{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T06:48:26Z","timestamp":1792738106193,"version":"4.5.0"},"reference-count":55,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2012,2,24]],"date-time":"2012-02-24T00:00:00Z","timestamp":1330041600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0031359","type":"journal-article","created":{"date-parts":[[2012,2,24]],"date-time":"2012-02-24T21:54:38Z","timestamp":1330120478000},"page":"e31359","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":79,"title":["Visualization of Murine Intranasal Dosing Efficiency Using Luminescent Francisella tularensis: Effect of Instillation Volume and Form of Anesthesia"],"prefix":"10.1371","volume":"7","author":[{"given":"Mark A.","family":"Miller","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer M.","family":"Stabenow","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jyothi","family":"Parvathareddy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew J.","family":"Wodowski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas P.","family":"Fabrizio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaowen R.","family":"Bina","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lillian","family":"Zalduondo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James E.","family":"Bina","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2012,2,24]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"984","DOI":"10.1152\/japplphysiol.91376.2008","article-title":"An improved simple method of mouse lung intubation.","volume":"106","author":"KD MacDonald","year":"2009","journal-title":"J Appl Physiol"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1258\/la.2007.006068","article-title":"Microscopic wire guide-based orotracheal mouse intubation: description, evaluation and comparison with transillumination.","volume":"42","author":"J Hamacher","year":"2008","journal-title":"Lab Anim"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1258\/002367707779399400","article-title":"A novel and simple method for endotracheal intubation of mice.","volume":"41","author":"EN Spoelstra","year":"2007","journal-title":"Lab Anim"},{"key":"ref4","first-page":"52","article-title":"A novel method for endotracheal intubation of mice and rats used in imaging studies.","volume":"44","author":"B Rivera","year":"2005","journal-title":"Contemp Top Lab Anim Sci"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1258\/002367703766453047","article-title":"Video-assisted orotracheal intubation in mice.","volume":"37","author":"A Vergari","year":"2003","journal-title":"Lab Anim"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1139\/m77-120","article-title":"Experimental pulmonary infection of mice by tracheal intubation of Pseudomonas aeruginosa: the use of antineoplastic agents to overcome natural resistance.","volume":"23","author":"LB Schook","year":"1977","journal-title":"Can J Microbiol"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1080\/01902140490476382","article-title":"Direct visual instillation as a method for efficient delivery of fluid into the distal airspaces of anesthetized mice.","volume":"30","author":"X Su","year":"2004","journal-title":"Exp Lung Res"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1089\/jamp.2008.0721","article-title":"Analgesic effect from Ibuprofen nanoparticles inhaled by male mice.","volume":"22","author":"AA Onischuk","year":"2009","journal-title":"J Aerosol Med Pulm Drug Deliv"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"6437","DOI":"10.1128\/AEM.01194-08","article-title":"Characterization and deposition of respirable large- and small-particle bioaerosols.","volume":"74","author":"RJ Thomas","year":"2008","journal-title":"Appl Environ Microbiol"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"1765","DOI":"10.1007\/s11095-006-9029-y","article-title":"Applicability of an ultrasonic nebulization system for the airways delivery of beclomethasone dipropionate in a murine model of asthma.","volume":"23","author":"B Hrvacic","year":"2006","journal-title":"Pharm Res"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"1001","DOI":"10.1093\/jac\/34.6.1001","article-title":"Prophylactic efficacy of aerosolized liposomal (AmBisome) and non-liposomal (Fungizone) amphotericin B in murine pulmonary aspergillosis.","volume":"34","author":"SD Allen","year":"1994","journal-title":"J Antimicrob Chemother"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"702","DOI":"10.1001\/archinte.1961.03620050068007","article-title":"Tularemia vaccine study. II. Respiratory challenge.","volume":"107","author":"S