{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T14:45:22Z","timestamp":1790779522375,"version":"4.1.0"},"reference-count":42,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2012,1,27]],"date-time":"2012-01-27T00:00:00Z","timestamp":1327622400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0030775","type":"journal-article","created":{"date-parts":[[2012,1,27]],"date-time":"2012-01-27T23:52:02Z","timestamp":1327708322000},"page":"e30775","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":38,"title":["Time-Lapse Imaging of the Dynamics of CNS Glial-Axonal Interactions In Vitro and Ex Vivo"],"prefix":"10.1371","volume":"7","author":[{"given":"Kalliopi","family":"Ioannidou","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kurt I.","family":"Anderson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Strachan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia M.","family":"Edgar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan C.","family":"Barnett","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2012,1,27]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1083\/jcb.8.2.431","article-title":"The formation and structure of myelin sheaths in the central nervous system.","volume":"8","author":"A Peters","year":"1960","journal-title":"J Biophys Bioch Cytol"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"883","DOI":"10.1007\/BF01186817","article-title":"Relations between axons and oligodendroglial cells during initial myelination. II. The individual axon.","volume":"19","author":"S Remahl","year":"1990","journal-title":"J Neurocytol"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"839","DOI":"10.1038\/nrn2480","article-title":"Remyelination in the CNS: from biology to therapy.","volume":"9","author":"RJ Franklin","year":"2008","journal-title":"Nat Rev Neurosci"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"1691","DOI":"10.1002\/glia.20885","article-title":"Role of the oligodendroglial cytoskeleton in differentiation and myelination.","volume":"57","author":"NG Bauer","year":"2009","journal-title":"Glia"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1586\/epr.09.105","article-title":"Myelin matters: proteomic insights into white matter disorders.","volume":"7","author":"HB Werner","year":"2010","journal-title":"Exp Rev Prot"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/0962-8924(93)90213-K","article-title":"The oligodendrocyte and its many cellular processes.","volume":"3","author":"SE Pfeiffer","year":"1993","journal-title":"Trends Cell Biol"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"390","DOI":"10.1038\/303390a0","article-title":"A glial progenitor cell that develops in vitro into an astrocyte or an oligodendrocyte depending on culture medium.","volume":"303","author":"MC Raff","year":"1983","journal-title":"Nature"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"14375","DOI":"10.1523\/JNEUROSCI.4456-07.2007","article-title":"Olig1 and Sox10 interact synergistically to drive myelin basic protein transcription in oligodendrocytes.","volume":"27","author":"H Li","year":"2007","journal-title":"J Neurosci"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"1506","DOI":"10.1038\/nn1803","article-title":"In vivo time-lapse imaging shows dynamic oligodendrocyte progenitor behavior during zebrafish development.","volume":"9","author":"B Kirby","year":"2006","journal-title":"Nature Neurosci"},{"key":"ref10","first-page":"10525","article-title":"Dissecting mechanisms of myelinated axon formation using zebrafish.","author":"T Czopka","year":"2011","journal-title":"Methods Cell Biol"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1016\/j.neuron.2008.09.011","article-title":"Distinct stages of myelination regulated by gamma-secretase and astrocytes in a rapidly myelinating CNS coculture system.","volume":"60","author":"TA Watkins","year":"2008","journal-title":"Neuron"},{"key":"ref12","doi-asserted-by":"crossref","DOI":"10.1002\/glia.21228","article-title":"CNS live imaging reveals a new mechanism of myelination: the liquid croissant model.","author":"B Sobottka","year":"2011"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"3480","DOI":"10.1002\/jnr.22004","article-title":"Disposition of axonal caspr with respect to glial cell membranes: Implications for the process of myelination.","volume":"87","author":"L Pedraza","year":"2009","journal-title":"J Neurosci Res"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1016\/S0014-5793(97)00313-X","article-title":"Green mice' as a source of ubiquitous green cells.","volume":"407","author":"M Okabe","year":"1997","journal-title":"FEBS Letts"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1093\/oxfordjournals.jhered.a109442","article-title":"Shiverer: an autosomal recessive mutant mouse with myelin deficiency.","volume":"72","author":"GF Chernoff","year":"1981","journal-title":"The J Hered"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/S0896-6273(00)00084-2","article-title":"Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP.","volume":"28","author":"G Feng","year":"2000","journal-title":"Neuron"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"750","DOI":"10.1002\/glia.20650","article-title":"Astrocytes, but not olfactory ensheathing cells or Schwann cells, promote myelination of CNS axons in vitro.","volume":"56","author":"A Sorensen","year":"2008","journal-title":"Glia"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"1703","DOI":"10.1002\/jnr.21084","article-title":"Murine spinal cord explants: a model for evaluating axonal growth and myelination in vitro.","volume":"84","author":"CE Thomson","year":"2006","journal-title":"J Neurosci Res"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1073\/pnas.76.1.514","article-title":"Growth of a rat neuroblastoma cell line in serum-free supplemented medium.","volume":"76","author":"JE Bottenstein","year":"1979","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"692","DOI":"10.1111\/j.1471-4159.1985.tb12870.x","article-title":"Molecular genetic analysis of myelin-deficient mice: shiverer mutant mice show deletion in gene(s) coding for myelin basic protein.","volume":"44","author":"M Kimura","year":"1985","journal-title":"J Neurochem"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"7542","DOI":"10.1073\/pnas.83.19.7542","article-title":"Recombination within the myelin basic protein gene created the dysmyelinating shiverer mouse mutation.","volume":"83","author":"SM