{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T16:58:24Z","timestamp":1790355504555,"version":"4.1.0"},"reference-count":50,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2013,4,22]],"date-time":"2013-04-22T00:00:00Z","timestamp":1366588800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0061833","type":"journal-article","created":{"date-parts":[[2013,4,22]],"date-time":"2013-04-22T23:05:08Z","timestamp":1366671908000},"page":"e61833","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":38,"title":["Preferential Biological Processes in the Human Limbus by Differential Gene Profiling"],"prefix":"10.1371","volume":"8","author":[{"given":"Martin N.","family":"Nakatsu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lily","family":"Vartanyan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel M.","family":"Vu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Madelena Y.","family":"Ng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinmin","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophie X.","family":"Deng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2013,4,22]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"560","DOI":"10.1038\/229560a0","article-title":"Role of the pericorneal papillary structure in renewal of corneal epithelium","volume":"229","author":"M Davanger","year":"1971","journal-title":"Nature"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1016\/j.exer.2003.09.008","article-title":"Corneal epithelial stem cells at the limbus: looking at some old problems from a new angle","volume":"78","author":"RM Lavker","year":"2004","journal-title":"Exp Eye Res"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1083\/jcb.103.1.49","article-title":"Differentiation-related expression of a major 64K corneal keratin in vivo and in culture suggests limbal location of corneal epithelial stem cells","volume":"103","author":"A Schermer","year":"1986","journal-title":"J Cell Biol"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/0092-8674(89)90958-6","article-title":"Existence of slow-cycling limbal epithelial basal cells that can be preferentially stimulated to proliferate: implications on epithelial stem cells","volume":"57","author":"G Cotsarelis","year":"1989","journal-title":"Cell"},{"key":"ref5","first-page":"1450","article-title":"Comparison of central and peripheral human corneal epithelium in tissue culture","volume":"28","author":"B Ebato","year":"1987","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"769","DOI":"10.1083\/jcb.145.4.769","article-title":"Location and clonal analysis of stem cells and their differentiated progeny in the human ocular surface","volume":"145","author":"G Pellegrini","year":"1999","journal-title":"J Cell Biol"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"892","DOI":"10.1167\/iovs.05-1006","article-title":"The side population cells in the rabbit limbus sensitively increased in response to the central cornea wounding","volume":"47","author":"KS Park","year":"2006","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"2867","DOI":"10.1242\/jcs.111.19.2867","article-title":"Strategies of epithelial repair: modulation of stem cell and transit amplifying cell proliferation","volume":"19)","author":"MS Lehrer","year":"1998","journal-title":"J Cell Sci 111 (Pt"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"1715","DOI":"10.1242\/jcs.02279","article-title":"Ocular surface epithelia contain ABCG2-dependent side population cells exhibiting features associated with stem cells","volume":"118","author":"MT Budak","year":"2005","journal-title":"J Cell Sci"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1634\/stemcells.22-3-355","article-title":"Characterization of putative stem cell phenotype in human limbal epithelia","volume":"22","author":"Z Chen","year":"2004","journal-title":"Stem Cells"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"3156","DOI":"10.1073\/pnas.061032098","article-title":"p63 identifies keratinocyte stem cells","volume":"98","author":"G Pellegrini","year":"2001","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"9523","DOI":"10.1073\/pnas.0503437102","article-title":"Isoforms of DeltaNp63 and the migration of ocular limbal cells in human corneal regeneration","volume":"102","author":"E Di Iorio","year":"2005","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1016\/j.exer.2005.07.001","article-title":"Expression and tissue distribution of p63 isoforms in human ocular surface epithelia","volume":"82","author":"S Kawasaki","year":"2006","journal-title":"Exp Eye Res"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1634\/stemcells.2006-0167","article-title":"N-Cadherin is expressed by putative stem\/progenitor cells and melanocytes in the human limbal epithelial stem cell niche","volume":"25","author":"R Hayashi","year":"2007","journal-title":"Stem Cells"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"1037","DOI":"10.1083\/jcb.200703003","article-title":"C\/EBPdelta regulates cell cycle and self-renewal of human limbal stem cells","volume":"177","author":"V