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Antimicrob. Agents Chemother","volume":"48","author":"R Bonnet","year":"2004"},{"key":"ref2","first-page":"269","article-title":"A standard numbering scheme for the class A \u03b2-lactamases. Biochem. J","volume":"276","author":"RP Ambler","year":"1991"},{"key":"ref3","first-page":"1211","article-title":"A functional classification scheme for beta-lactamases and its correlation with molecular structure. Antimicrob. Agents Chemother","volume":"39","author":"K Bush","year":"1995"},{"key":"ref4","unstructured":"Coque TM, Baquero F, Canton R (2008) Increasing prevalence of ESBL-producing <italic>Enterobacteriaceae<\/italic> in Europe. Euro Surveill. 13(47): pii_19044. <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/www.eurosurveillance.org\/ViewArticle.aspx?ArticleId_19044\" xlink:type=\"simple\">http:\/\/www.eurosurveillance.org\/ViewArticle.aspx?ArticleId_19044<\/ext-link>."},{"key":"ref5","first-page":"i3","article-title":"The changing epidemiology of resistance. J. Antimicrob. Chemother","volume":"6","author":"PM Hawkey","year":"2009"},{"key":"ref6","first-page":"223","article-title":"Identification of CTX-M type extended-spectrum-beta-lactamase genes using real-time PCR and pyrosequencing. Antimicrob. Agents Chemother","volume":"51","author":"T Naas","year":"2007"},{"key":"ref7","first-page":"4361","article-title":"Distant and new mutations in CTX-M-1 \u03b2-lactamase affect cefotaxime hydrolysis. Antimicrob. Agents Chemother","volume":"55","author":"FJ P\u00e9rez-Llarena","year":"2011"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1007\/BF01704610","article-title":"A new plasmidic cefotaximase from patients infected with <italic>Salmonella typhimurium<\/italic>","volume":"20","author":"A Bauernfeind","year":"1992","journal-title":"Infection"},{"key":"ref9","first-page":"237","article-title":"Plasmid mediated extended-spectrum \u03b2-lactamase (CTX-M-3 like) from India and gene association with insertion sequence ISEcp1. FEMS Microbiol. Lett","volume":"201","author":"A Karim","year":"2001"},{"key":"ref10","first-page":"2269","article-title":"Cloning and sequence of the gene encoding a cefotaxime-hydrolyzing class A \u03b2-lactamase isolated from <italic>Escherichia coli. Antimicrob.<\/italic> Agents Chemother","volume":"39","author":"Y Ishii","year":"1995"},{"key":"ref11","first-page":"827","article-title":"Cefotaxime-resistant <italic>Enterobacteriaceae<\/italic> isolates from a hospital in Warsaw, Poland: identification of a new CTX-M-3 cefotaxime-hydrolyzing \u03b2-lactamase that is closely related to the CTX-M-1\/MEN-1 enzyme. Antimicrob. Agents Chemother","volume":"42","author":"M Gniadkowski","year":"1998"},{"key":"ref12","first-page":"657","article-title":"Extended-spectrum \u03b2- lactamases: a clinical update. Clin. Microbiol.Rev","volume":"18","author":"DL Paterson","year":"2005"},{"key":"ref13","first-page":"2","article-title":"Infected foot ulcers in male and female diabetic patients: a clinico-bioinformative study. Ann. Clin. Microbiol. Antimicrob","volume":"9","author":"S Shakil","year":"2010"},{"key":"ref14","first-page":"558","article-title":"Alarming \u03b2-lactamase-mediated resistance in multi-drug resistant <italic>Enterobacteriaceae<\/italic>. Curr. Opin. Microbiol","volume":"13","author":"K Bush","year":"2010"},{"key":"ref15","first-page":"224","article-title":"Bench-to-bedside review: the role of \u03b2-lactamases in antibiotic-resistant Gram-negative infections. Crit","volume":"14","author":"K Bush","year":"2010","journal-title":"Care"},{"key":"ref16","first-page":"3465","article-title":"Exploring the inhibition of CTX-M-9 by \u03b2-lactamase