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An individual-based modeling approach","volume":"25","author":"LJ Faust","year":"2006","journal-title":"Zoo Biology"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1111\/j.1469-1795.2008.00172.x","article-title":"Modeling population viability of captive elephants in Myanmar (Burma): implications for wild populations","volume":"11","author":"P Leimgruber","year":"2008","journal-title":"Animal Conservation"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"e20619","DOI":"10.1371\/journal.pone.0020619","article-title":"Will elephants soon disappear from West African savannahs","volume":"6","author":"P Bouch\u00e9","year":"2011","journal-title":"PLoS One"},{"key":"ref6","first-page":"41","article-title":"The human-elephant conflict: A review of current status and mitigation methods","volume":"30","author":"O Perera","year":"2009","journal-title":"Gajah"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1007\/s00267-011-9636-1","article-title":"Elephants also like coffee: Trends and drivers of human\u2013elephant conflicts in coffee agroforestry landscapes of Kodagu, Western Ghats, India","volume":"47","author":"P Bal","year":"2011","journal-title":"Environmental Management"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1111\/j.1469-7998.2008.00437.x","article-title":"Genetic diversity in the North American captive African elephant collection","volume":"275","author":"R Lei","year":"2008","journal-title":"Journal of Zoology"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/0011-2240(92)90003-K","article-title":"Potential contribution of cryopreserved germ plasm to the preservation of genetic diversity and conservation of endangered species in captivity","volume":"29","author":"JD Ballou","year":"1992","journal-title":"Cryobiology"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1016\/S0378-4320(98)00120-1","article-title":"Manual collection and characterization of semen from Asian elephants (<italic>Elephas maximus<\/italic>)","volume":"53","author":"DL Schmitt","year":"1998","journal-title":"Animal Reproduction Science"},{"key":"ref11","unstructured":"Hildebrandt TB, G\u00f6ritz F, Schnorrenberg A, Hermes R, Schmitt DL, <etal>et al<\/etal>. (1999) Successful artificial insemination of African nulliparous elephants at the Indianapolis Zoo; May 12\u201316; Vienna, Austria. Institut f\u00fcr Zoo- und Wildtierforschung. 41\u201346."},{"key":"ref12","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1002\/zoo.10116","article-title":"Successful Artificial Insemination of an Asian Elephant at the National Zoological Park","volume":"23","author":"JL Brown","year":"2004","journal-title":"Zoo Biology"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1038\/243038a0","article-title":"Collection, motility and storage of spermatozoa from the African elephant <italic>Loxodonta africana<\/italic>","volume":"243","author":"RC Jones","year":"1973","journal-title":"Nature"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1530\/jrf.0.0780295","article-title":"Influence of cryoprotective diluent on post-thaw viability and acrosomal integrity of spermatozoa of the African elephant (<italic>Loxodonta africana<\/italic>)","volume":"78","author":"JG Howard","year":"1986","journal-title":"Journal of Reproduction and Fertility"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/j.anireprosci.2008.11.010","article-title":"Successful cryopreservation of Asian elephant (<italic>Elephas maximus<\/italic>) spermatozoa","volume":"115","author":"J Saragusty","year":"2009","journal-title":"Animal Reproduction Science"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"748","DOI":"10.1016\/j.theriogenology.2003.11.021","article-title":"Evaluation of post-thaw Asian elephant (<italic>Elephas maximus<\/italic>) spermatozoa using flow cytometry: the effects of extender and cryoprotectant","volume":"62","author":"N Thongtip","year":"2004","journal-title":"Theriogenology"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"1134","DOI":"10.1071\/RD11266","article-title":"Pretreatment of Asian elephant (<italic>Elephas maximus<\/italic>) spermatozoa with cholesterol-loaded cyclodextrins and glycerol addition at 4\u00b0C improves cryosurvival","volume":"24","author":"WK Kiso","year":"2012","journal-title":"Reproduction, Fertility and Development"},{"key":"ref18","unstructured":"Olson D (2011) North American Region Studbook for the African Elephant (<italic>Loxodonta africana<\/italic>). Azle, Texas: Indianapolis Zoo. 144 p."},{"key":"ref19","unstructured":"Schwammer HM, Fruehwirth S (2011) African Elephant EEP (<italic>Loxodonta africana<\/italic>) European Studbook 2010. Vienna, Austria: Vienna Zoo. 48 p."},{"key":"ref20","unstructured":"Keele M, Lewis K, Dever K (2010) Asian Elephant (<italic>Elephas maximus<\/italic>) North American Regional Studbook. Portland, Oregon. 