{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T07:10:24Z","timestamp":1792653024672,"version":"4.5.0"},"reference-count":66,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2013,2,12]],"date-time":"2013-02-12T00:00:00Z","timestamp":1360627200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0056759","type":"journal-article","created":{"date-parts":[[2013,2,12]],"date-time":"2013-02-12T22:12:19Z","timestamp":1360707139000},"page":"e56759","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":143,"title":["RBC-NOS-Dependent S-Nitrosylation of Cytoskeletal Proteins Improves RBC Deformability"],"prefix":"10.1371","volume":"8","author":[{"given":"Marijke","family":"Grau","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebastian","family":"Pauly","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jamal","family":"Ali","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katja","family":"Walpurgis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario","family":"Thevis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wilhelm","family":"Bloch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank","family":"Suhr","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2013,2,12]]},"reference":[{"key":"ref1","first-page":"63","article-title":"Heme-dependent activation of soluble guanylate cyclase by nitric oxide: regulation of enzyme activity by porphyrins and metalloporphyrins","volume":"26","author":"LJ Ignarro","year":"1989","journal-title":"Semin Hematol"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1111\/j.1476-5381.1990.tb12709.x","article-title":"Characterization of the L-arginine:nitric oxide pathway in human platelets","volume":"101","author":"MW Radomski","year":"1990","journal-title":"Br J Pharmacol"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1038\/nchembio734","article-title":"Nitrite is a signaling molecule and regulator of gene expression in mammalian tissues","volume":"1","author":"NS Bryan","year":"2005","journal-title":"Nat Chem Biol"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1038\/nchembio1005-245","article-title":"Nitrite as an intrinsic signaling molecule","volume":"1","author":"MT Gladwin","year":"2005","journal-title":"Nat Chem Biol"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"1498","DOI":"10.1038\/nm954","article-title":"Nitrite reduction to nitric oxide by deoxyhemoglobin vasodilates the human circulation","volume":"9","author":"K Cosby","year":"2003","journal-title":"Nat Med"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"566","DOI":"10.1182\/blood-2005-07-2668","article-title":"Hypoxia, red blood cells, and nitrite regulate NO-dependent hypoxic vasodilation","volume":"107","author":"JH Crawford","year":"2006","journal-title":"Blood"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"915","DOI":"10.1161\/01.ATV.0000161048.72004.c2","article-title":"NO generation from nitrite and its role in vascular control","volume":"25","author":"JO Lundberg","year":"2005","journal-title":"Arterioscler Thromb Vasc Biol"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"46349","DOI":"10.1074\/jbc.M307572200","article-title":"Active nitric oxide produced in the red cell under hypoxic conditions by deoxyhemoglobin-mediated nitrite reduction","volume":"278","author":"E Nagababu","year":"2003","journal-title":"J Biol Chem"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1161\/CIRCULATIONAHA.111.087155","article-title":"Nitrite regulates hypoxic vasodilation via myoglobin-dependent nitric oxide generation","volume":"126","author":"M Totzeck","year":"2012","journal-title":"Circulation"},{"key":"ref10","doi-asserted-by":"crossref","unstructured":"Ulker P, Gunduz F, Meiselman HJ, Baskurt OK (2012) Nitric oxide generated by red blood cells following exposure to shear stress dilates isolated small mesenteric arteries under hypoxic conditions. Clin Hemorheol Microcirc","DOI":"10.3233\/CH-2012-1618"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1016\/S1471-4914(03)00028-5","article-title":"S-nitrosylation in health and disease","volume":"9","author":"MW Foster","year":"2003","journal-title":"Trends Mol Med"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.mam.2004.09.002","article-title":"The basics about nitric oxide","volume":"26","author":"R Bruckdorfer","year":"2005","journal-title":"Mol Aspects Med"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.cardiores.2004.01.013","article-title":"S-nitrosylation: a potential new paradigm in signal transduction","volume":"62","author":"A Martinez-Ruiz","year":"2004","journal-title":"Cardiovasc Res"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"re1","DOI":"10.1126\/stke.2001.86.re1","article-title":"S-nitrosylation is emerging as a specific and fundamental posttranslational protein modification: head-to-head comparison with O-phosphorylation","volume":"2001","author":"P Lane","year":"2001","journal-title":"Sci