{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,28]],"date-time":"2026-02-28T19:27:27Z","timestamp":1772306847898,"version":"3.50.1"},"reference-count":35,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2013,5,13]],"date-time":"2013-05-13T00:00:00Z","timestamp":1368403200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0063336","type":"journal-article","created":{"date-parts":[[2013,5,13]],"date-time":"2013-05-13T21:08:41Z","timestamp":1368479321000},"page":"e63336","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":11,"title":["Malonate Inhibits Virulence Gene Expression in Vibrio cholerae"],"prefix":"10.1371","volume":"8","author":[{"given":"Yusuke","family":"Minato","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara R.","family":"Fassio","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia C.","family":"H\u00e4se","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2013,5,13]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"3042","DOI":"10.1128\/IAI.58.9.3042-3049.1990","article-title":"<italic>Vibrio cholerae<\/italic> O395 <italic>tcpA<\/italic> pilin gene sequence and comparison of predicted protein structural features to those of type 4 pilins","volume":"58","author":"CE Shaw","year":"1990","journal-title":"Infect Immun"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"1487","DOI":"10.1084\/jem.168.4.1487","article-title":"Toxin, toxin-coregulated pili, and the <italic>toxR<\/italic> regulon are essential for <italic>Vibrio cholerae<\/italic> pathogenesis in humans","volume":"168","author":"DA Herrington","year":"1988","journal-title":"J Exp Med"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"2833","DOI":"10.1073\/pnas.84.9.2833","article-title":"Use of <italic>phoA<\/italic> gene fusions to identify a pilus colonization factor coordinately regulated with cholera toxin","volume":"84","author":"RK Taylor","year":"1987","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"1771","DOI":"10.1016\/j.biocel.2007.07.005","article-title":"<italic>Vibrio cholerae<\/italic>: cholera toxin","volume":"39","author":"D Vanden Broeck","year":"2007","journal-title":"Int J Biochem Cell Biol"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"5403","DOI":"10.1073\/pnas.88.12.5403","article-title":"Regulatory cascade controls virulence in <italic>Vibrio cholerae<\/italic>","volume":"88","author":"VJ DiRita","year":"1991","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"5542","DOI":"10.1128\/IAI.01094-07","article-title":"Regulatory networks controlling <italic>Vibrio cholerae<\/italic> virulence gene expression","volume":"75","author":"JS Matson","year":"2007","journal-title":"Infect Immun"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"730","DOI":"10.1073\/pnas.95.2.730","article-title":"TcpP protein is a positive regulator of virulence gene expression in <italic>Vibrio cholerae<\/italic>","volume":"95","author":"CC H\u00e4se","year":"1998","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1046\/j.1365-2958.1999.01215.x","article-title":"A new level in the <italic>Vibrio cholerae<\/italic> ToxR virulence cascade: AphA is required for transcriptional activation of the <italic>tcpPH<\/italic> operon","volume":"31","author":"K Skorupski","year":"1999","journal-title":"Mol Microbiol"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"4250","DOI":"10.1128\/JB.181.14.4250-4256.1999","article-title":"A <italic>Vibrio cholerae<\/italic> LysR homolog, AphB, cooperates with AphA at the <italic>tcpPH<\/italic> promoter to activate expression of the ToxR virulence cascade","volume":"181","author":"G Kovacikova","year":"1999","journal-title":"J Bacteriol"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1046\/j.1365-2958.1997.4611824.x","article-title":"Enzymic and genetic basis for bacterial growth on malonate","volume":"25","author":"P Dimroth","year":"1997","journal-title":"Mol Microbiol"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"3183","DOI":"10.1073\/pnas.96.6.3183","article-title":"Effects of changes in membrane sodium flux on virulence gene expression in <italic>Vibrio cholerae<\/italic>","volume":"96","author":"CC H\u00e4se","year":"1999","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref12","doi-asserted-by":"crossref","unstructured":"Minato Y, Fassio SR, Wolfe AJ, H\u00e4se CC (2013) Central metabolism controls transcription of a virulence gene regulator in <italic>Vibrio cholerae<\/italic>. Microbiology (in press).","DOI":"10.1099\/mic.0.064865-0"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"2314","DOI":"10.1128\/JCM.25.12.2314-2316.1987","article-title":"Large production of cholera toxin by <italic>Vibrio cholerae<\/italic> O1 in yeast extract peptone water","volume":"25","author":"M Iwanaga","year":"1987","journal-title":"J Clin Microbiol"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"2822","DOI":"10.1128\/IAI.56.11.2822-2829.1988","article-title":"Characterization of the <italic>Vibrio cholerae<\/italic> ToxR regulon - Identification of novel genes involved in intestinal colonization","volume":"56","author":"KM Peterson","year":"1988","journal-title":"Infect Immun"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"5850","DOI":"10.1128\/JB.05222-11","article-title":"TolC affects virulence gene expression in <italic>Vibrio cholerae<\/italic>","volume":"193","author":"Y Minato","year":"2011","journal-title":"J Bacteriol"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1016\/0076-6879(94)35167-8","article-title":"Regulation of cholera toxin by temperature, pH, and osmolarity","volume":"235","author":"CL Gardel","year":"1994","journal-title":"Methods Enzymol"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1128\/MMBR.69.1.12-50.2005","article-title":"The acetate switch","volume":"69","author":"AJ Wolfe","year":"2005","journal-title":"Microbiol Mol Biol Rev"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1016\/j.mib.2010.01.002","article-title":"Physiologically relevant small phosphodonors link metabolism to signal transduction","volume":"13","author":"AJ Wolfe","year":"2010","journal-title":"Curr Opin Microbiol"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1099\/00221287-102-2-327","article-title":"The enzymic interconversion of acetate and acetyl-coenzyme A in <italic>Escherichia coli<\/italic>","volume":"102","author":"TD Brown","year":"1977","journal-title":"J Gen Microbiol"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1016\/S0021-9258(18)71161-7","article-title":"Enzymatic phosphorylation of acetate","volume":"211","author":"IA Rose","year":"1954","journal-title":"J Biol Chem"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"1133","DOI":"10.1111\/j.1365-2958.1995.mmi_17061133.x","article-title":"Single amino acid substitutions in the N-terminus of <italic>Vibrio cholerae<\/italic> TcpA affect colonization, autoagglutination, and serum resistance","volume":"17","author":"SL Chiang","year":"1995","journal-title":"Mol Microbiol"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1128\/JCM.22.3.405-408.1985","article-title":"New medium for the production of cholera toxin by <italic>Vibrio cholerae<\/italic> O1 biotype El Tor","volume":"22","author":"M Iwanaga","year":"1985","journal-title":"J Clin Microbiol"},{"key":"ref23","unstructured":"Bergey DH, Holt JGE, Krieg NR, Sneath PHA, Mair NS, et al. (1984) Bergey\u2019s manual of systematic bacteriology: Williams and Wilkins."},{"key":"ref24","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1159\/000219374","article-title":"Correlations between carbon metabolism and virulence in bacteria","volume":"16","author":"S Poncet","year":"2009","journal-title":"Contrib Microbiol"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1128\/MMBR.00005-09","article-title":"At the crossroads of bacterial metabolism and virulence factor synthesis in <italic>Staphylococci<\/italic>","volume":"73","author":"GA Somerville","year":"2009","journal-title":"Microbiol Mol Biol Rev"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1038\/nrmicro2351","article-title":"Carbon metabolism of intracellular bacterial pathogens and possible links to virulence","volume":"8","author":"W Eisenreich","year":"2010","journal-title":"Nat Rev Microbiol"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1016\/j.cell.2012.01.053","article-title":"Coordinated regulation of accessory genetic elements produces cyclic di-Nucleotides for <italic>V. cholerae<\/italic> virulence","volume":"149","author":"BW Davies","year":"2012","journal-title":"Cell"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"3377","DOI":"10.1128\/JB.06379-11","article-title":"The Entner-Doudoroff pathway is obligatory for gluconate utilization and contributes to the pathogenicity of <italic>Vibrio cholerae<\/italic>","volume":"194","author":"T Patra","year":"2012","journal-title":"J Bacteriol"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"39742","DOI":"10.1074\/jbc.M112.394932","article-title":"Activation of cholera toxin production by anaerobic respiration of trimethylamine N-oxide in <italic>Vibrio cholerae<\/italic>","volume":"287","author":"KM Lee","year":"2012","journal-title":"J Biol Chem"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"e00236","DOI":"10.1128\/mBio.00236-12","article-title":"MetR-regulated <italic>Vibrio cholerae<\/italic> metabolism is required for virulence","volume":"3","author":"RW Bogard","year":"2012","journal-title":"MBio"},{"key":"ref31","first-page":"443","article-title":"Malonate metabolism: biochemistry, molecular biology, physiology, and industrial application","volume":"35","author":"YS Kim","year":"2002","journal-title":"J Biochem Mol Biol"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"530","DOI":"10.1111\/j.1432-1033.1997.00530.x","article-title":"Sequence of a gene cluster from <italic>Klebsiella pneumoniae<\/italic> encoding malonate decarboxylase and expression of the enzyme in <italic>Escherichia coli<\/italic>","volume":"246","author":"S Hoenke","year":"1997","journal-title":"Eur J Biochem"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1128\/IAI.00663-10","article-title":"A <italic>fadD<\/italic> mutant of <italic>Vibrio cholerae<\/italic> is impaired in the production of virulence factors and membrane localization of the virulence regulatory protein TcpP","volume":"79","author":"S Ray","year":"2011","journal-title":"Infect Immun"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"3781","DOI":"10.1021\/bi011873o","article-title":"Purification and characterization of the recombinant Na<sup>+<\/sup> translocating NADH:quinone oxidoreductase from <italic>Vibrio cholerae<\/italic>","volume":"41","author":"B Barquera","year":"2002","journal-title":"Biochemistry"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1093\/infdis\/138.2.143","article-title":"Isolation and characterization of protease-deficient mutants of <italic>Vibrio cholerae<\/italic>","volume":"138","author":"DR Schneider","year":"1978","journal-title":"J Infect Dis"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0063336","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,16]],"date-time":"2022-02-16T23:03:49Z","timestamp":1645052629000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0063336"}},"subtitle":[],"editor":[{"given":"Vasu D.","family":"Appanna","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2013,5,13]]},"references-count":35,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2013,5,13]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0063336","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,5,13]]}}}