{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T21:38:54Z","timestamp":1791149934591,"version":"4.1.0"},"reference-count":47,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2013,1,22]],"date-time":"2013-01-22T00:00:00Z","timestamp":1358812800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0054121","type":"journal-article","created":{"date-parts":[[2013,1,22]],"date-time":"2013-01-22T22:58:36Z","timestamp":1358895516000},"page":"e54121","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":20,"title":["Identification of Novel Protein-Protein Interactions of Yersinia pestis Type III Secretion System by Yeast Two Hybrid System"],"prefix":"10.1371","volume":"8","author":[{"given":"Huiying","family":"Yang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yafang","family":"Tan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingting","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liujun","family":"Tang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuehua","family":"Ke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaobiao","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoming","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruifu","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zongmin","family":"Du","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2013,1,22]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"1315","DOI":"10.1128\/MMBR.62.4.1315-1352.1998","article-title":"The virulence plasmid of Yersinia, an antihost genome","volume":"62","author":"GR Cornelis","year":"1998","journal-title":"Microbiol Mol Biol Rev"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1128\/CMR.10.1.35","article-title":"Yersinia pestis\u2013etiologic agent of plague","volume":"10","author":"RD Perry","year":"1997","journal-title":"Clin Microbiol Rev"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"1196","DOI":"10.1016\/S0140-6736(07)60566-2","article-title":"Plague","volume":"369","author":"MB Prentice","year":"2007","journal-title":"Lancet"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"964","DOI":"10.1002\/j.1460-2075.1994.tb06341.x","article-title":"Target cell contact triggers expression and polarized transfer of Yersinia YopE cytotoxin into mammalian cells","volume":"13","author":"R Rosqvist","year":"1994","journal-title":"Embo J"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"811","DOI":"10.1038\/nrmicro1526","article-title":"The type III secretion injectisome","volume":"4","author":"GR Cornelis","year":"2006","journal-title":"Nature Reviews Microbiology"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"1040","DOI":"10.1126\/science.1102610","article-title":"Structural insights into the assembly of the type III secretion needle complex","volume":"306","author":"TC Marlovits","year":"2004","journal-title":"Science"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1046\/j.1365-2958.1997.6141981.x","article-title":"The outer membrane component, YscC, of the Yop secretion machinery of Yersinia enterocolitica forms a ring-shaped multimeric complex","volume":"26","author":"M Koster","year":"1997","journal-title":"Mol Microbiol"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"4645","DOI":"10.1128\/JB.186.14.4645-4654.2004","article-title":"Structure and electrophysiological properties of the YscC secretin from the type III secretion system of Yersinia enterocolitica","volume":"186","author":"P Burghout","year":"2004","journal-title":"J Bacteriol"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"5366","DOI":"10.1128\/JB.186.16.5366-5375.2004","article-title":"Role of the pilot protein YscW in the biogenesis of the YscC secretin in Yersinia enterocolitica","volume":"186","author":"P Burghout","year":"2004","journal-title":"J Bacteriol"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1099\/mic.0.2007\/013045-0","article-title":"Membrane localization and topology of the Yersinia pestis YscJ lipoprotein","volume":"154","author":"E Silva-Herzog","year":"2008","journal-title":"Microbiology"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"468","DOI":"10.1038\/nsmb.1603","article-title":"A conserved structural motif mediates formation of the periplasmic rings in the type III secretion system","volume":"16","author":"T Spreter","year":"2009","journal-title":"Nat Struct Mol Biol"},{"key":"ref12","unstructured":"Cedric Cattin, Cornlis GR (2009) Stoichiometry Analysis of Type III Secretion Components by Fluorescent Labeling."