{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T16:29:45Z","timestamp":1777566585129,"version":"3.51.4"},"reference-count":41,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2013,1,10]],"date-time":"2013-01-10T00:00:00Z","timestamp":1357776000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0054127","type":"journal-article","created":{"date-parts":[[2013,1,10]],"date-time":"2013-01-10T22:11:29Z","timestamp":1357855889000},"page":"e54127","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":43,"title":["A Homogeneous, High-Throughput Assay for Phosphatidylinositol 5-Phosphate 4-Kinase with a Novel, Rapid Substrate Preparation"],"prefix":"10.1371","volume":"8","author":[{"given":"Mindy I.","family":"Davis","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Atsuo T.","family":"Sasaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min","family":"Shen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brooke M.","family":"Emerling","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natasha","family":"Thorne","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sam","family":"Michael","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rajan","family":"Pragani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthew","family":"Boxer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazutaka","family":"Sumita","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Koh","family":"Takeuchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Douglas S.","family":"Auld","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhuyin","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lewis C.","family":"Cantley","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anton","family":"Simeonov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2013,1,10]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"1655","DOI":"10.1126\/science.296.5573.1655","article-title":"The phosphoinositide 3-kinase pathway","volume":"296","author":"LC Cantley","year":"2002","journal-title":"Science"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1038\/nrc2842","article-title":"Phosphoinositide signalling in cancer: beyond PI3K and PTEN","volume":"10","author":"TD Bunney","year":"2010","journal-title":"Nature Reviews Cancer"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"873","DOI":"10.1016\/j.ejcb.2006.04.007","article-title":"Regulation of chemotaxis by the orchestrated activation of Ras, PI3K, and TOR","volume":"85","author":"AT Sasaki","year":"2006","journal-title":"European Journal of Cell Biology"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1038\/nrm3290","article-title":"PI3K signalling: the path to discovery and understanding","volume":"13","author":"B Vanhaesebroeck","year":"2012","journal-title":"Nature Reviews Molecular Cell Biology"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1152\/physiol.00035.2008","article-title":"Mutations in Phosphoinositide Metabolizing Enzymes and Human Disease","volume":"24","author":"HJ McCrea","year":"2009","journal-title":"Physiology"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/S0968-0004(00)01543-7","article-title":"Phosphoinositide signaling and the regulation of membrane trafficking in yeast","volume":"25","author":"G Odorizzi","year":"2000","journal-title":"Trends in Biochemical Sciences"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1152\/physiol.00014.2009","article-title":"Phosphoinositide Signaling: New Tools and Insights","volume":"24","author":"T Balla","year":"2009","journal-title":"Physiology"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"1599","DOI":"10.1248\/bpb.30.1599","article-title":"The physiology of Phosphoinositides","volume":"30","author":"T Sasaki","year":"2007","journal-title":"Biological & Pharmaceutical Bulletin"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/S0009-3084(99)00019-5","article-title":"Pathways for phosphoinositide synthesis","volume":"98","author":"KF Tolias","year":"1999","journal-title":"Chemistry and Physics of Lipids"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1038\/36621","article-title":"A new pathway for synthesis of phosphatidylinositol-4,5-bisphosphate","volume":"390","author":"LE Rameh","year":"1997","journal-title":"Nature"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1042\/BST0350245","article-title":"Chemically targeting the PI3K family","volume":"35","author":"ZA Knight","year":"2007","journal-title":"Biochemical Society Transactions"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"2146","DOI":"10.1158\/0008-5472.CAN-09-4355","article-title":"Drugging the PI3 Kinome: From Chemical Tools to Drugs in the Clinic","volume":"70","author":"P Workman","year":"2010","journal-title":"Cancer