{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T22:12:21Z","timestamp":1791843141828,"version":"4.3.4"},"reference-count":42,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2013,3,1]],"date-time":"2013-03-01T00:00:00Z","timestamp":1362096000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0058111","type":"journal-article","created":{"date-parts":[[2013,3,2]],"date-time":"2013-03-02T08:14:21Z","timestamp":1362212061000},"page":"e58111","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":48,"title":["Serologic Prevalence of Amoeba-Associated Microorganisms in Intensive Care Unit Pneumonia Patients"],"prefix":"10.1371","volume":"8","author":[{"given":"Sabri","family":"Bousbia","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurent","family":"Papazian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"Saux","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Marie","family":"Forel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Pierre","family":"Auffray","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claude","family":"Martin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Didier","family":"Raoult","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernard","family":"La Scola","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2013,3,1]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/0002-9343(80)90357-5","article-title":"Deaths from nosocomial infections: experience in a university hospital and a community hospital","volume":"68","author":"PA Gross","year":"1980","journal-title":"Am J Med"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1097\/00003246-199401000-00013","article-title":"Influence of nosocomial infection on mortality rate in an intensive care unit","volume":"22","author":"A Bueno-Cavanillas","year":"1994","journal-title":"Crit Care Med"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1001\/jama.1995.03530080055041","article-title":"The prevalence of nosocomial infection in intensive care units in Europe. Results of the European Prevalence of Infection in Intensive Care (EPIC) Study. EPIC International Advisory Committee","volume":"274","author":"JL Vincent","year":"1995","journal-title":"JAMA"},{"key":"ref4","first-page":"388","article-title":"Am J Respir Crit Care Med","volume":"171","year":"2005"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1111\/j.1469-0691.2008.02633.x","article-title":"Parachlamydia acanthamoebae, an emerging agent of pneumonia","volume":"15","author":"G Greub","year":"2009","journal-title":"Clin Microbiol Infect"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"702","DOI":"10.7326\/0003-4819-144-9-200605020-00025","article-title":"Laboratory infection of a technician by mimivirus","volume":"144","author":"D Raoult","year":"2006","journal-title":"Ann Intern Med"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"248","DOI":"10.3201\/eid1202.050434","article-title":"Ameba-associated microorganisms and diagnosis of nosocomial pneumonia","volume":"12","author":"P Berger","year":"2006","journal-title":"Emerg Infect Dis"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"449","DOI":"10.3201\/eid1103.040538","article-title":"Mimivirus in pneumonia patients","volume":"11","author":"B La Scola","year":"2005","journal-title":"Emerg Infect Dis"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"815","DOI":"10.3201\/eid0907.030065","article-title":"Amoeba-resisting bacteria and ventilator-associated pneumonia","volume":"9","author":"B La Scola","year":"2003","journal-title":"Emerg Infect Dis"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"625","DOI":"10.3201\/eid0806.010210","article-title":"Parachlamydiaceae: potential emerging pathogens","volume":"8","author":"G Greub","year":"2002","journal-title":"Emerg Infect Dis"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"1135","DOI":"10.1111\/j.1462-2920.2007.01530.x","article-title":"Isolation and identification of amoeba-resisting bacteria from water in human environment by using an Acanthamoeba polyphaga co-culture procedure","volume":"10","author":"I Pagnier","year":"2008","journal-title":"Environ Microbiol"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"2428","DOI":"10.1128\/AEM.72.4.2428-2438.2006","article-title":"Biodiversity of amoebae and amoeba-resisting bacteria in a hospital water network","volume":"72","author":"V Thomas","year":"2006","journal-title":"Appl Environ Microbiol"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"462","DOI":"10.1086\/502086","article-title":"Patients