{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T05:13:21Z","timestamp":1782105201386,"version":"3.54.5"},"reference-count":49,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2012,1,19]],"date-time":"2012-01-19T00:00:00Z","timestamp":1326931200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0030255","type":"journal-article","created":{"date-parts":[[2012,1,19]],"date-time":"2012-01-19T21:49:12Z","timestamp":1327009752000},"page":"e30255","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":27,"title":["Decreased Autocrine EGFR Signaling in Metastatic Breast Cancer Cells Inhibits Tumor Growth in Bone and Mammary Fat Pad"],"prefix":"10.1371","volume":"7","author":[{"given":"Nicole K.","family":"Nickerson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khalid S.","family":"Mohammad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer L.","family":"Gilmore","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erin","family":"Crismore","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela","family":"Bruzzaniti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theresa A.","family":"Guise","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Foley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2012,1,19]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.pharmthera.2008.11.008","article-title":"Functional selectivity of EGF family peptide growth factors: implications for cancer.","volume":"122","author":"KJ Wilson","year":"2009","journal-title":"Pharmacol Ther"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"2411","DOI":"10.1093\/emboj\/cdg231","article-title":"TACE cleavage of proamphiregulin regulates GPCR-induced proliferation and motility of cancer cells.","volume":"22","author":"A Gschwind","year":"2003","journal-title":"Embo J"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"8503","DOI":"10.1074\/jbc.M413919200","article-title":"Epigen, the last ligand of ErbB receptors, reveals intricate relationships between affinity and mitogenicity.","volume":"280","author":"BS Kochupurakkal","year":"2005","journal-title":"J Biol Chem"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"1115","DOI":"10.1111\/j.1600-0854.2009.00943.x","article-title":"Differential effects of EGFR ligands on endocytic sorting of the receptor.","volume":"10","author":"K Roepstorff","year":"2009","journal-title":"Traffic"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1016\/j.cellsig.2008.10.003","article-title":"Altered EGFR localization and degradation in human breast cancer cells with an amphiregulin\/EGFR autocrine loop.","volume":"21","author":"NE Willmarth","year":"2009","journal-title":"Cell Signal"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"37728","DOI":"10.1074\/jbc.M606532200","article-title":"Autocrine and juxtacrine effects of amphiregulin on the proliferative, invasive, and migratory properties of normal and neoplastic human mammary epithelial cells.","volume":"281","author":"NE Willmarth","year":"2006","journal-title":"J Biol Chem"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"10869","DOI":"10.1073\/pnas.191367098","article-title":"Gene expression patterns of breast carcinomas distinguish tumor subclasses with clinical implications.","volume":"98","author":"T Sorlie","year":"2001","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"530","DOI":"10.1038\/415530a","article-title":"Gene expression profiling predicts clinical outcome of breast cancer.","volume":"415","author":"LJ van 't Veer","year":"2002","journal-title":"Nature"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"1999","DOI":"10.1056\/NEJMoa021967","article-title":"A gene-expression signature as a predictor of survival in breast cancer.","volume":"347","author":"MJ van de Vijver","year":"2002","journal-title":"N Engl J Med"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1093\/jnci\/djq526","article-title":"Associations of breast cancer risk factors with tumor subtypes: a pooled analysis from the Breast Cancer Association Consortium studies.","volume":"103","author":"XR Yang","journal-title":"J Natl Cancer Inst"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"1684","DOI":"10.1200\/JCO.2009.24.9284","article-title":"Breast cancer subtypes and the risk of local and regional relapse.","volume":"28","author":"KD Voduc","journal-title":"J Clin Oncol"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1172\/JCI29518","article-title":"Targeting TACE-dependent EGFR ligand shedding in breast cancer.","volume":"117","author":"PA Kenny","year":"2007","journal-title":"J Clin Invest"},{"key":"ref13","article-title":"EGFR signaling in breast cancer: Bad to the bone.","author":"J Foley","journal-title":"Semin Cell Dev Biol"},{"key":"ref14","article-title":"ADAMTS1 and MMP1 proteolytically engage EGF-like ligands in an osteolytic signaling cascade for bone metastasis.","author":"X Lu","year":"2009","journal-title":"Genes Dev"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"6213s","DOI":"10.1158\/1078-0432.CCR-06-1007","article-title":"Basic mechanisms responsible for osteolytic and osteoblastic bone metastases.","volume":"12","author":"TA