{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T03:38:42Z","timestamp":1768880322422,"version":"3.49.0"},"reference-count":42,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2013,5,21]],"date-time":"2013-05-21T00:00:00Z","timestamp":1369094400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0063394","type":"journal-article","created":{"date-parts":[[2013,5,21]],"date-time":"2013-05-21T21:10:26Z","timestamp":1369170626000},"page":"e63394","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":19,"title":["Quorum Sensing Negatively Regulates Multinucleate Cell Formation during Intracellular Growth of Burkholderia pseudomallei in Macrophage-Like Cells"],"prefix":"10.1371","volume":"8","author":[{"given":"Rachel E.","family":"Horton","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gary D.","family":"Grant","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ben","family":"Matthews","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Batzloff","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suzzanne J.","family":"Owen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephanie","family":"Kyan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cameron P.","family":"Flegg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amanda M.","family":"Clark","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Glen C.","family":"Ulett","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nigel","family":"Morrison","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ian R.","family":"Peak","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ifor R.","family":"Beacham","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2013,5,21]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"e900","DOI":"10.1371\/journal.pntd.0000900","article-title":"The epidemiology and clinical spectrum of melioidosis: 540 cases from the 20 year Darwin prospective study","volume":"4","author":"BJ Currie","year":"2010","journal-title":"PLoS Negl Trop Dis"},{"key":"ref2","first-page":"272","article-title":"Melioidosis in northern Australia, 2001\u201302","volume":"27","author":"AC Cheng","year":"2003","journal-title":"Commun Dis Intell"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"1715","DOI":"10.1016\/S0140-6736(03)13374-0","article-title":"Melioidosis","volume":"361","author":"NJ White","year":"2003","journal-title":"Lancet"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/S0001-706X(99)00059-5","article-title":"Melioidosis as an emerging global problem","volume":"74","author":"DA Dance","year":"2000","journal-title":"Acta Trop"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"S1","DOI":"10.1016\/S0035-9203(08)70002-6","article-title":"The global distribution of <italic>Burkholderia pseudomallei<\/italic> and melioidosis: an update","volume":"102","author":"BJ Currie","year":"2008","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1007\/s12038-008-0073-0","article-title":"Emerging and re-emerging bacterial diseases in India","volume":"33","author":"TD Chugh","year":"2008","journal-title":"J Biosci"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"1458","DOI":"10.3201\/eid1109.050493","article-title":"Melioidosis, northeastern Brazil","volume":"11","author":"DB Rolim","year":"2005","journal-title":"Emerg Infect Dis"},{"key":"ref8","first-page":"401","article-title":"Melioidosis in southern India: epidemiological and clinical profile","volume":"41","author":"K Saravu","year":"2010","journal-title":"Southeast Asian J Trop Med Public Health"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1186\/1471-2334-10-302","article-title":"Incidence, risk factors and clinical epidemiology of melioidosis: a complex socio-ecological emerging infectious disease in the Alor Setar region of Kedah, Malaysia","volume":"10","author":"MR Hassan","year":"2010","journal-title":"BMC Infect Dis"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"1538","DOI":"10.3201\/eid0912.020750","article-title":"Intensity of rainfall and severity of melioidosis, Australia","volume":"9","author":"BJ Currie","year":"2003","journal-title":"Emerg Infect Dis"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"970","DOI":"10.1128\/JCM.43.2.970-972.2005","article-title":"Cutaneous melioidosis in a man who was taken as a prisoner of war by the Japanese during World War II","volume":"43","author":"V Ngauy","year":"2005","journal-title":"J Clin Microbiol"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"584","DOI":"10.1128\/IAI.71.1.584-587.2003","article-title":"Biodefense-driven murine model of pneumonic melioidosis","volume":"71","author":"JA Jeddeloh","year":"2003","journal-title":"Infect Immun"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"1761","DOI":"10.1086\/599210","article-title":"Nasal-associated