{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T19:55:51Z","timestamp":1789847751733,"version":"4.0.1"},"reference-count":40,"publisher":"Public Library of Science (PLoS)","issue":"7","license":[{"start":{"date-parts":[[2013,7,5]],"date-time":"2013-07-05T00:00:00Z","timestamp":1372982400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0062473","type":"journal-article","created":{"date-parts":[[2013,4,23]],"date-time":"2013-04-23T22:25:19Z","timestamp":1366755919000},"page":"e62473","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":30,"title":["Detection of DNA from Leishmania (Viannia): Accuracy of Polymerase Chain Reaction for the Diagnosis of Cutaneous Leishmaniasis"],"prefix":"10.1371","volume":"8","author":[{"given":"Herintha Coeto","family":"Neitzke-Abreu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mateus Sabaini","family":"Venazzi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcos Vinicius Zandonadi","family":"Bernal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K\u00e1rin Rosi","family":"Reinhold-Castro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernanda","family":"Vagetti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camila Alves","family":"Mota","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naielly Rodrigues","family":"Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra Mara Alessi","family":"Aristides","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tha\u00eds Gomes Verzignassi","family":"Silveira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Valdrinez Campana","family":"Lonardoni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2013,7,5]]},"reference":[{"key":"ref1","unstructured":"World Health Organization (2010) Control of the leishmaniases. Available: <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/goo.gl\/1WxXs\" xlink:type=\"simple\">http:\/\/goo.gl\/1WxXs<\/ext-link>. Accessed 10 November 2012."},{"key":"ref2","first-page":"71","article-title":"Cutaneous Leishmaniosis","volume":"36","author":"B Gontijo","year":"2003","journal-title":"Rev Soc Bras Med Trop"},{"key":"ref3","unstructured":"Brasil Ministry of Health (2007) Manual for the surveillance of American cutaneous leishmaniasis. Available: <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/goo.gl\/mPcbz\" xlink:type=\"simple\">http:\/\/goo.gl\/mPcbz<\/ext-link>. Accessed 10 November 2012."},{"key":"ref4","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1016\/S0738-081X(99)00047-4","article-title":"Cutaneous leishmaniasis: clinical features and diagnosis","volume":"17","author":"SM Salman","year":"1999","journal-title":"Clin Dermatol"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1002\/path.1711590406","article-title":"Histological analysis and the pathogenesis of mucocutaneous leishmaniasis","volume":"159","author":"DS Ridley","year":"1989","journal-title":"J Pathol"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1590\/S0036-46651996000300003","article-title":"Cross-reactivity of antibodies in human infections by kinetoplastid protozoa <italic>Trypanosoma cruzi<\/italic>, <italic>Leishmania chagasi<\/italic> and <italic>Leishmania (Viannia) braziliensis<\/italic>","volume":"38","author":"AC Vexenat","year":"1996","journal-title":"Rev Inst Med Trop S\u00e3o Paulo"},{"key":"ref7","unstructured":"Silva CEA (2008) Evaluation of polymerase chain reaction for diagnosis of cutaneous leishmaniasis in patients from endemic areas. Masters dissertation of the Universidade Estadual de Maring\u00e1, Maring\u00e1, Brazil."},{"key":"ref8","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1590\/S0074-02762006000400014","article-title":"Polymerase chain reaction with lesion scrapping for the dianosis of human American tegumentary leishmaniasis","volume":"101","author":"EAS Venazzi","year":"2006","journal-title":"Mem Inst Oswaldo Cruz"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1016\/j.exppara.2006.10.002","article-title":"Detection of <italic>Leishmania (Viannia)<\/italic> DNA in blood from patients with American cutaneous leishmaniasis","volume":"115","author":"EAS Venazzi","year":"2007","journal-title":"Exp Parasitol"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.actatropica.2004.12.003","article-title":"Polymerase chain reaction (PCR) is highly sensitive for diagnosis of mucosal leishmaniasis","volume":"94","author":"JGS Oliveira","year":"2005","journal-title":"Acta Trop"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"999","DOI":"10.4269\/ajtmh.2005.73.999","article-title":"Rapid identification of <italic>Leishmania<\/italic> complexes by a real-time PCR assay","volume":"73","author":"G Wortmann","year":"2005","journal-title":"Am J Trop Med