{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T00:47:43Z","timestamp":1789692463256,"version":"4.0.1"},"reference-count":48,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2012,5,22]],"date-time":"2012-05-22T00:00:00Z","timestamp":1337644800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0037585","type":"journal-article","created":{"date-parts":[[2012,5,22]],"date-time":"2012-05-22T23:20:36Z","timestamp":1337728836000},"page":"e37585","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":34,"title":["Twelve Positions in a \u03b2-Lactamase That Can Expand Its Substrate Spectrum with a Single Amino Acid Substitution"],"prefix":"10.1371","volume":"7","author":[{"given":"Hyojeong","family":"Yi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kwang-Hwi","family":"Cho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun Sung","family":"Cho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karan","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William C.","family":"Nierman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heenam Stanley","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2012,5,22]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1146\/annurev-micro-090110-102911","article-title":"Epidemiological Expansion, Structural Studies, and Clinical Challenges of New \u03b2-Lactamases from Gram-Negative Bacteria.","volume":"65","author":"K Bush","year":"2011","journal-title":"Annual Review of Microbiology"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/S1473-3099(08)70041-0","article-title":"Extended-spectrum \u03b2-lactamase-producing <italic>Enterobacteriaceae<\/italic>: an emerging public-health concern.","volume":"8","author":"JDD Pitout","year":"2008","journal-title":"The Lancet Infectious Diseases"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1128\/CMR.00037-09","article-title":"Three Decades of beta-Lactamase Inhibitors.","volume":"23","author":"SM Drawz","year":"2010","journal-title":"Clin Microbiol Rev"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"1015","DOI":"10.1111\/j.1574-6976.2010.00222.x","article-title":"Natural evolution of TEM-1 beta-lactamase: experimental reconstruction and clinical relevance.","volume":"34","author":"ML Salverda","year":"2010","journal-title":"FEMS Microbiol Rev"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1093\/jac\/44.3.309","article-title":"Evolution and spread of SHV extended-spectrum beta-lactamases in gram-negative bacteria.","volume":"44","author":"J Heritage","year":"1999","journal-title":"J Antimicrob Chemother"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1128\/AAC.48.1.1-14.2004","article-title":"Growing Group of Extended-Spectrum \u03b2-Lactamases: the CTX-M Enzymes.","volume":"48","author":"R Bonnet","year":"2004","journal-title":"Antimicrob Agents Chemother"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1099\/00207713-48-1-317","article-title":"<italic>Burkholderia thailandensis<\/italic> sp. nov., a <italic>Burkholderia pseudomallei-<\/italic>like species.","volume":"1","author":"PJ Brett","year":"1998","journal-title":"Int J Syst Bacteriol 48 Pt"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1038\/nature10388","article-title":"Antibiotic resistance is ancient.","volume":"477","author":"VM D\/'Costa","year":"2011","journal-title":"Nature"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"876","DOI":"10.1128\/AAC.00946-08","article-title":"Naturally Occurring Class A beta-Lactamases from the <italic>Burkholderia cepacia<\/italic> Complex.","volume":"53","author":"L Poirel","year":"2009","journal-title":"Antimicrob Agents Chemother"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"1556","DOI":"10.1128\/JCM.01657-08","article-title":"Variations in ceftazidime and amoxicillin-clavulanate susceptibilities within a clonal infection of <italic>Burkholderia pseudomallei<\/italic>.","volume":"47","author":"IC Sam","year":"2009","journal-title":"J Clin Microbiol"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"2082","DOI":"10.1128\/AAC.47.7.2082-2087.2003","article-title":"<italic>Burkholderia pseudomallei<\/italic> class a beta-lactamase mutations that confer selective resistance against ceftazidime or clavulanic acid inhibition.","volume":"47","author":"C Tribuddharat","year":"2003","journal-title":"Antimicrob Agents Chemother"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1007\/s00253-010-2528-0","article-title":"Pathogenicity, virulence factors, and strategies to fight against <italic>Burkholderia cepacia<\/italic> complex pathogens and related species.","volume":"87","author":"JH Leitao","year":"2010","journal-title":"Appl Microbiol Biotechnol"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1586\/14787210.4.3.445","article-title":"Management of melioidosis.","volume":"4","author":"V Wuthiekanun","year":"2006","journal-title":"Expert Rev Anti Infect Ther"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1128\/CMR.18.2.383-416.2005","article-title":"Melioidosis: epidemiology, pathophysiology, and management.","volume":"18","author":"AC Cheng","year":"2005","journal-title":"Clin Microbiol Rev"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"e1000922","DOI":"10.1371\/journal.ppat.1000922","article-title":"The Early Stage of Bacterial Genome-Reductive