{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,9]],"date-time":"2025-04-09T05:45:51Z","timestamp":1744177551388},"reference-count":57,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2013,3,5]],"date-time":"2013-03-05T00:00:00Z","timestamp":1362441600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0058357","type":"journal-article","created":{"date-parts":[[2013,3,5]],"date-time":"2013-03-05T22:30:10Z","timestamp":1362522610000},"page":"e58357","update-policy":"http:\/\/dx.doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":19,"title":["The Synergistic Effect of Combined Immunization with a DNA Vaccine and Chimeric Yellow Fever\/Dengue Virus Leads to Strong Protection against Dengue"],"prefix":"10.1371","volume":"8","author":[{"given":"Adriana S.","family":"Azevedo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ant\u00f4nio J. S.","family":"Gon\u00e7alves","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcia","family":"Archer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcos S.","family":"Freire","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ricardo","family":"Galler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ada M. B.","family":"Alves","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2013,3,5]]},"reference":[{"key":"ref1","first-page":"330","article-title":"The global emergence\/resurgence of arboviral diseases as public health problems. Arch Med Res","volume":"33","author":"DJ Gubler","year":"2002"},{"key":"ref2","first-page":"33","article-title":"Dengue: an update. The Lancet Infection Disease","volume":"2","author":"MG Guzm\u00e1n","year":"2002"},{"key":"ref3","first-page":"105","article-title":"Important advances in the field of anti-dengue virus research. Antivir Chem Chemother","volume":"21","author":"JG Julander","year":"2011"},{"key":"ref4","unstructured":"Monath TP, Tsai TF (1997) Flaviviruses. In: Richman DD, Whitley RJ, and Hayden FG, editors. Clinical Virology. New York: Churchill Livingstone Inc. 1133\u20131179."},{"key":"ref5","unstructured":"Lindenbach BD, Rice CM (2001) Flaviviridae: the viruses and their replication. In: Knipe DM, Howley PM editors. Fields' Virology. Philadelphia: Lippincot Williams & Wilkins. 991\u20131042."},{"key":"ref6","first-page":"717","article-title":"Structure of dengue virus: implications for flavivirus organization, maturation and fusion. Cell","volume":"108","author":"RJ Kuhn","year":"2002"},{"key":"ref7","first-page":"e1000823","article-title":"The development of therapeutic antibodies that neutralize homologous and heterologous genotypes of dengue virus type 1. PLoS Pathog","volume":"6","author":"B Shrestha","year":"2010"},{"key":"ref8","first-page":"518","article-title":"Prospects for a dengue virus vaccine. Nat Rev Microbiol","volume":"5","author":"SS Whitehead","year":"2007"},{"key":"ref9","first-page":"587","article-title":"Immune response to dengue virus and prospects for a vaccine. Annu Rev Immunol","volume":"29","author":"BR Murphy","year":"2011"},{"key":"ref10","first-page":"239","article-title":"Review of dengue virus and the development of a vaccine. Biotechnol Adv","volume":"29","author":"S Murrell","year":"2011"},{"key":"ref11","first-page":"532","article-title":"Immunity to dengue virus: a tale of original antigenic sin and tropical cytokine storms. Nat Rev Immunol","volume":"11","author":"AL Rothman","year":"2011"},{"key":"ref12","first-page":"2705","article-title":"Development of a recombinant tetravalent dengue virus vaccine: immunogenicity and efficacy studies in mice and monkeys. Vaccine","volume":"28","author":"DE Clements","year":"2010"},{"key":"ref13","first-page":"56","article-title":"Dengue vaccines approach the finish line. ClinInfectDis","volume":"45","author":"R Edelman","year":"2007"},{"key":"ref14","first-page":"7229","article-title":"From research to phase III: Preclinical, industrial and clinical development of the Sanofi Pasteur tetravalent dengue vaccine. Vaccine","volume":"29","author":"B Guy","year":"2011"},{"key":"ref15","first-page":"632","article-title":"Preclinical and clinical development of YFV 17D-based chimeric vaccines against dengue, West Nile and Japanese encephalitis viruses. Vaccine","volume":"28","author":"B Guy","year":"2010"},{"key":"ref16","first-page":"2200","article-title":"Dengue tetravalent DNA vaccine inducing neutralizing antibody and anamnestic responses to four serotypes in mice. Vaccine","volume":"24","author":"E Konishi","year":"2006"},{"key":"ref17","first-page":"11634","article-title":"A heterologous DNA prime-Venezuelan equine encephalitis virus replicon particle boost dengue vaccine regimen affords complete protection from virus challenge in cynomolgus macaques. J Virol","volume":"81","author":"L Chen","year":"2007"},{"key":"ref18","first-page":"268","article-title":"Distinct Humoral and Cellular Immunity Induced by Alternating Prime-boost Vaccination Using Plasmid DNA and Live Viral Vector Vaccines Expressing the E Protein of Dengue Virus Type 2. Immune Netw","volume":"11","author":"JA