Guise","year":"2006","journal-title":"Clin Cancer Res"},{"key":"ref16","article-title":"Genes that mediate breast cancer metastasis to the brain.","author":"PD Bos","year":"2009","journal-title":"Nature"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"765","DOI":"10.1038\/nature05760","article-title":"Mediators of vascular remodelling co-opted for sequential steps in lung metastasis.","volume":"446","author":"GP Gupta","year":"2007","journal-title":"Nature"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"1544","DOI":"10.1172\/JCI118947","article-title":"Evidence for a causal role of parathyroid hormone-related protein in the pathogenesis of human breast cancer-mediated osteolysis.","volume":"98","author":"TA Guise","year":"1996","journal-title":"J Clin Invest"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"4451","DOI":"10.1210\/endo.140.10.7037","article-title":"Breast cancer cells interact with osteoblasts to support osteoclast formation.","volume":"140","author":"RJ Thomas","year":"1999","journal-title":"Endocrinology"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1007\/s10549-007-9748-8","article-title":"Amphiregulin-EGFR signaling regulates PTHrP gene expression in breast cancer cells.","volume":"110","author":"JL Gilmore","year":"2008","journal-title":"Breast Cancer Res Treat"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"632","DOI":"10.1002\/jcb.20550","article-title":"Stimulation of amphiregulin expression in osteoblastic cells by parathyroid hormone requires the protein kinase A and cAMP response element-binding protein signaling pathway.","volume":"96","author":"L Qin","year":"2005","journal-title":"J Cell Biochem"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"19723","DOI":"10.1074\/jbc.M212226200","article-title":"Gene expression profiles and transcription factors involved in parathyroid hormone signaling in osteoblasts revealed by microarray and bioinformatics.","volume":"278","author":"L Qin","year":"2003","journal-title":"J Biol Chem"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"730","DOI":"10.1002\/jcp.21268","article-title":"HMGB1 is a bone-active cytokine.","volume":"214","author":"J Yang","year":"2008","journal-title":"J Cell Physiol"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1002\/ijc.2910520117","article-title":"Single-steep transformation of human breast epithelial cells by SV40 large T oncogene.","volume":"52","author":"P Berthon","year":"1992","journal-title":"Int J Cancer"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"1215","DOI":"10.1369\/jhc.6A6959.2006","article-title":"Three-dimensional reconstruction of fracture callus morphogenesis.","volume":"54","author":"LC Gerstenfeld","year":"2006","journal-title":"J Histochem Cytochem"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1007\/978-1-59745-104-8_3","article-title":"Assessing new bone formation in neonatal calvarial organ cultures.","volume":"455","author":"KS Mohammad","year":"2008","journal-title":"Methods Mol Biol"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"1009","DOI":"10.1016\/S0002-9440(10)62322-X","article-title":"Amphiregulin and epidermal hyperplasia: amphiregulin is required to maintain the psoriatic phenotype of human skin grafts on severe combined immunodeficient mice.","volume":"166","author":"N Bhagavathula","year":"2005","journal-title":"Am J Pathol"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"1373","DOI":"10.1016\/j.joms.2009.03.048","article-title":"Higher bone matrix density exists in only a subset of patients with bisphosphonate-related osteonecrosis of the jaw.","volume":"67","author":"MR Allen","year":"2009","journal-title":"J Oral Maxillofac Surg"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"3644","DOI":"10.1128\/MCB.00851-08","article-title":"Dynamin reduces Pyk2 Y402 phosphorylation and SRC binding in osteoclasts.","volume":"29","author":"A Bruzzaniti","year":"2009","journal-title":"Mol Cell Biol"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1210\/mend-1-3-216","article-title":"Epidermal growth factor receptor gene expression in estrogen receptor-positive and negative human breast cancer cell lines.","volume":"1","author":"NE Davidson","year":"1987","journal-title":"Mol Endocrinol"},{"key":"ref31","first-page":"5475","article-title":"Epidermal growth factor promotes MDA-MB-231 breast cancer cell migration through a phosphatidylinositol 3\u2032-kinase and phospholipase C-dependent mechanism.","volume":"59","author":"JT Price","year":"1999","journal-title":"Cancer Res"},{"key":"ref32","first-page":"8887","article-title":"Epidermal growth factor receptor (HER1) tyrosine kinase inhibitor ZD1839 (Iressa) inhibits HER2\/neu (erbB2)-overexpressing breast cancer cells in vitro and in vivo.","volume":"61","author":"SL Moulder","year":"2001","journal-title":"Cancer Res"},{"key":"ref33","first-page":"1459","article-title":"Inhibition of growth factor production and angiogenesis in human cancer cells by ZD1839 (Iressa), a selective epidermal growth factor receptor tyrosine kinase inhibitor.","volume":"7","author":"F Ciardiello","year":"2001","journal-title":"Clin Cancer