{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T04:39:51Z","timestamp":1782275991442,"version":"3.54.5"},"reference-count":49,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2012,5,15]],"date-time":"2012-05-15T00:00:00Z","timestamp":1337040000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0036872","type":"journal-article","created":{"date-parts":[[2012,5,15]],"date-time":"2012-05-15T21:15:07Z","timestamp":1337116507000},"page":"e36872","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":15,"title":["Enzymatic Analysis of Recombinant Japanese Encephalitis Virus NS2B(H)-NS3pro Protease with Fluorogenic Model Peptide Substrates"],"prefix":"10.1371","volume":"7","author":[{"given":"Muhammad","family":"Junaid","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chakard","family":"Chalayut","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Sehgelmeble Torrejon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chanan","family":"Angsuthanasombat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iryna","family":"Shutava","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maris","family":"Lapins","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jarl E. S.","family":"Wikberg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerd","family":"Katzenmeier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2012,5,15]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1136\/jnnp.68.4.405","article-title":"Japanese encephalitis.","volume":"68","author":"T Solomon","year":"2000","journal-title":"J Neurol Neurosurg Psychiatry"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1038\/nrmicro1067","article-title":"A structural perspective of the flavivirus life cycle.","volume":"3","author":"S Mukhopadhyay","year":"2005","journal-title":"Nat Rev Microbiol"},{"key":"ref3","first-page":"311","article-title":"Japanese encephalitis vaccines.","author":"SB Halstead","year":"2008"},{"key":"ref4","first-page":"63","article-title":"Japanese encephalitis.","author":"DS Burke","year":"1988"},{"key":"ref5","first-page":"1","article-title":"Centers for Disease Control and Prevention (CDC). Japanese encephalitis vaccines: recommendations of the Advisory Committee on Immunization Practices (ACIP).","volume":"RR-1","author":"M Fischer","year":"2010","journal-title":"MMWR Recomm Rep 59"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"533","DOI":"10.5694\/j.1326-5377.1999.tb127878.x","article-title":"Japanese encephalitis in north Queensland, Australia.","volume":"170","author":"JN Hanna","year":"1999","journal-title":"Med J Aust"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1016\/j.antiviral.2007.10.005","article-title":"Does antiviral therapy have a role in the control of Japanese encephalitis?","volume":"78","author":"EA Gould","year":"2008","journal-title":"Antiviral Research"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1016\/j.pneurobio.2010.01.008","article-title":"Overview: Japanese encephalitis.","volume":"91","author":"UK Misra","year":"2010","journal-title":"Progress in Neurobiology"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1517\/14712598.8.1.95","article-title":"Current use and development of vaccines for Japanese encephalitis.","volume":"8","author":"D Beasley","year":"2006","journal-title":"Expert opinion on Biological Therapy"},{"key":"ref10","first-page":"274","article-title":"Recent advances in Japanese encephalitits.","volume":"9","author":"T Solomon","year":"2003","journal-title":"Journal of Neuro Virology"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1016\/S1474-4422(08)70004-8","article-title":"New vaccines for Japanese encephalitis.","volume":"7","author":"T Solomon","year":"2008","journal-title":"The Lancet Neurology"},{"key":"ref12","first-page":"1","article-title":"Estimated global incidence of Japanese encephalitis: a systematic review.","volume":"BLT.10.085233","author":"GL Campbell","year":"2011","journal-title":"Bulletin of the World Health Organization"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1016\/j.antiviral.2008.08.004","article-title":"Molecular targets for flavivirus drug discovery.","volume":"81","author":"A Sampath","year":"2009","journal-title":"Antiviral Res"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"3149","DOI":"10.1016\/j.febslet.2010.05.062","article-title":"Biochemical characterisation of Murray Valley encephalitis virus proteinase.","volume":"584","author":"J Joy","year":"2010","journal-title":"FEBS Letters"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"606","DOI":"10.1016\/j.biocel.2006.10.025","article-title":"Characterization of the West Nile virus protease substrate specificity and inhibitors.","volume":"39","author":"NH Mueller","year":"2007","journal-title":"The International Journal of Biochemistry & Cell Biology"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.virusres.2006.01.021","article-title":"Functional characterization of the NS2B\/NS3 protease complex from seven viruses belonging to different groups inside the genus