Research"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1007\/s00424-011-1008-4","article-title":"Involvement of phosphatidylinositol 5-phosphate in insulin-stimulated glucose uptake in the L6 myotube model of skeletal muscle","volume":"462","author":"DL Grainger","year":"2011","journal-title":"Pflugers Archiv-European Journal of Physiology"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1016\/j.bbrc.2011.04.045","article-title":"Phosphatidylinositol 5-phosphate 4-kinase type II beta is required for vitamin D receptor-dependent E-cadherin expression in SW480 cells","volume":"408","author":"Z Kouchi","year":"2011","journal-title":"Biochemical and Biophysical Research Communications"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"5080","DOI":"10.1128\/MCB.24.11.5080-5087.2004","article-title":"Increased insulin sensitivity and reduced adiposity in phosphatidylinositol 5-phosphate 4-kinase beta\u2212\/\u2212 mice","volume":"24","author":"KA Lamia","year":"2004","journal-title":"Mol Cell Biol"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"3868","DOI":"10.1182\/blood-2005-07-2755","article-title":"Characterization of the megakaryocyte demarcation membrane system and its role in thrombopoiesis","volume":"107","author":"H Schulze","year":"2006","journal-title":"Blood"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"838","DOI":"10.1177\/1087057109339205","article-title":"High-throughput, cell-free, liposome-based approach for assessing in vitro activity of lipid kinases","volume":"14","author":"DJ Demian","year":"2009","journal-title":"J Biomol Screen"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"15141","DOI":"10.1021\/bi991571a","article-title":"The flattened face of type II beta phosphatidylinositol phosphate kinase binds acidic phospholipid membranes","volume":"38","author":"LM Burden","year":"1999","journal-title":"Biochemistry"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"829","DOI":"10.1016\/S0092-8674(00)81741-9","article-title":"Structure of type IIbeta phosphatidylinositol phosphate kinase: a protein kinase fold flattened for interfacial phosphorylation","volume":"94","author":"VD Rao","year":"1998","journal-title":"Cell"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/S0014-5793(00)01625-2","article-title":"Thrombin stimulation of platelets causes an increase in phosphatidylinositol 5-phosphate revealed by mass assay","volume":"475","author":"JB Morris","year":"2000","journal-title":"FEBS Lett"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1042\/BJ20100341","article-title":"PIP4Kbeta interacts with and modulates nuclear localization of the high-activity PtdIns5P-4-kinase isoform PIP4Kalpha","volume":"430","author":"Y Bultsma","year":"2010","journal-title":"Biochem J"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"5080","DOI":"10.1016\/S0021-9258(18)83702-4","article-title":"Characterization and purification of membrane-associated phosphatidylinositol-4-phosphate kinase from human red blood cells","volume":"264","author":"LE Ling","year":"1989","journal-title":"J Biol Chem"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/0076-6879(91)98010-4","article-title":"Identification and Quantification of Polyphosphoinositides Produced in Response to Platelet-Derived Growth-Factor Stimulation","volume":"198","author":"LA Serunian","year":"1991","journal-title":"Methods in Enzymology"},{"key":"ref24","first-page":"79","article-title":"Compound Management for Quantitative High-Throughput Screening","volume":"13","author":"A Yasgar","year":"2008","journal-title":"JALA"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"11473","DOI":"10.1073\/pnas.0604348103","article-title":"Quantitative high-throughput screening: a titration-based approach that efficiently identifies biological activities in large chemical libraries","volume":"103","author":"J Inglese","year":"2006","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"2372","DOI":"10.1021\/jm701302v","article-title":"Characterization of chemical libraries for luciferase inhibitory activity","volume":"51","author":"DS Auld","year":"2008","journal-title":"J Med Chem"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1177\/108705702237676","article-title":"A versatile high-throughput screen for inhibitors of lipid kinase activity: development of an immobilized phospholipid plate assay for phosphoinositide 3-kinase gamma","volume":"7","author":"K Fuchikami","year":"2002","journal-title":"J Biomol Screen"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"906","DOI":"10.1177\/1087057108324498","article-title":"A high-throughput liposome substrate assay with automated lipid extraction process for PI 3-kinase","volume":"13","author":"T