{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T23:13:32Z","timestamp":1791242012938,"version":"4.1.0"},"reference-count":45,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2013,3,13]],"date-time":"2013-03-13T00:00:00Z","timestamp":1363132800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0058156","type":"journal-article","created":{"date-parts":[[2013,3,13]],"date-time":"2013-03-13T22:55:00Z","timestamp":1363215300000},"page":"e58156","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":33,"title":["Mcph1-Deficient Mice Reveal a Role for MCPH1 in Otitis Media"],"prefix":"10.1371","volume":"8","author":[{"given":"Jing","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neil","family":"Ingham","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon","family":"Clare","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claire","family":"Raisen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valerie E.","family":"Vancollie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ozama","family":"Ismail","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebecca E.","family":"McIntyre","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen H.","family":"Tsang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vinit B.","family":"Mahajan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gordon","family":"Dougan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David J.","family":"Adams","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacqueline K.","family":"White","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen P.","family":"Steel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2013,3,13]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"2125","DOI":"10.1001\/jama.282.22.2125","article-title":"The heritability of otitis media: a twin and triplet study","volume":"282","author":"ML Casselbrant","year":"1999","journal-title":"JAMA"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"624","DOI":"10.1177\/000348949710600802","article-title":"Distribution and heritability of recurrent ear infections","volume":"106","author":"KJ Kvaerner","year":"1997","journal-title":"Ann Otol Rhinol Laryngol"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"988","DOI":"10.1086\/426061","article-title":"Chronic and recurrent otitis media: a genome scan for susceptibility loci","volume":"75","author":"KA Daly","year":"2004","journal-title":"Am J Hum Genet"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1007\/s00335-010-9295-1","article-title":"Unraveling the genetics of otitis media: from mouse to human and back again","volume":"22","author":"MS Rye","year":"2011","journal-title":"Mamm Genome"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1038\/nature10163","article-title":"A conditional knockout resource for the genome-wide study of mouse gene function","volume":"474","author":"WC Skarnes","year":"2011","journal-title":"Nature"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1086\/301966","article-title":"Primary autosomal recessive microcephaly (MCPH1) maps to chromosome 8p22-pter","volume":"63","author":"AP Jackson","year":"1998","journal-title":"Am J Hum Genet"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"717","DOI":"10.1086\/429930","article-title":"Autosomal Recessive Primary Microcephaly (MCPH): A Review of Clinical, Molecular, and Evolutionary Findings","volume":"76","author":"C Woods","year":"2005","journal-title":"The American Journal of Human Genetics"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"15105","DOI":"10.1073\/pnas.0507722102","article-title":"BRIT1\/MCPH1 is a DNA damage responsive protein that regulates the Brca1\u2013Chk1 pathway, implicating checkpoint dysfunction in microcephaly","volume":"102","author":"SY Lin","year":"2005","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"e1000826","DOI":"10.1371\/journal.pgen.1000826","article-title":"BRIT1\/MCPH1 is essential for mitotic and meiotic recombination DNA repair and maintaining genomic stability in mice","volume":"6","author":"Y Liang","year":"2010","journal-title":"PLoS Genet"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/j.ccr.2006.07.002","article-title":"BRIT1 regulates early DNA damage response, chromosomal integrity, and cancer","volume":"10","author":"R Rai","year":"2006","journal-title":"Cancer Cell"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1038\/ncb1431","article-title":"Regulation of mitotic entry by microcephalin and its overlap with ATR signalling","volume":"8","author":"GK Alderton","year":"2006","journal-title":"Nat Cell Biol"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"29586","DOI":"10.1074\/jbc.M804080200","article-title":"Microcephalin\/MCPH1 associates with the Condensin II complex