{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T02:59:00Z","timestamp":1792119540738,"version":"4.4.0"},"reference-count":45,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2013,1,4]],"date-time":"2013-01-04T00:00:00Z","timestamp":1357257600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0053680","type":"journal-article","created":{"date-parts":[[2013,1,4]],"date-time":"2013-01-04T22:06:35Z","timestamp":1357337195000},"page":"e53680","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":25,"title":["Citrus Allergy from Pollen to Clinical Symptoms"],"prefix":"10.1371","volume":"8","author":[{"given":"Rosa Anna","family":"Iorio","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefano","family":"Del Duca","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisabetta","family":"Calamelli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiara","family":"Pula","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magda","family":"Lodolini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fortuna","family":"Scamardella","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Pession","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giampaolo","family":"Ricci","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2013,1,4]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1038\/sj.ejcn.1600974","article-title":"Quercetin intake and the incidence of cerebrovascular disease","volume":"54","author":"P Knekt","year":"2000","journal-title":"Eur J Clin Nutr"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1093\/jnci\/92.2.154","article-title":"Intake of flavonoids and lung cancer","volume":"92","author":"L Le Marchand","year":"2000","journal-title":"J Natl Cancer Inst"},{"key":"ref3","first-page":"95","article-title":"Urban air pollution and climate change as environmental risk factors of respiratory allergy: an update","volume":"20","author":"G D'Amato","year":"2010","journal-title":"J Investig Allergol Clin Immunol"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"717","DOI":"10.1016\/S0091-6749(99)70411-2","article-title":"Food allergy. Part 1: Immunopathogenesis and clinical disorders","volume":"103","author":"HA Sampson","year":"1999","journal-title":"J Allergy Clin Immun"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"805","DOI":"10.1016\/j.jaci.2004.03.014","article-title":"Update on food allergy","volume":"113","author":"HA Sampson","year":"2004","journal-title":"J Allergy Clin Immun"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"1080","DOI":"10.1016\/j.jaci.2005.07.004","article-title":"In vivo assessment with prick-to-prick testing and double-blind, placebo-controlled food challenge of allergenicity of apple cultivars","volume":"116","author":"ST Bolhaar","year":"2005","journal-title":"J Allergy Clin Immun"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"717","DOI":"10.1111\/j.1398-9995.2007.01425.x","article-title":"The prevalence, cost and basis of food allergy across Europe","volume":"62","author":"EN Mills","year":"2007","journal-title":"Allergy"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1016\/j.plantsci.2005.03.027","article-title":"Localisation and distribution of the major allergens in apple fruits","volume":"169","author":"G Marzban","year":"2005","journal-title":"Plant Sci"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1159\/000090384","article-title":"Orange Germin-Like Glycoprotein Cit s 1: An Equivocal Allergen","volume":"139","author":"O Ahrazem","year":"2006","journal-title":"Int Arch Allergy Immunol"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"1424","DOI":"10.1111\/j.1398-9995.2005.00903.x","article-title":"Isolation, cloning and allergenic reactivity of natural profilin Cit s 2, a major orange allergen","volume":"60","author":"G L\u00f2pez-Torrej\u00f2n","year":"2005","journal-title":"Allergy"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/0091-6749(86)90002-3","article-title":"A perspective on popular perceptions of adverse reactions to food","volume":"78","author":"AE Sloan","year":"1986","journal-title":"J Allergy Clin Immun"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1002\/mnfr.200500200","article-title":"Germin-like protein Cit s 1 and profilin Cit s 2 are major allergen in orange (<italic>Citrus sinensis<\/italic>) fruits","volume":"50","author":"JF Crespo","year":"2006","journal-title":"Mol Nutr Food Res"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1111\/j.1365-2222.1991.tb03196.x","article-title":"Evaluation of a multiple food specific IgE antibody test compared to parental perception, allergy skin tests and RAST","volume":"21","author":"BR