{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T02:02:48Z","timestamp":1791165768201,"version":"4.1.0"},"reference-count":41,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2012,3,14]],"date-time":"2012-03-14T00:00:00Z","timestamp":1331683200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0033016","type":"journal-article","created":{"date-parts":[[2012,3,14]],"date-time":"2012-03-14T16:59:38Z","timestamp":1331744378000},"page":"e33016","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":31,"title":["Molecular Epidemiology of Neisseria meningitidis Serogroup B in Brazil"],"prefix":"10.1371","volume":"7","author":[{"given":"Ivano","family":"de Filippis","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Paula S.","family":"de Lemos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica B.","family":"Hostetler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kurt","family":"Wollenberg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudio T.","family":"Sacchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lee H.","family":"Harrison","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margaret C.","family":"Bash","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. Rebecca","family":"Prevots","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2012,3,14]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"1734","DOI":"10.1128\/JCM.30.7.1734-1738.1992","article-title":"Ongoing group B Neisseria meningitidis epidemic in S\u00e3o Paulo, Brazil, due to increased prevalence of a single clone of the ET-5 complex.","volume":"30","author":"CT Sacchi","year":"1992","journal-title":"Journal of Clinical Microbiology"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"1266","DOI":"10.1128\/JCM.02510-06","article-title":"Clonal distribution of invasive Neisseria meningitidis serogroup C strains circulating from 1976 to 2005 in greater S\u00e3o Paulo, Brazil.","volume":"45","author":"AP de Lemos","year":"2007","journal-title":"Journal of Clinical Microbiology"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"B3","DOI":"10.1016\/j.vaccine.2009.04.071","article-title":"Properties and clinical performance of vaccines containing outer membrane vesicles from Neisseria meningitidis.","volume":"27","author":"J Holst","year":"2009","journal-title":"Vaccine"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"1816","DOI":"10.1126\/science.287.5459.1816","article-title":"Identification of vaccine candidates against serogroup B meningococcus by whole-genome sequencing.","volume":"287","author":"M Pizza","year":"2000","journal-title":"Science"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"2088","DOI":"10.1128\/IAI.72.4.2088-2100.2004","article-title":"Vaccine potential of the Neisseria meningitidis 2086 lipoprotein.","volume":"72","author":"LD Fletcher","year":"2004","journal-title":"Infection and Immunity"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"10834","DOI":"10.1073\/pnas.0603940103","article-title":"A universal vaccine for serogroup B meningococcus.","volume":"103","author":"MM Giuliani","year":"2006","journal-title":"Proc Natl Acad Sci"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"1147","DOI":"10.1016\/j.trstmh.2007.06.012","article-title":"Hospital-based surveillance of meningococcal meningitis in Salvador, Brazil.","volume":"101","author":"SM Cordeiro","year":"2007","journal-title":"Transactions of the Royal Society of Tropical Medicine and Hygiene"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1111\/j.1365-3156.2007.01970.x","article-title":"Epidemiology of meningococcal disease in southern Brazil from 1995 to 2003, and molecular characterization of Neisseria meningitidis using multilocus sequence typing.","volume":"13","author":"LF Baethgen","year":"2008","journal-title":"Tropical Medicine & International Health"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/j.diagmicrobio.2005.06.015","article-title":"Multilocus sequence typing and repetitive element-based polymerase chain reaction analysis of Neisseria meningitides isolates in Brazil reveal the emergence of 11 new sequence types genetically related to the ST-32 and ST-41\/44 complexes and high prevalence of strains related to hypervirulent lineages.","volume":"53","author":"I de Filippis","year":"2005","journal-title":"Diagn Microbiol Infect Dis"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"2897","DOI":"10.1128\/JCM.39.8.2897-2903.2001","article-title":"Serosubtypes and PorA types of Neisseria meningitidis serogroup B isolated in Brazil during 1997\u20131998: overview and implications for vaccine development.","volume":"39","author":"CT Sacchi","year":"2001","journal-title":"Journal of clinical microbiology"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"1445","DOI":"10.1084\/jem.20020407","article-title":"NadA, a