{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T13:16:34Z","timestamp":1789996594879,"version":"4.0.1"},"reference-count":52,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2012,3,5]],"date-time":"2012-03-05T00:00:00Z","timestamp":1330905600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0031747","type":"journal-article","created":{"date-parts":[[2012,3,6]],"date-time":"2012-03-06T03:38:55Z","timestamp":1331005135000},"page":"e31747","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":22,"title":["Differential Expression of Iron Acquisition Genes by Brucella melitensis and Brucella canis during Macrophage Infection"],"prefix":"10.1371","volume":"7","author":[{"given":"Linda","family":"Eskra","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jill","family":"Covert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeremy","family":"Glasner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gary","family":"Splitter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2012,3,5]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"5248","DOI":"10.1128\/IAI.00460-07","article-title":"Brucella abortus requires the heme transporter BhuA for maintenance of chronic infection in BALB\/c mice.","volume":"75","author":"JT Paulley","year":"2007","journal-title":"Infect Immun"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"4496","DOI":"10.1128\/IAI.60.11.4496-4503.1992","article-title":"Identification of 2,3-dihydroxybenzoic acid as a Brucella abortus siderophore.","volume":"60","author":"I Lopez-Goni","year":"1992","journal-title":"Infect Immun"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1078\/1438-4221-00103","article-title":"Iron uptake mechanisms and their regulation in pathogenic bacteria.","volume":"291","author":"V Braun","year":"2001","journal-title":"Int J Med Microbiol"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1146\/annurev.micro.112408.134247","article-title":"TonB-dependent transporters: regulation, structure, and function.","volume":"64","author":"N Noinaj","year":"2010","journal-title":"Annu Rev Microbiol"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1111\/j.1600-065X.2010.00982.x","article-title":"An evolutionary strategy for a stealthy intracellular Brucella pathogen.","volume":"240","author":"A Martirosyan","year":"2011","journal-title":"Immunol Rev"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1111\/j.1462-5822.2005.00609.x","article-title":"Characterization of Brucella abortus lipopolysaccharide macrodomains as mega rafts.","volume":"8","author":"NFF Lapaque","year":"2006","journal-title":"Cell Microbiol"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"5040","DOI":"10.1128\/JB.186.15.5040-5051.2004","article-title":"Comparative whole-genome hybridization reveals genomic islands in Brucella species.","volume":"186","author":"G Rajashekara","year":"2004","journal-title":"J Bacteriol"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1046\/j.1462-5822.2002.00195.x","article-title":"Modulation of Brucella-induced macropinocytosis by lipid rafts mediates intracellular replication.","volume":"4","author":"MMS Watarai","year":"2002","journal-title":"Cell Microbiol"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1177\/030098589002700503","article-title":"Entry and intracellular localization of Brucella spp. in Vero cells: fluorescence and electron microscopy.","volume":"27","author":"PG Detilleux","year":"1990","journal-title":"Vet Pathol"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"1481","DOI":"10.1128\/IAI.71.3.1481-1490.2003","article-title":"Role of the Brucella suis lipopolysaccharide O antigen in phagosomal genesis and in inhibition of phagosome-lysosome fusion in murine macrophages.","volume":"71","author":"F Porte","year":"2003","journal-title":"Infect Immun"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"679","DOI":"10.1038\/76543","article-title":"Genome-directed primers for selective labeling of bacterial transcripts for DNA microarray analysis.","volume":"18","author":"AM Talaat","year":"2000","journal-title":"Nat Biotechnol"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"3899","DOI":"10.1002\/sim.1548","article-title":"On parametric empirical Bayes methods for comparing multiple groups using replicated gene expression profiles.","volume":"22","author":"C Kendziorski","year":"2003","journal-title":"Stat Med"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"D61","DOI":"10.1093\/nar\/gkl842","article-title":"NCBI reference sequences (RefSeq): a curated non-redundant sequence database of genomes, transcripts and proteins.","volume":"35","author":"KD Pruitt","year":"2007","journal-title":"Nucleic Acids Res"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1186\/1471-2105-4-41","article-title":"The COG database: an updated version includes eukaryotes.","volume":"4","author":"RL Tatusov","year":"2003","journal-title":"BMC Bioinformatics"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"D401","DOI":"10.1093\/nar\/gkl858","article-title":"PATRIC: the VBI PathoSystems Resource Integration Center.","volume":"35","author":"EE Snyder","year":"2007","journal-title":"Nucleic Acids Res"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"e45","DOI":"10.1093\/nar\/29.9.e45","article-title":"A new mathematical model for relative quantification in real-time RT-PCR.","volume":"29","author":"M Pfaffl","year":"2001","journal-title":"Nucleic Acids Res"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"7736","DOI":"10.1128\/IAI.69.12.7736-7742.2001","article-title":"Brucella abortus genes identified