{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,1,5]],"date-time":"2023-01-05T01:15:58Z","timestamp":1672881358669},"reference-count":51,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2012,6,20]],"date-time":"2012-06-20T00:00:00Z","timestamp":1340150400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0038715","type":"journal-article","created":{"date-parts":[[2012,6,21]],"date-time":"2012-06-21T12:12:38Z","timestamp":1340280758000},"page":"e38715","update-policy":"http:\/\/dx.doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":9,"title":["Basic Residues R260 and K357 Affect the Conformational Dynamics of the Major Facilitator Superfamily Multidrug Transporter LmrP"],"prefix":"10.1371","volume":"7","author":[{"given":"Wei","family":"Wang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hendrik W.","family":"van Veen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2012,6,20]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1128\/CMR.19.2.382-402.2006","article-title":"Clinically relevant chromosomally encoded multidrug resistance efflux pumps in bacteria.","volume":"19","author":"LJ Piddock","year":"2006","journal-title":"Clin Microbiol Rev"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1111\/j.1574-6976.2011.00290.x","article-title":"Broad-specificity efflux pumps and their role in multidrug resistance of Gram-negative bacteria.","volume":"36","author":"H Nikaido","year":"2012","journal-title":"FEMS Microbiol Rev"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1128\/JCM.00827-10","article-title":"Correlation of overexpression of efflux pump genes with antibiotic resistance in <italic>Escherichia coli<\/italic> strains clinically isolated from urinary tract infection patients.","volume":"49","author":"T Yasufuku","year":"2011","journal-title":"J Clin Microbiol"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1038\/nrc706","article-title":"Multidrug resistance in cancer: role of ATP-dependent transporters.","volume":"2","author":"MM Gottesman","year":"2002","journal-title":"Nat Rev Cancer"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1128\/MMBR.60.4.575-608.1996","article-title":"Proton-dependent multidrug efflux systems.","volume":"60","author":"IT Paulsen","year":"1996","journal-title":"Microbiol Rev"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1042\/bj20020957","article-title":"Structure and function of efflux pumps that confer resistance to drugs.","volume":"376","author":"MI Borges-Walmsley","year":"2003","journal-title":"Biochem J"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1093\/jac\/dki171","article-title":"Efflux-mediated antimicrobial resistance.","volume":"56","author":"K Poole","year":"2005","journal-title":"J Antimicrob Chemother"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1006\/scbi.1997.0064","article-title":"Multidrug transporters from bacteria to man: similarities in structure and function.","volume":"8","author":"HW van Veen","year":"1997","journal-title":"Semin Cancer Biol"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1006\/scdb.2000.0246","article-title":"Phylogeny of multidrug transporters.","volume":"12","author":"MH Saier Jr","year":"2001","journal-title":"Semin Cell Dev Biol"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"26092","DOI":"10.1074\/jbc.270.44.26092","article-title":"The lactococcal <italic>lmrP<\/italic> gene encodes a proton motive force-dependent drug transporter.","volume":"270","author":"H Bolhuis","year":"1995","journal-title":"J Biol Chem"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"24123","DOI":"10.1074\/jbc.271.39.24123","article-title":"Energetics and mechanism of drug transport mediated by the lactococcal multidrug transporter LmrP.","volume":"271","author":"H Bolhuis","year":"1996","journal-title":"J Biol Chem"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1159\/000099641","article-title":"Two proton translocation pathways in a secondary active multidrug transporter.","volume":"12","author":"A Bapna","year":"2007","journal-title":"J Mol Microbiol Biotechnol"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1074\/jbc.M306579200","article-title":"Facilitated drug influx by an energy-uncoupled secondary multidrug transporter.","volume":"279","author":"P Mazurkiewicz","year":"2004","journal-title":"J Biol Chem"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1146\/annurev.biophys.35.040405.102005","article-title":"Lessons from lactose permease.","volume":"35","author":"L Guan","year":"2006","journal-title":"Annu Rev Biophys Biomol Struct"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1038\/nature03978","article-title":"Crystal structure of a bacterial homologue of Na+\/Cl\u2013dependent neurotransmitter transporters.","volume":"437","author":"A Yamashita","year":"2005","journal-title":"Nature"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"470","DOI":"10.1126\/science.1186303","article-title":"Molecular basis of alternating access membrane transport by the sodium-hydantoin transporter Mhp1.","volume":"328","author":"T