{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T16:40:06Z","timestamp":1791045606715,"version":"4.1.0"},"reference-count":40,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2012,12,6]],"date-time":"2012-12-06T00:00:00Z","timestamp":1354752000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0051200","type":"journal-article","created":{"date-parts":[[2012,12,6]],"date-time":"2012-12-06T21:50:02Z","timestamp":1354830602000},"page":"e51200","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":23,"title":["The Congenital Cataract-Linked A2V Mutation Impairs Tetramer Formation and Promotes Aggregation of \u03b2B2-Crystallin"],"prefix":"10.1371","volume":"7","author":[{"given":"Jia","family":"Xu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sha","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei-Jie","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Bo","family":"Xi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong-Bin","family":"Yan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ke","family":"Yao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2012,12,6]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/j.preteyeres.2005.10.001","article-title":"The lens of the eye as a focusing device and its response to stress","volume":"25","author":"A Banh","year":"2006","journal-title":"Prog Retin Eye Res"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1016\/j.pbiomolbio.2003.11.012","article-title":"Ageing and vision: structure, stability and function of lens crystallins","volume":"86","author":"H Bloemendal","year":"2004","journal-title":"Prog Biophys Mol Biol"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"10611","DOI":"10.1073\/pnas.1932958100","article-title":"Polydispersity of a mammalian chaperone: mass spectrometry reveals the population of oligomers in \u03b1B-crystallin","volume":"100","author":"JA Aquilina","year":"2003","journal-title":"Proc Natl Acad Sci USA"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/j.preteyeres.2006.10.003","article-title":"Crystallins in the eye: function and pathology","volume":"26","author":"UP Andley","year":"2007","journal-title":"Prog Retin Eye Res"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"1095","DOI":"10.1016\/j.bbagen.2009.05.008","article-title":"Lens aging: effcts of crystallins","volume":"1790","author":"KK Sharma","year":"2009","journal-title":"Biochim Biophys Acta"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/j.molmed.2012.03.005","article-title":"Protein misfolding and aggregation in cataract disease and prospects for prevention","volume":"18","author":"KL Moreau","year":"2012","journal-title":"Trends Mol Med"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"11250","DOI":"10.1074\/jbc.272.17.11250","article-title":"Size of human lens \u03b2-crystallin aggregates are distinguished by N-terminal truncation of \u03b2B1","volume":"272","author":"MS Ajaz","year":"1997","journal-title":"J Biol Chem"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1038\/eye.1999.113","article-title":"Structure of the crystallins","volume":"3b)","author":"C Slingsby","year":"1999","journal-title":"Eye (Lond) 13 (Pt"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"776","DOI":"10.1038\/347776a0","article-title":"X-ray analysis of <italic>\u03b2<\/italic>B2-crystallin and evolution of oligomeric lens proteins","volume":"347","author":"B Bax","year":"1990","journal-title":"Nature"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"1250","DOI":"10.1016\/0022-2836(94)90025-6","article-title":"Close packing of an oligomeric eye lens \u03b2-crystallin induces loss of symmetry and ordering of sequence extensions","volume":"236","author":"V Nalini","year":"1994","journal-title":"J Mol Biol"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"2606","DOI":"10.1110\/ps.03265903","article-title":"Crystal structure of truncated human \u03b2B1-crystallin","volume":"12","author":"RLM van Montfort","year":"2003","journal-title":"Protein Sci"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1021\/bi034617f","article-title":"Energetics of domain-domain interactions and entropy driven association of \u03b2-crystallins","volume":"43","author":"YV Sergeev","year":"2004","journal-title":"Biochemistry"},{"key":"ref13","doi-asserted-by":"crossref","unstructured":"Dolinska MB, Sergeev YV, Chan MP, Palmer I, Wingfield PT (2009) N-terminal extension of \u03b2B1-crystallin: identification of a critical region that modulates protein interaction with \u03b2A3-crystallin. Biochemistry.","DOI":"10.1021\/bi9013984"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"7179","DOI":"10.1021\/bi900313c","article-title":"Truncated human \u03b2B1-crystallin shows altered structural properties and interaction with human \u03b2A3-crystallin","volume":"48","author":"K Srivastava","year":"2009","journal-title":"Biochemistry"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1111\/j.1432-1033.1993.tb17765.x","article-title":"<sup>1<\/sup>H-NMR spectroscopy of bovine lens \u03b2B2-crystallin","volume":"213","author":"PG Cooper","year":"1993","journal-title":"Eur J Biochem"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"11062","DOI":"10.1021\/bi8012438","article-title":"Association properties of <italic>\u03b2<\/italic>B1- and <italic>\u03b2<\/italic>A3-crystallins: ability to form heterotetramers","volume":"47","author":"MP Chan","year":"2008","journal-title":"Biochemistry"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1006\/exer.1998.0481","article-title":"Age-related changes in human lens crystallins identified by two-dimensional electrophoresis and mass spectrometry","volume":"67","author":"KJ Lampi","year":"1998","journal-title":"Exp Eye Res"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1006\/exer.2001.1023","article-title":"Resistance of human \u03b2B2-crystallin to in vivo modification","volume":"73","author":"Z Zhang","year":"2001","journal-title":"Exp Eye Res"},{"key":"ref19","first-page":"144","article-title":"Characterization of a novel mutation in the <italic>CRYBB2<\/italic> gene associated with autosomal dominant congenital posterior subcapsular cataract in a Chinese family","volume":"17","author":"K Yao","year":"2011","journal-title":"Mol Vis"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"769","DOI":"10.1002\/humu.20724","article-title":"A