241 p."},{"key":"ref21","unstructured":"Belterman R (2011) European Regional EAZA - EEP Asian Elephant Studbook <italic>Elephas maximus<\/italic>. Rotterdam, Netherlands: Rotterdam Zoo. 34 p."},{"key":"ref22","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1002\/1098-2361(2000)19:5<447::AID-ZOO12>3.0.CO;2-#","article-title":"Social structure and helping behavior in captive elephants","volume":"19","author":"BA Schulte","year":"2000","journal-title":"Zoo Biology"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"779","DOI":"10.1016\/j.anbehav.2008.03.019","article-title":"Adolescence in male African elephants, <italic>Loxodonta africana<\/italic>, and the importance of sociality","volume":"76","author":"KE Evans","year":"2008","journal-title":"Animal Behaviour"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/0006-3207(84)90013-2","article-title":"Aspects of the ecology of the Asian elephant <italic>Elephas maximus<\/italic> L. in the Ruhuna National Park, Sri Lanka","volume":"29","author":"C Santiapillai","year":"1984","journal-title":"Biological Conservation"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1163\/156853986X00171","article-title":"Rutting behavior in African elephants: the phenomenon of musth","volume":"102","author":"JH Poole","year":"1987","journal-title":"Behaviour"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1016\/0003-3472(89)90014-6","article-title":"Announcing intent: the aggressive state of musth in African elephants","volume":"37","author":"JH Poole","year":"1989","journal-title":"Animal Behaviour"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"830","DOI":"10.1038\/292830a0","article-title":"Musth in the African elephant, <italic>Loxodonta africana<\/italic>","volume":"292","author":"JH Poole","year":"1981","journal-title":"Nature"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1163\/156853971X00087","article-title":"Reproductive behavior of the Asiatic elephant","volume":"38","author":"JF Eisenberg","year":"1971","journal-title":"Behaviour"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1016\/j.anbehav.2006.12.008","article-title":"Age, musth and paternity success in wild male African elephants, <italic>Loxodonta africana<\/italic>","volume":"74","author":"JA Hollister-Smith","year":"2007","journal-title":"Animal Behaviour"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1111\/j.1748-1090.2006.00020.x","article-title":"Aspects of the reproductive biology and breeding management of Asian and African elephants <italic>Elephas maximus<\/italic> and <italic>Loxodonta africana<\/italic>","volume":"40","author":"TB Hildebrandt","year":"2006","journal-title":"International Zoo Yearbook"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"140","DOI":"10.69649\/pachyderm.v35i1.1164","article-title":"Immobilizations and evaluation of clinical parameters from free-ranging elephants in southern Tanzania","volume":"35","author":"DG Mpanduji","year":"2003","journal-title":"Pachyderm"},{"key":"ref32","first-page":"9","article-title":"Semen preservation for reproduction management in rare and endangered species","volume":"33","author":"S Blottner","year":"1998","journal-title":"Advances in Ethology"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1016\/j.theriogenology.2008.10.008","article-title":"First successful artificial insemination with frozen-thawed semen in rhinoceros","volume":"71","author":"R Hermes","year":"2009","journal-title":"Theriogenology"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"1065","DOI":"10.1016\/j.theriogenology.2009.06.026","article-title":"Artificial insemination of captive European brown hares (<italic>Lepus europaeus<\/italic> PALLAS, 1778) with fresh and cryopreserved semen derived from free-ranging males","volume":"72","author":"TB Hildebrandt","year":"2009","journal-title":"Theriogenology"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"1256","DOI":"10.1016\/j.theriogenology.2010.05.031","article-title":"Cooling and freezing of epididymal sperm in the common hippopotamus (<italic>Hippopotamus amphibius<\/italic>)","volume":"74","author":"J Saragusty","year":"2010","journal-title":"Theriogenology"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1080\/10520290600931007","article-title":"Viability and acrosome staining of stallion spermatozoa by Chicago sky blue and Giemsa","volume":"81","author":"G K\u00fatv\u00f6lgyi","year":"2006","journal-title":"Biotechnic and Histochemistry"},{"key":"ref37","unstructured":"Howard JG, Marker L, Pukazhenthi BS, Roth TL, Swanson WF, <etal>et al<\/etal>. (2002) Genome resource banking and successful artificial insemination with cryopreserved sperm in the cheetah; October 6\u201311, 2002; Cape Town, Republic of South Africa. 70; Abstract PL15."