STKE"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"1036","DOI":"10.1007\/s000180050354","article-title":"S-nitrosylation of proteins","volume":"55","author":"MC Broillet","year":"1999","journal-title":"Cell Mol Life Sci"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1038\/nrm1569","article-title":"Protein S-nitrosylation: purview and parameters","volume":"6","author":"DT Hess","year":"2005","journal-title":"Nat Rev Mol Cell Biol"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"1328","DOI":"10.1126\/science.1093891","article-title":"S-nitrosylation of parkin regulates ubiquitination and compromises parkin&apos;s protective function","volume":"304","author":"KK Chung","year":"2004","journal-title":"Science"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"2673","DOI":"10.1161\/hc4601.099485","article-title":"Endothelial dysfunction, oxidative stress, and risk of cardiovascular events in patients with coronary artery disease","volume":"104","author":"T Heitzer","year":"2001","journal-title":"Circulation"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1016\/j.freeradbiomed.2005.08.025","article-title":"Plasma nitrite concentrations reflect the degree of endothelial dysfunction in humans","volume":"40","author":"P Kleinbongard","year":"2006","journal-title":"Free Radic Biol Med"},{"key":"ref20","first-page":"5","article-title":"A study of humoral factors in carcinoma cervix","volume":"35","author":"J Agarwal","year":"1992","journal-title":"Indian J Pathol Microbiol"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"1214","DOI":"10.1016\/S0895-7061(96)00257-9","article-title":"Erythrocytes may synthesize their own nitric oxide","volume":"9","author":"BC Jubelin","year":"1996","journal-title":"Am J Hypertens"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"2943","DOI":"10.1182\/blood-2005-10-3992","article-title":"Red blood cells express a functional endothelial nitric oxide synthase","volume":"107","author":"P Kleinbongard","year":"2006","journal-title":"Blood"},{"key":"ref23","doi-asserted-by":"crossref","unstructured":"Cortese-Krott MM, Rodriguez-Mateos A, Sansone R, Kuhnle GG, Thasian-Sivarajah S, <etal>et al<\/etal>.. (2012) Human red blood cells at work: identification and visualization of erythrocytic eNOS activity in health and disease. Blood","DOI":"10.1182\/blood-2012-07-442277"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"e45982","DOI":"10.1371\/journal.pone.0045982","article-title":"Moderate Exercise Promotes Human RBC-NOS Activity, NO Production and Deformability through Akt Kinase Pathway","volume":"7","author":"F Suhr","year":"2012","journal-title":"PLoS One"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"C378","DOI":"10.1152\/ajpcell.00543.2008","article-title":"Erythropoietin activates nitric oxide synthase in murine erythrocytes","volume":"297","author":"D Mihov","year":"2009","journal-title":"Am J Physiol Cell Physiol"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/j.niox.2008.10.004","article-title":"Intensive exercise induces changes of endothelial nitric oxide synthase pattern in human erythrocytes","volume":"20","author":"F Suhr","year":"2009","journal-title":"Nitric Oxide"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"H1577","DOI":"10.1152\/ajpheart.00665.2002","article-title":"Effects of nitric oxide on red blood cell deformability","volume":"284","author":"M Bor-Kucukatay","year":"2003","journal-title":"Am J Physiol Heart Circ Physiol"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1016\/j.niox.2011.03.003","article-title":"Shear stress activation of nitric oxide synthase and increased nitric oxide levels in human red blood cells","volume":"24","author":"P Ulker","year":"2011","journal-title":"Nitric Oxide"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"746","DOI":"10.1111\/j.1476-5381.1990.tb14151.x","article-title":"Characterization of three inhibitors of endothelial nitric oxide synthase in vitro and in vivo","volume":"101","author":"DD Rees","year":"1990","journal-title":"Br J Pharmacol"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1111\/j.1476-5381.1991.tb12159.x","article-title":"Identification of N-iminoethyl-L-ornithine as an irreversible inhibitor of nitric oxide synthase in phagocytic cells","volume":"102","author":"TB McCall","year":"1991","journal-title":"Br J Pharmacol"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"1719","DOI":"10.1242\/jeb.204.10.1719","article-title":"Nitric oxide modulates cardiac performance in the heart of Anguilla anguilla","volume":"204","author":"S Imbrogno","year":"2001","journal-title":"J Exp Biol"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1016\/S0960-9822(99)80371-6","article-title":"The Akt kinase signals directly to endothelial nitric oxide synthase","volume":"9","author":"BJ Michell","year":"1999","journal-title":"Curr Biol"},{"key":"ref33","first-page":"2036","article-title":"Nitric oxide mediates angiogenesis