Adler","year":"1991","journal-title":"Clin Exp Allergy"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1016\/0091-6749(89)90083-3","article-title":"Comparison of results of skin prick tests (with fresh foods and commercial food extracts) and RAST in 100 patients with oral allergy syndrome","volume":"83","author":"C Ortolani","year":"1989","journal-title":"J Allergy Clin Immunl"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/j.jaci.2003.10.059","article-title":"Differents patterns of allergen recognition in children allergic to orange","volume":"113","author":"MD Iba\u00f1ez","year":"2004","journal-title":"J Allergy Clin Immun"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"7239","DOI":"10.1021\/jf1006825","article-title":"Proteome from lemon fruit flavedo reveals that this tissue produces high amounts of the Cit s1 germin-like isoforms","volume":"58","author":"V Pignataro","year":"2010","journal-title":"J Agric Food Chem"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1007\/s12551-009-0010-y","article-title":"Structure and functions of profilins","volume":"1","author":"K Krishnan","year":"2009","journal-title":"Biophys Rev"},{"key":"ref18","unstructured":"Enrique E, Alonso R, Bartolome B, San Miguel-Moncin M, Bartra J, <etal>et al<\/etal>.. (2004) IgE reactivity to profilin in Platanus acerifolia pollen-sensitized subjects with plant-derived food allergy J Investig Allergol Clin Immunol 14: 335 \u2013 342."},{"key":"ref19","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1159\/000086332","article-title":"Lipid transfer proteins and allergy to oranges","volume":"137","author":"O Ahrazem","year":"2005","journal-title":"Int Arch Allergy Immunol"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1146\/annurev.arplant.47.1.627","article-title":"Lipid-transfer proteins in plants","volume":"47","author":"JC Kader","year":"1996","journal-title":"Annu Rev Plant Physiol Plant Mol Biol"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"993","DOI":"10.1016\/S0168-9452(02)00271-6","article-title":"Differential expression of two lipid transfer protein genes in reproductive organs of peach (Prunus persica L. Batsch)","volume":"163","author":"A Botton","year":"2002","journal-title":"Plant Sci"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"1007","DOI":"10.1007\/s00726-011-1043-7","article-title":"Simulated environmental criticalities affect transglutaminase of <italic>Malus<\/italic> and <italic>Corylus<\/italic> pollens having different allergenic potential","volume":"42","author":"RA Iorio","year":"2012","journal-title":"Amino Acids"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1159\/000053688","article-title":"Secretion of proinflammatory eicosanoid-like substances precedes allergen release from pollen grains in the initiation of allergic sensitization","volume":"124","author":"H Behrendt","year":"2001","journal-title":"Int Arch Allergy Immunol"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1159\/000144035","article-title":"Characterization of proteases, proteins, and eicosanoid-like substances in soluble extracts from allergenic pollen grains","volume":"147","author":"H Gunawan","year":"2008","journal-title":"Int Arch Allergy Immunol"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1104\/pp.68.3.727","article-title":"RNA, proteins and polyamines during tube growth in germinating apple pollen","volume":"68","author":"N Bagni","year":"1981","journal-title":"Plant Physiol"},{"key":"ref26","unstructured":"Wilkins TA, Smart LB (1996) Isolation of RNA from plant tissue \u2013 A laboratory guide to RNA: isolation, analysis, and synthesis. In: Krieg PA, editor. New York: Wiley-Liss. 21\u201342."},{"key":"ref27","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1007\/s11295-008-0177-6","article-title":"Expression profiling in <italic>HcrVf2<\/italic>-transformed apple plants in response to <italic>Venturia inaequalis<\/italic>","volume":"5","author":"R Paris","year":"2009","journal-title":"Tree Genetics & Genomes"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"RESEARCH0034","DOI":"10.1186\/gb-2002-3-7-research0034","article-title":"Accurate normalization of real-time quantitative RT-PCR by geometric averaging of multiple internal control genes","volume":"3","author":"J Vandesompele","year":"2002","journal-title":"Genome Biol"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"1831","DOI":"10.1007\/s11033-011-0925-9","article-title":"Selection of reference genes for quantitative real-time PCR analysis in citrus","volume":"39","author":"J Yan","year":"2012","journal-title":"Mol Biol