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(2011) Family Rhabdoviridae. In: King AMQ, Adams MJ, Carstens EB, et al. editors Virus taxonomy: classification and nomenclature of viruses. Ninth Report of the International Committee on Taxonomy of Viruses San Diego: Elsevier."},{"key":"ref2","first-page":"1","article-title":"Expert Consultation on Rabies, First report. World Health Organ. Tech. Rep. Ser","volume":"931","year":"2005"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1111\/j.1439-0450.2004.00793.x","article-title":"Rabies in red foxes (Vulpes vulpes) experimentally infected with European bat lyssavirus type 1","volume":"51(7)","author":"A Vos","year":"2004","journal-title":"J Vet Med B Infect Dis Vet Public Health"},{"key":"ref4","first-page":"28","article-title":"Phylogenetic relationships of Irkut and West Caucasian bat viruses within the Lyssavirus genus and suggested quantitative criteria based on the N gene sequence for lyssavirus genotype definition. Virus Res","volume":"111(1)","author":"IV Kuzmin","year":"2005"},{"key":"ref5","first-page":"1519","article-title":"Novel Lyssavirus in Natterer\u2019s Bat, Germany. Emerg Infect Dis","volume":"17(8)","author":"CM Freuling","year":"2011"},{"key":"ref6","unstructured":"Dean DJ, Abelseth MK, Athanasiu P (1996) The fluorescence antibody test In: Meslin FX, Kaplan MM, Koprowski H editors Laboratory techniques in rabies Geneva: World Health Organization. 88\u201393."},{"key":"ref7","unstructured":"Office International des Epizooties (2008) Rabies. OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals Paris: OIE. 307\u2013308."},{"key":"ref8","doi-asserted-by":"crossref","first-page":"e765","DOI":"10.1371\/journal.pntd.0000765","article-title":"More accurate insight into the incidence of human rabies in developing countries through validated laboratory techniques","volume":"4","author":"L Dacheux","year":"2010","journal-title":"PLoS Negl Trop Dis"},{"key":"ref9","first-page":"1117","article-title":"Intravitam diagnosis of human rabies by PCR using saliva and cerebrospinal fluid. J Clin Microbiol","volume":"36(4)","author":"P Crepin","year":"1998"},{"key":"ref10","first-page":"111","article-title":"Rabies virus detection by RT-PCR in decomposed naturally infected brains. Vet Microbiol","volume":"87(2)","author":"D David","year":"2002"},{"key":"ref11","first-page":"912","article-title":"Rabies virus in the decomposed brain of an Ethiopian wolf detected by nested reverse transcription-polymerase chain reaction. J Wildl Dis","volume":"33(4)","author":"JE Whitby","year":"1997"},{"key":"ref12","first-page":"2762","article-title":"Heminested PCR assay for detection of six genotypes of rabies and rabies-related viruses. J Clin Microbiol","volume":"35(11)","author":"PR Heaton","year":"1997"},{"key":"ref13","first-page":"3678","article-title":"Screening of active lyssavirus infection in wild bat populations by viral RNA detection on oropharyngeal swabs. J Clin Microbiol","volume":"39(10)","author":"JE Echevarria","year":"2001"},{"key":"ref14","first-page":"281","article-title":"RT-PCR for detection of all seven genotypes of Lyssavirus genus. J Virol Methods","volume":"135(2)","author":"S Vazquez-Moron","year":"2006"},{"key":"ref15","first-page":"2786","article-title":"Development of a real-time, TaqMan reverse transcription-PCR assay for detection and differentiation of lyssavirus genotypes 1, 5, and 6. J Clin Microbiol","volume":"43(6)","author":"PR Wakeley","year":"2005"},{"key":"ref16","first-page":"1484","article-title":"Development of real-time reverse transcriptase polymerase chain reaction methods form human rabies diagnosis. J. Med. Virol","volume":"84","author":"SA Nadin-Davis","year":"2009"},{"key":"ref17","first-page":"3970","article-title":"Improved safety for molecular diagnosis of classical rabies viruses by use of a TaqMan real-time reverse transcription-PCR \u201cdouble check\u201d strategy. J Clin Microbiol","volume":"48(11)","author":"B Hoffmann","year":"2010"},{"key":"ref18","doi-asserted-by":"crossref","unstructured":"Hayman DT, Banyard AC, Wakeley PR, Harkess G, Marston D, <etal>et al<\/etal>.. (2011) A universal real-time assay for the detection of Lyssaviruses. J Virol Methods.","DOI":"10.1016\/j.jviromet.2011.07.002"},{"key":"ref19","unstructured":"Fooks AR, McElhinney LM, Horton D, Banyard A, Johnson N, <etal>et al<\/etal>.. (2012): Compendium of the OIE Global Conference on Rabies Control. in press."