following constitutive growth and macrophage infection.","volume":"69","author":"L Eskra","year":"2001","journal-title":"Infect Immun"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"1045","DOI":"10.1189\/jlb.0103015","article-title":"Smooth and rough lipopolysaccharide phenotypes of Brucella induce different intracellular trafficking and cytokine\/chemokine release in human monocytes.","volume":"74","author":"MG Rittig","year":"2003","journal-title":"J Leukoc Biol"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1046\/j.1365-2249.2000.01295.x","article-title":"Growth of Brucella abortus in macrophages from resistant and susceptible mouse strains.","volume":"121","author":"J Sathiyaseelan","year":"2000","journal-title":"Clin Exp Immunol"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1002\/jlb.56.2.174","article-title":"Differentiated U937 cells exhibit increased bactericidal activity upon LPS activation and discriminate between virulent and avirulent Listeria and Brucella species.","volume":"56","author":"E Caron","year":"1994","journal-title":"J Leukoc Biol"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"1598","DOI":"10.1111\/j.1365-2958.2011.07668.x","article-title":"A two-component system, an anti-sigma factor and two paralogous ECF sigma factors are involved in the control of general stress response in Caulobacter crescentus.","volume":"80","author":"RF Louren\u00e7o","year":"2011","journal-title":"Mol Microbiol"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1111\/j.1365-2958.2010.07336.x","article-title":"General stress response in \u03b1-proteobacteria: PhyR and beyond.","volume":"78","author":"A Staron","year":"2010","journal-title":"Mol Microbiol"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1111\/j.1365-2958.2010.07323.x","article-title":"A structural model of anti-anti-\u03c3 inhibition by a two-component receiver domain: the PhyR stress response regulator.","volume":"78","author":"J Herrou","year":"2010","journal-title":"Mol Microbiol"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1099\/mic.0.042358-0","article-title":"Downregulation of Rv0189c, encoding a dihydroxyacid dehydratase, affects growth of Mycobacterium tuberculosis in vitro and in mice.","volume":"157","author":"V Singh","year":"2011","journal-title":"Microbiology"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"1731","DOI":"10.1021\/pr060135p","article-title":"Comparative proteome analysis of laboratory grown Brucella abortus 2308 and Brucella melitensis 16 M.","volume":"5","author":"M Eschenbrenner","year":"2006","journal-title":"J Proteome Res"},{"key":"ref26","doi-asserted-by":"crossref","DOI":"10.1016\/j.chom.2010.12.004","article-title":"Virulence of Mycobacterium tuberculosis depends on lipoamide dehydrogenase, a member of three multienzyme complexes.","volume":"9","author":"A Venugopal","year":"2011","journal-title":"Cell Host Microbe"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1016\/j.jnutbio.2005.03.019","article-title":"The Escherichia coli biotin regulatory system: a transcriptional switch.","volume":"16","author":"D Beckett","year":"2005","journal-title":"J Nutr Biochem"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"1094","DOI":"10.1111\/j.1365-2958.2006.05309.x","article-title":"A camel passes through the eye of a needle: protein unfolding activity of Clp ATPases.","volume":"61","author":"M Zolkiewski","year":"2006","journal-title":"Mol Microbiol"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"1631","DOI":"10.1128\/IAI.70.3.1631-1634.2002","article-title":"Induction of dnaK through its native heat shock promoter is necessary for intramacrophagic replication of Brucella suis.","volume":"70","author":"SEE K\u00f6hler","year":"2002","journal-title":"Infect Immun"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"1605","DOI":"10.1099\/00221287-146-7-1605","article-title":"Functional analysis of the ClpATPase ClpA of Brucella suis, and persistence of a knockout mutant in BALB\/c mice.","volume":"146","author":"E Ekaza","year":"2000","journal-title":"Microbiology"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"5911","DOI":"10.1128\/IAI.69.9.5911-5913.2001","article-title":"Brucella abortus HtrA functions as an authentic stress response protease but is not required for wild-type virulence in BALB\/c mice.","volume":"69","author":"RW Phillips","year":"2001","journal-title":"Infect Immun"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/S0378-1135(01)00380-7","article-title":"Re-examination of the role of the Brucella melitensis HtrA stress response protease in virulence in pregnant goats.","volume":"82","author":"RM Roop","year":"2001","journal-title":"Vet Microbiol"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"e18944","DOI":"10.1371\/journal.pone.0018944","article-title":"Factors Defining the Functional Oligomeric State of Escherichia coli DegP Protease.","volume":"6","author":"J Iwanczyk","year":"2011","journal-title":"PLoS One"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1128\/JCM.39.1.235-240.2001","article-title":"Limited genetic diversity of Brucella spp.","volume":"39","author":"B Gandara","year":"2001","journal-title":"J Clin Microbiol"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1099\/00207713-35-3-292","article-title":"Brucella, a monospecific genus as shown by deoxyribonucleic acid hybridization.","volume":"35","author":"JM Verger","year":"1985","journal-title":"Int J Syst Bacteriol"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"1717","DOI":"10.1086\/588383","article-title":"Serum-induced iron-acquisition