lymphoid tissue and olfactory epithelium as portals of entry for Burkholderia pseudomallei in murine melioidosis","volume":"199","author":"SJ Owen","year":"2009","journal-title":"J Infect Dis"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1146\/annurev.micro.112408.134030","article-title":"Molecular insights into <italic>Burkholderia pseudomallei<\/italic> and <italic>Burkholderia mallei<\/italic> pathogenesis","volume":"64","author":"EE Galyov","year":"2010","journal-title":"Annu Rev Microbiol"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"12095","DOI":"10.1073\/pnas.1107183108","article-title":"Dissection of the <italic>Burkholderia<\/italic> intracellular life cycle using a photothermal nanoblade","volume":"108","author":"CT French","year":"2011","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref16","first-page":"87","article-title":"Virulence determinants in <italic>Burkholderia pseudomallei<\/italic>: Opportunist or accidental pathogen?","volume":"V","author":"IR Beacham","year":"2012"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"5377","DOI":"10.1128\/IAI.68.9.5377-5384.2000","article-title":"<italic>Burkholderia pseudomallei<\/italic> induces cell fusion and actin-associated membrane protrusion: a possible mechanism for cell-to-cell spreading","volume":"68","author":"W Kespichayawattana","year":"2000","journal-title":"Infect Immun"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1111\/j.1365-2958.2004.04528.x","article-title":"Identification of a bacterial factor required for actin-based motility of Burkholderia pseudomallei","volume":"56","author":"MP Stevens","year":"2005","journal-title":"Mol Microbiol"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1111\/j.1462-5822.2006.00807.x","article-title":"The bacterial gene lfpA influences the potent induction of calcitonin receptor and osteoclast-related genes in <italic>Burkholderia pseudomallei<\/italic>-induced TRAP-positive multinucleated giant cells","volume":"9","author":"JA Boddey","year":"2007","journal-title":"Cell Microbiol"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1016\/j.ajpath.2011.03.031","article-title":"Low-dose exposure of C57BL\/6 mice to <italic>Burkholderia pseudomallei<\/italic> mimics chronic human melioidosis","volume":"179","author":"L Conejero","year":"2011","journal-title":"Am J Pathol"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"1901","DOI":"10.1128\/JB.01455-10","article-title":"Identification of motifs of <italic>Burkholderia pseudomallei<\/italic> BimA required for intracellular motility, actin binding, and actin polymerization","volume":"193","author":"C Sitthidet","year":"2011","journal-title":"J Bacteriol"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"1512","DOI":"10.1128\/IAI.01218-10","article-title":"The cluster 1 type VI secretion system is a major virulence determinant in <italic>Burkholderia pseudomallei<\/italic>","volume":"79","author":"MN Burtnick","year":"2011","journal-title":"Infect Immun"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"4839","DOI":"10.1128\/IAI.68.9.4839-4849.2000","article-title":"Bacterial quorum sensing in pathogenic relationships","volume":"68","author":"TR de Kievit","year":"2000","journal-title":"Infect Immun"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1038\/nrm907","article-title":"Listening in on bacteria: acyl-homoserine lactone signalling","volume":"3","author":"C Fuqua","year":"2002","journal-title":"Nat Rev Mol Cell Biol"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"1053","DOI":"10.1099\/jmm.0.45661-0","article-title":"Role of quorum sensing in the pathogenicity of <italic>Burkholderia pseudomallei<\/italic>","volume":"53","author":"RL Ulrich","year":"2004","journal-title":"J Med Microbiol"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"2288","DOI":"10.1128\/JB.186.8.2288-2294.2004","article-title":"The PmlI-PmlR quorum-sensing system in <italic>Burkholderia pseudomallei<\/italic> plays a key role in virulence and modulates production of the MprA protease","volume":"186","author":"E Valade","year":"2004","journal-title":"J Bacteriol"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1128\/JB.187.2.785-790.2005","article-title":"The BpsIR quorum-sensing system of <italic>Burkholderia pseudomallei<\/italic>","volume":"187","author":"Y Song","year":"2005","journal-title":"J Bacteriol"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"3651","DOI":"10.1099\/mic.0.29226-0","article-title":"Quorum sensing regulates <italic>dpsA<\/italic> and the oxidative stress response in <italic>Burkholderia pseudomallei<\/italic>","volume":"152","author":"P