Res"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"5278","DOI":"10.1158\/0008-5472.CAN-04-1853","article-title":"Macrophages promote the invasion of breast carcinoma cells via a colony-stimulating factor-1\/epidermal growth factor paracrine loop.","volume":"65","author":"S Goswami","year":"2005","journal-title":"Cancer Res"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"1970","DOI":"10.4049\/jimmunol.1100841","article-title":"Hypoxia-Inducible Factor-2{alpha} Regulates GM-CSF-Derived Soluble Vascular Endothelial Growth Factor Receptor 1 Production from Macrophages and Inhibits Tumor Growth and Angiogenesis.","volume":"187","author":"JM Roda","journal-title":"J Immunol"},{"key":"ref36","article-title":"G-CSF supplementation with chemotherapy can promote revascularization and subsequent tumor regrowth: prevention by a CXCR4 antagonist.","author":"T Voloshin","journal-title":"Blood"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1038\/35036374","article-title":"Matrix metalloproteinase-9 triggers the angiogenic switch during carcinogenesis.","volume":"2","author":"G Bergers","year":"2000","journal-title":"Nat Cell Biol"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1186\/bcr1306","article-title":"Key stages of mammary gland development: molecular mechanisms involved in the formation of the embryonic mammary gland.","volume":"7","author":"JR Hens","year":"2005","journal-title":"Breast Cancer Res"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"2739","DOI":"10.1242\/dev.126.12.2739","article-title":"Targeted inactivation of the EGF and amphiregulin genes reveals distinct roles for EGF receptor ligands in mouse mammary gland development.","volume":"126","author":"NC Luetteke","year":"1999","journal-title":"Development"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"5455","DOI":"10.1073\/pnas.0611647104","article-title":"Amphiregulin is an essential mediator of estrogen receptor alpha function in mammary gland development.","volume":"104","author":"L Ciarloni","year":"2007","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref41","first-page":"777","article-title":"Activation and function of the epidermal growth factor receptor and erbB-2 during mammary gland morphogenesis.","volume":"9","author":"J Sebastian","year":"1998","journal-title":"Cell Growth Differ"},{"key":"ref42","article-title":"Erlotinib inhibits osteolytic bone invasion of human non-small-cell lung cancer cell line NCI-H292.","author":"K Furugaki","year":"2011","journal-title":"Clin Exp Metastasis"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"1714","DOI":"10.1158\/1541-7786.MCR-09-0131","article-title":"Reconstitution of amphiregulin-epidermal growth factor receptor signaling in lung squamous cell carcinomas activates PTHrP gene expression and contributes to cancer-mediated diseases of the bone.","volume":"7","author":"JL Gilmore","year":"2009","journal-title":"Mol Cancer Res"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1038\/sj.bjc.6603828","article-title":"Inhibition of epidermal growth factor receptor signalling reduces hypercalcaemia induced by human lung squamous-cell carcinoma in athymic mice.","volume":"97","author":"G Lorch","year":"2007","journal-title":"Br J Cancer"},{"key":"ref45","first-page":"3961","article-title":"Effect of estrogen, tamoxifen and epidermal growth factor on the transcriptional regulation of vascular endothelial growth factor in breast cancer cells.","volume":"24","author":"JE Lee","year":"2004","journal-title":"Anticancer Res"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"1056","DOI":"10.1038\/sj.bjc.6604634","article-title":"A new role for tamoxifen in oestrogen receptor-negative breast cancer when it is combined with epigallocatechin gallate.","volume":"99","author":"MJ Scandlyn","year":"2008","journal-title":"Br J Cancer"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"1103","DOI":"10.1002\/jcb.22505","article-title":"Compartmentalization of EGFR in cellular membranes: role of membrane rafts.","volume":"109","author":"A Balbis","year":"2010","journal-title":"J Cell Biochem"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"1141","DOI":"10.1038\/nm.2217","article-title":"Pharmacological inhibition of EGFR signaling enhances G-CSF-induced hematopoietic stem cell mobilization.","volume":"16","author":"MA Ryan","year":"2011","journal-title":"Nat Med"},{"key":"ref49","article-title":"Roles of Bone Marrow Cells in Skeletal Metastases: No Longer Bystanders.","author":"SI Park","year":"2011","journal-title":"Cancer Microenviron"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0030255","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,4,18]],"date-time":"2024-04-18T01:25:58Z","timestamp":1713403558000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0030255"}},"subtitle":[],"editor":[{"given":"Pranela","family":"Rameshwar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2012,1,19]]},"references-count":49,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2012,1,19]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0030255","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,1,19]]}}}