in vivo and endothelial cell growth and migration in vitro promoted by substance P. J Clin Invest","volume":"94","author":"M Ziche","year":"1994"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"1198","DOI":"10.1006\/bbrc.1993.1543","article-title":"Nitric oxide promotes DNA synthesis and cyclic GMP formation in endothelial cells from postcapillary venules","volume":"192","author":"M Ziche","year":"1993","journal-title":"Biochem Biophys Res Commun"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1042\/bj3310713","article-title":"Control of growth and differentiation of normal human epithelial cells through the manipulation of reactive nitrogen species","volume":"3)","author":"G Vallette","year":"1998","journal-title":"Biochem J 331 (Pt"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"644","DOI":"10.1016\/j.lfs.2006.10.010","article-title":"2-(4-Carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide potentiates nitrosation of a heterocyclic amine carcinogen by nitric oxide","volume":"80","author":"VM Lakshmi","year":"2007","journal-title":"Life Sci"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"1121","DOI":"10.1161\/01.HYP.23.6.1121","article-title":"Nitric oxide synthase isozymes. Characterization, purification, molecular cloning, and functions","volume":"23","author":"U Forstermann","year":"1994","journal-title":"Hypertension"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1046\/j.1365-2125.1998.00803.x","article-title":"L-arginine-induced vasodilation in healthy humans: pharmacokinetic-pharmacodynamic relationship","volume":"46","author":"SM Bode-Boger","year":"1998","journal-title":"Br J Clin Pharmacol"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1097\/00004872-199306000-00008","article-title":"Role of L-arginine-nitric oxide pathway in hypertension","volume":"11","author":"K Hishikawa","year":"1993","journal-title":"J Hypertens"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"572","DOI":"10.1016\/0140-6736(92)90865-Z","article-title":"Accumulation of an endogenous inhibitor of nitric oxide synthesis in chronic renal failure","volume":"339","author":"P Vallance","year":"1992","journal-title":"Lancet"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"1842","DOI":"10.1161\/01.CIR.98.18.1842","article-title":"Asymmetric dimethylarginine (ADMA): a novel risk factor for endothelial dysfunction: its role in hypercholesterolemia","volume":"98","author":"RH Boger","year":"1998","journal-title":"Circulation"},{"key":"ref42","first-page":"1069","article-title":"Short-term effect of the HMG-CoA reductase inhibitor rosuvastatin on erythrocyte nitric oxide synthase activity","volume":"3","author":"B Ludolph","year":"2007","journal-title":"Vasc Health Risk Manag"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"790","DOI":"10.1016\/S0891-5849(03)00406-4","article-title":"Plasma nitrite reflects constitutive nitric oxide synthase activity in mammals","volume":"35","author":"P Kleinbongard","year":"2003","journal-title":"Free Radic Biol Med"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"12814","DOI":"10.1073\/pnas.221381098","article-title":"Plasma nitrite rather than nitrate reflects regional endothelial nitric oxide synthase activity but lacks intrinsic vasodilator action","volume":"98","author":"T Lauer","year":"2001","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"765","DOI":"10.1016\/S0008-6363(98)00259-4","article-title":"Serum nitrite sensitively reflects endothelial NO formation in human forearm vasculature: evidence for biochemical assessment of the endothelial L-arginine-NO pathway","volume":"41","author":"M Kelm","year":"1999","journal-title":"Cardiovasc Res"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1016\/j.freeradbiomed.2006.05.001","article-title":"The measurement of blood and plasma nitrite by chemiluminescence: pitfalls and solutions","volume":"41","author":"MM Pelletier","year":"2006","journal-title":"Free Radic Biol Med"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1016\/S0076-6879(08)01216-0","article-title":"Reductive gas-phase chemiluminescence and flow injection analysis for measurement of the nitric oxide pool in biological matrices","volume":"441","author":"U Hendgen-Cotta","year":"2008","journal-title":"Methods Enzymol"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.jchromb.2007.02.002","article-title":"Recent methodological advances in the analysis of nitrite in the human circulation: nitrite as a biochemical parameter of the L-arginine\/NO pathway","volume":"851","author":"M Grau","year":"2007","journal-title":"J Chromatogr B Analyt Technol Biomed Life Sci"},{"key":"ref49","first-page":"675","article-title":"Nitrosylation. the prototypic redox-based signaling mechanism","volume":"106","author":"JS Stamler","year":"2001","journal-title":"Cell"},{"key":"ref50","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1038\/35055104","article-title":"Protein