to function in homologous recombination repair","volume":"283","author":"JL Wood","year":"2008","journal-title":"J Biol Chem"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"1325","DOI":"10.1038\/ncb2342","article-title":"MC1 regulates the neuroprogenitor division mode by coupling the centrosomal cycle with mitotic entry through the Chk1-Cdc25 pathway","volume":"13","author":"R Gruber","year":"2011","journal-title":"Nat Cell Biol"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method","volume":"25","author":"KJ Livak","year":"2001","journal-title":"Methods"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1038\/ng0992-75","article-title":"Normal hearing in Splotch (Sp\/+), the mouse homologue of Waardenburg syndrome type 1","volume":"2","author":"KP Steel","year":"1992","journal-title":"Nat Genet"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"3","DOI":"10.3109\/00016487809122718","article-title":"A technique for preparation of cochlear specimens for assessment with the scanning electron microscope","volume":"351","author":"IM Hunter-Duvar","year":"1978","journal-title":"Acta Otolaryngol Suppl"},{"key":"ref17","doi-asserted-by":"crossref","unstructured":"Shima N, Hartford SA, Duffy T, Wilson LA, Schimenti KJ, et al.. (2003) Phenotype-based identification of mouse chromosome instability mutants. Genetics 163:: 1031\u20131040.","DOI":"10.1093\/genetics\/163.3.1031"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"2258","DOI":"10.1128\/IAI.58.7.2258-2261.1990","article-title":"Moderate immunodeficiency does not increase susceptibility to Salmonella typhimurium aroA live vaccines in mice","volume":"58","author":"M Izhar","year":"1990","journal-title":"Infect Immun"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"963","DOI":"10.1111\/j.1462-5822.2004.00414.x","article-title":"Organ specificity, colonization and clearance dynamics in vivo following oral challenges with the murine pathogen Citrobacter rodentium","volume":"6","author":"S Wiles","year":"2004","journal-title":"Cell Microbiol"},{"key":"ref20","article-title":"Mouse eye enucleation for remote high-throughput phenotyping","author":"VB Mahajan","year":"2011","journal-title":"J Vis Exp"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"e149","DOI":"10.1371\/journal.pgen.0020149","article-title":"Mutation at the Evi1 locus in Junbo mice causes susceptibility to otitis media","volume":"2","author":"N Parkinson","year":"2006","journal-title":"PLoS Genet"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"2435","DOI":"10.1038\/jid.2009.104","article-title":"Matriptase-deficient mice exhibit ichthyotic skin with a selective shift in skin microbiota","volume":"129","author":"TC Scharschmidt","year":"2009","journal-title":"J Invest Dermatol"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.heares.2006.05.012","article-title":"Evaluation of the mouse model for acute otitis media","volume":"219","author":"CJ MacArthur","year":"2006","journal-title":"Hear Res"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1080\/000164801316878863","article-title":"The use of Preyer&apos;s reflex in evaluation of hearing in mice","volume":"121","author":"J Jero","year":"2001","journal-title":"Acta Otolaryngol"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1177\/000348940211100311","article-title":"Auditory consequences of recurrent acute purulent otitis media","volume":"111","author":"M Ryding","year":"2002","journal-title":"Ann Otol Rhinol Laryngol"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"193","DOI":"10.3109\/00016489509139290","article-title":"Otitis media and long-term follow-up of hearing","volume":"115","author":"M Sorri","year":"1995","journal-title":"Acta Otolaryngol"},{"key":"ref27","first-page":"100","article-title":"Endotoxin permeability through the round window","volume":"457","author":"H Kawauchi","year":"1989","journal-title":"Acta Otolaryngol Suppl"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"980","DOI":"10.1177\/000348948909801212","article-title":"Round window membrane and perilymph in experimental otitis media with effusion","volume":"98","author":"M Suzuki","year":"1989","journal-title":"Ann Otol Rhinol Laryngol"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1288\/00005537-199202000-00013","article-title":"Influence of middle ear immune response on the immunological state and function of the inner ear","volume":"102","author":"B Gloddek","year":"1992","journal-title":"Laryngoscope"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1111\/j.1749-6632.1997.tb51885.x","article-title":"Effects of inflammatory mediators on middle ear pathology and on inner ear function","volume":"830","author":"SK Juhn","year":"1997","journal-title":"Ann N Y Acad