{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T10:27:39Z","timestamp":1784543259614,"version":"3.55.0"},"reference-count":15,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2013,4,18]],"date-time":"2013-04-18T00:00:00Z","timestamp":1366243200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0061451","type":"journal-article","created":{"date-parts":[[2013,4,18]],"date-time":"2013-04-18T21:15:36Z","timestamp":1366319736000},"page":"e61451","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":46,"title":["The Development and Application of the Two Real-Time RT-PCR Assays to Detect the Pathogen of HFMD"],"prefix":"10.1371","volume":"8","author":[{"given":"Aili","family":"Cui","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changping","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojuan","family":"Tan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naiying","family":"Mao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiyu","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenbo","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2013,4,18]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"929","DOI":"10.1056\/NEJM199909233411301","article-title":"An epidemic of enterovirus 71 infection in Taiwan. Taiwan Enterovirus Epidemic Working Group","volume":"341","author":"M Ho","year":"1999","journal-title":"N Engl J Med"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"461","DOI":"10.3201\/eid0904.020395","article-title":"Molecular epidemiology of human enterovirus 71 strains and recent outbreaks in the Asia-Pacific region: comparative analysis of the VP1 and VP4 genes","volume":"9","author":"MJ Cardosa","year":"2003","journal-title":"Emerg Infect Dis"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"S52","DOI":"10.1086\/338819","article-title":"The 1998 enterovirus 71 outbreak in Taiwan: pathogenesis and management","volume":"34","author":"TY Lin","year":"2002","journal-title":"Clin Infect Dis"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"309","DOI":"10.4049\/jimmunol.84.3.309","article-title":"Typing of virus by combination of antiserum pools. Application to typing of enteroviruses","volume":"84","author":"KA Lim","year":"1960","journal-title":"J Immunol"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"3690","DOI":"10.1128\/JCM.39.10.3690-3692.2001","article-title":"Serotype-specific detection of coxsackievirus A16 in clinical specimens by reverse transcription-nested PCR","volume":"39","author":"JW Bendig","year":"2001","journal-title":"J Clin Microbiol"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/S1386-6532(00)00065-2","article-title":"Serotype-specific identification of enterovirus 71 by PCR","volume":"16","author":"BA Brown","year":"2000","journal-title":"J Clin Virol"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"2698","DOI":"10.1128\/JCM.00542-06","article-title":"Sensitive, seminested PCR amplification of VP1 sequences for direct identification of all enterovirus serotypes from original clinical specimens","volume":"44","author":"WA Nix","year":"2006","journal-title":"J Clin Microbiol"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"2823","DOI":"10.1128\/JCM.40.8.2823-2827.2002","article-title":"Direct detection of enterovirus 71 (EV71) in clinical specimens from a hand, foot, and mouth disease outbreak in Singapore by reverse transcription-PCR with universal enterovirus and EV71-specific primers","volume":"40","author":"S Singh","year":"2002","journal-title":"J Clin Microbiol"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/S0166-0934(01)00376-7","article-title":"Use of molecular assay in diagnosis of hand, foot and mouth disease caused by enterovirus 71 or coxsackievirus A 16","volume":"102","author":"KC Tsao","year":"2002","journal-title":"J Virol Methods"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"1292","DOI":"10.1093\/nar\/30.6.1292","article-title":"Real-time PCR in virology","volume":"30","author":"IM Mackay","year":"2002","journal-title":"Nucleic Acids Res"},{"key":"ref11","unstructured":"Melnick J (1996) Enteroviruses: polioviruses, coxsackieviruses, echoviruses, and newer enteroviruses. In: Fields virology, ed. Fields BN, Knipe DM, Howley PM, Eds. Philadelphia: Lippincott-Raven Publishers. 655\u2013712 p."},{"key":"ref12","doi-asserted-by":"crossref","first-page":"311","DOI":"10.3201\/eid1002.030759","article-title":"Real-time reverse transcription-polymerase chain reaction assay for SARS-associated coronavirus","volume":"10","author":"SL Emery","year":"2004","journal-title":"Emerg Infect Dis"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1128\/JCM.39.1.196-200.2001","article-title":"Simultaneous detection of influenza viruses A and B using real-time quantitative PCR","volume":"39","author":"LJ van Elden","year":"2001","journal-title":"J Clin Microbiol"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"1941","DOI":"10.1128\/JVI.73.3.1941-1948.1999","article-title":"Molecular evolution of the human enteroviruses: correlation of serotype with VP1 sequence and application to picornavirus classification","volume":"73","author":"MS Oberste","year":"1999","journal-title":"J Virol"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1016\/j.jcv.2009.02.002","article-title":"An outbreak of hand, foot, and mouth disease associated with subgenotype C4 of human enterovirus 71 in Shandong, China","volume":"44","author":"Y Zhang","year":"2009","journal-title":"J Clin Virol"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0061451","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,1]],"date-time":"2023-07-01T05:33:04Z","timestamp":1688189584000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0061451"}},"subtitle":[],"editor":[{"given":"Ravi","family":"Jhaveri","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2013,4,18]]},"references-count":15,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2013,4,18]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0061451","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,4,18]]}}}