Hyg"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.diagmicrobio.2005.12.001","article-title":"Development of a direct species-specific PCR assay for differential diagnosis of <italic>Leishmania tropica<\/italic>","volume":"55","author":"M Jirk\u00f9","year":"2006","journal-title":"Diagn Microbiol Infect Dis"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"323","DOI":"10.3185\/pathexo3201","article-title":"Epidemiology of <italic>Leishmania (L.) infantum<\/italic>, <italic>L. major<\/italic> and <italic>L. killicki<\/italic> in Tunisia: results and analysis of the identification of 226 human and canine isolates","volume":"101","author":"K Aoun","year":"2008","journal-title":"Bull Soc Pathol Exot"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1590\/S0036-46652002000500004","article-title":"Assessment of PCR in the detection of <italic>Leishmania<\/italic> spp in experimentally infected individual phlebotomine sandflies (Diptera: Psychodidae: Phlebotominae)","volume":"44","author":"EM Michalsky","year":"2002","journal-title":"Rev Inst Med Trop S\u00e3o Paulo"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1016\/j.exppara.2008.08.005","article-title":"<italic>Leishmania (Viannia) braziliensis<\/italic>: New primers for identification using polymerase chain reaction","volume":"120","author":"VM Marcussi","year":"2008","journal-title":"Exp Parasitol"},{"key":"ref16","first-page":"17","article-title":"Research of natural infection of phlebotomines for <italic>Leishmania<\/italic>, in the State of Paran\u00e1","volume":"41","author":"HC Neitzke","year":"2008","journal-title":"Rev Soc Bras Med Trop"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1590\/S0074-02762010000100018","article-title":"Evaluation of polymerase chain reaction in the routine diagnosis for tegumentary leishmaniasis in a referral centre","volume":"105","author":"A Fagundes","year":"2010","journal-title":"Mem Inst Oswaldo Cruz"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1089\/vbz.2009.0218","article-title":"Natural infection of <italic>Nyssomyia neivai<\/italic> by <italic>Leishmania (Viannia)<\/italic> spp. in the State of Paran\u00e1, Southern Brazil, detected by Multiplex Polymerase Chain Reaction","volume":"11","author":"DM Oliveira","year":"2011","journal-title":"Vector-Borne Zoonotic Dis"},{"key":"ref19","first-page":"413","article-title":"Observations on laboratory diagnosis and cutaneous leishmaniasis epidemiology in the State of Paran\u00e1 State, South of Brazil","volume":"32","author":"TGV Silveira","year":"1999","journal-title":"Rev Soc Bras Med Trop"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1002\/jcla.20140","article-title":"Chemiluminescent determination of leukocyte alkaline phosphatase: an advantageous alternative to the cytochemical assay","volume":"21","author":"MPP Kanegae","year":"2007","journal-title":"J Clin Lab Anal"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"348","DOI":"10.4269\/ajtmh.1993.49.348","article-title":"Diagnosis of <italic>Leishmania<\/italic> using the polymerase chain reaction: a simplified procedure for field work","volume":"49","author":"M Lopez","year":"1993","journal-title":"Am J Trop Med Hyg"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1038\/324163a0","article-title":"Analysis of enzymatically amplified beta-globin and HLA-DQ alpha DNA with allele-specific oligonucleotide probes","volume":"324","author":"RK Saiki","year":"1986","journal-title":"Nature"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/S0035-9203(02)90070-2","article-title":"Validation of the polymerase chain reaction for the diagnosis of human cutaneous leishmaniasis in north-west Colombia","volume":"96","author":"DM Isaza","year":"2002","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"102","DOI":"10.4269\/ajtmh.1998.58.102","article-title":"Simplified polymerase chain reaction detection of new world <italic>Leishmania<\/italic> in clinical specimens of cutaneous leishmaniasis","volume":"58","author":"A Belli","year":"1998","journal-title":"Am J Trop Med Hyg"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"1989","DOI":"10.1128\/JCM.36.7.1989-1995.1998","article-title":"Single-step multiplex PCR for characterization of New World <italic>Leishmania<\/italic> complexes","volume":"36","author":"E Harris","year":"1998","journal-title":"J Clin Microbiol"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"181","DOI":"10.2307\/3285616","article-title":"PCR detection and identification of <italic>Leishmania<\/italic> parasite in clinical specimens in Ecuador: a comparison with classical diagnostic methods","volume":"85","author":"H Aviles","year":"1999","journal-title":"J