{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T07:43:12Z","timestamp":1771659792757,"version":"3.50.1"},"reference-count":39,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2012,3,30]],"date-time":"2012-03-30T00:00:00Z","timestamp":1333065600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0034353","type":"journal-article","created":{"date-parts":[[2012,3,30]],"date-time":"2012-03-30T21:15:36Z","timestamp":1333142136000},"page":"e34353","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":51,"title":["High Viral Load of Human Bocavirus Correlates with Duration of Wheezing in Children with Severe Lower Respiratory Tract Infection"],"prefix":"10.1371","volume":"7","author":[{"given":"Yu","family":"Deng","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoyang","family":"Gu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaodong","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian","family":"Luo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhengxiu","family":"Luo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lijia","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhou","family":"Fu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiqiang","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Enmei","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2012,3,30]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"12891","DOI":"10.1073\/pnas.0504666102","article-title":"Cloning of a human parvovirus by molecular screening of respiratory tract samples.","volume":"102","author":"T Allander","year":"2005","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"904","DOI":"10.1086\/512196","article-title":"Human Bocavirus and Acute Wheezing in Children.","volume":"44","author":"T Allander","year":"2007","journal-title":"Clin Infect Dis"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"1316","DOI":"10.1164\/ajrccm.159.4.9807085","article-title":"Common respiratory viruses in lower airways of patients with acute hypersensitivity pneumonitis.","volume":"159","author":"A Dakhama","year":"1999","journal-title":"Am J Respir Crit Care Med"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1086\/506350","article-title":"The association of newly identified respiratory viruses with lower respiratory tract infections in Korean children, 2000\u20132005.","volume":"43","author":"EH Choi","year":"2006","journal-title":"Clin Infect Dis"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"1914","DOI":"10.1128\/JCM.34.8.1914-1917.1996","article-title":"Rapid diagnosis of human parainfluenza virus type 1 infection by quantitative reverse transcription-PCREnzyme hybridization assay.","volume":"34","author":"J Fan","year":"1996","journal-title":"J Clin Microbiol"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"848","DOI":"10.3201\/eid1205.051424","article-title":"Human Bocavirus infection, Canada.","volume":"12","author":"N Bastien","year":"2006","journal-title":"Emerg Infect Dis"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"1038","DOI":"10.1086\/512163","article-title":"Human bocavirus: a novel parvovirus epidemiologically associated with pneumonia requiring hospitalization in Thailand.","volume":"195","author":"AM Fry","year":"2007","journal-title":"J Infect Dis"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"1032","DOI":"10.1017\/S0950268808001842","article-title":"Human bocavirus respiratory infections in children.","volume":"137","author":"TB Gagliardi","year":"2009","journal-title":"Epidemiol Infect"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1128\/CMR.00030-07","article-title":"Human bocavirus: passenger or pathogen in acute respiratory tract infections?","volume":"21","author":"O Schildgen","year":"2008","journal-title":"Clin Microbiol Rev"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"862","DOI":"10.3201\/eid1205.051523","article-title":"Human bocavirus in children.","volume":"12","author":"V Foulongne","year":"2006","journal-title":"Emerg Infect Dis"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"1399","DOI":"10.1111\/j.1469-0691.2010.03147.x","article-title":"Impact of bacterial colonization on the severity and airway inflammation of children with virus induced wheezing.","volume":"6","author":"D Yu","year":"2010","journal-title":"Clin Microbiol Infect"},{"key":"ref12","article-title":"Manual of Clinical Microbiology, 6th edn","author":"PR Murray","year":"1980"},{"key":"ref13","first-page":"339","article-title":"Microscopic and bacteriologic analysis of expectoratedsputum.","volume":"506","author":"PR Murray","year":"1975","journal-title":"Mayo Clin Proc"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1002\/rmv.720","article-title":"Human bocavirus-the first 5 years.","volume":"22","author":"T Jartti","year":"2012","journal-title":"Rev Med Virol"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1016\/j.jcv.2009.11.015","article-title":"Correlation between bocavirus infection and humoral response, and co-infection with other respiratory viruses in children with acute respiratory infection.","volume":"47","author":"Kai Wanga","year":"2010","journal-title":"Journal of Clinical Virology"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1016\/j.ijporl.2011.10.025","article-title":"Human bocavirus in the nasopharynx of otitis-prone children.","volume":"76","author":"Liisa Lehtoranta","year":"2012","journal-title":"International Journal of Pediatric Otorhinolaryngology"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/j.jcv.2008.06.008","article-title":"Th1 and Th2 cytokine levels in nasopharyngeal aspirates from children with human bocavirus bronchiolitis.","volume":"43","author":"JY Chung","year":"2008","journal-title":"J Clin Virol"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1038\/89098","article-title":"A newly discovered human pneunurvirus isolated from young children with respiratory tract disease.","volume":"7","author":"BG Van den Hoogen","year":"2001","journal-title":"Nat Med"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"1232","DOI":"10.1002\/jmv.20689","article-title":"Frequent detection of human rhinoviruses, paramyxoviruses, coronaviruses, and bocavirus during acute respiratory tract infections.","volume":"78","author":"KE Arden","year":"2006","journal-title":"J Med