Lumjiaktase","year":"2006","journal-title":"Microbiology"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"1176","DOI":"10.1099\/mic.0.046540-0","article-title":"N-Octanoylhomoserine lactone signalling mediated by the BpsI-BpsR quorum sensing system plays a major role in biofilm formation of <italic>Burkholderia pseudomallei<\/italic>","volume":"157","author":"AM Gamage","year":"2011","journal-title":"Microbiology"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"320","DOI":"10.1016\/j.mimet.2008.12.007","article-title":"Facile construction of unmarked deletion mutants in <italic>Burkholderia pseudomallei<\/italic> using sacB counter-selection in sucrose-resistant and sucrose-sensitive isolates","volume":"76","author":"CA Logue","year":"2009","journal-title":"J Microbiol Methods"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"14240","DOI":"10.1073\/pnas.0403302101","article-title":"Genomic plasticity of the causative agent of melioidosis, <italic>Burkholderia pseudomallei<\/italic>","volume":"101","author":"MT Holden","year":"2004","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"993","DOI":"10.1099\/0022-1317-49-11-993","article-title":"Cloning and analysis of genomic differences unique to <italic>Burkholderia pseudomallei<\/italic> by comparison with B. thailandensis","volume":"49","author":"NF Brown","year":"2000","journal-title":"J Med Microbiol"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"773","DOI":"10.1128\/JB.188.2.773-783.2006","article-title":"Specificity of acyl-homoserine lactone synthases examined by mass spectrometry","volume":"188","author":"TA Gould","year":"2006","journal-title":"J Bacteriol"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"4108","DOI":"10.1128\/IAI.65.10.4108-4113.1997","article-title":"Role of reactive nitrogen and oxygen intermediates in gamma interferon-stimulated murine macrophage bactericidal activity against <italic>Burkholderia pseudomallei<\/italic>","volume":"65","author":"K Miyagi","year":"1997","journal-title":"Infect Immun"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"698","DOI":"10.1038\/sj.embor.7400984","article-title":"Quorum sensing has an unexpected role in virulence in the model pathogen <italic>Citrobacter rodentium<\/italic>","volume":"8","author":"SJ Coulthurst","year":"2007","journal-title":"EMBO Rep"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1111\/j.1365-2559.1995.tb00620.x","article-title":"The histopathology of human melioidosis","volume":"26","author":"KT Wong","year":"1995","journal-title":"Histopathology"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"2169","DOI":"10.1128\/EC.00308-07","article-title":"Accidental virulence, cryptic pathogenesis, martians, lost hosts, and the pathogenicity of environmental microbes","volume":"6","author":"A Casadevall","year":"2007","journal-title":"Eukaryot Cell"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1111\/j.1365-2958.2007.05826.x","article-title":"Bacteria between protists and phages: from antipredation strategies to the evolution of pathogenicity","volume":"65","author":"H Brussow","year":"2007","journal-title":"Mol Microbiol"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1111\/j.1462-2920.2007.01238.x","article-title":"Environmental predators as models for bacterial pathogenesis","volume":"9","author":"H Hilbi","year":"2007","journal-title":"Environ Microbiol"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"6865","DOI":"10.1128\/AEM.01036-06","article-title":"Environmental factors that affect the survival and persistence of <italic>Burkholderia pseudomallei<\/italic>","volume":"72","author":"TJ Inglis","year":"2006","journal-title":"Appl Environ Microbiol"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1016\/j.tim.2005.10.004","article-title":"Phage variation: understanding the behaviour of an accidental pathogen","volume":"13","author":"ER Moxon","year":"2005","journal-title":"Trends Microbiol"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1016\/j.tim.2012.04.005","article-title":"Evolution of virulence in opportunistic pathogens: generalism, plasticity, and control","volume":"20","author":"SP Brown","year":"2012","journal-title":"Trends Microbiol"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0063394","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,7,27]],"date-time":"2020-07-27T00:15:46Z","timestamp":1595808946000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0063394"}},"subtitle":[],"editor":[{"given":"Roy Martin Roop","family":"II","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2013,5,21]]},"references-count":42,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2013,5,21]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0063394","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,5,21]]}}}