Molineaux","year":"1986","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/0304-3940(79)91490-3","article-title":"Immunochemical studies of myelin basic protein in shiverer mouse devoid of major dense line of myelin.","volume":"12","author":"P Dupouey","year":"1979","journal-title":"Neurosci Letts"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"1680","DOI":"10.1111\/j.1471-4159.1983.tb08142.x","article-title":"The effect of the shiverer mutation on myelin basic protein expression in homozygous and heterozygous mouse brain.","volume":"40","author":"EML Barbarese","year":"1983","journal-title":"J Neurochem"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/0304-3940(79)91489-7","article-title":"Absence of the major dense line in myelin of the mutant mouse \u201cshiverer\u201d.","volume":"12","author":"A Privat","year":"1979","journal-title":"Neurosci Letts"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"1518","DOI":"10.1111\/j.1460-9568.2008.06415.x","article-title":"Myelinated, synapsing cultures of murine spinal cord- valisdation as an in vitro moels of the central nmervous system.","volume":"28","author":"CE Thomson","year":"2008","journal-title":"E J Neurosci"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1083\/jcb.200312012","article-title":"Oligodendroglial modulation of fast axonal transport in a mouse model of hereditary spastic paraplegia.","volume":"166","author":"JM Edgar","year":"2004","journal-title":"J Cell Biol"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"2143","DOI":"10.1523\/JNEUROSCI.19-06-02143.1999","article-title":"Proteolipid protein gene product can be secreted and exhibit biological activity during early development.","volume":"19","author":"M Yamada","year":"1999","journal-title":"J Nurosci"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1016\/0012-1606(81)90477-2","article-title":"Monoclonal antibodies (O1 to O4) to oligodendrocyte cell surfaces: an immunocytological study in the central nervous system.","volume":"83","author":"I Sommer","year":"1981","journal-title":"Dev Biol"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"1645","DOI":"10.1038\/nprot.2008.160","article-title":"Ex vivo imaging of motor axon dynamics in murine triangularis sterni explants.","volume":"3","author":"M Kerschensteiner","year":"2008","journal-title":"Nature Prot"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"598","DOI":"10.1083\/jcb.95.2.598","article-title":"Synthesis and incorporation of myelin polypeptides into CNS myelin.","volume":"95","author":"DR Colman","year":"1982","journal-title":"J Cell Biol"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"937","DOI":"10.1083\/jcb.200509022","article-title":"Neuron to glia signaling triggers myelin membrane exocytosis from endosomal storage sites.","volume":"172","author":"K Trajkovic","year":"2006","journal-title":"J Cell Biol"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"9174","DOI":"10.1523\/JNEUROSCI.5942-08.2009","article-title":"An integrin-contactin complex regulates CNS myelination by differential Fyn phosphorylation.","volume":"29","author":"LS Laursen","year":"2009","journal-title":"J Neurosci"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/cne.901420102","article-title":"A quantitative study of morphological changes accompanying the initiation and progress of myelin production in the dorsal funiculus of the rat spinal cord.","volume":"142","author":"MA Matthews","year":"1971","journal-title":"J Comp Neurol"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1083\/jcb.12.2.448","article-title":"Electron microscopic demonstration of connections between glia and myelin sheaths in the developing mammalian central nervous system.","volume":"12","author":"MB Bunge","year":"1962","journal-title":"J Cell Biol"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1016\/S0022-5320(64)80104-0","article-title":"The Spiral Configuration of Myelin Lamellae.","volume":"11","author":"BG Uzman","year":"1964","journal-title":"J Ultrast Res"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1083\/jcb.34.2.555","article-title":"A structural analysis of the myelin sheath in the central nervous system.","volume":"34","author":"A Hirano","year":"1967","journal-title":"J Cell Biol"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1016\/S0022-5320(76)80097-4","article-title":"Nonuniformity of the oligodendroglial ensheathment of axons during myelination in the developing rat central nervous system. A serial section electron microscopical study.","volume":"55","author":"RL Knobler","year":"1976","journal-title":"J Ultrastruct Res"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1083\/jcb.48.2.348","article-title":"The geometry of peripheral myelin sheaths during their formation and growth in rat sciatic nerves.","volume":"48","author":"HD Webster","year":"1971","journal-title":"J Cell Biol"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1083\/jcb.2.6.777","article-title":"Formation of myelin in the central nervous system of mice and rats, as studied with the electron microscope.","volume":"2","author":"SA Luse","year":"1956","journal-title":"J Biophys Biochem Cytol"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1083\/jcb.10.1.67","article-title":"Ultrastructure study of remyelination in an experimetnal lesion in adult spinla cord.","volume":"10","author":"MB Bunge","year":"1961","journal-title":"J Biophys Biochem Cytol"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"1012","DOI":"10.1016\/j.cell.2011.06.010","article-title":"Life at the leading edge.","volume":"145","author":"AJ Ridley","year":"2011","journal-title":"Cell"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1038\/nrn1743","article-title":"Mechanisms of axon ensheathment and myelin growth.","volume":"6","author":"DL Sherman","year":"2005","journal-title":"Nat Rev Neurosci"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0030775","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,6,23]],"date-time":"2019-06-23T00:18:13Z","timestamp":1561249093000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0030775"}},"subtitle":[],"editor":[{"given":"Brahim","family":"Nait-Oumesmar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2012,1,27]]},"references-count":42,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2012,1,27]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0030775","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,1,27]]}}}