{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T19:52:16Z","timestamp":1770061936835,"version":"3.49.0"},"reference-count":30,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2012,3,12]],"date-time":"2012-03-12T00:00:00Z","timestamp":1331510400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0033530","type":"journal-article","created":{"date-parts":[[2012,3,12]],"date-time":"2012-03-12T21:31:58Z","timestamp":1331587918000},"page":"e33530","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":20,"title":["Minim Typing \u2013 A Rapid and Low Cost MLST Based Typing Tool for Klebsiella pneumoniae"],"prefix":"10.1371","volume":"7","author":[{"given":"Patiyan","family":"Andersson","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven Y. C.","family":"Tong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan M.","family":"Bell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John D.","family":"Turnidge","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philip M.","family":"Giffard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2012,3,12]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1146\/annurev.micro.59.030804.121325","article-title":"Multilocus sequence typing of bacteria.","volume":"60","author":"MC Maiden","year":"2006","journal-title":"Annu Rev Microbiol"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1586\/erm.09.84","article-title":"High-resolution DNA melting analysis in clinical research and diagnostics.","volume":"10","author":"JL Montgomery","year":"2010","journal-title":"Expert Rev Mol Diagn"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"597","DOI":"10.2217\/14622416.8.6.597","article-title":"High-resolution DNA melting analysis for simple and efficient molecular diagnostics.","volume":"8","author":"GH Reed","year":"2007","journal-title":"Pharmacogenomics"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"857","DOI":"10.1002\/humu.20951","article-title":"High-resolution DNA melting analysis: advancements and limitations.","volume":"30","author":"CT Wittwer","year":"2009","journal-title":"Hum Mutat"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"2679","DOI":"10.1128\/AAC.00063-09","article-title":"Analysis of bla(SHV) codon 238 and 240 allele mixtures using Sybr green high-resolution melting analysis.","volume":"53","author":"P Andersson","year":"2009","journal-title":"Antimicrob Agents Chemother"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"1378","DOI":"10.1128\/AAC.01476-09","article-title":"aac(6\u2032)-Ib-cr genotyping by simultaneous high-resolution melting analyses of an unlabeled probe and full-length amplicon.","volume":"54","author":"JM Bell","year":"2010","journal-title":"Antimicrob Agents Chemother"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"e19749","DOI":"10.1371\/journal.pone.0019749","article-title":"The Utility of High-Resolution Melting Analysis of SNP Nucleated PCR Amplicons-An MLST Based Staphylococcus aureus Typing Scheme.","volume":"6","author":"RA Lilliebridge","year":"2011","journal-title":"PLoS One"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1128\/AEM.01164-09","article-title":"Campylobacter jejuni and Campylobacter coli genotyping by high-resolution melting analysis of a flaA fragment.","volume":"76","author":"S Merchant-Patel","year":"2010","journal-title":"Appl Environ Microbiol"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"3431","DOI":"10.1128\/AEM.02702-06","article-title":"High-resolution DNA melt curve analysis of the clustered, regularly interspaced short-palindromic-repeat locus of Campylobacter jejuni.","volume":"73","author":"EP Price","year":"2007","journal-title":"Appl Environ Microbiol"},{"key":"ref10","article-title":"Preliminary validation of a novel high-resolution melt-based typing method based on the multilocus sequence typing scheme of Streptococcus pyogenes.","author":"LJ Richardson","year":"2010","journal-title":"Clin Microbiol Infect"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"432","DOI":"10.1373\/clinchem.2007.093658","article-title":"High-resolution melting analysis of the spa repeat region of Staphylococcus aureus.","volume":"54","author":"AJ Stephens","year":"2008","journal-title":"Clin Chem"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1016\/j.diagmicrobio.2010.03.015","article-title":"Rapid detection of H and R Panton-Valentine leukocidin isoforms in Staphylococcus aureus by high-resolution melting analysis.","volume":"67","author":"SY Tong","year":"2010","journal-title":"Diagn Microbiol Infect Dis"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"1126","DOI":"10.1111\/j.1469-0691.2009.02732.x","article-title":"High-resolution melting analysis of the spa locus reveals significant diversity within sequence type 93 methicillin-resistant Staphylococcus aureus from northern Australia.","volume":"15","author":"SY Tong","year":"2009","journal-title":"Clin Microbiol Infect"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"e29189","DOI":"10.1371\/journal.pone.0029189","article-title":"High-Resolution Melting Genotyping of Enterococcus faecium Based on Multilocus Sequence Typing Derived Single Nucleotide Polymorphisms.","volume":"6","author":"SY Tong","year":"2011","journal-title":"PLoS One"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"4178","DOI":"10.1128\/JCM.43.8.4178-4182.2005","article-title":"Multilocus