Barbaro","year":"2007","journal-title":"J Cell Biol"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1167\/iovs.06-0346","article-title":"The phenotype of limbal epithelial stem cells","volume":"48","author":"EC Figueira","year":"2007","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1016\/S0065-1281(11)80106-6","article-title":"Cytokeratin and vimentin heterogeneity in human cornea","volume":"93","author":"M Kasper","year":"1992","journal-title":"Acta Histochem"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/0012-1606(92)90209-Y","article-title":"Alpha-enolase is restricted to basal cells of stratified squamous epithelium","volume":"151","author":"JD Zieske","year":"1992","journal-title":"Dev Biol"},{"key":"ref19","first-page":"2031","article-title":"Preferential gene expression in the limbus of the vervet monkey","volume":"14","author":"Z Ding","year":"2008","journal-title":"Mol Vis"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"1142","DOI":"10.1016\/j.biocel.2010.03.022","article-title":"Molecular signatures and biological pathway profiles of human corneal epithelial progenitor cells","volume":"42","author":"F Bian","year":"2010","journal-title":"Int J Biochem Cell Biol"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"526","DOI":"10.1186\/1471-2164-11-526","article-title":"Comparative transcriptional profiling of the limbal epithelial crypt demonstrates its putative stem cell niche characteristics","volume":"11","author":"BB Kulkarni","year":"2010","journal-title":"BMC Genomics"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"1252","DOI":"10.1167\/iovs.10-5242","article-title":"Differentially expressed genes associated with human limbal epithelial phenotypes: new molecules that potentially facilitate selection of stem cell-enriched populations","volume":"52","author":"L Takacs","year":"2010","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"2050","DOI":"10.1167\/iovs.06-0998","article-title":"Comparative analysis of human conjunctival and corneal epithelial gene expression with oligonucleotide microarrays","volume":"48","author":"HC Turner","year":"2007","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"5630","DOI":"10.1167\/iovs.09-3791","article-title":"Differential gene expression in the pig limbal side population: implications for stem cell cycling, replication, and survival","volume":"50","author":"MA Akinci","year":"2009","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"19600","DOI":"10.1074\/jbc.M600777200","article-title":"Transcriptional profiling of enriched populations of stem cells versus transient amplifying cells. A comparison of limbal and corneal epithelial basal cells","volume":"281","author":"M Zhou","year":"2006","journal-title":"J Biol Chem"},{"key":"ref26","first-page":"1652","article-title":"Keratin 13 is a more specific marker of conjunctival epithelium than keratin 19","volume":"17","author":"A Ramirez-Miranda","year":"2011","journal-title":"Mol Vis"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1073\/pnas.98.1.31","article-title":"Model-based analysis of oligonucleotide arrays: expression index computation and outlier detection","volume":"98","author":"C Li","year":"2001","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"310","DOI":"10.1016\/j.ydbio.2008.02.030","article-title":"The canonical Wnt signaling antagonist DKK2 is an essential effector of PITX2 function during normal eye development","volume":"317","author":"PJ Gage","year":"2008","journal-title":"Dev Biol"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1016\/S0092-8674(02)01084-X","article-title":"Identification of a Wnt\/Dvl\/beta-Catenin \u2013&gt;Pitx2 pathway mediating cell-type-specific proliferation during development","volume":"111","author":"C Kioussi","year":"2002","journal-title":"Cell"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1016\/j.exer.2007.08.020","article-title":"Characterization of extracellular matrix components in the limbal epithelial stem cell compartment","volume":"85","author":"U Schlotzer-Schrehardt","year":"2007","journal-title":"Exp Eye Res"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"4734","DOI":"10.1167\/iovs.10-6486","article-title":"Wnt\/\u03b2-catenin Signaling Regulates Proliferation of Human Cornea Epithelial Stem\/Progenitor Cells","volume":"52","author":"MN Nakatsu","year":"2011","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"897","DOI":"10.1515\/BC.2008.108","article-title":"The WNT receptor FZD7 contributes to self-renewal signaling of human embryonic stem cells","volume":"389","author":"K Melchior","year":"2008","journal-title":"Biol Chem"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.stem.2012.09.015","article-title":"Fibronectin regulates Wnt7a signaling and satellite cell expansion","volume":"12","author":"CF Bentzinger","year":"2013","journal-title":"Cell Stem Cell"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"828","DOI":"10.1167\/iovs.03-0309","article-title":"Characterization and prevalence of PITX2 microdeletions and mutations in Axenfeld-Rieger