Evolution in the Host.","volume":"6","author":"H Song","year":"2010","journal-title":"PLoS Pathog"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"e30789","DOI":"10.1371\/journal.pone.0030789","article-title":"Characterization of Ceftazidime Resistance Mechanisms in Clinical Isolates of <italic>Burkholderia pseudomallei<\/italic> from Australia.","volume":"7","author":"DS Sarovich","year":"2012","journal-title":"PLoS ONE"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1042\/bj2760269","article-title":"A standard numbering scheme for the class A beta-lactamases.","volume":"1)","author":"RP Ambler","year":"1991","journal-title":"Biochem J 276 (Pt"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1002\/prot.340160406","article-title":"Crystal structure of <italic>Escherichia coli<\/italic> TEM1 beta-lactamase at 1.8 A resolution.","volume":"16","author":"C Jelsch","year":"1993","journal-title":"Proteins"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1042\/bj3300581","article-title":"Catalytic properties of class A beta-lactamases: efficiency and diversity.","volume":"330","author":"A Matagne","year":"1998","journal-title":"Biochem J"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"6387","DOI":"10.1021\/bi200403e","article-title":"Resistance to the Third-Generation Cephalosporin Ceftazidime by a Deacylation-Deficient Mutant of the TEM \u03b2-Lactamase by the Uncommon Covalent-Trapping Mechanism.","volume":"50","author":"NT Antunes","year":"2011","journal-title":"Biochemistry"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"S19","DOI":"10.1093\/clinids\/24.Supplement_1.S19","article-title":"Evolution and Dissemination of \u03b2-Lactamases Accelerated by Generations of \u03b2-Lactam Antibiotics.","volume":"24","author":"AA Medeiros","year":"1997","journal-title":"Clinical Infectious Diseases"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"e1001115","DOI":"10.1371\/journal.pgen.1001115","article-title":"Evidence That Mutation Is Universally Biased towards AT in Bacteria.","volume":"6","author":"R Hershberg","year":"2010","journal-title":"PLoS Genet"},{"key":"ref23","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2011.00139","article-title":"Molecular investigations of PenA-mediated beta-lactam resistance in <italic>Burkholderia pseudomallei<\/italic>.","volume":"2","author":"DA Rholl","year":"2011","journal-title":"Frontiers in Microbiology"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"23052","DOI":"10.1074\/jbc.274.33.23052","article-title":"Effects on Substrate Profile by Mutational Substitutions at Positions 164 and 179 of the Class A TEMpUC19 \u03b2-Lactamase from Escherichia coli.","volume":"274","author":"SB Vakulenko","year":"1999","journal-title":"Journal of Biological Chemistry"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"600","DOI":"10.1128\/AAC.49.2.600-605.2005","article-title":"Novel SHV-Derived Extended-Spectrum \u03b2-Lactamase, SHV-57, That Confers Resistance to Ceftazidime but Not Cefazolin.","volume":"49","author":"L Ma","year":"2005","journal-title":"Antimicrob Agents Chemother"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1128\/AAC.50.2.731-738.2006","article-title":"Prediction of the Evolution of Ceftazidime Resistance in Extended-Spectrum \u03b2-Lactamase CTX-M-9.","volume":"50","author":"J Delmas","year":"2006","journal-title":"Antimicrob Agents Chemother"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"5423","DOI":"10.1021\/ja042850a","article-title":"Structure, function, and inhibition along the reaction coordinate of CTX-M beta-lactamases.","volume":"127","author":"Y Chen","year":"2005","journal-title":"Journal of the American Chemical Society"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1006\/jmbi.1994.1756","article-title":"TEM beta-Lactamase Mutants Hydrolysing Third-generation Cephalosporins : A Kinetic and Molecular Modelling Analysis.","volume":"244","author":"X Raquet","year":"1994","journal-title":"Journal of Molecular Biology"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"1878","DOI":"10.1128\/AAC.39.8.1878","article-title":"Effects of Asp-179 mutations in TEMpUC19 beta-lactamase on susceptibility to beta-lactams.","volume":"39","author":"SB Vakulenko","year":"1995","journal-title":"Antimicrob Agents Chemother"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"31771","DOI":"10.1074\/jbc.M110.198895","article-title":"Increased Structural Flexibility at the Active Site of a Fluorophore-conjugated \u03b2-Lactamase Distinctively Impacts Its Binding toward Diverse Cephalosporin Antibiotics.","volume":"286","author":"W-T Wong","year":"2011","journal-title":"Journal of Biological Chemistry"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1016\/j.jmb.2010.04.062","article-title":"Structural Insights into Substrate Recognition and Product Expulsion in CTX-M Enzymes.","volume":"400","author":"J Delmas","year":"2010","journal-title":"Journal of Molecular Biology"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/S0022-2836(02)00400-X","article-title":"Evolution of an Antibiotic Resistance Enzyme Constrained by Stability and Activity Trade-offs.","volume":"320","author":"X Wang","year":"2002","journal-title":"Journal of Molecular Biology"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1093\/jac\/dkf208","article-title":"Characterization