sequence typing of Klebsiella pneumoniae nosocomial isolates.","volume":"43","author":"L Diancourt","year":"2005","journal-title":"J Clin Microbiol"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"1518","DOI":"10.1128\/JB.186.5.1518-1530.2004","article-title":"eBURST: inferring patterns of evolutionary descent among clusters of related bacterial genotypes from multilocus sequence typing data.","volume":"186","author":"EJ Feil","year":"2004","journal-title":"J Bacteriol"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1099\/jmm.0.05365-0","article-title":"Identification and interrogation of highly informative single nucleotide polymorphism sets defined by bacterial multilocus sequence typing databases.","volume":"53","author":"GA Robertson","year":"2004","journal-title":"J Med Microbiol"},{"key":"ref18","first-page":"365","article-title":"Primer3 on the WWW for general users and for biologist programmers.","volume":"132","author":"S Rozen","year":"2000","journal-title":"Methods Mol Biol"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"847","DOI":"10.1093\/bioinformatics\/16.9.847","article-title":"LIAN 3.0: detecting linkage disequilibrium in multilocus data. Linkage Analysis.","volume":"16","author":"B Haubold","year":"2000","journal-title":"Bioinformatics"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"2524","DOI":"10.1128\/JCM.02536-05","article-title":"Illustration of a common framework for relating multiple typing methods by application to macrolide-resistant Streptococcus pyogenes.","volume":"44","author":"JA Carrico","year":"2006","journal-title":"J Clin Microbiol"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"4190","DOI":"10.1128\/JCM.39.11.4190-4192.2001","article-title":"Determining confidence intervals when measuring genetic diversity and the discriminatory abilities of typing methods for microorganisms.","volume":"39","author":"H Grundmann","year":"2001","journal-title":"J Clin Microbiol"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"2465","DOI":"10.1128\/JCM.26.11.2465-2466.1988","article-title":"Numerical index of the discriminatory ability of typing systems: an application of Simpson's index of diversity.","volume":"26","author":"PR Hunter","year":"1988","journal-title":"J Clin Microbiol"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"1544","DOI":"10.1373\/clinchem.2007.088120","article-title":"Expanded instrument comparison of amplicon DNA melting analysis for mutation scanning and genotyping.","volume":"53","author":"MG Herrmann","year":"2007","journal-title":"Clin Chem"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"736","DOI":"10.1111\/j.1574-6976.2011.00268.x","article-title":"Multiresistant Gram-negative bacteria: the role of high-risk clones in the dissemination of antibiotic resistance.","volume":"35","author":"N Woodford","year":"2011","journal-title":"FEMS Microbiol Rev"},{"key":"ref25","article-title":"Fecal carriage of serotype K1 Klebsiella pneumoniae ST23 strains closely related to liver abscess isolates in Koreans living in Korea.","author":"DR Chung","year":"2011","journal-title":"Eur J Clin Microbiol Infect Dis Online"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"4061","DOI":"10.1128\/JCM.01577-08","article-title":"Evidence for clonal dissemination of the serotype K1 Klebsiella pneumoniae strain causing invasive liver abscesses in Korea.","volume":"46","author":"DR Chung","year":"2008","journal-title":"J Clin Microbiol"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"3761","DOI":"10.1128\/JCM.00977-11","article-title":"Molecular Typing and Virulence Analysis among Serotype K1 Klebsiella pneumoniae Isolated from Liver Abscess Patients and Stool Carriage from Non-Infectious Subjects in Hong Kong, Singapore and Taiwan.","volume":"49","author":"LK Siu","year":"2011","journal-title":"J Clin Microbiol"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1016\/j.jtbi.2005.08.035","article-title":"The impact of homologous recombination on the generation of diversity in bacteria.","volume":"239","author":"WP Hanage","year":"2006","journal-title":"J Theor Biol"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1186\/1471-2180-7-30","article-title":"Assessing the reliability of eBURST using simulated populations with known ancestry.","volume":"7","author":"KM Turner","year":"2007","journal-title":"BMC Microbiol"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"e4982","DOI":"10.1371\/journal.pone.0004982","article-title":"Virulent clones of Klebsiella pneumoniae: identification and evolutionary scenario based on genomic and phenotypic characterization.","volume":"4","author":"S Brisse","year":"2009","journal-title":"PLoS One"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0033530","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,7,4]],"date-time":"2020-07-04T03:10:44Z","timestamp":1593832244000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0033530"}},"subtitle":[],"editor":[{"given":"Igor","family":"Mokrousov","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2012,3,12]]},"references-count":30,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2012,3,12]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0033530","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,3,12]]}}}