Virol"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1016\/j.jcv.2010.07.016","article-title":"Human bocavirus in children: mono-detection, high viral load and viraemia are associated with respiratory tract infection.","volume":"49","author":"A Christensen","year":"2010","journal-title":"Journal of Clinical Virology"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"4131","DOI":"10.1128\/JCM.01508-09","article-title":"Detection of bocavirus in saliva of children with and without respiratory illness.","volume":"47","author":"ET Martin","year":"2009","journal-title":"Journal of Clinical Microbiology"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1086\/589244","article-title":"Human bocavirus in children hospitalized for acute gastroenteritis: a case-control study.","volume":"47","author":"WX Cheng","year":"2008","journal-title":"Clinical Infectious Diseases"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"897","DOI":"10.1097\/INF.0b013e3181757b16","article-title":"Clinical and epidemiologic characteristics of human bocavirus in Danish infants: results from a prospective birth cohort study.","volume":"27","author":"ML von Linstow","year":"2008","journal-title":"Pediatric Infectious Disease Journal"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"1625","DOI":"10.1086\/652405","article-title":"Frequent and prolonged shedding of bocavirus in young children attending daycare.","volume":"201","author":"ET Martin","year":"2010","journal-title":"Journal of Infectious Diseases"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"1276","DOI":"10.1086\/508213","article-title":"Human bocavirus infection in young children in the United States: molecular epidemiological profile and clinical characteristics of a newly emerging respiratory virus.","volume":"194","author":"D Kesebir","year":"2006","journal-title":"Journal of Infectious Diseases"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1016\/j.jcv.2007.01.008","article-title":"Human bocavirus in Italian patients with respiratory diseases.","volume":"38","author":"F Maggi","year":"2007","journal-title":"Journal of Clinical Virology"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"969","DOI":"10.1097\/INF.0b013e31817acfaa","article-title":"Human bocavirus infection in children with respiratory tract disease.","volume":"27","author":"N Brieu","year":"2008","journal-title":"Pediatric Infectious Disease Journal"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1097\/INF.0b013e3181626d2a","article-title":"Human bocavirus detection in nasopharyngeal aspirates of children without clinical symptoms of respiratory infection.","volume":"2008; 27","author":"ML Garc\u00eda-Garc\u00eda","year":"2008","journal-title":"Pediatric Infectious Disease Journal"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"217","DOI":"10.3201\/eid1402.070851","article-title":"Human bocavirus infections in hospitalized children and adults.","volume":"14","author":"J Longtin","year":"2008","journal-title":"Emerging Infectious Diseases"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"7739","DOI":"10.1128\/JVI.00614-09","article-title":"Human Bocavirus Can Be Cultured in Differentiated Human Airway Epithelial Cells.","volume":"83","author":"R Dijkman","year":"2009","journal-title":"J Virol"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"3015","DOI":"10.1099\/0022-1317-71-12-3015","article-title":"The two open reading frames of the 22K mRNA of human respiratory syncytial virus: sequence comparison of antigenic subgroups A and B and expression in vitro.","volume":"71","author":"PL Collins","year":"1990","journal-title":"J Gen Virol"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"484","DOI":"10.1128\/JCM.32.2.484-488.1994","article-title":"Rapid detection of parainfluenza virus type 3 RNA in respiratory specimens: use of reverse transcription-PCR-enzyme immunoassay.","volume":"32","author":"RA Karron","year":"1994","journal-title":"J Clin Microbiol"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"1132","DOI":"10.1128\/JCM.44.3.1132-1134.2006","article-title":"Detection of human bocavirus in Japanese children with lower respiratory tract infections.","volume":"44","author":"X Ma","year":"2006","journal-title":"J Clin Microbiol"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.jcv.2005.09.008","article-title":"Evidence of human coronavirus HKU1 and human bocavirus in Australian children.","volume":"35","author":"TP Sloots","year":"2006","journal-title":"J Clin Virol"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1086\/588674","article-title":"Seroepidemiology of human bocavirus defined using recombinant virus-like particles.","volume":"198","author":"JS Kahn","year":"2008","journal-title":"J Infect Dis"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1086\/505399","article-title":"Human bocavirus: prevalence and clinical spectrum at a children's hospital.","volume":"43","author":"JC Arnold","year":"2006","journal-title":"Clin Infect Dis"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1186\/1471-2334-6-109","article-title":"Frequent detection of bocavirus DNA in German children with respiratory tract infections.","volume":"6","author":"BB Weissbrich","year":"2006","journal-title":"BMC Infect Dis"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1002\/jmv.20815","article-title":"Human bocavirus in Iranian children with acute respiratory infections.","volume":"79","author":"M Naghipour","year":"2007","journal-title":"J Med Virol"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"1254","DOI":"10.3201\/eid1208.060261","article-title":"Bocavirus infection in hospitalized children, South Korea.","volume":"12","author":"JY Chung","year":"2006","journal-title":"Emerg Infect Dis"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0034353","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,7,5]],"date-time":"2020-07-05T03:35:09Z","timestamp":1593920109000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0034353"}},"subtitle":[],"editor":[{"given":"Malcolm Gracie","family":"Semple","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2012,3,30]]},"references-count":39,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2012,3,30]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0034353","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,3,30]]}}}