10.1129","article-title":"Evidence for microbial biofilms in cholesteatomas.","volume":"128","author":"RA Chole","year":"2002","journal-title":"Arch Otolaryngol Head Neck Surg"},{"key":"ref6","first-page":"990","article-title":"Autologous tissue in initial type I and type III tympanoplasty operations in chronic suppurative otitis media.","volume":"45","author":"I Baumann","year":"1997","journal-title":"HNO"},{"key":"ref7","first-page":"164","article-title":"Results after rebuilding the ossicular chain using the autogenous incus, ionomer-cement-and titanium implants (tympanoplasty type III).","volume":"81","author":"G Geyer","year":"2002","journal-title":"Laryngorhinootologie"},{"key":"ref8","first-page":"5","article-title":"Ear ossicle transplants for reconstruction of sound transmission in the middle ear. A histologic long-term study.","volume":"71","author":"H Hildmann","year":"1992","journal-title":"Laryngorhinootologie"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1055\/s-2000-293","article-title":"Animal experiments and cell culture studies on stapes reconstruction with diverse biomaterials.","volume":"79","author":"P Dost","year":"2000","journal-title":"Laryngorhinootologie"},{"key":"ref10","first-page":"89","article-title":"Pitfalls in ossicular chain reconstruction.","volume":"53","author":"T Zahnert","year":"2005","journal-title":"HNO"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"1252","DOI":"10.1001\/archotol.1988.01860230046020","article-title":"Can acquired immunodeficiency syndrome and Creutzfeldt-Jakob disease be transmitted via otologic homografts?","volume":"114","author":"ME Glasscock","year":"1988","journal-title":"Arch Otolaryngol Head Neck Surg"},{"key":"ref12","first-page":"3","article-title":"High pressure effects on biological macromolecules: from structural changes to alteration of cellular processes.","volume":"1595","author":"C Balny","year":"2002","journal-title":"Biochem Biophys Acta"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1006\/excr.1996.0278","article-title":"Hydrostatic pressure has different effects on the assembly of tubulin, actin, myosin II, vinculin, talin, vimentin and cytokeratin in mammalian tissue cells.","volume":"227","author":"HC Crenshaw","year":"1996","journal-title":"Exp Cell Res"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1111\/j.1432-1033.1994.tb18774.x","article-title":"Proteins under pressure \u2013 the influence of high hydrostatic pressure on structure, function and assembly of protein complexes.","volume":"221","author":"M Gross","year":"1994","journal-title":"Eur J Biochem"},{"key":"ref15","first-page":"285","article-title":"Effect of high hydrostatic pressure on biological properties of extracellular bone matrix proteins.","volume":"16","author":"P Diehl","year":"2005","journal-title":"Int J Mol Med"},{"key":"ref16","first-page":"1017","article-title":"Growth promoting in vitro effect of synthetic cyclic RGD-peptides on human osteoblast-like cells attached to cancellous bone.","volume":"17","author":"U Magdolen","year":"2006","journal-title":"Int J Mol Med"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1007\/s00776-006-1031-z","article-title":"Biomechanical and immunohistochemical analysis of high hydrostatic pressure-treated Achilles tendons.","volume":"11","author":"P Diehl","year":"2006","journal-title":"J Orthop Sci"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1002\/jbm.b.30343","article-title":"Biomechanical investigation of the effect of high hydrostatic pressure treatment on the mechanical properties of human bone.","volume":"76","author":"E Steinhauser","year":"2006","journal-title":"J Biomed Mat Res B Biomater"},{"key":"ref19","first-page":"284","article-title":"Devitalization of Cholesteatoma on Human Ossicles by Hydrostatic High Pressure Treatment.","volume":"89","author":"S Dommerich","year":"2010","journal-title":"Laryngo Rhino Otol"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0074-7696(01)01001-4","article-title":"Effects of high hydrostatic pressure on living cells: a consequence of the properties of macromolecules and macromolecule-associated water.","volume":"201","author":"P Mentr\u00e9","year":"2001","journal-title":"Int Rev Cytol"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1155\/S1110724301000158","article-title":"High-pressure biotechnology in medicine and pharmaceutical science.","volume":"1","author":"P Masson","year":"2001","journal-title":"J Biomed Biotechnol"},{"key":"ref22","first-page":"1851","article-title":"Induction of tumor cell death by hydrostatic pressure as a novel supporting technique in orthopedic surgery.","volume":"10","author":"P Diehl","year":"2003","journal-title":"Oncol Rep"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1177\/108201329500100203","article-title":"Review: High-pressure, microbial inactivation and food preservation.","volume":"1","author":"JC Cheftel","year":"1995","journal-title":"Food