},{"key":"ref13","doi-asserted-by":"crossref","first-page":"1561","DOI":"10.1128\/jb.176.6.1561-1569.1994","article-title":"YscN, the putative energizer of the Yersinia Yop secretion machinery","volume":"176","author":"S Woestyn","year":"1994","journal-title":"J Bacteriol"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1111\/j.1574-6968.2000.tb09086.x","article-title":"Interactions between type III secretion apparatus components from Yersinia pestis detected using the yeast two-hybrid system","volume":"186","author":"MW Jackson","year":"2000","journal-title":"FEMS Microbiology Letters"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"3525","DOI":"10.1128\/JB.188.10.3525-3534.2006","article-title":"Characterization of the Yersinia enterocolitica type III secretion ATPase YscN and its regulator, YscL","volume":"188","author":"B Blaylock","year":"2006","journal-title":"J Bacteriol"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"805","DOI":"10.1111\/j.1462-5822.2004.00432.x","article-title":"Conserved features of type III secretion","volume":"6","author":"AP Tampakaki","year":"2004","journal-title":"Cell Microbiol"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"1311","DOI":"10.1111\/j.1365-2958.2007.05871.x","article-title":"Function and molecular architecture of the Yersinia injectisome tip complex","volume":"65","author":"P Broz","year":"2007","journal-title":"Mol Microbiol"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1111\/j.1365-2958.2005.04534.x","article-title":"Characterization of a Type III secretion substrate specificity switch (T3S4 ) domain in YscP from Yersinia enterocolitica","volume":"56","author":"C Agrain","year":"2005","journal-title":"Molecular Microbiology"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"1415","DOI":"10.1111\/j.1365-2958.2005.04758.x","article-title":"Secretion of YscP from Yersinia enterocolitica is essential to control the length of the injectisome needle but not to change the type III secretion substrate specificity","volume":"57","author":"C Agrain","year":"2005","journal-title":"Mol Microbiol"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1046\/j.1365-2958.1999.01154.x","article-title":"Role of SycD, the chaperone of the Yersinia Yop translocators YopB and YopD","volume":"31","author":"C Neyt","year":"1999","journal-title":"Mol Microbiol"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"674","DOI":"10.1126\/science.1118476","article-title":"The V-antigen of Yersinia forms a distinct structure at the tip of injectisome needles","volume":"310","author":"CA Mueller","year":"2005","journal-title":"Science"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1083\/jcb.200205077","article-title":"<italic>Yersinia<\/italic> type III secretion: send in the effectors","volume":"158","author":"GR Cornelis","year":"2002","journal-title":"J Cell Biol"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1128\/MMBR.68.4.771-795.2004","article-title":"Process of protein transport by the type III secretion system","volume":"68","author":"P Ghosh","year":"2004","journal-title":"Microbiology and Molecular Biology Reviews"},{"key":"ref24","first-page":"735","article-title":"Assembly and function of type III secretory systems","volume":"54","author":"GR Cornelis","year":"2000","journal-title":"AnnuRevMicrobiol"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"807","DOI":"10.1046\/j.1365-2958.2003.03343.x","article-title":"Translocation of YopE and YopN into eukaryotic cells by Yersinia pestis yopN, tyeA, sycN, yscB and lcrG deletion mutants measured using a phosphorylatable peptide tag and phosphospecific antibodies","volume":"47","author":"JB Day","year":"2003","journal-title":"Mol Microbiol"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"1907","DOI":"10.1093\/emboj\/17.7.1907","article-title":"TyeA, a protein involved in control of Yop release and in translocation of Yersinia Yop effectors","volume":"17","author":"M Iriarte","year":"1998","journal-title":"Embo J"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"38693","DOI":"10.1074\/jbc.M405217200","article-title":"Novel protein-protein interactions of the <italic>Yersinia pestis<\/italic> type III secretion system elucidated with a matrix analysis by surface plasmon resonance and mass spectrometry","volume":"279","author":"W Swietnicki","year":"2004","journal-title":"J Biol Chem"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1038\/35097083","article-title":"Genome sequence of Yersinia pestis, the causative agent of plague","volume":"413","author":"J Parkhill","year":"2001","journal-title":"Nature"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"2763","DOI":"10.3390\/ijms10062763","article-title":"Yeast two-hybrid, a powerful tool for systems biology","volume":"10","author":"A Bruckner","year":"2009","journal-title":"Int J Mol Sci"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1016\/j.ijmm.2008.10.003","article-title":"Gene expression profiling of Yersinia pestis with deletion of lcrG, a known negative regulator for Yop secretion of type III secretion system","volume":"299","author":"Z Du","year":"2009","journal-title":"Int J Med Microbiol"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"1147","DOI":"10.1016\/j.jmb.2004.12.036","article-title":"Three-dimensional structure of a macromolecular assembly that regulates type III secretion in Yersinia pestis","volume":"346","author":"FD Schubot","year":"2005","journal-title":"J Mol Biol"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"829","DOI":"10.1128\/JB.186.3.829-841.2004","article-title":"Binding of SycH chaperone to YscM1 and YscM2 activates effector yop expression in