Flavivirus.","volume":"120","author":"M Bessaud","year":"2006","journal-title":"Virus Research"},{"key":"ref17","first-page":"991","article-title":"Flaviridae: the viruses and their replication.","author":"BD Lindenbach","year":"2001"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.virusres.2009.03.007","article-title":"Japanese encephalitis virus NS2B-NS3 protease induces caspase 3 activation and mitochondria-mediated apoptosis in human dulloblastoma cells.","volume":"143","author":"TC Yang","year":"2009","journal-title":"Virus Research"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"947","DOI":"10.1099\/0022-1317-79-5-947","article-title":"Virus-encoded proteinases of the Flaviviridae.","volume":"79","author":"MD Ryan","year":"1998","journal-title":"J Gen Virol"},{"key":"ref20","first-page":"10797","article-title":"Multiple full-length NS3 molecules are required for optimal unwinding of oligonucleotide DNA in vitro.","volume":"280","author":"AJ Tackett","year":"2005"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/j.virusres.2007.03.022","article-title":"Functional determinants of NS2B for activation of Japanese encephalitis virus NS3 protease.","volume":"127","author":"CW Lin","year":"2007","journal-title":"Virus Research"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1042\/BJ20051767","article-title":"Probing the substrate specificity of the dengue virus type 2 NS3 serine protease by using internally quenched fluorescent peptides.","volume":"397","author":"P Niyomrattanakit","year":"2006","journal-title":"The Biochemical Journal"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.jviromet.2003.09.001","article-title":"Activation of dengue protease autocleavage at the NS2B\u2013NS3 junction by recombinant NS3 and GST\u2013NS2B fusion proteins.","volume":"114","author":"CF Wu","year":"2003","journal-title":"Journal of Virological Methods"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"9963","DOI":"10.1074\/jbc.275.14.9963","article-title":"Purified NS2B\/NS3 serine protease of Dengue virus type 2 exhibits cofactor NS2B dependence for the cleavage of substrates with dibasic amino acids in vitro.","volume":"275","author":"R Yusof","year":"2000","journal-title":"J Biol Chem"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"45762","DOI":"10.1074\/jbc.M107360200","article-title":"Activity of recombinant dengue 2 virus NS3 protease in the presence of a truncated NS2B co-factor, small peptide substrates, and inhibitors.","volume":"276","author":"D Leung","year":"2001","journal-title":"J Biol Chem"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1110\/ps.072753207","article-title":"Structural evidence for regulation and specificity of flaviviral proteases and evolution of the Flaviviridae fold.","volume":"16","author":"AE Aleshin","year":"2007","journal-title":"Protein Sci"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1038\/nsmb1073","article-title":"Structural basis for the activation of flaviviral NS3 proteases from dengue and West Nile virus.","volume":"13","author":"P Erbel","year":"2006","journal-title":"Nat Struct Mol Biol"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1099\/0022-1317-76-3-573","article-title":"Processing of Japanese encephalitis virus non-structural proteins: NS2B-NS3 complex and heterologous protease.","volume":"76","author":"LR Jan","year":"1995","journal-title":"J Gen Virol"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"4501","DOI":"10.1128\/JVI.02719-06","article-title":"Switching the Substrate Specificity of the Two-Component NS2B-NS3 Flavivirus Proteinase by Structure-Based Mutagenesis.","volume":"81","author":"SA Shiryaev","year":"2007","journal-title":"Journal of Virology"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"13708","DOI":"10.1128\/JVI.78.24.13708-13716.2004","article-title":"Identification of residues in the dengue virus type 2 NS2B cofactor that are critical for NS3 protease activation.","volume":"78","author":"P Niyomrattanakit","year":"2004","journal-title":"J Virol"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1006\/abio.1998.3014","article-title":"Use of a fluorescence plate reader for measuring kinetic parameters with inner filter effect correction.","volume":"267","author":"Y Liu","year":"1999","journal-title":"Anal Biochem"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"D71","DOI":"10.1093\/nar\/gkr981","article-title":"Reorganizing the protein space at the Universal Protein Resource (UniProt).","volume":"40","author":"Consortium The UniProt","year":"2012","journal-title":"Nucleic Acids Res"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"779","DOI":"10.1006\/jmbi.1993.1626","article-title":"Comparative protein modelling by satisfaction of spatial restraints.","volume":"234","author":"A Sali","year":"1993","journal-title":"J Mol Biol"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"2223","DOI":"10.1099\/vir.0.82735-0","article-title":"Yellow fever virus NS3 protease: peptide-inhibition studies.","volume":"88","author":"K