Sci Technol Int"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"4248","DOI":"10.1128\/AEM.65.9.4248-4251.1999","article-title":"Variation in resistance to hydrostatic pressure among strains of food-borne pathogens.","volume":"65","author":"H Alpas","year":"1999","journal-title":"Appl Environ Microbiol"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"e13135","DOI":"10.1371\/journal.pone.0013135","article-title":"Setup of an in vitro test system for basic studies on biofilm behavior of mixed-species cultures with dental and periodontal pathogens.","volume":"5 pii","author":"K Standar","year":"2010","journal-title":"PLoS One"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"2864","DOI":"10.1128\/AEM.72.4.2864-2875.2006","article-title":"Characterization of biofilm formation by clinically relevant serotypes of group A streptococci.","volume":"72","author":"C Lembke","year":"2006","journal-title":"Appl Environ Microbiol"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1053\/jhin.1998.0605","article-title":"Intrinsically vancomycin-resistant gram-positive organisms: clinical relevance and implications for infection control.","volume":"42","author":"RR Nelson","year":"1999","journal-title":"J Hosp Infect"},{"key":"ref28","article-title":"Germination and inactivation of <italic>Bacillus coagulans<\/italic> and <italic>Alicyclobacillus acidoterrestris<\/italic> spores by high hydrostatic pressure treatment in buffer and tomato sauce.","volume":"19","author":"A Vercammen","year":"2011","journal-title":"Int J Food Microbiol Mar"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"2454","DOI":"10.4315\/0362-028X-69.10.2454","article-title":"Inactivation of hepatitis A virus by high-pressure processing: the role of temperature and pressure oscillation.","volume":"69","author":"DH Kingsley","year":"2006","journal-title":"J Food Prot"},{"key":"ref30","first-page":"197","article-title":"Notes on the microbiology of cholesteatoma: clinical findings and treatment.","volume":"29","author":"F Ricciardiello","year":"2009","journal-title":"Acta Otorhinolaryngol Ital"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"945","DOI":"10.1128\/AEM.63.3.945-950.1997","article-title":"<italic>Echerichia coli<\/italic> mutants resistant to inactivation by high hydrostatic pressure.","volume":"63","author":"KJA Hauben","year":"1997","journal-title":"Appl Environ Microbiol"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"6982","DOI":"10.1128\/AEM.00900-10","article-title":"Biological approach to modeling of <italic>Staphylococcus aureus<\/italic> high-hydrostatic-pressure inactivation kinetics.","volume":"76","author":"G Cebri\u00e1n","year":"2010","journal-title":"Appl Environ Microbiol"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"2007","DOI":"10.4315\/0362-028X-71.10.2007","article-title":"Inactivation kinetics of three <italic>Listeria monocytogenes<\/italic> strains under high hydrostatic pressure.","volume":"71","author":"IK Van Boeijen","year":"2008","journal-title":"J Food Prot"},{"key":"ref34","first-page":"117","article-title":"Effects of pressure on lactic acid bacteria. In: Michiels C, Bartlett DH, Aertsen A. High-Pressure Microbiology.","author":"RF Vogel","year":"2008","journal-title":"Am Soc Microbiol"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"1","DOI":"10.2174\/1874325000903010001","article-title":"High hydrostatic pressure for disinfection of bone grafts and biomaterials: an experimental study.","volume":"29; 3","author":"H Gollwitzer","year":"2009","journal-title":"Open Orthop J"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1242\/jcs.3.2.273","article-title":"Fine structure and radiation resistance in Acinetobacter: Studies on a resistant strain.","volume":"3","author":"MJ Thornley","year":"1968","journal-title":"J Cell Sci"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"2225","DOI":"10.1128\/AEM.02434-09","article-title":"Population diversity of <italic>Listeria monocytogenes<\/italic> LO28: phenotypic and genotypic characterization of variants resistant to high hydrostatic pressure.","volume":"76","author":"IK Van Boeijen","year":"2010","journal-title":"Appl Environ Microbiol"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"1878","DOI":"10.1002\/pmic.200402086","article-title":"Comparative approach to characterize the high pressure stress response of <italic>Lactobacillus sanfranciscensis<\/italic> DSM 20451T.","volume":"6","author":"S H\u00f6rmann","year":"2006","journal-title":"Proteomics"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"6298","DOI":"10.1128\/AEM.01316-08","article-title":"The deep-sea bacterium <italic>Photobacterium profundum<\/italic> SS9 utilizes separate flagellar systems for swimming and swarming under high-pressure conditions.","volume":"74","author":"EA Eloe","year":"2008","journal-title":"Appl Environ Microbiol"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1007\/s00203-005-0021-4","article-title":"Transcriptional