Yersinia enterocolitica","volume":"186","author":"ED Cambronne","year":"2004","journal-title":"J Bacteriol"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"1261","DOI":"10.1111\/j.1365-2958.1996.tb02645.x","article-title":"The cytosolic SycE and SycH chaperones of Yersinia protect the region of YopE and YopH involved in translocation across eukaryotic cell membranes","volume":"20","author":"S Woestyn","year":"1996","journal-title":"Mol Microbiol"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1046\/j.1365-2958.2002.02752.x","article-title":"LcrQ and SycH function together at the Ysc type III secretion system in Yersinia pestis to impose a hierarchy of secretion","volume":"43","author":"CR Wulff-Strobel","year":"2002","journal-title":"Mol Microbiol"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1111\/j.1365-2958.1995.mmi_18020343.x","article-title":"Mutational analysis of the Yersinia enterocolitica virC operon: characterization of yscE, F, G, I, J, K required for Yop secretion and yscH encoding YopR","volume":"18","author":"A Allaoui","year":"1995","journal-title":"Mol Microbiol"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1046\/j.1365-2958.1998.01110.x","article-title":"A complex composed of SycN and YscB functions as a specific chaperone for YopN in Yersinia pestis","volume":"30","author":"JB Day","year":"1998","journal-title":"Mol Microbiol"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"3520","DOI":"10.1128\/jb.175.11.3520-3528.1993","article-title":"LcrG, a secreted protein involved in negative regulation of the low-calcium response in Yersinia pestis","volume":"175","author":"E Skryzpek","year":"1993","journal-title":"J Bacteriol"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"5082","DOI":"10.1128\/JB.183.17.5082-5091.2001","article-title":"LcrG-LcrV interaction is required for control of Yops secretion in Yersinia pestis","volume":"183","author":"JS Matson","year":"2001","journal-title":"J Bacteriol"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"7293","DOI":"10.1128\/jb.173.22.7293-7303.1991","article-title":"LcrD, a membrane-bound regulator of the Yersinia pestis low-calcium response","volume":"173","author":"GV Plano","year":"1991","journal-title":"J Bacteriol"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"3536","DOI":"10.1128\/jb.175.11.3536-3545.1993","article-title":"Multiple effects of lcrD mutations in Yersinia pestis","volume":"175","author":"GV Plano","year":"1993","journal-title":"J Bacteriol"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"4669","DOI":"10.1073\/pnas.071065798","article-title":"Polymerization of a single protein of the pathogen Yersinia enterocolitica into needles punctures eukaryotic cells","volume":"98","author":"E Hoiczyk","year":"2001","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1038\/nature04822","article-title":"Assembly of the inner rod determines needle length in the type III secretion injectisome","volume":"441","author":"TC Marlovits","year":"2006","journal-title":"Nature"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"4252","DOI":"10.1128\/JB.00328-08","article-title":"YscP and YscU Switch the Substrate Specificity of the Yersinia Type III Secretion System by Regulating Export of the Inner Rod Protein YscI","volume":"190","author":"SE Wood","year":"2008","journal-title":"Journal of Bacteriology"},{"key":"ref44","doi-asserted-by":"crossref","unstructured":"Yang F, Ke Y, Tan Y, Bi Y, Shi Q, et al.. (2010) Cell membrane is impaired, accompanied by enhanced type III secretion system expression in Yersinia pestis deficient in RovA regulator. PLoS One 5.","DOI":"10.1371\/journal.pone.0012840"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1016\/j.ab.2005.05.015","article-title":"Construction of a set Gateway-based destination vectors for high-throughput cloning and expression screening in Escherichia coli","volume":"343","author":"D Busso","year":"2005","journal-title":"Anal Biochem"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1006\/meth.2001.1190","article-title":"High-throughput yeast two-hybrid assays for large-scale protein interaction mapping","volume":"24","author":"AJ Walhout","year":"2001","journal-title":"Methods"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"2498","DOI":"10.1101\/gr.1239303","article-title":"Cytoscape: a software environment for integrated models of biomolecular interaction networks","volume":"13","author":"P Shannon","year":"2003","journal-title":"Genome Res"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0054121","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,7,21]],"date-time":"2020-07-21T00:24:57Z","timestamp":1595291097000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0054121"}},"subtitle":[],"editor":[{"given":"Fatah","family":"Kashanchi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2013,1,22]]},"references-count":47,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2013,1,22]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0054121","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,1,22]]}}}