Saslaw","year":"1961","journal-title":"Archives of internal medicine"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1001\/archinte.1961.03620050055006","article-title":"Tularemia vaccine study. I. Intracutaneous challenge.","volume":"107","author":"S Saslaw","year":"1961","journal-title":"Archives of internal medicine"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"2763","DOI":"10.1001\/jama.285.21.2763","article-title":"Tularemia as a biological weapon: medical and public health management.","volume":"285","author":"DT Dennis","year":"2001","journal-title":"JAMA : the journal of the American Medical Association"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"8345","DOI":"10.1128\/IAI.73.12.8345-8352.2005","article-title":"A mutant of Francisella tularensis strain SCHU S4 lacking the ability to express a 58-kilodalton protein is attenuated for virulence and is an effective live vaccine.","volume":"73","author":"S Twine","year":"2005","journal-title":"Infection and immunity"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"415","DOI":"10.4049\/jimmunol.87.4.415","article-title":"Prophylactic effectiveness of live and killed tularemia vaccines. I. Production of vaccine and evaluation in the white mouse and guinea pig.","volume":"87","author":"HT Eigelsbach","year":"1961","journal-title":"Journal of immunology"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/S1286-4579(02)00084-9","article-title":"Innate and adaptive immune responses to an intracellular bacterium, Francisella tularensis live vaccine strain.","volume":"5","author":"KL Elkins","year":"2003","journal-title":"Microbes and infection\/Institut Pasteur"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1016\/j.plasmid.2010.07.001","article-title":"Construction of a bioluminescence reporter plasmid for Francisella tularensis.","volume":"64","author":"XR Bina","year":"2010","journal-title":"Plasmid"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1080\/089583799197131","article-title":"The importance of the diluent for airway transport of toluene diisocyanate following intranasal dosing of mice.","volume":"11","author":"K Ebino","year":"1999","journal-title":"Inhal Toxicol"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"L833","DOI":"10.1152\/ajplung.00173.2001","article-title":"Distribution of intranasal instillations in mice: effects of volume, time, body position, and anesthesia.","volume":"282","author":"DS Southam","year":"2002","journal-title":"Am J Physiol Lung Cell Mol Physiol"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"1344","DOI":"10.1016\/S0264-410X(99)00401-6","article-title":"Cytokine mRNAs in the nasal-associated lymphoid tissue during influenza virus infection and nasal vaccination.","volume":"18","author":"K Matsuo","year":"2000","journal-title":"Vaccine"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"1707","DOI":"10.1172\/JCI6175","article-title":"Short ragweed allergen induces eosinophilic lung disease in HLA-DQ transgenic mice.","volume":"103","author":"SP Chapoval","year":"1999","journal-title":"J Clin Invest"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"3209","DOI":"10.1016\/S0264-410X(02)00247-5","article-title":"Tracking the tissue distribution of marker dye following intranasal delivery in mice and chinchillas: a multifactorial analysis of parameters affecting nasal retention.","volume":"20","author":"A Visweswaraiah","year":"2002","journal-title":"Vaccine"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1211\/0022357011775929","article-title":"Tissue distribution of radioactivity following intranasal administration of radioactive microspheres.","volume":"53","author":"JE Eyles","year":"2001","journal-title":"J Pharm Pharmacol"},{"key":"ref25","article-title":"Laboratory Animal Anesthesia","author":"P Flecknell","year":"2009"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"3909","DOI":"10.1128\/JVI.02012-09","article-title":"A mouse model of lethal infection for evaluating prophylactics and therapeutics against Monkeypox virus.","volume":"84","author":"J Stabenow","year":"2010","journal-title":"Journal of virology"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1016\/j.ijantimicag.2007.11.013","article-title":"Differential pathogenesis of cowpox virus intranasal infections in mice induced by low and high inoculum volumes and effects of cidofovir treatment.","volume":"31","author":"DF Smee","year":"2008","journal-title":"Int J Antimicrob Agents"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"877","DOI":"10.1016\/j.bbrc.2010.12.078","article-title":"Specific inhibition of nitric oxide