{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T13:09:08Z","timestamp":1791205748236,"version":"4.1.0"},"reference-count":40,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2012,3,7]],"date-time":"2012-03-07T00:00:00Z","timestamp":1331078400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0032857","type":"journal-article","created":{"date-parts":[[2012,3,7]],"date-time":"2012-03-07T22:02:29Z","timestamp":1331157749000},"page":"e32857","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":103,"title":["Reverse Genetics of SARS-Related Coronavirus Using Vaccinia Virus-Based Recombination"],"prefix":"10.1371","volume":"7","author":[{"given":"Sjoerd H. E.","family":"van den Worm","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Klara Kristin","family":"Eriksson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessika C.","family":"Zevenhoven","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Friedemann","family":"Weber","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roland","family":"Z\u00fcst","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Kuri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronald","family":"Dijkman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guohui","family":"Chang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stuart G.","family":"Siddell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric J.","family":"Snijder","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Volker","family":"Thiel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew D.","family":"Davidson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2012,3,7]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"1185","DOI":"10.1016\/j.meegid.2009.09.015","article-title":"Phylogenetic perspectives on the epidemiology and origins of SARS and SARS-like coronaviruses.","volume":"9","author":"CW Yip","year":"2009","journal-title":"Infect Genet Evol"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"1115","DOI":"10.1098\/rstb.2004.1491","article-title":"Management and prevention of SARS in China.","volume":"359","author":"N Zhong","year":"2004","journal-title":"Philos Trans R Soc Lond B Biol Sci"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1177\/135965350701200S01.1","article-title":"Human coronaviruses: what do they cause?","volume":"12","author":"L van der Hoek","year":"2007","journal-title":"Antivir Ther"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"S88","DOI":"10.1038\/nm1143","article-title":"Severe acute respiratory syndrome.","volume":"10","author":"JS Peiris","year":"2004","journal-title":"Nat Med"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1007\/978-1-59745-181-9_17","article-title":"Manipulation of the coronavirus genome using targeted RNA recombination with interspecies chimeric coronaviruses.","volume":"454","author":"CA de Haan","year":"2008","journal-title":"Methods Mol Biol"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1007\/978-1-59745-181-9_21","article-title":"Systematic assembly and genetic manipulation of the mouse hepatitis virus A59 genome.","volume":"454","author":"EF Donaldson","year":"2008","journal-title":"Methods Mol Biol"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1007\/978-1-59745-181-9_20","article-title":"Engineering infectious cDNAs of coronavirus as bacterial artificial chromosomes.","volume":"454","author":"F Almazan","year":"2008","journal-title":"Methods Mol Biol"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1186\/1743-422X-6-131","article-title":"Reverse genetic characterization of the natural genomic deletion in SARS-Coronavirus strain Frankfurt-1 open reading frame 7b reveals an attenuating function of the 7b protein in-vitro and in-vivo.","volume":"6","author":"S Pfefferle","year":"2009","journal-title":"Virol J"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1007\/978-1-59745-181-9_18","article-title":"Generation of recombinant coronaviruses using vaccinia virus as the cloning vector and stable cell lines containing coronaviral replicon RNAs.","volume":"454","author":"KK Eriksson","year":"2008","journal-title":"Methods Mol Biol"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"8692","DOI":"10.1128\/JVI.00527-07","article-title":"Interferon-mediated immunopathological events are associated with atypical innate and adaptive immune responses in patients with severe acute respiratory syndrome.","volume":"81","author":"MJ Cameron","year":"2007","journal-title":"J Virol"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1038\/nrmicro2147","article-title":"Coronaviruses post-SARS: update on replication and pathogenesis.","volume":"7","author":"S Perlman","year":"2009","journal-title":"Nat Rev Microbiol"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.cytogfr.2008.01.001","article-title":"Interferon and cytokine responses to SARS-coronavirus infection.","volume":"19","author":"V Thiel","year":"2008","journal-title":"Cytokine Growth Factor Rev"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"2366","DOI":"10.1182\/blood-2004-10-4166","article-title":"Chemokine up-regulation in SARS-coronavirus-infected, monocyte-derived human dendritic cells.","volume":"106","author":"HK Law","year":"2005","journal-title":"Blood"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"1953","DOI":"10.1099\/vir.0.81624-0","article-title":"Interaction of severe acute respiratory syndrome-associated coronavirus with dendritic cells.","volume":"87","author":"M Spiegel","year":"2006","journal-title":"J Gen Virol"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"3097","DOI":"10.1128\/JVI.79.5.3097-3106.2005","article-title":"Recombinant mouse hepatitis virus strain A59 from cloned, full-length cDNA replicates to high titers in vitro and is fully pathogenic in vivo.","volume":"79","author":"SE Coley","year":"2005","journal-title":"J Virol"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"5516","DOI":"10.1073\/pnas.97.10.5516","article-title":"Engineering the largest RNA virus genome as an infectious bacterial artificial chromosome.","volume":"97","author":"F Almazan","year":"2000","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"10600","DOI":"10.1128\/JVI.74.22.10600-10611.2000","article-title":"Strategy for systematic assembly of large RNA and DNA genomes: transmissible gastroenteritis virus model.","volume":"74","author":"B Yount","year":"2000","journal-title":"J Virol"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"866","DOI":"10.1177\/15353702-0322807-13","article-title":"The complete genome sequence of severe acute respiratory syndrome coronavirus strain HKU-39849 (HK-39).","volume":"228","author":"FY Zeng","year":"2003","journal-title":"Exp Biol