in the intensive care unit are exposed to amoeba-associated pathogens","volume":"23","author":"B La Scola","year":"2002","journal-title":"Infect Control Hosp Epidemiol"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1099\/ijs.0.02127-0","article-title":"Bosea eneae sp. nov., Bosea massiliensis sp. nov. and Bosea vestrisii sp. nov., isolated from hospital water supplies, and emendation of the genus Bosea (Das et al. 1996)","volume":"53","author":"B La Scola","year":"2003","journal-title":"Int J Syst Evol Microbiol"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1093\/clinchem\/48.1.121","article-title":"Antigen microarrays for serodiagnosis of infectious diseases","volume":"48","author":"L Mezzasoma","year":"2002","journal-title":"Clin Chem"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"530","DOI":"10.1196\/annals.1374.104","article-title":"Multiplexed serology in atypical bacterial pneumonia","volume":"1078","author":"F Gouriet","year":"2006","journal-title":"Ann N Y Acad Sci"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"1119","DOI":"10.1111\/j.1469-0691.2008.02119.x","article-title":"Comparison of the new InoDiag automated fluorescence multiplexed antigen microarray to the reference technique in the serodiagnosis of atypical bacterial pneumonia","volume":"14","author":"F Gouriet","year":"2008","journal-title":"Clin Microbiol Infect"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"1112","DOI":"10.1111\/j.1469-0691.2008.02094.x","article-title":"Multiplexed whole bacterial antigen microarray, a new format for the automation of serodiagnosis: the culture-negative endocarditis paradigm","volume":"14","author":"F Gouriet","year":"2008","journal-title":"Clin Microbiol Infect"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"959","DOI":"10.1007\/s10096-008-0528-0","article-title":"Serological microarray for a paradoxical diagnostic of Whipple\u2019s disease","volume":"27","author":"CJ Bonhomme","year":"2008","journal-title":"Eur J Clin Microbiol Infect Dis"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1186\/1471-2180-7-59","article-title":"Microarray for serotyping of Bartonella species","volume":"7","author":"CJ Bonhomme","year":"2007","journal-title":"BMC Microbiol"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1016\/j.jim.2008.12.003","article-title":"Development of a multiplexed fluorescent immunoassay for the quantitation of antibody responses to four Neisseria meningitidis serogroups","volume":"342","author":"TB Martins","year":"2009","journal-title":"J Immunol Methods"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1128\/CVI.00351-08","article-title":"Comparison of a multiplexed herpes simplex virus type-specific immunoglobulin G serology assay to immunoblot, Western blot, and enzyme-linked immunosorbent assays","volume":"16","author":"TB Martins","year":"2009","journal-title":"Clin Vaccine Immunol"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1128\/JCM.01867-06","article-title":"Serological cross-reactivity between different Chlamydia-like organisms","volume":"45","author":"N Casson","year":"2007","journal-title":"J Clin Microbiol"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"925","DOI":"10.1016\/S0140-6736(05)62701-8","article-title":"Chlamydia-like obligate parasite of free-living amoebae","volume":"349","author":"RJ Birtles","year":"1997","journal-title":"Lancet"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1111\/j.1749-6632.2003.tb07381.x","article-title":"Serological hint suggesting that Parachlamydiaceae are agents of pneumonia in polytraumatized intensive care patients","volume":"990","author":"G Greub","year":"2003","journal-title":"Ann N Y Acad Sci"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"578","DOI":"10.1164\/ajrccm.156.2.9608081","article-title":"Pneumonia with serological evidence of acute infection with the Chlamydia-like microorganism \u201cZ\u201d","volume":"156","author":"D Lieberman","year":"1997","journal-title":"Am J Respir Crit Care Med"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"1026","DOI":"10.3201\/eid0706.010619","article-title":"Legionella-like and other amoebal pathogens as agents of community-acquired pneumonia","volume":"7","author":"TJ Marrie","year":"2001","journal-title":"Emerg Infect Dis"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1159\/000312919","article-title":"Tentative characterization of new environmental giant viruses by MALDI-TOF