Shimamura","year":"2010","journal-title":"Science"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1038\/nature10737","article-title":"X-ray structures of LeuT in substrate-free outward-open and apo inward-open states.","volume":"481","author":"H Krishnamurthy","year":"2012","journal-title":"Nature"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"748","DOI":"10.1016\/j.bbapap.2008.12.018","article-title":"EmrE, a model for studying evolution and mechanism of ion-coupled transporters.","volume":"1794","author":"S Schuldiner","year":"2009","journal-title":"Biochim Biophys Acta"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"6966","DOI":"10.1074\/jbc.M808877200","article-title":"The secondary multidrug\/proton antiporter MdfA tolerates displacements of an essential negatively charged side chain.","volume":"284","author":"N Sigal","year":"2009","journal-title":"J Biol Chem"},{"key":"ref20","first-page":"163","article-title":"Staphylococcal multidrug efflux protein QacA.","volume":"3","author":"MH Brown","year":"2001","journal-title":"J Mol Microbiol Biotechnol"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"616","DOI":"10.1126\/science.1087619","article-title":"Structure and mechanism of the glycerol-3-phosphate transporter from <italic>Escherichia coli<\/italic>.","volume":"301","author":"Y Huang","year":"2003","journal-title":"Science"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"3653","DOI":"10.1096\/fj.10-156927","article-title":"A flexible cation binding site in the multidrug major facilitator superfamily transporter LmrP is associated with variable proton coupling.","volume":"24","author":"TA Schaedler","year":"2010","journal-title":"FASEB J"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"866","DOI":"10.1016\/j.bcp.2007.10.022","article-title":"On the energy-dependence of Hoechst 33342 transport by the ABC transporter LmrA.","volume":"75","author":"H Venter","year":"2008","journal-title":"Biochem Pharmacol"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"3662","DOI":"10.1128\/AEM.62.10.3662-3667.1996","article-title":"Controlled gene expression systems for <italic>Lactococcus lactis<\/italic> with the food-grade inducer nisin.","volume":"62","author":"PG de Ruyter","year":"1996","journal-title":"Appl Environ Microbiol"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1038\/341456a0","article-title":"Control of topology and mode of assembly of a polytopic membrane protein by positively charged residues.","volume":"341","author":"G von Heijne","year":"1989","journal-title":"Nature"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1146\/annurev.biochem.77.060806.091251","article-title":"Lipid-dependent membrane protein topogenesis.","volume":"78","author":"W Dowhan","year":"2009","journal-title":"Annu Rev Biochem"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"866","DOI":"10.1038\/nature02173","article-title":"An ABC transporter with a secondary-active multidrug translocator domain.","volume":"426","author":"H Venter","year":"2003","journal-title":"Nature"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"13900","DOI":"10.1021\/bi991262k","article-title":"The secondary multidrug transporter LmrP contains multiple drug interaction sites.","volume":"38","author":"M Putman","year":"1999","journal-title":"Biochemistry"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"7491","DOI":"10.1021\/bi0362018","article-title":"Characterization of YvcC (BmrA), a multidrug ABC transporter constitutively expressed in <italic>Bacillus subtilis<\/italic>.","volume":"43","author":"E Steinfels","year":"2004","journal-title":"Biochemistry"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"1370","DOI":"10.1128\/JB.184.5.1370-1377.2002","article-title":"NorA functions as a multidrug efflux protein in both cytoplasmic membrane vesicles and reconstituted proteoliposomes.","volume":"184","author":"JL Yu","year":"2002","journal-title":"J Bacteriol"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"16167","DOI":"10.1074\/jbc.270.27.16167","article-title":"Reconstitution of drug transport by purified P-glycoprotein.","volume":"270","author":"AB Shapiro","year":"1995","journal-title":"J Biol Chem"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"11273","DOI":"10.1074\/jbc.M308494200","article-title":"Reversible transport by the ATP-binding cassette multidrug export pump LmrA: ATP synthesis at the expense of downhill ethidium uptake.","volume":"279","author":"L Balakrishnan","year":"2004","journal-title":"J Biol Chem"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"1698","DOI":"10.1096\/fj.04-3558fje","article-title":"A critical role of a carboxylate in proton conduction by the ATP-binding cassette multidrug transporter LmrA.","volume":"19","author":"R Shilling","year":"2005","journal-title":"FASEB J"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"6363","DOI":"10.1128\/JB.187.18.6363-6369.2005","article-title":"Drug-lipid A interactions on the Escherichia coli ABC transporter MsbA.","volume":"187","author":"B Woebking","year":"2005","journal-title":"J Bacteriol"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"3774","DOI":"10.1073\/pnas.0800825105","article-title":"Opening