Sci"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/S0378-5955(99)00134-3","article-title":"Effect of proinflammatory cytokines on cultured spiral ligament fibrocytes","volume":"137","author":"K Yoshida","year":"1999","journal-title":"Hear Res"},{"key":"ref32","first-page":"651","article-title":"Eya4-deficient mice are a model for heritable otitis media","volume":"118","author":"FF Depreux","year":"2008","journal-title":"J Clin Invest"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/S0165-5876(02)00070-8","article-title":"Anomaly of the eustachian tube and its associated structures in patients with multiple congenital malformation: a histopathological and morphometric study","volume":"64","author":"M Miura","year":"2002","journal-title":"Int J Pediatr Otorhinolaryngol"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1177\/03946320110240S401","article-title":"Adenoids during childhood: the facts","volume":"24","author":"GL Marseglia","year":"2011","journal-title":"Int J Immunopathol Pharmacol"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1007\/s00330-011-2303-3","article-title":"Functional MR imaging of the eustachian tube in patients with clinically proven dysfunction: correlation with lesions detected on MR images","volume":"22","author":"A Lukens","year":"2012","journal-title":"Eur Radiol"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1083\/jcb.200809144","article-title":"Inactivation of Chibby affects function of motile airway cilia","volume":"185","author":"VA Voronina","year":"2009","journal-title":"J Cell Biol"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1093\/hmg\/11.6.715","article-title":"Loss of function of axonemal dynein Mdnah5 causes primary ciliary dyskinesia and hydrocephalus","volume":"11","author":"I Ibanez-Tallon","year":"2002","journal-title":"Hum Mol Genet"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"1539","DOI":"10.1097\/00005537-199810000-00021","article-title":"Classification of otitis media","volume":"108","author":"P Harkness","year":"1998","journal-title":"Laryngoscope"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1046\/j.1365-2273.2000.00350.x","article-title":"The aetiology of otitis media with effusion: a review","volume":"25","author":"H Kubba","year":"2000","journal-title":"Clin Otolaryngol Allied Sci"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1016\/j.tig.2009.09.011","article-title":"Primary microcephaly: do all roads lead to Rome?","volume":"25","author":"GK Thornton","year":"2009","journal-title":"Trends Genet"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"273","DOI":"10.3109\/00016488509104790","article-title":"Ultrastructure of middle ear mucosa in the Mongolian gerbil, Meriones unguiculatus","volume":"100","author":"RA Chole","year":"1985","journal-title":"Acta Otolaryngol"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"19","DOI":"10.3109\/00016488409122876","article-title":"Ultrastructure of the middle ear mucosa","volume":"414","author":"E Hentzer","year":"1984","journal-title":"Acta Otolaryngol Suppl"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1038\/sj.onc.1210595","article-title":"Distinct BRCT domains in Mcph1\/Brit1 mediate ionizing radiation-induced focus formation and centrosomal localization","volume":"27","author":"LJ Jeffers","year":"2008","journal-title":"Oncogene"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"e9242","DOI":"10.1371\/journal.pone.0009242","article-title":"Establishment of a mouse model with misregulated chromosome condensation due to defective Mcph1 function","volume":"5","author":"M Trimborn","year":"2010","journal-title":"PLoS ONE"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"R90","DOI":"10.1186\/gb-2011-12-9-r90","article-title":"Exome sequencing identifies a missense mutation in Isl1 associated with low penetrance otitis media in dearisch mice","volume":"12","author":"JM Hilton","year":"2011","journal-title":"Genome Biol"}],"updated-by":[{"DOI":"10.1371\/annotation\/fadb7426-df05-4ec5-a0ba-21981295b0eb","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2013,6,19]],"date-time":"2013-06-19T00:00:00Z","timestamp":1371600000000}}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0058156","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,11]],"date-time":"2022-02-11T11:12:48Z","timestamp":1644577968000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0058156"}},"subtitle":[],"editor":[{"given":"Patrick","family":"Callaerts","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2013,3,13]]},"references-count":45,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2013,3,13]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0058156","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,3,13]]}}}