systems and TonB contribute to virulence in <italic>Klebsiella pneumoniae<\/italic> causing primary pyogenic liver abscess.","volume":"197","author":"PF Hsieh","year":"2008","journal-title":"J Infect Dis"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1006\/mpat.2002.0500","article-title":"Brucella abortus siderophore 2,3-dihydroxybenzoic acid (DHBA) facilitates intracellular survival of the bacteria.","volume":"32","author":"MA Parent","year":"2002","journal-title":"Microb Pathog"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"1794","DOI":"10.1128\/IAI.71.4.1794-1803.2003","article-title":"Genetic organization and iron-responsive regulation of the Brucella abortus 2,3-dihydroxybenzoic acid biosynthesis operon, a cluster of genes required for wild-type virulence in pregnant cattle.","volume":"71","author":"BH Bellaire","year":"2003","journal-title":"Infect Immun"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1099\/00221287-148-2-353","article-title":"Brucella abortus strain 2308 produces brucebactin, a highly efficient catecholic siderophore.","volume":"148","author":"MI Gonzalez Carrero","year":"2002","journal-title":"Microbiology"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"2615","DOI":"10.1128\/IAI.67.5.2615-2618.1999","article-title":"The siderophore 2,3-dihydroxybenzoic acid is not required for virulence of Brucella abortus in BALB\/c mice.","volume":"67","author":"B Bellaire","year":"1999","journal-title":"Infect Immun"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1574-6968.2010.02186.x","article-title":"Effect of entF deletion on iron acquisition and erythritol metabolism by Brucella abortus 2308.","volume":"316","author":"N Jain","year":"2011","journal-title":"FEMS Microbiol Lett"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"2591","DOI":"10.1099\/mic.0.28782-0","article-title":"Irr regulates brucebactin and 2,3-dihydroxybenzoic acid biosynthesis, and is implicated in the oxidative stress resistance and intracellular survival of Brucella abortus.","volume":"152","author":"M Mart\u00ednez","year":"2006","journal-title":"Microbiology"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"3461","DOI":"10.1128\/JB.00317-11","article-title":"Anti-parallel and Interlinked Control of Cellular Iron Levels by the Irr and RirA Regulators of Agrobacterium tumefaciens.","volume":"193","author":"M Hibbing","year":"2011","journal-title":"J Bacteriol"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"4549","DOI":"10.1128\/IAI.64.11.4549-4556.1996","article-title":"Contribution of TonB- and Feo-mediated iron uptake to growth of Salmonella typhimurium in the mouse.","volume":"64","author":"RM Tsolis","year":"1996","journal-title":"Infect Immun"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"6329","DOI":"10.1128\/IAI.68.11.6329-6336.2000","article-title":"TonB is required for intracellular growth and virulence of Shigella dysenteriae.","volume":"68","author":"SA Reeves","year":"2000","journal-title":"Infect Immun"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"5783","DOI":"10.1128\/IAI.72.10.5783-5790.2004","article-title":"The Ton system, an ABC transporter, and a universally conserved GTPase are involved in iron utilization by Brucella melitensis 16 M.","volume":"72","author":"I Danese","year":"2004","journal-title":"Infect Immun"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1128\/JB.183.2.483-489.2001","article-title":"Transcriptional activation of Bordetella alcaligin siderophore genes requires the AlcR regulator with alcaligin as inducer.","volume":"183","author":"TJ Brickman","year":"2001","journal-title":"J Bacteriol"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"1838","DOI":"10.1128\/JB.01551-07","article-title":"The AraC-like transcriptional regulator DhbR is required for maximum expression of the 2,3-dihydroxybenzoic acid biosynthesis genes in Brucella abortus 2308 in response to iron deprivation.","volume":"190","author":"ES Anderson","year":"2008","journal-title":"J Bacteriol"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1186\/1471-2164-11-300","article-title":"Proteomics-based confirmation of protein expression and correction of annotation errors in the Brucella abortus genome.","volume":"11","author":"J Lamontagne","year":"2010","journal-title":"BMC Genomics"},{"key":"ref50","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1002\/med.10007","article-title":"Bacterial protease inhibitors.","volume":"22","author":"CT Supuran","year":"2002","journal-title":"Med Res Rev"},{"key":"ref51","article-title":"Bacterial resistance mechanisms against host defense peptides.","volume":"11","author":"T Koprivnjak","year":"2011","journal-title":"Cell Mol Life Sci May"},{"key":"ref52","doi-asserted-by":"crossref","first-page":"4912","DOI":"10.1128\/JB.00231-10","article-title":"Comparative study of the roles of AhpC and KatE as respiratory antioxidants in Brucella abortus 2308.","volume":"192","author":"KH Steele","year":"2010","journal-title":"J Bacteriol"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0031747","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,7,3]],"date-time":"2020-07-03T19:01:03Z","timestamp":1593802863000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0031747"}},"subtitle":[],"editor":[{"given":"Jean-Pierre","family":"Gorvel","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2012,3,5]]},"references-count":52,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2012,3,5]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0031747","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,3,5]]}}}