George","year":"2011"},{"key":"ref19","first-page":"2120","article-title":"Heterologous plasmid DNA prime-recombinant human adenovirus 5 boost vaccination generates a stable pool of protective long-lived CD8(+) T effector memory cells specific for a human parasite, Trypanosoma cruzi. Infect Immun","volume":"79","author":"PO Rigato","year":"2011"},{"key":"ref20","first-page":"e20528","article-title":"DNA vaccines against dengue virus type 2 based on truncate envelope protein or its domain III. PLoS One","volume":"6","author":"AS Azevedo","year":"2011"},{"key":"ref21","first-page":"1","article-title":"Construction, characterization and immunogenicity of recombinant yellow fever 17D-dengue type 2 viruses. Virus Res","volume":"79","author":"PS Caufour","year":"2001"},{"key":"ref22","first-page":"195","article-title":"Protection against dengue type 2 virus induced in mice immunized with a DNA plasmid encoding the non-structural 1 (NS1) gene fused to the tissue plasminogen activator signal sequence. Vaccine","volume":"24","author":"SM Costa","year":"2006"},{"key":"ref23","first-page":"6540","article-title":"Multiple specificities in the murine CD4+ and CD8+ T-cell response to dengue virus. J Virol","volume":"70","author":"AL Rothman","year":"1996"},{"key":"ref24","first-page":"e25685","article-title":"Induction of a protective response in mice by the dengue virus NS3 protein using DNA vaccines. PLoS One","volume":"6","author":"SM Costa","year":"2011"},{"key":"ref25","first-page":"159","article-title":"A comparative study with various anesthetics in mice (pentobarbitone, ketamine-xylazine, carfentanyl-etomidate). Res Exp Med","volume":"184","author":"W Erhardt","year":"1984"},{"key":"ref26","first-page":"1897","article-title":"Influence of cellular location of expressed antigen on the efficacy of DNA vaccination: cytotoxic T lymphocyte and antibody responses are suboptimal when antigen is cytoplasmic after intramuscular DNA immunization. Int Immunol","volume":"9","author":"JS Boyle","year":"1997"},{"key":"ref27","first-page":"413","article-title":"DNA vaccines against dengue virus based on the ns1 gene: the influence of different signal sequences on the protein expression and its correlation to the immune response elicited in mice. Virology","volume":"358","author":"SM Costa","year":"2007"},{"key":"ref28","first-page":"185","article-title":"Plasmid DNA expressing a secreted or a nonsecreted form of hepatitis C virus nucleocapsid: comparative studies of antibody and T-helper responses following genetic immunization. DNA Cell Biol","volume":"16","author":"G Inchauspe","year":"1997"},{"key":"ref29","first-page":"1835","article-title":"Attenuation and immunogenicity of recombinant yellow fever 17D-dengue type 2 virus for rhesus monkeys. Braz J Med Biol Res","volume":"38","author":"R Galler","year":"2005"},{"key":"ref30","first-page":"162","article-title":"Epitopic analysis of antigenic determinants on the surface of dengue-2 virions using monoclonal antibodies.Am J Trop Med Hyg","volume":"34","author":"EA Henchal","year":"1985"},{"key":"ref31","first-page":"1223","article-title":"Variable surface epitopes in the crystal structure of dengue virus type 3 envelope glycoprotein. J Virol","volume":"79","author":"Y Modis","year":"2005"},{"key":"ref32","first-page":"317","article-title":"Monoclonal antibody mapping of the envelope glycoprotein of the dengue 2 virus. Virology","volume":"246","author":"JT Roehrig","year":"1998"},{"key":"ref33","first-page":"12816","article-title":"Type- and subcomplex-specific neutralizing antibodies against domain III of dengue virus type 2 envelope protein recognize adjacent epitopes. J Virol","volume":"81","author":"S Sukupolvi-Petty","year":"2007"},{"key":"ref34","first-page":"103","article-title":"Dengue virus neutralization by human immune sera: role of envelope protein domain III-reactive antibody. Virology","volume":"392","author":"WM Wahala","year":"2009"},{"key":"ref35","first-page":"1076","article-title":"Dengue tetravalent DNA vaccine increases its immunogenicity in mice when mixed with a dengue type 2 subunit vaccine or an inactivated Japanese encephalitis vaccine. Vaccine","volume":"25","author":"J Imoto","year":"2007"},{"key":"ref36","first-page":"10","article-title":"An adenovirus prime\/plasmid boost strategy for induction of equipotent immune responses to two dengue virus serotypes. BMC Biotechnol","volume":"7","author":"S Khanam","year":"2007"},{"key":"ref37","first-page":"1826","article-title":"Simultaneous immunization with DNA and protein vaccines against Japanese encephalitis or dengue synergistically increases their own abilities to induce neutralizing antibody in mice. Vaccine","volume":"21","author":"E Konishi","year":"2003"},{"key":"ref38","first-page":"420","article-title":"Characterization of antibody responses to combinations of a dengue-2 DNA and dengue-2 recombinant subunit vaccine. Am J Trop Med Hyg","volume":"65","author":"M Simmons","year":"2001"},{"key":"ref39","first-page":"9577","article-title":"Characterization