},{"key":"ref38","doi-asserted-by":"crossref","first-page":"1398","DOI":"10.1016\/j.theriogenology.2012.06.014","article-title":"Enriching the captive elephant population genetic pool through artificial insemination with frozen-thawed semen collected in the wild","volume":"78","author":"TB Hildebrandt","year":"2012","journal-title":"Theriogenology"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1016\/j.theriogenology.2006.01.055","article-title":"Post-mortem semen cryopreservation and characterization in two different endangered gazelle species (<italic>Gazella gazella and Gazella dorcas<\/italic>) and one subspecies (<italic>Gazella gazelle acaiae<\/italic>)","volume":"66","author":"J Saragusty","year":"2006","journal-title":"Theriogenology"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1638\/2010-0186.1","article-title":"Attempted <italic>in vitro<\/italic> maturation and fertilization of postmortem Sumatran rhinoceros (<italic>Dicerorhinus sumatrensis<\/italic>) oocytes","volume":"42","author":"MA Stoops","year":"2011","journal-title":"Journal of Zoo and Wildlife Medicine"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"1258","DOI":"10.1016\/j.theriogenology.2011.05.032","article-title":"Gamete rescue in the African black rhinoceros (<italic>Diceros bicornis<\/italic>)","volume":"76","author":"MA Stoops","year":"2011","journal-title":"Theriogenology"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"1649","DOI":"10.1126\/science.1164298","article-title":"Compromised survivorship in zoo elephants","volume":"322","author":"R Clubb","year":"2008","journal-title":"Science"},{"key":"ref43","unstructured":"Hildebrandt TB, G\u00f6ritz F, Pratt NC, Lehnhardt JL, Brown JL, <etal>et al<\/etal>. (1998) Artificial insemination (AI) of an Asian elephant (<italic>Elephas maximus<\/italic>). In: Lauria A, Gandolfi F, Enne G, Gianaroli L, editors; 14\u201316 September, 1998; Milan, Italy. 546 (abstract)."},{"key":"ref44","doi-asserted-by":"crossref","first-page":"1294","DOI":"10.1095\/biolreprod61.5.1294","article-title":"Hormone secretion in the Asian elephant (<italic>Elephas maximus<\/italic>): characterization of ovulatory and anovulatory luteinizing hormone surges","volume":"61","author":"JL Brown","year":"1999","journal-title":"Biology of Reproduction"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"1147","DOI":"10.1095\/biolreprod55.5.1147","article-title":"Nonluteal estrous cycles of 3-week duration are initiated by anovulatory luteinizing hormone peaks in African elephants","volume":"55","author":"N Kapustin","year":"1996","journal-title":"Biology of Reproduction"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/S0378-4320(98)00123-7","article-title":"Endocrinology of the ovarian cycle and pregnancy in the Asian (<italic>Elephas maximus<\/italic>) and African (<italic>Loxodonta africana<\/italic>) elephant","volume":"53","author":"JK Hodges","year":"1998","journal-title":"Animal Reproduction Science"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1186\/1477-7827-6-9","article-title":"Potential factors affecting semen quality in the Asian elephant (<italic>Elephas maximus<\/italic>)","volume":"6","author":"N Thongtip","year":"2008","journal-title":"Reproductive Biology and Endocrinology"},{"key":"ref48","unstructured":"Laws RM (1969) Aspects of reproduction in the African elephant, <italic>Loxodonta africana<\/italic>. Journal of Reproduction and Fertility Supplement 6: 193\u2013217."},{"key":"ref49","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1111\/j.1469-7998.1973.tb04651.x","article-title":"Population dynamics of the African elephant (<italic>Loxodonta africana<\/italic>)","volume":"169","author":"J Hanks","year":"1973","journal-title":"Journal of Zoology"},{"key":"ref50","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1016\/0093-691X(95)00187-D","article-title":"Inverse relationship between testicular proliferation and apoptosis in mammalian seasonal breeders","volume":"44","author":"S Blottner","year":"1995","journal-title":"Theriogenology"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"1487","DOI":"10.1016\/S0093-691X(02)01201-3","article-title":"Seasonal timing of sperm production in roe deer: interrelationship among changes in ejaculate parameters, morphology and function of testis and accessory glands","volume":"59","author":"F G\u00f6ritz","year":"2003","journal-title":"Theriogenology"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0057616","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,29]],"date-time":"2025-04-29T23:23:42Z","timestamp":1745969022000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0057616"}},"subtitle":[],"editor":[{"given":"Nei","family":"Moreira","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2013,3,6]]},"references-count":51,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2013,3,6]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0057616","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,3,6]]}}}