synthases at different time points in a murine model of pulmonary sepsis.","volume":"404","author":"M Lange","year":"2011","journal-title":"Biochemical and biophysical research communications"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"2133","DOI":"10.1099\/mic.0.048157-0","article-title":"Stenotrophomonas maltophilia strains replicate and persist in the murine lung, but to significantly different degrees.","volume":"157","author":"R Rouf","year":"2011","journal-title":"Microbiology"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1186\/1471-2180-11-71","article-title":"The role of reactive oxygen intermediates in experimental coccidioidomycois in mice.","volume":"11","author":"DA Margolis","year":"2011","journal-title":"BMC microbiology"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"e14088","DOI":"10.1371\/journal.pone.0014088","article-title":"Attenuated response of aged mice to respiratory Francisella novicida is characterized by reduced cell death and absence of subsequent hypercytokinemia.","volume":"5","author":"CA Mares","year":"2010","journal-title":"PLoS One"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"1289","DOI":"10.1128\/IAI.01139-10","article-title":"Mucosal vaccination with a multivalent, live-attenuated vaccine induces multifactorial immunity against Pseudomonas aeruginosa acute lung infection.","volume":"79","author":"A Kamei","year":"2011","journal-title":"Infection and immunity"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"e20846","DOI":"10.1371\/journal.pone.0020846","article-title":"Enhanced virulence of Chlamydia muridarum respiratory infections in the absence of TLR2 activation.","volume":"6","author":"X He","year":"2011","journal-title":"PLoS One"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1016\/j.ijmm.2010.08.020","article-title":"Chlamydophila pneumoniae induces production of the defensin-like MIG\/CXCL9, which has in vitro antichlamydial activity.","volume":"301","author":"EP Balogh","year":"2011","journal-title":"International journal of medical microbiology : IJMM"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"e22663","DOI":"10.1371\/journal.pone.0022663","article-title":"Outer Membrane Protein A (OmpA) of Shigella flexneri 2a, Induces Protective Immune Response in a Mouse Model.","volume":"6","author":"D Pore","year":"2011","journal-title":"PLoS One"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"1754","DOI":"10.1016\/j.vaccine.2010.12.117","article-title":"Intranasal vaccination with pneumococcal surface protein A plus poly(I\u2236C) protects against secondary pneumococcal pneumonia in mice.","volume":"29","author":"H Ezoe","year":"2011","journal-title":"Vaccine"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"2749","DOI":"10.1016\/j.vaccine.2011.01.090","article-title":"Efficacy of vaccination strategies against intranasal challenge with Brucella abortus in BALB\/c mice.","volume":"29","author":"N Surendran","year":"2011","journal-title":"Vaccine"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"432","DOI":"10.4049\/jimmunol.1001963","article-title":"CD8+ cells enhance resistance to pulmonary serotype 3 Streptococcus pneumoniae infection in mice.","volume":"186","author":"SE Weber","year":"2011","journal-title":"Journal of immunology"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"1378","DOI":"10.1016\/j.intimp.2011.03.024","article-title":"c-di-GMP protects against intranasal Acinetobacter baumannii infection in mice by chemokine induction and enhanced neutrophil recruitment.","volume":"11","author":"L Zhao","year":"2011","journal-title":"International immunopharmacology"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1111\/j.1538-7836.2010.04089.x","article-title":"Recombinant human tissue factor pathway inhibitor exerts anticoagulant, anti-inflammatory and antimicrobial effects in murine pneumococcal pneumonia.","volume":"9","author":"FE Van Den Boogaard","year":"2011","journal-title":"Journal of thrombosis and haemostasis : JTH"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"e1001191","DOI":"10.1371\/journal.ppat.1001191","article-title":"Pneumolysin activates the NLRP3 inflammasome and promotes proinflammatory cytokines independently of TLR4.","volume":"6","author":"EA McNeela","year":"2010","journal-title":"PLoS pathogens"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"3328","DOI":"10.1128\/IAI.01133-10","article-title":"CXCR6 Is a Marker for Protective Antigen-Specific Cells in the Lungs after Intranasal Immunization against Mycobacterium tuberculosis.","volume":"79","author":"LN Lee","year":"2011","journal-title":"Infection