of antibody responses to combinations of a dengue virus type 2 DNA vaccine and two dengue virus type 2 protein vaccines in rhesus macaques. J Virol","volume":"80","author":"M Simmons","year":"2006"},{"key":"ref40","first-page":"2339","article-title":"Immunogenicities of Env glycoproteins from circulating HIV-1 isolates in China focusing on the strategy of "DNA prime plus protein boost". Chin Med J","volume":"122","author":"Z Wang","year":"2009"},{"key":"ref41","first-page":"1917","article-title":"Preexposure efficacy of a novel combination DNA and inactivated rabies virus vaccine. Hum Gene Ther","volume":"12","author":"S Biswas","year":"2001"},{"key":"ref42","first-page":"3895","article-title":"A human challenge model for dengue infection reveals a possible protective role for sustained interferon gamma levels during the acute phase of illness. Vaccine","volume":"29","author":"VJ Gunther","year":"2011"},{"key":"ref43","first-page":"1855","article-title":"Both CXCR3 and CXCL10\/IFN-inducible protein 10 are required for resistance to primary infection by dengue virus. J Immunol","volume":"177","author":"MF Hsieh","year":"2006"},{"key":"ref44","first-page":"2701","article-title":"Interferon-dependent immunity is essential for resistance to primary dengue virus infection in mice, whereas T- and B-cell-dependent immunity are less critical. J Virol","volume":"78","author":"S Shresta","year":"2004"},{"key":"ref45","first-page":"4865","article-title":"A protective role for dengue virus-specific CD8+ T cells. J Immunol","volume":"182","author":"LE Yauch","year":"2009"},{"key":"ref46","first-page":"62","article-title":"DNA vaccines: an historical perspective and view to the future. Immunol Rev","volume":"239","author":"MA Liu","year":"2011"},{"key":"ref47","first-page":"1745","article-title":"Heterologous protection against influenza by injection of DNA encoding a viral protein. Science","volume":"259","author":"JB Ulmer","year":"1993"},{"key":"ref48","first-page":"11468","article-title":"The HIV\/AIDS vaccine candidate MVA-B administered as a single immunogen in humans triggers robust, polyfunctional, and selective effector memory T cell responses to HIV-1 antigens. J Virol","volume":"85","author":"CE G\u00f3mez","year":"2011"},{"key":"ref49","first-page":"7506","article-title":"Robust Recall and Long-Term Memory T-Cell Responses Induced by Prime-Boost Regimens with Heterologous Live Viral Vectors Expressing Human Immunodeficiency Virus Type 1 Gag and Env Proteins. J Virol","volume":"76","author":"K Haglund","year":"2002"},{"key":"ref50","first-page":"340","article-title":"Insights into human CD8+ T-cell memory using the yellow fever and smallpox vaccines Immunology and Cell Biology","volume":"89","author":"R Ahmed","year":"2011"},{"key":"ref51","first-page":"1559","article-title":"Protective efficacy of the recombinant, live-attenuated, CYD tetravalent dengue vaccine in Thai schoolchildren: a randomised, controlled phase 2b trial. Lancet","volume":"380","author":"A Sabchareon","year":"2012"},{"key":"ref52","first-page":"204","article-title":"Retrospective study on dengue fatal cases. Clin Neuropathol","volume":"16","author":"MP Miagostovich","year":"1997"},{"key":"ref53","first-page":"465","article-title":"Dengue v\u00edrus in the brain of a fatal case of hemorrhagic dengue fever. J Neurovirol","volume":"4","author":"C Ramos","year":"1998"},{"key":"ref54","first-page":"75","article-title":"Review of the etiologies of viral meningitis and encephalitis in a dengue endemic region. J Neurol Sci","volume":"303","author":"CN Soares","year":"2011"},{"key":"ref55","first-page":"997","article-title":"Protectiveefficacy of a dengue 2 DNA vaccine in mice and the effect of CpG immunostimulatory motifs on antibody responses. Arch Virol","volume":"143","author":"KR Porter","year":"1998"},{"key":"ref56","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1016\/j.virol.2009.08.029","article-title":"A novel fusion protein domain III-capsid from dengue-2, in a highly aggregated form, induces a functional immune response and protection in mice","volume":"394","author":"I Vald\u00e9s","year":"2009","journal-title":"Virology"},{"key":"ref57","first-page":"7290","article-title":"Construction, safety, and immunogenicity in nonhuman primates of a chimeric yellow fever-dengue virus tetravalent vaccine. J Virol","volume":"75","author":"F Guirakhoo","year":"2001"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0058357","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2018,10,23]],"date-time":"2018-10-23T06:34:04Z","timestamp":1540276444000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0058357"}},"subtitle":[],"editor":[{"given":"Anil Kumar","family":"Tyagi","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2013,3,5]]},"references-count":57,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2013,3,5]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0058357","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,3,5]]}}}