of a laboratory-generated variant of BPS beta-lactamase from Burkholderia pseudomallei that hydrolyses ceftazidime.","volume":"50","author":"PL Ho","year":"2002","journal-title":"J Antimicrob Chemother"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"634","DOI":"10.1086\/338782","article-title":"Multiple mechanisms of antimicrobial resistance in Pseudomonas aeruginosa: our worst nightmare?","volume":"34","author":"DM Livermore","year":"2002","journal-title":"Clin Infect Dis"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1093\/jac\/24.3.295","article-title":"The antimicrobial susceptibility of <italic>Pseudomonas pseudomallei<\/italic>. Emergence of resistance in vitro and during treatment.","volume":"24","author":"DAB Dance","year":"1989","journal-title":"Journal of Antimicrobial Chemotherapy"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"1134","DOI":"10.1093\/jac\/dkh471","article-title":"Antibiotic susceptibility of 65 isolates of <italic>Burkholderia pseudomallei<\/italic> and <italic>Burkholderia mallei<\/italic> to 35 antimicrobial agents.","volume":"54","author":"FM Thibault","year":"2004","journal-title":"J Antimicrob Chemother"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1128\/AAC.43.2.213","article-title":"Comparative In Vitro Activities of Meropenem, Imipenem, Temocillin, Piperacillin, and Ceftazidime in Combination with Tobramycin, Rifampin, or Ciprofloxacin against <italic>Burkholderia cepacia<\/italic> Isolates from Patients with Cystic Fibrosis.","volume":"43","author":"S Bonacorsi","year":"1999","journal-title":"Antimicrob Agents Chemother"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1007\/s10156-002-0200-Z","article-title":"Antimicrobial resistance and DNA-fingerprinting pattern of <italic>Burkholderia cepacia<\/italic> blood isolates.","volume":"8","author":"T Saika","year":"2002","journal-title":"Journal of Infection and Chemotherapy"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1093\/jac\/dkh251","article-title":"Evolutionary mapping of the SHV beta-lactamase and evidence for two separate IS<italic>26<\/italic>-dependent <italic>bla<\/italic>SHV mobilization events from the <italic>Klebsiella pneumoniae<\/italic> chromosome.","volume":"54","author":"PJ Ford","year":"2004","journal-title":"J Antimicrob Chemother"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"3700","DOI":"10.1128\/JB.00067-08","article-title":"Opine-Based <italic>Agrobacterium<\/italic> Competitiveness: Dual Expression Control of the Agrocinopine Catabolism (<italic>acc<\/italic>) Operon by Agrocinopines and Phosphate Levels.","volume":"190","author":"HS Kim","year":"2008","journal-title":"J Bacteriol"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"2709","DOI":"10.1128\/JCM.30.10.2709-2713.1992","article-title":"Comparison of five methods, including the PDM Epsilometer test (E test), for antimicrobial susceptibility testing of <italic>Pseudomonas aeruginosa<\/italic>.","volume":"30","author":"LF Joyce","year":"1992","journal-title":"J Clin Microbiol"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1038\/nprot.2007.521","article-title":"Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances.","volume":"3","author":"I Wiegand","year":"2008","journal-title":"Nat Protocols"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/0378-1119(88)90117-5","article-title":"Improved broad-host-range plasmids for DNA cloning in Gram-negative bacteria.","volume":"70","author":"NT Keen","year":"1988","journal-title":"Gene"},{"key":"ref44","article-title":"Targeted mutagenesis of <italic>Burkholderia thailandensis<\/italic> and <italic>Burkholderia pseudomallei<\/italic> through natural transformation of PCR fragments.","author":"M Thongdee","year":"2008"},{"key":"ref45","article-title":"Vector plasmids for <italic>in vivo<\/italic> and <italic>in vitro<\/italic> manipulations of gram-negative bacteria.","author":"R Simon","year":"1983"},{"key":"ref46","article-title":"Molecular cloning : a laboratory manual, 2nd ed.","author":"J Sambrook","year":"1989"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"864","DOI":"10.1002\/jcc.21209","article-title":"Accelerating molecular dynamic simulation on graphics processing units.","volume":"30","author":"MS Friedrichs","year":"2009","journal-title":"Journal of Computational Chemistry"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"W116","DOI":"10.1093\/nar\/gkl282","article-title":"CASTp: computed atlas of surface topography of proteins with structural and topographical mapping of functionally annotated residues.","volume":"34","author":"J Dundas","year":"2006","journal-title":"Nucleic Acids Research"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0037585","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,7,8]],"date-time":"2020-07-08T00:15:40Z","timestamp":1594167340000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0037585"}},"subtitle":[],"editor":[{"given":"Renwick","family":"Dobson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2012,5,22]]},"references-count":48,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2012,5,22]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0037585","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,5,22]]}}}