novel mutation in <italic>AlphaA-crystallin (CRYAA)<\/italic> caused autosomal dominant congenital cataract in a large Chinese family","volume":"29","author":"F Gu","year":"2008","journal-title":"Hum Mutat"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/0378-1119(89)90358-2","article-title":"Site-directed mutagenesis by overlap extension using the polymerase chain reaction","volume":"77","author":"SN Ho","year":"1989","journal-title":"Gene"},{"key":"ref22","first-page":"177","article-title":"Site-directed mutagenesis using overlap extension PCR","volume":"57","author":"A Aiyar","year":"1996","journal-title":"Methods Mol Biol"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"948","DOI":"10.1016\/j.bbapap.2010.01.001","article-title":"Effects of congenital cataract mutation R116H on \u03b1A-crystallin structure, function and stability","volume":"1804","author":"M Pang","year":"2010","journal-title":"Biochim Biophys Acta - Proteins & Proteomics"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding","volume":"72","author":"MM Bradford","year":"1976","journal-title":"Anal Biochem"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1016\/0014-5793(76)80003-8","article-title":"Ultra-violet fluorescence of actin. Determination of native actin content in actin preparations","volume":"62","author":"KK Turoverov","year":"1976","journal-title":"FEBS Lett"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"e33700","DOI":"10.1371\/journal.pone.0033700","article-title":"A deadenylase assay by size-exclusion chromatography","volume":"7","author":"G-J He","year":"2012","journal-title":"PLoS ONE"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"e20564","DOI":"10.1371\/journal.pone.0020564","article-title":"The congenital cataract-linked G61C mutation destabilizes \u03b3D-crystallin and promotes non-native aggregation","volume":"6","author":"W Zhang","year":"2011","journal-title":"PLoS ONE"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"10451","DOI":"10.1021\/bi201375p","article-title":"The benefits of being <italic>\u03b2<\/italic>-crystallin heteromers: <italic>\u03b2<\/italic>B1-crystallin protects <italic>\u03b2<\/italic>A3-crystallin against aggregation during co-refolding","volume":"50","author":"S Wang","year":"2011","journal-title":"Biochemistry"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"1347","DOI":"10.1093\/protein\/10.11.1347","article-title":"Association properties of <italic>\u03b2<\/italic>B2- and <italic>\u03b2<\/italic>A3-crystallin: ability to form dimers","volume":"10","author":"JF Hejtmancik","year":"1997","journal-title":"Protein Eng"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"6592","DOI":"10.1021\/bi00480a007","article-title":"Quaternary interactions in eye lens <italic>\u03b2<\/italic>-crystallins: basic and acidic subunits of <italic>\u03b2<\/italic>-crystallins favor heterologous association","volume":"29","author":"C Slingsby","year":"1990","journal-title":"Biochemistry"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"33285","DOI":"10.1074\/jbc.M109.031344","article-title":"Hydrophobic core mutations associated with cataract development in mice destabilize human \u03b3D-crystallin","volume":"284","author":"KL Moreau","year":"2009","journal-title":"J Biol Chem"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"E1939","DOI":"10.1002\/humu.21386","article-title":"A novel <italic>CRYGD<\/italic> mutation (p.Trp43Arg) causing autosomal dominant congenital cataract in a Chinese family","volume":"32","author":"B Wang","year":"2011","journal-title":"Hum Mutat"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"E2050","DOI":"10.1002\/humu.21436","article-title":"A novel mutation in <italic>CRYBB1<\/italic> associated with congenital cataract-microcornea syndrome: the p.Ser129Arg mutation destabilizes the \u03b2B1\/\u03b2A3-crystallin heteromer but not the \u03b2B1-crystallin homomer","volume":"32","author":"KJ Wang","year":"2011","journal-title":"Hum Mutat"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1002\/humu.21648","article-title":"A novel mutation impairing the tertiary structure and stability of \u03b3C-crystallin (CRYGC) leads to cataract formation in humans and zebrafish lens","volume":"33","author":"X-Q Li","year":"2012","journal-title":"Hum Mutat"},{"key":"ref35","first-page":"241","article-title":"Deamidation alters interactions of \u03b2-crystallins in hetero-oligomers","volume":"15","author":"T Takata","year":"2009","journal-title":"Mol Vis"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1016\/j.exer.2004.06.011","article-title":"\u03b2-Crystallin association","volume":"79","author":"JF Hejtmancik","year":"2004","journal-title":"Exp Eye Res"},{"key":"ref37","first-page":"41","article-title":"Human beta-crystallin. I. Comparative studies on the beta 1, beta 2 and beta 3-crystallins","volume":"31","author":"JS Zigler Jr","year":"1980","journal-title":"Exp Eye Res"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"989","DOI":"10.1006\/jmbi.1999.2554","article-title":"Folding and self-assembly of the domains of \u03b2B2-crystallin from rat eye lens","volume":"286","author":"K Wieligmann","year":"1999","journal-title":"J Mol Biol"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/S1047-8477(02)00545-2","article-title":"Unfolding of human lens recombinant \u03b2B2- and \u03b3C-crystallins","volume":"139","author":"L Fu","year":"2002","journal-title":"J Struct Biol"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"1933","DOI":"10.1016\/j.febslet.2007.04.004","article-title":"Analysis of \u03b2B1-crystallin unfolding equilibrium by spin and fluorescence labeling: evidence of a dimeric intermediate","volume":"581","author":"HA Koteiche","year":"2007","journal-title":"FEBS Lett"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0051200","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,7,6]],"date-time":"2019-07-06T19:49:20Z","timestamp":1562442560000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0051200"}},"subtitle":[],"editor":[{"given":"Rizwan Hasan","family":"Khan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2012,12,6]]},"references-count":40,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2012,12,6]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0051200","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,12,6]]}}}