mass spectrometry","volume":"53","author":"B La Scola","year":"2010","journal-title":"Intervirology"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1159\/000312915","article-title":"Advances in Mimivirus pathogenicity","volume":"53","author":"A Vincent","year":"2010","journal-title":"Intervirology"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1097\/CCM.0b013e318192fa8b","article-title":"Clinical significance of a positive serology for mimivirus in patients presenting a suspicion of ventilator-associated pneumonia","volume":"37","author":"A Vincent","year":"2009","journal-title":"Crit Care Med"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.micpath.2006.08.004","article-title":"Pneumonia in mice inoculated experimentally with Acanthamoeba polyphaga mimivirus","volume":"42","author":"M Khan","year":"2007","journal-title":"Microb Pathog"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/0923-2508(93)90213-L","article-title":"Balneatrix alpica gen. nov., sp. nov., a bacterium associated with pneumonia and meningitis in a spa therapy center","volume":"144","author":"C Dauga","year":"1993","journal-title":"Res Microbiol"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1007\/s10156-009-0692-X","article-title":"Prolonged Mycoplasma pneumoniae infection in an elderly patient with community-acquired pneumonia","volume":"15","author":"T Takahashi","year":"2009","journal-title":"J Infect Chemother"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"684","DOI":"10.1111\/j.1442-200X.2009.02858.x","article-title":"Prevalence of Mycoplasma IgM in children with lower respiratory tract illness","volume":"51","author":"M Higashigawa","year":"2009","journal-title":"Pediatr Int"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"665","DOI":"10.1056\/NEJM200103013440908","article-title":"Aspiration pneumonitis and aspiration pneumonia","volume":"344","author":"PE Marik","year":"2001","journal-title":"N Engl J Med"},{"key":"ref36","first-page":"S27","article-title":"Infectious Diseases Society of America\/American Thoracic Society consensus guidelines on the management of community-acquired pneumonia in adults","volume":"44","author":"LA Mandell","year":"2007","journal-title":"Bull Johns Hopkins Hosp"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1016\/j.jhin.2004.07.011","article-title":"Mycoplasma pneumoniae: a rarely diagnosed agent in ventilator-acquired pneumonia","volume":"59","author":"B La Scola","year":"2005","journal-title":"J Hosp Infect"},{"key":"ref38","first-page":"1773","article-title":"Description of Afipia birgiae sp. nov. and Afipia massiliensis sp. nov","volume":"52","author":"B La Scola","year":"2002","journal-title":"and recognition of Afipia felis genospecies A. Int J Syst Evol Microbiol"},{"key":"ref39","first-page":"374","article-title":"Biotechniques","volume":"34","year":"2003"},{"key":"ref40","doi-asserted-by":"crossref","unstructured":"Thomas V Perneger. What\u2019s wrong with Bonferroni adjustments (1998) British Medical Journal; Apr 18; 316, 7139.","DOI":"10.1136\/bmj.316.7139.1236"},{"key":"ref41","first-page":"1044","article-title":"Behav Ecol","volume":"15","year":"2004"},{"key":"ref42","doi-asserted-by":"crossref","unstructured":"Moran M.D (2003) Arguments for rejecting the sequential Bonferroni in ecological studies Oikos Volume 100, Issue 2, pages 403\u2013405.","DOI":"10.1034\/j.1600-0706.2003.12010.x"}],"updated-by":[{"DOI":"10.1371\/journal.pone.0278008","type":"expression_of_concern","label":"Expression of concern","source":"retraction-watch","updated":{"date-parts":[[2022,12,13]],"date-time":"2022-12-13T00:00:00Z","timestamp":1670889600000},"record-id":"45706"},{"DOI":"10.1371\/journal.pone.0278008","type":"expression_of_concern","label":"Expression of concern","source":"publisher","updated":{"date-parts":[[2022,12,13]],"date-time":"2022-12-13T00:00:00Z","timestamp":1670889600000}}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0058111","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,7,23]],"date-time":"2020-07-23T03:43:59Z","timestamp":1595475839000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0058111"}},"subtitle":[],"editor":[{"given":"Olivier","family":"Neyrolles","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2013,3,1]]},"references-count":42,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2013,3,1]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0058111","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,3,1]]}}}