and closing of the periplasmic gate in lactose permease.","volume":"105","author":"Y Zhou","year":"2008","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/j.jmb.2009.09.043","article-title":"Residues gating the periplasmic pathway of LacY.","volume":"394","author":"Y Zhou","year":"2009","journal-title":"J Mol Biol"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"610","DOI":"10.1126\/science.1088196","article-title":"Structure and mechanism of the lactose permease of <italic>Escherichia coli<\/italic>.","volume":"301","author":"J Abramson","year":"2003","journal-title":"Science"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"1713","DOI":"10.1038\/sj.emboj.7601618","article-title":"Structures and physiological roles of 13 integral lipids of bovine heart cytochrome c oxidase.","volume":"26","author":"K Shinzawa-Itoh","year":"2007","journal-title":"EMBO J"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"6591","DOI":"10.1093\/emboj\/20.23.6591","article-title":"Specific roles of protein-phospholipid interactions in the yeast cytochrome bc1 complex structure.","volume":"20","author":"C Lange","year":"2001","journal-title":"EMBO J"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"902","DOI":"10.1016\/j.jmb.2009.05.062","article-title":"A gating model for the archeal voltage-dependent K(+) channel KvAP in DPhPC and POPE:POPG decane lipid bilayers.","volume":"390","author":"D Schmidt","year":"2009","journal-title":"J Mol Biol"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1038\/nature05416","article-title":"Phospholipids and the origin of cationic gating charges in voltage sensors.","volume":"444","author":"D Schmidt","year":"2006","journal-title":"Nature"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1038\/nature06265","article-title":"Atomic structure of a voltage-dependent K+ channel in a lipid membrane-like environment.","volume":"450","author":"SB Long","year":"2007","journal-title":"Nature"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1126\/science.1213808","article-title":"Crystal structure of the human K2P TRAAK, a lipid- and mechano-sensitive K+ ion channel.","volume":"335","author":"SG Brohawn","year":"2012","journal-title":"Science"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"2646","DOI":"10.1007\/s00018-004-4298-2","article-title":"Proton motive force mediates a reorientation of the cytosolic domains of the multidrug transporter LmrP.","volume":"61","author":"B Gbaguidi","year":"2004","journal-title":"Cell Mol Life Sci"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"9369","DOI":"10.1074\/jbc.M708427200","article-title":"Interactions between phosphatidylethanolamine headgroup and LmrP, a multidrug transporter: a conserved mechanism for proton gradient sensing?","volume":"283","author":"P Hakizimana","year":"2008","journal-title":"J Biol Chem"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/0076-6879(71)22015-2","article-title":"Bacterial membranes.","volume":"22","author":"HR Kaback","year":"1971","journal-title":"Methods Enzymol"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"10904","DOI":"10.1021\/bi800778d","article-title":"Functional role of transmembrane helix 6 in drug binding and transport by the ABC transporter MsbA.","volume":"47","author":"B Woebking","year":"2008","journal-title":"Biochemistry"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1128\/AEM.62.1.178-183.1996","article-title":"A novel method for continuous determination of the intracellular pH in bacteria with the internally conjugated fluorescent probe 5 (and 6-)-carboxyfluorescein succinimidyl ester.","volume":"62","author":"P Breeuwer","year":"1996","journal-title":"Appl Environ Microbiol"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"5196","DOI":"10.1128\/JB.182.18.5196-5201.2000","article-title":"Cholate resistance in <italic>Lactococcus lactis<\/italic> is mediated by an ATP-dependent multispecific organic anion transporter.","volume":"182","author":"A Yokota","year":"2000","journal-title":"J Bacteriol"},{"key":"ref50","doi-asserted-by":"crossref","first-page":"e6137","DOI":"10.1371\/journal.pone.0006137","article-title":"A multidrug ABC transporter with a taste for salt.","volume":"4","author":"S Velamakanni","year":"2009","journal-title":"PLoS One"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"2867","DOI":"10.1002\/prot.22813","article-title":"Dissection of the conformational cycle of the multidrug\/lipidA ABC exporter MsbA.","volume":"78","author":"R Doshi","year":"2010","journal-title":"Proteins"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0038715","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,7,9]],"date-time":"2020-07-09T18:59:11Z","timestamp":1594321151000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0038715"}},"subtitle":[],"editor":[{"given":"Eugene A.","family":"Permyakov","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2012,6,20]]},"references-count":51,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2012,6,20]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0038715","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,6,20]]}}}