malformations","volume":"45","author":"MA Lines","year":"2004","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"2149","DOI":"10.1242\/dev.02381","article-title":"Dkk2 plays an essential role in the corneal fate of the ocular surface epithelium","volume":"133","author":"M Mukhopadhyay","year":"2006","journal-title":"Development"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"4355","DOI":"10.1074\/jbc.M112.409102","article-title":"Wnt\/beta-Catenin Pathway Is Regulated by PITX2 Homeodomain Protein and Thus Contributes to the Proliferation of Human Ovarian Adenocarcinoma Cell, SKOV-3","volume":"288","author":"M Basu","year":"2013","journal-title":"J Biol Chem"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1002\/(SICI)1097-0177(200006)218:2<235::AID-DVDY2>3.0.CO;2-G","article-title":"The tenascin family of ECM glycoproteins: structure, function, and regulation during embryonic development and tissue remodeling","volume":"218","author":"FS Jones","year":"2000","journal-title":"Dev Dyn"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1159\/000112848","article-title":"Tenascin C in stem cell niches: redundant, permissive or instructive?","volume":"188","author":"A von Holst","year":"2008","journal-title":"Cells Tissues Organs"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/S0014-5793(01)02520-0","article-title":"Identification of human endomucin-1 and -2 as membrane-bound O-sialoglycoproteins with anti-adhesive activity","volume":"499","author":"M Kinoshita","year":"2001","journal-title":"FEBS Lett"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"844","DOI":"10.1002\/dvdy.22216","article-title":"Fibromodulin regulates collagen fibrillogenesis during peripheral corneal development","volume":"239","author":"S Chen","year":"2010","journal-title":"Dev Dyn"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1042\/bj3020527","article-title":"Interaction of the small interstitial proteoglycans biglycan, decorin and fibromodulin with transforming growth factor beta","volume":"2)","author":"A Hildebrand","year":"1994","journal-title":"Biochem J 302 (Pt"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1016\/j.bbrc.2008.04.095","article-title":"Fibromodulin gene is expressed in human epidermal keratinocytes in culture and in human epidermis in vivo","volume":"371","author":"C Velez-Delvalle","year":"2008","journal-title":"Biochem Biophys Res Commun"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1038\/cr.2008.323","article-title":"Roles of TGF-beta family signaling in stem cell renewal and differentiation","volume":"19","author":"T Watabe","year":"2009","journal-title":"Cell Res"},{"key":"ref44","first-page":"1922","article-title":"Transforming growth factor-beta receptor expression in human cornea","volume":"38","author":"NC Joyce","year":"1997","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"ref45","first-page":"23","article-title":"Immunolocalization of TGF-beta 1, TGF-beta 2, and TGF-beta 3 in the anterior segment of the human eye","volume":"34","author":"LR Pasquale","year":"1993","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"ref46","first-page":"3289","article-title":"Immunohistochemical localization of transforming growth factor-beta 1, -beta 2, and -beta 3 latency-associated peptide in human cornea","volume":"35","author":"K Nishida","year":"1994","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"ref47","first-page":"296","article-title":"Bone morphogenetic proteins and growth and differentiation factors in the human cornea","volume":"40","author":"L You","year":"1999","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"1117","DOI":"10.1038\/ng1430","article-title":"BMP signaling inhibits intestinal stem cell self-renewal through suppression of Wnt-beta-catenin signaling","volume":"36","author":"XC He","year":"2004","journal-title":"Nat Genet"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"10063","DOI":"10.1073\/pnas.0703004104","article-title":"Loss of a quiescent niche but not follicle stem cells in the absence of bone morphogenetic protein signaling","volume":"104","author":"K Kobielak","year":"2007","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref50","doi-asserted-by":"crossref","first-page":"17156","DOI":"10.1074\/jbc.M513812200","article-title":"A dishevelled-1\/Smad1 interaction couples WNT and bone morphogenetic protein signaling pathways in uncommitted bone marrow stromal cells","volume":"281","author":"Z Liu","year":"2006","journal-title":"J Biol Chem"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0061833","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,14]],"date-time":"2022-02-14T11:44:51Z","timestamp":1644839091000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0061833"}},"subtitle":[],"editor":[{"given":"Shukti","family":"Chakravarti","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2013,4,22]]},"references-count":50,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2013,4,22]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0061833","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,4,22]]}}}