novel vaccine candidate of Neisseria meningitidis.","volume":"195","author":"M Comanducci","year":"2002","journal-title":"J Exp Med"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1084\/jem.20021911","article-title":"Vaccination against Neisseria meningitidis using three variants of the lipoprotein GNA1870.","volume":"197","author":"V Masignani","year":"2003","journal-title":"The Journal of Experimental Medicine"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"501","DOI":"10.4049\/jimmunol.177.1.501","article-title":"The meningococcal vaccine candidate GNA1870 binds the complement regulatory protein factor H and enhances serum resistance.","volume":"177","author":"G Madico","year":"2006","journal-title":"J Immunol"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"3140","DOI":"10.1073\/pnas.95.6.3140","article-title":"Multilocus sequence typing: a portable approach to the identification of clones within populations of pathogenic microorganisms.","volume":"95","author":"MC Maiden","year":"1998","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1111\/j.1365-2958.1994.tb01014.x","article-title":"The <italic>porA<\/italic> gene in serogroup A meningococci: evolutionary stability and mechanism of genetic variation.","volume":"12","author":"J Suker","year":"1994","journal-title":"Molecular microbiology"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1017\/S0950268898008656","article-title":"Amplification of the meningococcal <italic>porB<\/italic> gene for non-culture serotype characterization.","volume":"120","author":"R Urwin","year":"1998","journal-title":"Epidemiology and infection"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"1849","DOI":"10.1099\/mic.0.26131-0","article-title":"Antigenic diversity of meningococcal enterobactin receptor FetA, a vaccine component.","volume":"149","author":"EA Thompson","year":"2003","journal-title":"Microbiology"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1089\/gst.1995.1.9","article-title":"A new tool for assembling large sequencing projects.","volume":"1","author":"GG Sutton","year":"1995","journal-title":"Genome Science and Technology"},{"key":"ref19","first-page":"1","article-title":"Multiple sequence alignment using ClustalW and ClustalX.","volume":"2","author":"JD Thompson","year":"2002","journal-title":"Current protocols in bioinformatics Chapter"},{"key":"ref20","first-page":"95","article-title":"Bioedit: a user friendly biological sequence alignment editor and analysis program for Windows 95\/98\/NT.","volume":"41","author":"T Hall","year":"1999","journal-title":"Nucl Acids Symp Ser"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1186\/1471-2105-11-595","article-title":"BIGSdb: Scalable analysis of bacterial genome variation at the population level.","volume":"11","author":"KA Jolley","year":"2010","journal-title":"BMC bioinformatics"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1186\/1471-2105-5-113","article-title":"MUSCLE: a multiple sequence alignment method with reduced time and space complexity.","volume":"5","author":"RC Edgar","year":"2004","journal-title":"BMC bioinformatics"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"1251","DOI":"10.1534\/genetics.106.063305","article-title":"Inference of bacterial microevolution using multilocus sequence data.","volume":"175","author":"X Didelot","year":"2007","journal-title":"Genetics"},{"key":"ref24","article-title":"Garli website."},{"key":"ref25","article-title":"Phylogenetic analysis using parsimony (and other methods)","author":"D Swofford","year":"2003"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"4217","DOI":"10.1128\/IAI.72.7.4217-4223.2004","article-title":"NadA diversity and carriage in Neisseria meningitidis.","volume":"72","author":"M Comanducci","year":"2004","journal-title":"Infection and immunity"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1111\/j.1574-6976.2006.00057.x","article-title":"Molecular typing of meningococci: recommendations for target choice and nomenclature.","volume":"31","author":"KA Jolley","year":"2007","journal-title":"FEMS Microbiol"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1016\/S1413-8670(10)70051-9","article-title":"Microbiological epidemiological history of meningococcal disease in Rio de Janeiro, Brazil.","volume":"14","author":"DE Barroso","year":"2010","journal-title":"The Brazilian Journal of Infectious Diseases"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1128\/CDLI.5.3.348-354.1998","article-title":"Correlation between serological and sequencing analyses of the PorB outer membrane protein in the Neisseria meningitidis serotyping system.","volume":"5","author":"CT Sacchi","year":"1998","journal-title":"Clinical and diagnostic laboratory immunology"},{"key":"ref30","article-title":"Website for the Brazilian Institute of Geography and Statistics (IBGE)."