inhibitors and carbapenems. Antimicrob. Agents Chemother","volume":"55","author":"CR Bethal","year":"2011"},{"key":"ref17","first-page":"9422","article-title":"Molecular dynamics at the root of expansion of function in the M69L inhibitor-resistant TEM \u03b2-lactamase from <italic>Escherichia coli<\/italic>. J. Am. Chem. Soc","volume":"124","author":"SO Meroueh","year":"2002"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"14111","DOI":"10.1021\/bi0487903","article-title":"Inhibitor-resistant class A \u03b2-lactamase: Consequences of the Ser130-to-Gly mutation seen in apo and Tazobactam structures of the SHV-1 variant","volume":"43","author":"T Sun","year":"2004","journal-title":"Biochemistry"},{"key":"ref19","first-page":"839","article-title":"Beta-lactamases of increasing clinical importance. Curr. Pharm. Des","volume":"5(11)","author":"K Bush","year":"1999"},{"key":"ref20","first-page":"881","article-title":"Regulation of inducible AmpC beta-lactamase expression among <italic>Enterobacteriaceae<\/italic>. Curr. Pharm. Des","volume":"5(11)","author":"ND Hanson","year":"1999"},{"key":"ref21","first-page":"2330","article-title":"The complex structure and antimicrobial activity of a non-beta-lactam inhibitor of AmpC beta-lactamase. Protein Sci","volume":"8(11)","author":"RA Powers","year":"1999"},{"key":"ref22","first-page":"1012","article-title":"Structure-based discovery of a novel, noncovalent inhibitor of AmpC \u03b2-lactamase","volume":"10","author":"RA Power","year":"2002","journal-title":"Structure"},{"key":"ref23","first-page":"948","article-title":"Acquisition of extended-spectrum \u03b2-lactamase producing <italic>Escherichia coli<\/italic> strains in male and female infants admitted to a neonatal intensive care unit: molecular epidemiology and analysis of risk factors","volume":"59","author":"S Shakil","year":"2010","journal-title":"Journal of Molecular Medicine"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"e40319","DOI":"10.1371\/journal.pone.0040319","article-title":"Efficacy of E. officinalis on the Cariogenic Properties of Streptococcus mutans: A Novel and Alternative Approach to Suppress Quorum-Sensing Mechanism","volume":"7(7)","author":"S Hasan","year":"2012","journal-title":"PLoS ONE"},{"key":"ref25","unstructured":"Clinical and Laboratory Standards Institute (2011) Performance standards for antimicrobial susceptibility testing: 21<sup>st<\/sup> informational supplement. M100-S21. CLSI, Wayne, PA."},{"key":"ref26","first-page":"1861","article-title":"CTX-M-93, a variant lacking penicillin hydrolytic activity. Antimicrob. Agents Chemother","volume":"55(5)","author":"L Djamdjin","year":"2011"},{"key":"ref27","first-page":"5132","article-title":"Mechanistic studies of the inactivation of TEM-1 and P99 by NXL104, a novel non-\u03b2-lactam \u03b2-lactamase inhibitor. Antimicrob. Agents Chemother","volume":"54(12)","author":"T Stachyra","year":"2010"},{"key":"ref28","first-page":"1608","article-title":"Use of chromosomal class A \u03b2-lactamase of <italic>Mycobacterium fortuitum<\/italic> D316 to study potentially poor substrates and inhibitory \u03b2-lactam compounds. Antimicrob. Agents Chemother","volume":"38","author":"M Galleni","year":"1994"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"e17159","DOI":"10.1371\/journal.pone.0017159","article-title":"Functional role of Glutamine 28 and Arginine 39 in double stranded RNA cleavage by Human Pancreatic Ribonuclease","volume":"6(3)","author":"MT Rehman","year":"2011","journal-title":"PLoS ONE"},{"key":"ref30","first-page":"2215","article-title":"Kinetic study of two novel enantiomeric tricyclic \u03b2-lactams which efficiently inactivate class C \u03b2-lactamases. Antimicrob. Agents Chemother","volume":"45","author":"M Vilar","year":"2001"},{"key":"ref31","first-page":"705","article-title":"Interaction between active-site serine \u03b2-lactamases and mechanism-based inactivators: a kinetic study and an overview. Biochem. J","volume":"295","author":"A Matagne","year":"1993"},{"key":"ref32","doi-asserted-by":"crossref","unstructured":"Waley SG (1992) \u03b2-lactamase: mechanism of action, p.198\u2013228. <italic>In<\/italic> M I Page (ed.), The chemistry of \u03b2-lactams. Blackie A and P, London, United Kingdom.","DOI":"10.1007\/978-94-011-2928-2_6"},{"key":"ref33","first-page":"187","article-title":"CHARMM: A program for macromolecular energy, minimization, and dynamics calculations. J. Comp. Chem","volume":"4","author":"BR Brooks","year":"1983"},{"key":"ref34","first-page":"177","article-title":"ZINC\u2013a free database of commercially available compounds for virtual screening. J. Chem. Inf. Model","volume":"45","author":"JJ Irwin","year":"2005"},{"key":"ref35","first-page":"43","article-title":"Molecular recognition of receptor sites using a genetic algorithim with a description of desolvation. J. Mol. Biol","volume":"245","author":"G Jones","year":"1995"},{"key":"ref36","first-page":"1936","article-title":"A novel CTX-M \u03b2-lactamase (CTX-M-8) in cefotaxime-resistant <italic>Enterobacteriacae<\/italic> isolated in Brazil. Antimicrob. Agents Chemother","volume":"44","author":"R Bonnet","year":"2000"},{"key":"ref37","first-page":"178","article-title":"beta-Lactamases: Which ones are clinically important? Drug Resist. Updates","volume":"3(3)","author":"LB Rice","year":"2000"},{"key":"ref38","first-page":"1433","article-title":"The impact of antimicrobial resistance on health and economic outcomes. Clin. Infect. Dis","volume":"36","author":"S Cosgrove","year":"2003"},{"key":"ref39","doi-asserted-by":"crossref","unstructured":"Chambers HF (1999) Penicillin-binding protein-mediated resistance in <italic>Pneumococci<\/italic> and <italic>Staphylococci<\/italic>. J. Infect. Dis. (Suppl. 2): 353\u2013359.","DOI":"10.1086\/513854"},{"key":"ref40","first-page":"159","article-title":"Efflux-mediated drug resistance in bacteria","volume":"64(2)","author":"XZ Li","year":"2004","journal-title":"Drugs"},{"key":"ref41","first-page":"2415","article-title":"Efflux pump-mediated intrinsic drug resistance in <italic>Mycobacterium smegmatis<\/italic>. Antimicrob. Agents Chemother","volume":"48(7)","author":"XZ Li","year":"2004"},{"key":"ref42","first-page":"851","article-title":"Resistance to Imipenem, Cefepime, and Cefpirome associated with mutation in Omp36 osmoporin of <italic>Enterobacter<\/italic> a<italic>erogenes<\/italic>. Biochem. Biophys. Res. Commun","volume":"317(3)","author":"A Thiolas","year":"2004"},{"key":"ref43","first-page":"4632","article-title":"Structure-based optimization of a non-\u03b2-lactam lead results in inhibitors that do not up-regulate \u03b2-lactamase expression in cell culture. J. Am. Chem. Soc","volume":"127","author":"D Tondi","year":"2005"},{"key":"ref44","first-page":"153","article-title":"Molecular basis of beta-lactamase induction in bacteria. Antimicrob. Agents Chemother","volume":"37(2)","author":"PM Bennett","year":"1993"},{"key":"ref45","first-page":"1391","article-title":"The development of beta-lactam antibiotics in response to the evolution of beta-lactamases. Pharm. Res","volume":"18(10)","author":"SY Essack","year":"2001"},{"key":"ref46","first-page":"1031","article-title":"Biochemical analysis of the ceftazidime-hydrolysing extended-spectrum \u03b2-lactamase CTX-M-15 and of its structurally related \u03b2-lactamase CTX-M-3. J. Antimicrob, Chemother","volume":"50","author":"L