Parasitol"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1590\/S0100-879X2002000400002","article-title":"Comparison of the specificity of PCR and the histopathological detection of <italic>Leishmania<\/italic> for the diagnosis of American cutaneous leishmaniasis","volume":"35","author":"ACR Medeiros","year":"2002","journal-title":"Braz J Med Biol Res"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1128\/CDLI.1.4.385-389.1994","article-title":"Presence of <italic>Leishmania braziliensis<\/italic> in blood samples from cured patients or at different stages of immunotherapy","volume":"1","author":"P Guevara","year":"1994","journal-title":"Clin Diag Lab Immunol"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"1145","DOI":"10.1128\/JCM.33.5.1145-1149.1995","article-title":"PCR and in vitro cultivation for detection of <italic>Leishmania<\/italic> spp. in diagnostic samples from humans and dogs","volume":"33","author":"A Mathis","year":"1995","journal-title":"J Clin Microbiol"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"748","DOI":"10.1128\/JCM.38.2.748-751.2000","article-title":"Use of PCR to detect <italic>Leishmania (Viannia)<\/italic> spp. in dog blood and bone marrow","volume":"38","author":"R Reithinger","year":"2000","journal-title":"J Clin Microbiol"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"1112","DOI":"10.1016\/j.trstmh.2006.02.014","article-title":"Haematogenous dissemination of <italic>Leishmania (Viannia) braziliensis<\/italic> in human American tegumentary leishmaniasis","volume":"100","author":"PO Camera","year":"2006","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1016\/S1413-8670(11)70176-3","article-title":"Comparison of different primes for PCR-based diagnosis of cutaneous leishmaniasis","volume":"15","author":"DM Oliveira","year":"2011","journal-title":"Braz J Infect Dis"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1590\/S0074-02761990000400014","article-title":"An autochthonous case of cutaneous leishmaniasis caused by <italic>Leishmania (Leishmania) amazonensis<\/italic> Lainson &amp; Shaw, 1972 from the north of Paran\u00e1 State, Brazil","volume":"85","author":"TGV Silveira","year":"1990","journal-title":"Mem Inst Oswaldo Cruz"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"902","DOI":"10.4269\/ajtmh.2001.65.902","article-title":"Simple form clinical sample preservation and <italic>Leishmania<\/italic> DNA extraction from human lesions for diagnosis of american cutaneous leishmaniasis via polymerase chain reaction","volume":"65","author":"MJ Marques","year":"2001","journal-title":"Am J Trop Med Hyg"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"1341","DOI":"10.1016\/0140-6736(93)90845-8","article-title":"<italic>Leishmania braziliensis<\/italic> in blood 30 years after cure","volume":"341","author":"P Guevara","year":"1993","journal-title":"Lancet"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1590\/S0074-02761997000300006","article-title":"Persistent Infections by <italic>Leishmania (Viannia) braziliensis<\/italic>","volume":"92","author":"JL Ram\u00edrez","year":"1997","journal-title":"Mem Inst Oswaldo Cruz"},{"key":"ref37","first-page":"205","article-title":"Serological investigation of subclinical cutaneous leishmaniasis cases following an outbreak in an endemic area","volume":"41","author":"SMA Arraes","year":"2008","journal-title":"Rev Soc Bras Med Trop"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"1018","DOI":"10.1086\/382135","article-title":"Persistence of <italic>Leishmania<\/italic> parasites in scars after clinical cure of American Cutaneous Leishmaniasis: Is There a Sterile Cure?","volume":"189","author":"MG Mendon\u00e7a","year":"2004","journal-title":"J Infect Dis"},{"key":"ref39","first-page":"28","article-title":"PCR detection of specific <italic>Leishmania<\/italic>-DNA in patients with periodontal disease","volume":"94","author":"G Premoli-de-Percoco","year":"2002","journal-title":"Pathologica"},{"key":"ref40","unstructured":"Manson-Bahr PE (1987) Diagnosis. In: Peters W, Kilich-Kendrick R, editors. The Leishmaniasis. London: Academic Press. 703\u2013728."}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0062473","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,7,25]],"date-time":"2020-07-25T20:07:22Z","timestamp":1595707642000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0062473"}},"subtitle":[],"editor":[{"given":"Dan","family":"Zilberstein","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2013,7,5]]},"references-count":40,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2013,7,5]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0062473","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,7,5]]}}}