and immunity"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"e18519","DOI":"10.1371\/journal.pone.0018519","article-title":"Kinase activity profiling of pneumococcal pneumonia.","volume":"6","author":"AJ Hoogendijk","year":"2011","journal-title":"PLoS One"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"e19016","DOI":"10.1371\/journal.pone.0019016","article-title":"Bordetella pertussis infection exacerbates influenza virus infection through pertussis toxin-mediated suppression of innate immunity.","volume":"6","author":"VI Ayala","year":"2011","journal-title":"PLoS One"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"1151","DOI":"10.4049\/jimmunol.1001751","article-title":"Lactobacillus-mediated priming of the respiratory mucosa protects against lethal pneumovirus infection.","volume":"186","author":"SJ Gabryszewski","year":"2011","journal-title":"Journal of immunology"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"5907","DOI":"10.4049\/jimmunol.1001533","article-title":"The integrins Mac-1 and alpha4beta1 perform crucial roles in neutrophil and T cell recruitment to lungs during Streptococcus pneumoniae infection.","volume":"186","author":"A Kadioglu","year":"2011","journal-title":"Journal of immunology"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1186\/1465-9921-12-12","article-title":"Overexpression of sICAM-1 in the alveolar epithelial space results in an exaggerated inflammatory response and early death in Gram negative pneumonia.","volume":"12","author":"MP Mendez","year":"2011","journal-title":"Respiratory research"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"1649","DOI":"10.1016\/j.vaccine.2010.12.068","article-title":"Outer membrane vesicles obtained from Bordetella pertussis Tohama expressing the lipid A deacylase PagL as a novel acellular vaccine candidate.","volume":"29","author":"CJ Asensio","year":"2011","journal-title":"Vaccine"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1016\/j.micinf.2011.01.010","article-title":"Role of neutrophils and NADPH phagocyte oxidase in host defense against respiratory infection with virulent Francisella tularensis in mice.","volume":"13","author":"R KuoLee","year":"2011","journal-title":"Microbes and infection\/Institut Pasteur"},{"key":"ref50","doi-asserted-by":"crossref","first-page":"7080","DOI":"10.4049\/jimmunol.1003687","article-title":"Airway epithelial MyD88 restores control of Pseudomonas aeruginosa murine infection via an IL-1-dependent pathway.","volume":"186","author":"LA Mijares","year":"2011","journal-title":"Journal of immunology"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"836","DOI":"10.1128\/AAC.00670-10","article-title":"Comparative effects of carbapenems on bacterial load and host immune response in a Klebsiella pneumoniae murine pneumonia model.","volume":"55","author":"JJ Hilliard","year":"2011","journal-title":"Antimicrobial agents and chemotherapy"},{"key":"ref52","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/j.micpath.2011.03.013","article-title":"Nitric oxide synthase activity has limited influence on the control of Coccidioides infection in mice.","volume":"51","author":"A Gonzalez","year":"2011","journal-title":"Microbial pathogenesis"},{"key":"ref53","doi-asserted-by":"crossref","first-page":"636","DOI":"10.1093\/infdis\/jir301","article-title":"Combining vaccination and postexposure CpG therapy provides optimal protection against lethal sepsis in a biodefense model of human melioidosis.","volume":"204","author":"A Easton","year":"2011","journal-title":"The Journal of infectious diseases"},{"key":"ref54","doi-asserted-by":"crossref","first-page":"2437","DOI":"10.1016\/j.vaccine.2011.01.031","article-title":"Induction of protection in mice against a respiratory challenge by a vaccine formulated with the Chlamydia major outer membrane protein adjuvanted with IC31(R).","volume":"29","author":"C Cheng","year":"2011","journal-title":"Vaccine"},{"key":"ref55","article-title":"The Mouse in Biomedical Research. 2nd ed","year":"2007"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0031359","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,6,20]],"date-time":"2023-06-20T17:10:31Z","timestamp":1687281031000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0031359"}},"subtitle":[],"editor":[{"given":"Deepak","family":"Kaushal","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2012,2,24]]},"references-count":55,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2012,2,24]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0031359","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,2,24]]}}}