S-nitrosylation: a physiological signal for neuronal nitric oxide","volume":"3","author":"SR Jaffrey","year":"2001","journal-title":"Nat Cell Biol"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"3263","DOI":"10.1002\/pmic.201200280","article-title":"Storage-induced changes of the cytosolic red blood cell proteome analyzed by 2D DIGE and high-resolution\/high-accuracy MS","volume":"12","author":"K Walpurgis","year":"2012","journal-title":"Proteomics"},{"key":"ref52","first-page":"1","article-title":"The Laser-assisted Optical Rotational Cell Analyzer (LORCA) as red blood cell aggregometer","volume":"25","author":"MR Hardeman","year":"2001","journal-title":"Clin Hemorheol Microcirc"},{"key":"ref53","doi-asserted-by":"crossref","first-page":"75","DOI":"10.3233\/CH-2009-1202","article-title":"New guidelines for hemorheological laboratory techniques","volume":"42","author":"OK Baskurt","year":"2009","journal-title":"Clin Hemorheol Microcirc"},{"key":"ref54","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0014-5793(00)01686-0","article-title":"Endogenous nitric oxide synthase inhibitors are responsible for the L-arginine paradox","volume":"478","author":"D Tsikas","year":"2000","journal-title":"FEBS Lett"},{"key":"ref55","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1016\/j.molmed.2009.06.007","article-title":"Protein S-nitrosylation in health and disease: a current perspective","volume":"15","author":"MW Foster","year":"2009","journal-title":"Trends Mol Med"},{"key":"ref56","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1186\/cc2353","article-title":"Bench-to-bedside review: microvascular dysfunction in sepsis--hemodynamics, oxygen transport, and nitric oxide","volume":"7","author":"RM Bateman","year":"2003","journal-title":"Crit Care"},{"key":"ref57","first-page":"143","article-title":"Rheology of the microcirculation","volume":"29","author":"AR Pries","year":"2003","journal-title":"Clin Hemorheol Microcirc"},{"key":"ref58","doi-asserted-by":"crossref","first-page":"2427","DOI":"10.1242\/jcs.01165","article-title":"eNOS at a glance","volume":"117","author":"WC Sessa","year":"2004","journal-title":"J Cell Sci"},{"key":"ref59","doi-asserted-by":"crossref","first-page":"444","DOI":"10.1067\/mlc.2000.106805","article-title":"Normal circulating adult human red blood cells contain inactive NOS proteins","volume":"135","author":"ES Kang","year":"2000","journal-title":"J Lab Clin Med"},{"key":"ref60","doi-asserted-by":"crossref","first-page":"1322","DOI":"10.1182\/blood-2011-11-393124","article-title":"Doubts concerning functional endothelial nitric oxide synthase in human erythrocytes","volume":"119","author":"A Bohmer","year":"2012","journal-title":"Blood"},{"key":"ref61","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1007\/s10540-006-9024-x","article-title":"Spectrin organization and dynamics: new insights","volume":"26","author":"A Chakrabarti","year":"2006","journal-title":"Biosci Rep"},{"key":"ref62","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1016\/j.mvr.2012.02.004","article-title":"The effects of membrane cholesterol and simvastatin on red blood cell deformability and ATP release","volume":"83","author":"AM Forsyth","year":"2012","journal-title":"Microvasc Res"},{"key":"ref63","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.tracli.2010.05.008","article-title":"Erythrocyte adducin: a structural regulator of the red blood cell membrane","volume":"17","author":"T Franco","year":"2010","journal-title":"Transfus Clin Biol"},{"key":"ref64","first-page":"21","article-title":"The diagnostic relevance of red cell rigidity","volume":"19","author":"R Banerjee","year":"1998","journal-title":"Clin Hemorheol Microcirc"},{"key":"ref65","doi-asserted-by":"crossref","first-page":"2081","DOI":"10.1016\/j.jacc.2006.08.069","article-title":"Total cholesterol content of erythrocyte membranes is increased in patients with acute coronary syndrome: a new marker of clinical instability?","volume":"49","author":"DN Tziakas","year":"2007","journal-title":"J Am Coll Cardiol"},{"key":"ref66","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1016\/j.freeradbiomed.2003.11.032","article-title":"The biochemistry of nitric oxide, nitrite, and hemoglobin: role in blood flow regulation","volume":"36","author":"MT Gladwin","year":"2004","journal-title":"Free Radic Biol Med"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0056759","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,8]],"date-time":"2022-02-08T14:18:55Z","timestamp":1644329935000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0056759"}},"subtitle":[],"editor":[{"given":"Carlo","family":"Gaetano","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2013,2,12]]},"references-count":66,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2013,2,12]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0056759","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,2,12]]}}}