L\u00f6hr","year":"2007","journal-title":"J Gen Virol"},{"key":"ref35","first-page":"28766","article-title":"Functional profiling of recombinant NS3 proteases from all four serotypes of dengue virus using tetrapeptide and octapeptide substrate libraries J Biol Chem","volume":"280","author":"J Li","year":"2005"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"989","DOI":"10.1016\/j.bbagen.2008.03.018","article-title":"A comparative biochemical analysis of the NS2B(H) \u2013 NS3pro protease complex from four dengue virus serotypes.","volume":"1780","author":"T Iempridee","year":"2008","journal-title":"Biochimica et Biophysica Acta"},{"key":"ref37","first-page":"206","article-title":"In Vitro Determination of Dengue Virus Type 2 NS2B-NS3 Protease Activity with Fluorescent Peptide Substrates.","volume":"35","author":"R Khumthong","year":"2002","journal-title":"Journal of Biochemistry and Molecular Biology"},{"key":"ref38","first-page":"503","article-title":"Cleavage targets and the D-arginine-based inhibitors of the West Nile virus NS3 processing proteinase<bold>.<\/bold> Biochem J.","volume":"393","author":"SA Shiryaev","year":"2006"},{"key":"ref39","first-page":"283","article-title":"Homology modeling and molecular dynamics simulations of Dengue virus NS2B\/NS3 protease: insight into molecular interaction.","volume":"23","author":"A Wichapong","year":"2009","journal-title":"Journal of Molecular Recognition"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"2896","DOI":"10.1074\/jbc.M409931200","article-title":"Site-directed mutagenesis and kinetic studies of the West Nile Virus NS3 protease identify key enzyme-substrate interactions.","volume":"280","author":"KJ Chappell","year":"2005","journal-title":"J Biol Chem"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1146\/annurev.mi.44.100190.003245","article-title":"Flavivirus genome organization, expression and replication.","volume":"44","author":"TJ Chambers","year":"1990","journal-title":"Annu Rev Microbiol"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/j.abb.2006.11.005","article-title":"Substrate specificity of recombinant dengue 2 virus NS2B-NS3 protease: influence of natural and unnatural basic amino acids on hydrolysis of synthetic fluorescent substrates.","volume":"457","author":"IE Gouvea","year":"2007","journal-title":"Arch Biochem Biophys"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"38448","DOI":"10.1074\/jbc.M607641200","article-title":"Insights to substrate binding and processing by West Nile Virus NS3 protease through combined modeling, protease mutagenesis, and kinetic studies.","volume":"281","author":"KJ Chappell","year":"2006","journal-title":"J Biol Chem"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"28766","DOI":"10.1074\/jbc.M500588200","article-title":"Functional profiling of recombinant NS3 proteases from all four serotypes of dengue virus using tetrapeptide and octapeptide substrate libraries.","volume":"280","author":"J Li","year":"2005","journal-title":"J Biol Chem"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1186\/1423-0127-17-68","article-title":"Structure-guided mutagenesis of active site residues in the dengue virus two-component protease NS2B-NS3.","volume":"17","author":"W Salaemae","year":"2010","journal-title":"J Biomed Sci"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1016\/0042-6822(87)90144-9","article-title":"Complete nucleotide sequence of the Japanese encephalitis virus genome RNA.","volume":"161","author":"H Sumiyoshi","year":"1987","journal-title":"Virology"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"48535","DOI":"10.1074\/jbc.M406810200","article-title":"Enzymatic characterization and homology model of a catalytically active recombinant West Nile virus NS3 protease.","volume":"279","author":"TA Nall","year":"2004","journal-title":"J Biol Chem"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"1568","DOI":"10.1016\/j.jmb.2008.11.026","article-title":"Structure of West Nile virus NS3 protease: ligand stabilization of the catalytic conformation.","volume":"385","author":"G Robin","year":"2009","journal-title":"J Mol Biol"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1089\/adt.2007.101","article-title":"HTS identifies novel and specific uncompetitive inhibitors of the two-component NS2B-NS3 proteinase of West Nile virus.","volume":"5","author":"PA Johnston","year":"2007","journal-title":"Assay Drug Dev Technol"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0036872","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,7,7]],"date-time":"2020-07-07T18:45:44Z","timestamp":1594147544000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0036872"}},"subtitle":[],"editor":[{"given":"Luis","family":"Men\u00e9ndez-Arias","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2012,5,15]]},"references-count":49,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2012,5,15]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0036872","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,5,15]]}}}