Poirel","year":"2002"},{"key":"ref47","first-page":"991","article-title":"Natural D240G Toho-1 mutant conferring resistance to ceftazidime: biochemical characterization of CTX-M-43. J. Antimicrob. Chemother","volume":"62","author":"G Celenza","year":"2008"},{"key":"ref48","first-page":"2269","article-title":"Novel cefotaximase (CTX-M-16) with increased catalytic efficiency due to substitution Asp240Gly. Antimicrob. Agents Chemother","volume":"45","author":"R Bonnet","year":"2001"},{"key":"ref49","first-page":"29","article-title":"Effect of D240G substitution in a novel ESBL CTX-M-27. J. Antimicrob. Chemother","volume":"52","author":"R Bonnet","year":"2003"},{"key":"ref50","first-page":"160","article-title":"Three decades of beta-lactamase inhibitors. Clin. Microbiol. Rev","volume":"23","author":"SM Drawz","year":"2010"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"1473","DOI":"10.1016\/S0140-6736(87)91180-9","article-title":"Resistance to \u03b2-lactam\/clavulanate","volume":"ii","author":"JL Martinez","year":"1987","journal-title":"Lancet"},{"key":"ref52","first-page":"595","article-title":"Small plasmids are involved in amoxicillin-clavulanate resistance in <italic>Escherichia coli<\/italic>. Antimicrob. Agents Chemother","volume":"33","author":"JL Martinez","year":"1989"},{"key":"ref53","first-page":"1365","article-title":"Resistance to ticarcillin-potassium clavulanate among clinical isolates of the family <italic>Enterobacteriaceae<\/italic>: role of PSE-1 \u03b2-lactamase and high levels of TEM-1 and SHV-1 and problems with false susceptibility in disk diffusion tests. Antimicrob. Agents Chemother","volume":"32","author":"CC Sanders","year":"1988"},{"key":"ref54","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1016\/S0140-6736(88)90391-1","article-title":"Amoxicillin\/clavulanate resistant <italic>Escherichia coli<\/italic>","volume":"i","author":"H Williams","year":"1988","journal-title":"Lancet"},{"key":"ref55","first-page":"494","article-title":"Effect of hyperproduction of TEM-1 \u03b2-lactamase on in vitro susceptibility of <italic>Escherichia coli<\/italic> to \u03b2-lactam antibiotics. Antimicrob. Agents Chemother","volume":"38","author":"PJ Wu","year":"1994"},{"key":"ref56","first-page":"927","article-title":"Mechanisms of hyperproduction of TEM-1 \u03b2-lactamase by clinical isolates of <italic>Escherichia coli<\/italic>. J. Antimicrob. Chemother","volume":"36","author":"PJ Wu","year":"1995"},{"key":"ref57","doi-asserted-by":"crossref","first-page":"700","DOI":"10.1038\/359700a0","article-title":"Molecular structure of the acyl-enzyme intermediate in \u03b2-lactam hydrolysis at 1.7 \u00c5 resolution","volume":"359","author":"NCJ Strynadka","year":"1992","journal-title":"Nature"}],"updated-by":[{"DOI":"10.1371\/annotation\/049bf1aa-d866-471f-95c1-5939d4461f8c","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2013,12,30]],"date-time":"2013-12-30T00:00:00Z","timestamp":1388361600000}}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0056926","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,7,10]],"date-time":"2019-07-10T00:49:02Z","timestamp":1562719742000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0056926"}},"subtitle":[],"editor":[{"given":"Hendrik W.","family":"van Veen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2013,2,21]]},"references-count":57,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2013,2,21]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0056926","relation":{"correction":[{"id-type":"doi","id":"10.1371\/annotation\/049bf1aa-d866-471f-95c1-5939d4461f8c","asserted-by":"object"}]},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,2,21]]}}}