Rep"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1016\/S0304-3940(02)01423-4","article-title":"Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data","volume":"339","author":"C Ramakers","year":"2003","journal-title":"Neurosci Lett"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2-\u0394\u0394CT method","volume":"25","author":"KJ Livak","year":"2001","journal-title":"Methods"},{"key":"ref32","first-page":"162","article-title":"Cross-reactivity Syndromes in Food Allergy","volume":"21","author":"BE Garcia","year":"2011","journal-title":"J Investig Allergol Clin Immunol"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1046\/j.1323-8930.2003.00303.x","article-title":"Self-reported hypersensitivity to exotic fruit in birch pollen-allergic patients","volume":"52","author":"NE Eriksson","year":"2003","journal-title":"Allergol Int"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1016\/S1081-1206(10)60720-X","article-title":"Anaphylaxis to lemon soap: citrus seed and peanut allergen cross-reactivity","volume":"98","author":"IN Glaspole","year":"2007","journal-title":"Ann Allergy Asthma Immunol"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1159\/000102612","article-title":"Anaphylaxis from mandarin (Citrus reticulata): identification of potential responsible allergens","volume":"144","author":"DG Ebo","year":"2007","journal-title":"Int Arch Allergy Immunol"},{"key":"ref36","first-page":"191","article-title":"Relevance of serum IgE estimation in allergic bronchial asthma with special reference to food allergy","volume":"24","author":"R Kumar","year":"2006","journal-title":"Asian Pac J Allergy Immunol"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"1033","DOI":"10.1111\/j.1365-2222.2008.02980.x","article-title":"Profilin sensitization detected in the office by skin prick test: a study of prevalence and clinical relevance of profilin as a plant food allergen","volume":"38","author":"R Asero","year":"2008","journal-title":"Clin Exp Allergy"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"1251","DOI":"10.1016\/j.molimm.2004.11.011","article-title":"Relationship between Bet v 1 and Bet v 2 specific IgE and food allergy in children with grass pollen respiratory allergy","volume":"42","author":"G Ricci","year":"2005","journal-title":"Mol Immunol"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1111\/j.1398-9995.2006.01258.x","article-title":"The impact of pollen-related food allergens on pollen allergy","volume":"63","author":"B Bohle","year":"2007","journal-title":"Allergy"},{"key":"ref40","unstructured":"Radauer C, Hoffmann-Sommergruber K (2004) Profilins. Plant food allergens. In: Mills ENC, Shewry PR, editors. Oxford: Blackwell Publishing. 105\u2013124."},{"key":"ref41","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1159\/000053797","article-title":"Multiple pollen sensitization: a molecular approach to the diagnosis","volume":"125","author":"A Mari","year":"2001","journal-title":"Int Arch Allergy Immunol"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1159\/000236171","article-title":"Profilin is a cross-reactive allergen in pollen and vegetable foods","volume":"98","author":"R van Ree","year":"1992","journal-title":"Int Arch Allergy Immunol"},{"key":"ref43","first-page":"140","article-title":"Allergic reaction after ingestion of orange blossom pollen","volume":"16","author":"D El-Qutob L\u00f3pez","year":"2006","journal-title":"J Investig Allergol Clin Immunol"},{"key":"ref44","first-page":"428","article-title":"Genomic identification of putative allergen genes in woodland strawberry (<italic>Fragaria vesca<\/italic>) and mandarin orange (<italic>Citrus clementina<\/italic>)","volume":"4","author":"KT Hyun","year":"2011","journal-title":"Plant Omics J"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1105\/tpc.110.074799","article-title":"Endoplasmic Reticulum\u2013 and Golgi-Localized Phospholipase A2 Plays Critical Roles in Arabidopsis Pollen Development and Germination","volume":"23","author":"HJ Kim","year":"2011","journal-title":"The Plant Cell"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0053680","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,9]],"date-time":"2020-05-09T15:40:42Z","timestamp":1589038842000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0053680"}},"subtitle":[],"editor":[{"given":"Phillip A.","family":"Stumbles","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2013,1,4]]},"references-count":45,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2013,1,4]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0053680","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,1,4]]}}}