Med (Maywood)"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"2305","DOI":"10.1099\/vir.0.19424-0","article-title":"Mechanisms and enzymes involved in SARS coronavirus genome expression.","volume":"84","author":"V Thiel","year":"2003","journal-title":"J Gen Virol"},{"key":"ref20","first-page":"229","article-title":"Development of mouse hepatitis virus and SARS-CoV infectious cDNA constructs.","volume":"287","author":"RS Baric","year":"2005","journal-title":"Curr Top Microbiol Immunol"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1007\/s12250-010-3124-2","article-title":"Establishment of the eukaryotic cell lines for inducible control of SARS-CoV nucleocapsid gene expression.","volume":"25","author":"GH Chang","year":"2010","journal-title":"Virol Sin"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1186\/1471-2172-10-35","article-title":"Toll-like receptors, chemokine receptors and death receptor ligands responses in SARS coronavirus infected human monocyte derived dendritic cells.","volume":"10","author":"HK Law","year":"2009","journal-title":"BMC Immunol"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"9790","DOI":"10.1128\/JVI.77.18.9790-9798.2003","article-title":"Multigene RNA vector based on coronavirus transcription.","volume":"77","author":"V Thiel","year":"2003","journal-title":"J Virol"},{"key":"ref24","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.00171-10","article-title":"Dendritic cell-specific antigen delivery by coronavirus vaccine vectors induces long-lasting protective antiviral and antitumor immunity.","volume":"1","author":"L Cervantes-Barragan","year":"2010","journal-title":"MBio"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"3391","DOI":"10.1128\/JVI.79.6.3391-3400.2005","article-title":"Single-amino-acid substitutions in open reading frame (ORF) 1b-nsp14 and ORF 2a proteins of the coronavirus mouse hepatitis virus are attenuating in mice.","volume":"79","author":"SM Sperry","year":"2005","journal-title":"J Virol"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.virusres.2007.03.025","article-title":"Animal models and vaccines for SARS-CoV infection.","volume":"133","author":"A Roberts","year":"2008","journal-title":"Virus Res"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/S0140-6736(03)13967-0","article-title":"Newly discovered coronavirus as the primary cause of severe acute respiratory syndrome.","volume":"362","author":"T Kuiken","year":"2003","journal-title":"Lancet"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1038\/423240a","article-title":"Aetiology: Koch's postulates fulfilled for SARS virus.","volume":"423","author":"RA Fouchier","year":"2003","journal-title":"Nature"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1038\/nm1001","article-title":"Pegylated interferon-alpha protects type 1 pneumocytes against SARS coronavirus infection in macaques.","volume":"10","author":"BL Haagmans","year":"2004","journal-title":"Nat Med"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"915","DOI":"10.1038\/425915a","article-title":"Virology: SARS virus infection of cats and ferrets.","volume":"425","author":"BE Martina","year":"2003","journal-title":"Nature"},{"key":"ref31","first-page":"3858","article-title":"Growth of human hepatoma cells lines with differentiated functions in chemically defined medium.","volume":"42","author":"H Nakabayashi","year":"1982","journal-title":"Cancer Res"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1159\/000131536","article-title":"Characterization of ouabain resistant, hypoxanthine phosphoribosyl transferase deficient human cells and their usefulness as a general method for the production of human cell hybrids.","volume":"28","author":"B Weissman","year":"1980","journal-title":"Cytogenet Cell Genet"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"1273","DOI":"10.1099\/0022-1317-82-6-1273","article-title":"Infectious RNA transcribed in vitro from a cDNA copy of the human coronavirus genome cloned in vaccinia virus.","volume":"82","author":"V Thiel","year":"2001","journal-title":"J Gen Virol"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1186\/1743-422X-3-63","article-title":"New low-viscosity overlay medium for viral plaque assays.","volume":"3","author":"M Matrosovich","year":"2006","journal-title":"Virol J"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/0378-1119(91)90366-J","article-title":"Construction of versatile low-copy-number vectors for cloning, sequencing and gene expression in Escherichia coli.","volume":"100","author":"RF Wang","year":"1991","journal-title":"Gene"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"1717","DOI":"10.1099\/vir.0.80044-0","article-title":"Rapid identification of coronavirus replicase inhibitors using a selectable replicon RNA.","volume":"85","author":"T Hertzig","year":"2004","journal-title":"J Gen Virol"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"522","DOI":"10.1016\/0042-6822(92)91246-Q","article-title":"Introduction of foreign DNA into the vaccinia virus genome by in vitro ligation: recombination-independent selectable cloning vectors.","volume":"190","author":"M Merchlinsky","year":"1992","journal-title":"Virology"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"5274","DOI":"10.1128\/JVI.73.7.5274-5281.1999","article-title":"Characterization of an equine arteritis virus replicase mutant defective in subgenomic mRNA synthesis.","volume":"73","author":"G van Marle","year":"1999","journal-title":"J Virol"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"6689","DOI":"10.1128\/JVI.72.8.6689-6698.1998","article-title":"ORF1a-encoded replicase subunits are involved in the membrane association of the arterivirus replication complex.","volume":"72","author":"Y van der Meer","year":"1998","journal-title":"J Virol"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"5927","DOI":"10.1128\/JVI.02501-05","article-title":"Ultrastructure and origin of membrane vesicles associated with the severe acute respiratory syndrome coronavirus replication complex.","volume":"80","author":"EJ Snijder","year":"2006","journal-title":"J Virol"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0032857","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,4,21]],"date-time":"2024-04-21T05:50:08Z","timestamp":1713678608000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0032857"}},"subtitle":[],"editor":[{"given":"Leo L. M.","family":"Poon","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2012,3,7]]},"references-count":40,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2012,3,7]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0032857","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,3,7]]}}}