},{"key":"ref20","first-page":"15","article-title":"International interlaboratory trials on rabies diagnosis: an overview of results and variation in reference diagnosis techniques (fluorescent antibody test, rabies tissue culture infection test, mouse inoculation test) and molecular biology techniques. J Virol Methods","volume":"177(1)","author":"E Robardet","year":"2011"},{"key":"ref21","first-page":"200","article-title":"A universal heterologous internal control system for duplex real-time RT-PCR assays used in a detection system for pestiviruses. J Virol Methods","volume":"136(1\u20132)","author":"B Hoffmann","year":"2006"},{"key":"ref22","first-page":"313","article-title":"Comparison of real-time PCR and heminested RT-PCR methods in the detection of rabies virus infection in bats and terrestrial animals. B Vet I Pulawy","volume":"52(3)","author":"A Orlowska","year":"2008"},{"key":"ref23","first-page":"87","article-title":"A universal real-time assay for the detection of Lyssaviruses. J Virol Methods","volume":"177(1)","author":"DT Hayman","year":"2011"},{"key":"ref24","first-page":"15026","article-title":"Regular exposure to rabies virus and lack of symptomatic disease in Serengeti spotted hyenas. Proc Natl Acad Sci U S A","volume":"98(26)","author":"ML East","year":"2001"},{"key":"ref25","first-page":"1309","article-title":"Detection of rabies virus RNA isolated from several species of animals in Brazil by RT-PCR. The Journal of veterinary medical science\/the Japanese Society of Veterinary Science","volume":"63(12)","author":"M Ito","year":"2001"},{"key":"ref26","first-page":"49","article-title":"Spill-over of European bat lyssavirus type 1 into a stone marten (Martes foina) in Germany. J Vet Med B Infect Dis Vet Public Health","volume":"51(2)","author":"T M\u00fcller","year":"2004"},{"key":"ref27","first-page":"55","article-title":"Susceptibility of ferrets (Mustela putorius furo) to experimentally induced rabies with European Bat Lyssaviruses (EBLV). J Vet Med B Infect Dis Vet Public Health","volume":"51(2)","author":"A Vos","year":"2004"},{"key":"ref28","first-page":"999","article-title":"Classical swine fever virus detection: results of a real-time reverse transcription polymerase chain reaction ring trial conducted in the framework of the European network of excellence for epizootic disease diagnosis and control. J Vet Diagn Invest","volume":"23(5)","author":"B Hoffmann","year":"2011"},{"key":"ref29","first-page":"264","article-title":"Perspectives on molecular detection methods of lyssaviruses. Berl Munch Tierarztl Wochenschr","volume":"125(5\u20136)","author":"M Fischer","year":"2012"},{"key":"ref30","first-page":"299","article-title":"Evaluation of a TaqMan PCR assay to detect rabies virus RNA: influence of sequence variation and application to quantification of viral loads. J Clin Microbiol","volume":"42(1)","author":"GJ Hughes","year":"2004"},{"key":"ref31","first-page":"403","article-title":"Rabies in a captive colony of big brown bats (Eptesicus Fuscus). J. Wildl. Dis","volume":"40(3)","author":"V Shankar","year":"2004"},{"key":"ref32","first-page":"837","article-title":"Role of reverse transcriptase polymerase chain reaction for the diagnosis of human rabies. The Indian journal of medical research","volume":"135(6)","author":"M Biswal","year":"2012"},{"key":"ref33","first-page":"1932","article-title":"Lyssavirus detection and typing using pyrosequencing. J Clin Microbiol","volume":"49(5)","author":"P De Benedictis","year":"2011"},{"key":"ref34","first-page":"1410","article-title":"A reliable diagnosis of human rabies based on analysis of skin biopsy specimens. Clin Infect Dis","volume":"47(11)","author":"L Dacheux","year":"2008"},{"key":"ref35","first-page":"1921","article-title":"Development of a hemi-nested RT-PCR method for the specific determination of European Bat Lyssavirus 1. Comparison with other rabies diagnostic methods. Vaccine","volume":"22(15\u201316)","author":"E Picard-Meyer","year":"2004"},{"key":"ref36","first-page":"109","article-title":"Detection of rabies viral RNA by TaqMan real-time RT-PCR using non-neural specimens from dogs infected with rabies virus. J Virol Methods","volume":"184(1\u20132)","author":"S Wacharapluesadee","year":"2012"},{"key":"ref37","first-page":"3949","article-title":"Improved PCR methods for detection of African rabies and rabies-related lyssaviruses. J Clin Microbiol","volume":"48(11)","author":"J Coertse","year":"2010"},{"key":"ref38","first-page":"317","article-title":"Development of a TaqMan real-time RT-PCR assay for the detection of rabies virus. J Virol Methods","volume":"151(2)","author":"S Wacharapluesadee","year":"2008"},{"key":"ref39","first-page":"53","article-title":"Real-time PCR analysis of dog cerebrospinal fluid and saliva samples for ante-mortem diagnosis of rabies. Southeast Asian J Trop Med Public Health","volume":"38(1)","author":"W Saengseesom","year":"2007"},{"key":"ref40","first-page":"17","article-title":"Ante mortem diagnosis of human rabies using saliva samples: comparison of real time and conventional RT-PCR techniques. J Clin Virol","volume":"36(1)","author":"T Nagaraj","year":"2006"},{"key":"ref41","first-page":"25","article-title":"A rapid RT-PCR method to differentiate six established genotypes of rabies and rabies-related viruses using TaqMan technology. 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