},{"key":"ref31","doi-asserted-by":"crossref","first-page":"1170","DOI":"10.1099\/mic.0.2007\/014761-0","article-title":"Molecular epidemiology of meningococcal disease in England and Wales 1975\u20131995, before the introduction of serogroup C conjugate vaccines.","volume":"154","author":"JE Russell","year":"2008","journal-title":"Microbiology"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"5955","DOI":"10.1128\/IAI.72.10.5955-5962.2004","article-title":"Distribution of surface protein variants among hyperinvasive meningococci: implications for vaccine design.","volume":"72","author":"R Urwin","year":"2004","journal-title":"Infection and immunity"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"1127","DOI":"10.1086\/656741","article-title":"Multicenter, open-label, randomized phase II controlled trial of an investigational recombinant Meningococcal serogroup B vaccine with and without outer membrane vesicles, administered in infancy.","volume":"51","author":"J Findlow","year":"2010","journal-title":"Clinical Infectious Diseases"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"6086","DOI":"10.1016\/j.vaccine.2010.06.083","article-title":"Broad vaccine coverage predicted for a bivalent recombinant factor H binding protein based vaccine to prevent serogroup B meningococcal disease.","volume":"28","author":"HQ Jiang","year":"2010","journal-title":"Vaccine"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"1413","DOI":"10.1016\/j.vaccine.2010.12.039","article-title":"A phase 1 study of a meningococcal native outer membrane vesicle vaccine made from a group B strain with deleted lpxL1 and synX, over-expressed factor H binding protein, two PorAs and stabilized OpcA expression.","volume":"29","author":"PB Keiser","year":"2011","journal-title":"Vaccine"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"919","DOI":"10.1128\/CVI.00027-10","article-title":"Characterization of fHbp, nhba (gna2132), nadA, porA, and sequence type in group B meningococcal case isolates collected in England and Wales during January 2008 and potential coverage of an investigational group B meningococcal vaccine.","volume":"17","author":"J Lucidarme","year":"2010","journal-title":"Clinical and Vaccine Immunology"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"2794","DOI":"10.1016\/j.vaccine.2009.02.098","article-title":"Distribution and genetic variability of three vaccine components in a panel of strains representative of the diversity of serogroup B meningococcus.","volume":"27","author":"S Bambini","year":"2009","journal-title":"Vaccine"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1086\/600141","article-title":"Sequence diversity of the factor H binding protein vaccine candidate in epidemiologically relevant strains of serogroup B Neisseria meningitidis.","volume":"200","author":"E Murphy","year":"2009","journal-title":"The Journal of infectious diseases"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"4739","DOI":"10.1016\/j.vaccine.2011.04.092","article-title":"Prevalence and genetic diversity of candidate vaccine antigens among invasive Neisseria meningitidis isolates in the United States.","volume":"29","author":"X Wang","year":"2009","journal-title":"Vaccine"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"2122","DOI":"10.1016\/j.vaccine.2009.12.027","article-title":"Frequency of factor H-binding protein modular groups and susceptibility to cross-reactive bactericidal activity in invasive meningococcal isolates.","volume":"28","author":"R Pajon","year":"2010","journal-title":"Vaccine"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1111\/j.1574-6976.2006.00056.x","article-title":"Multilocus sequence typing for global surveillance of meningococcal disease.","volume":"31","author":"C Brehony","year":"2007","journal-title":"FEMS microbiology reviews"}],"updated-by":[{"DOI":"10.1371\/annotation\/6318254d-3f13-4cca-8bbe-08d0ff29aa64","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2012,7,10]],"date-time":"2012-07-10T00:00:00Z","timestamp":1341878400000}}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0033016","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,7,4]],"date-time":"2020-07-04T02:09:06Z","timestamp":1593828546000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0033016"}},"subtitle":[],"editor":[{"given":"Ray","family":"Borrow","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2012,3,14]]},"references-count":41,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2012,3,14]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0033016","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,3,14]]}}}