response reveals translation machinery as target for high pressure in <italic>Lactobacillus sanfranciscensis<\/italic>.","volume":"184","author":"M Pavlovic","year":"2005","journal-title":"Arch Microbiol"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1111\/j.1472-765X.2008.02511.x","article-title":"Impacts of treatment parameters on the inactivation of <italic>Campylobacter jejuni<\/italic> by high pressure: a statistical study of main effects and interactions.","volume":"48","author":"C Bi\u00e8che","year":"2009","journal-title":"Lett Appl Microbiol"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1002\/jbm.b.31408","article-title":"Inactivation of <italic>Staphylococcus aureus<\/italic> in calcium phosphate biomaterials via isostatic compression.","volume":"91","author":"M Brouillet","year":"2009","journal-title":"J Biomed Mater Res B Appl Biomater"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"6307","DOI":"10.1128\/AEM.69.10.6307-6310.2003","article-title":"Effects of minerals on resistance of <italic>Bacillus subtilis<\/italic> spores to heat and hydrostatic pressure.","volume":"69","author":"N Igura","year":"2003","journal-title":"Appl Environ Microbiol"},{"key":"ref44","first-page":"105","article-title":"Inactivation and recovery of <italic>Listeria monocytogenes<\/italic>, <italic>Salmonella enterica<\/italic> and <italic>Staphylococcus aureus<\/italic> after high hydrostatic pressure treatments up to 900 MPa.","volume":"13","author":"A Jofr\u00e9","year":"2010","journal-title":"Int Microbiol"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/j.ijfoodmicro.2006.05.008","article-title":"pH and solute concentration of suspension media affect the outcome of high hydrostatic pressure treatment of <italic>Listeria monocytogenes<\/italic>.","volume":"111","author":"S Koseki","year":"2006","journal-title":"Int J Food Microbiol"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1111\/j.1365-2672.2009.04639.x","article-title":"High hydrostatic pressure resistance of <italic>Campylobacter jejuni<\/italic> after different sublethal stresses.","volume":"109","author":"N Sagarzazu","year":"2010","journal-title":"J Appl Microbiol"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0168-1605(03)00070-9","article-title":"High sucrose concentration protects <italic>E. coli<\/italic> against high pressure inactivation but not against high pressure sensitization to the lactoperoxidase system.","volume":"88","author":"I Van Opstal","year":"2003","journal-title":"Int J Food Microbiol"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"1581","DOI":"10.1128\/AEM.01942-08","article-title":"Changes in barotolerance, thermotolerance, and cellular morphology throughout the life cycle of <italic>Listeria monocytogenes<\/italic>.","volume":"75","author":"J Wen","year":"2009","journal-title":"Appl Environ Microbiol"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1515\/BMT.2008.027","article-title":"Extracorporeal high hydrostatic pressure as a new technology for the disinfection of infected bone specimens.","volume":"53","author":"P Weber","year":"2008","journal-title":"Biomed Tech (Berl)"},{"key":"ref50","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1001\/jama.296.2.202","article-title":"Direct detection of bacterial biofilms on the middle-ear mucosa of children with chronic otitis media.","volume":"296","author":"L Hall-Stoodley","year":"2006","journal-title":"JAMA"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1097\/01.moo.0000124936.46948.6a","article-title":"The role of biofilms in otolaryngologic infections.","volume":"12","author":"JC Post","year":"2004","journal-title":"Curr Opin Otolaryngol Head Neck Surg"},{"key":"ref52","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1016\/j.amjoto.2008.07.007","article-title":"Biofilm formation by <italic>Pseudomonas aeruginosa<\/italic> on ossicular reconstruction prostheses.","volume":"30","author":"EM Jaryszak","year":"2009","journal-title":"Am J Otolaryngol"},{"key":"ref53","doi-asserted-by":"crossref","first-page":"1191","DOI":"10.1097\/MAO.0b013e3181be64b3","article-title":"Effect of ossicular prosthesis biofilms on middle ear scarring and hearing outcomes.","volume":"30","author":"EM Jaryszak","year":"2009","journal-title":"Otol Neurotol"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0030150","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,12,26]],"date-time":"2021-12-26T20:16:25Z","timestamp":1640549785000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0030150"}},"subtitle":[],"editor":[{"given":"Markus M.","family":"Heimesaat","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2012,1,23]]},"references-count":53,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2012,1,23]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0030150","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,1,23]]}}}