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Available: <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/www.rivm.nl\/dsresource?objectid=rivmp:181194&type=org&disposition=inline\" xlink:type=\"simple\">http:\/\/www.rivm.nl\/dsresource?objectid=rivmp:181194&amp;type=org&amp;disposition=inline<\/ext-link>. Accessed 2012 Sep 4."},{"key":"ref30","doi-asserted-by":"crossref","first-page":"3672","DOI":"10.1128\/AAC.00654-07","article-title":"Treatment of a clinically relevant plant-pathogenic fungus with an agricultural azole causes cross-resistance to medical azoles and potentiates caspofungin efficacy","volume":"51","author":"A Serfling","year":"2007","journal-title":"Antimicrob Agents Chemother"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"3014","DOI":"10.1128\/AAC.00459-07","article-title":"Cross-resistance to medical and agricultural azole drugs in yeasts from the oropharynx of human immunodeficiency virus patients and from environmental Bavarian vine grapes","volume":"51","author":"FM M\u00fcller","year":"2007","journal-title":"Antimicrob Agents Chemother"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1016\/S1473-3099(10)70264-4","article-title":"Population biological principles of drug-resistance evolution in infectious diseases","volume":"11","author":"PA zur Wiesch","year":"2011","journal-title":"Lancet Infect Dis"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"1305","DOI":"10.1111\/j.1365-294X.2012.05506.x","article-title":"Sex, drugs and recombination: the wild life of <italic>Aspergillus<\/italic>","volume":"21","author":"MC Fisher","year":"2012","journal-title":"Mol Ecol"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"1323","DOI":"10.1128\/AEM.61.4.1323-1330.1995","article-title":"Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes","volume":"61","author":"NL Glass","year":"1995","journal-title":"Appl Environ Microbiol"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1099\/ijs.0.63980-0","article-title":"Novel <italic>Neosartorya<\/italic> species isolated from soil in Korea","volume":"56","author":"SB Hong","year":"2006","journal-title":"Int J Syst Evol Microbiol"},{"key":"ref36","unstructured":"Clinical and Laboratory Standards Institute (2008) Reference method for broth dilution antifungal susceptibility testing of filamentous fungi; approved standard, 2nd ed. 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