Lingaraj","year":"2008","journal-title":"J Biomol Screen"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"2385","DOI":"10.1021\/jm061317y","article-title":"A high-throughput screen for aggregation-based inhibition in a large compound library","volume":"50","author":"BY Feng","year":"2007","journal-title":"Journal of Medicinal Chemistry"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1016\/j.chembiol.2005.04.011","article-title":"Features of selective kinase inhibitors","volume":"12","author":"ZA Knight","year":"2005","journal-title":"Chem Biol"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"606","DOI":"10.1089\/adt.2009.0230","article-title":"Comparison of bioluminescent kinase assays using substrate depletion and product formation","volume":"7","author":"C Tanega","year":"2009","journal-title":"Assay Drug Dev Technol"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1177\/1087057105285611","article-title":"The minimum significant ratio: A statistical parameter to characterize the reproducibility of potency estimates from concentration-response assays and estimation by replicate-experiment studies","volume":"11","author":"BJ Eastwood","year":"2006","journal-title":"Journal of Biomolecular Screening"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1089\/adt.2009.0223","article-title":"Evaluating the Utility of a Bioluminescent ADP-Detecting Assay for Lipid Kinases","volume":"7","author":"J Vidugiriene","year":"2009","journal-title":"Assay and Drug Development Technologies"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"560","DOI":"10.1089\/adt.2009.0222","article-title":"ADP-Glo: A Bioluminescent and Homogeneous ADP Monitoring Assay for Kinases","volume":"7","author":"H Zegzouti","year":"2009","journal-title":"Assay and Drug Development Technologies"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"1450","DOI":"10.1021\/jm8014525","article-title":"A basis for reduced chemical library inhibition of firefly luciferase obtained from directed evolution","volume":"52","author":"DS Auld","year":"2009","journal-title":"Journal of Medicinal Chemistry"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"1060","DOI":"10.1016\/j.chembiol.2012.07.015","article-title":"Firefly luciferase in chemical biology: A compendium of inhibitors, mechanistic evaluation of chemotypes, and suggested use as a reporter","volume":"19","author":"N Thorne","year":"2012","journal-title":"Chemistry and Biology"},{"key":"ref37","unstructured":"Strelow J, Dewe W, Iversen P, Brooks H, Radding J (2004) Mechanism of Action assays for Enzymes. In: Sittampalam GS, Gal-Edd N, Arkin M, Auld D, Austin C <etal>et al<\/etal>., editors. Assay Guidance Manual. Bethesda, MD: Eli Lilly &amp; Company and the National Center for Advancing Translational Sciences. Available: <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/www.ncbi.nlm.nih.gov\/books\/NBK92001\/\" xlink:type=\"simple\">http:\/\/www.ncbi.nlm.nih.gov\/books\/NBK92001\/<\/ext-link>. Accessed 2012 Nov 1."},{"key":"ref38","doi-asserted-by":"crossref","first-page":"15705","DOI":"10.1021\/bi001516y","article-title":"Substrate competitive inhibitors of IGF-1 receptor kinase","volume":"39","author":"G Blum","year":"2000","journal-title":"Biochemistry"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"6260","DOI":"10.1021\/bi962553l","article-title":"Phosphorylation site-specific inhibition of platelet-derived growth factor beta-receptor autophosphorylation by the receptor blocking tyrphostin AG1296","volume":"36","author":"M Kovalenko","year":"1997","journal-title":"Biochemistry"},{"key":"ref40","first-page":"673","article-title":"Kinetics of inhibition by tyrphostins of the tyrosine kinase activity of the epidermal growth factor receptor and analysis by a new computer program","volume":"45","author":"I Posner","year":"1994","journal-title":"Mol Pharmacol"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1089\/adt.2008.150","article-title":"A Robotic Platform for Quantitative High-Throughput Screening","volume":"6","author":"S Michael","year":"2008","journal-title":"Assay and Drug Development Technologies"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0054127","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,5]],"date-time":"2022-02-05T18:49:24Z","timestamp":1644086964000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0054127"}},"subtitle":[],"editor":[{"given":"Paul","family":"Taylor","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2013,1,10]]},"references-count":41,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2013,1,10]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0054127","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,1,10]]}}}