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Secretaria de Vigil\u00e2ncia em Sa\u00fade. Programa Nacional de DST e AIDS Manaul de Controle das Doen\u00e7as Sexualmente Transmiss\u00edveis-DST. S\u00e9rie Manuais n\u00b0 68. inist\u00e9rio da Sa\u00fade. Bras\u00edlia - DF, 2006. Minist\u00e9rio da Sa\u00fade website. Available at: http:\/\/www.saude.gov.br\/bvs<\/ext-link> Accessed 2013 April 15."},{"key":"ref5","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1186\/1471-2458-12-595","article-title":"Sexually transmitted infections associated syndromes assisted in the primary health care in Northeast, Brazil","volume":"12","author":"EG Cavalcante","year":"2012","journal-title":"BMC Public Health"},{"key":"ref6","unstructured":"Minist\u00e9rio da Sa\u00fade do Brasil. Secretaria de Vigil\u00e2ncia em Sa\u00fade. Programa Nacional de DST e AIDS Preval\u00eancia e freq\u00fc\u00eancias relativas de Doen\u00e7as Sexualmente Transmiss\u00edveis-DST em popula\u00e7\u00f5es selecionadas de seis capitais brasileiras, 2005. Minist\u00e9rio da Sa\u00fade. Bras\u00edlia - DF, 2008. Minist\u00e9rio da Sa\u00fade webiste. Available at: http:\/\/www.saude.gov.br\/bvs<\/ext-link> Accessed 2013 April 15."},{"key":"ref7","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1097\/COH.0b013e32832c1881","article-title":"Herpes simplex virus type 2 and syphilis infections with HIV: an evolving synergy in transmission and prevention","volume":"4","author":"AA Tobian","year":"2009","journal-title":"Curr Opin HIV AIDS"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1093\/infdis\/175.3.583","article-title":"Comparison of clinical diagnosis and standard laboratory and molecular methods for the diagnosis of genital ulcer disease in Lesotho: association with human immunodeficiency virus infection","volume":"175","author":"SA Morse","year":"1997","journal-title":"J Infect Dis"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1097\/00007435-199010000-00007","article-title":"Accuracy of clinical diagnosis of genital ulcer disease","volume":"17","author":"Y Dangor","year":"1990","journal-title":"Sex Transm Dis"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1136\/sti.79.1.68","article-title":"Chancroid: clinical manifestations, diagnosis, and management","volume":"79","author":"DA Lewis","year":"2003","journal-title":"Sex Transm Infect"},{"key":"ref11","doi-asserted-by":"crossref","unstructured":"Gayet-Ageron A, Lautenschlager S, Ninet B, Perneger TV, Combescure C. Sensitivity, specificity and likelihood ratios of PCR in the diagnosis of syphilis: a systematic review and meta-analysis. Sex Transm Infect. 2012 Sep 28. [Epub ahead of print]. doi:10.1136\/sextrans-2012-050622.","DOI":"10.1136\/sextrans-2012-050622"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1007\/978-1-61779-937-2_6","article-title":"The molecular diagnosis of sexually transmitted genital ulcer disease","volume":"903","author":"CY Chen","year":"2012","journal-title":"Methods Mol Biol"},{"key":"ref13","unstructured":"World Health Organization. Department of Reproductive Health and Research Global strategy for the prevention and control of sexually transmitted infections: 2006\u20132015. Breaking the chain of transmission. Geneva, Switzerland: WHO, 2007: 1\u201368. World Health Organization website. Available at: http:\/\/whqlibdoc.who.int\/publications\/2007\/9789241563475_eng.pdf<\/ext-link> Acessed 2013 April 15."},{"key":"ref14","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1128\/JCM.34.1.49-54.1996","article-title":"Simultaneous PCR detection of Haemophilus ducreyi, Treponema pallidum, and herpes simplex virus types 1 and 2 from genital ulcers","volume":"34","author":"KA Orle","year":"1996","journal-title":"J Clin Microbiol"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1128\/JCM.39.2.601-605.2001","article-title":"Diagnosing genital ulcer disease in a clinic for sexually transmitted diseases in Amsterdam, The Netherlands","volume":"39","author":"SM Bruisten","year":"2001","journal-title":"J Clin Microbiol"},{"key":"ref16","unstructured":"Programa Nacional de DST e AIDS Manual de controle de doen\u00e7as sexualmente transmiss\u00edveis. Minist\u00e9rio da Sa\u00fade. Bras\u00edlia, DF, 2006. Minist\u00e9rio da Sa\u00fade website. Available at: http:\/\/www.aids.gov.br\/publicacao\/manual-de-controle-das-doencas-sexualmente-transmissiveis<\/ext-link>. Acessed 2013 April 15."},{"key":"ref17","unstructured":"Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual. In: White BA. PCR Cloning Protocols: From Molecular Cloning to Genetic Engineering (Methods in Molecular Biology). Cold Spring Harbour, New York: Cold Spring Harbor Laboratory Press. p.6.4."},{"key":"ref18","first-page":"807","article-title":"Frequent silencing of the GPC3 gene in ovarian cancer cell lines","volume":"59","author":"H Lin","year":"1999","journal-title":"Cancer Res"},{"key":"ref19","first-page":"249","article-title":"Tzanck smear in diagnosing genital herpes","volume":"64","author":"E Folkers","year":"1988","journal-title":"Genitourin Med"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1097\/00007435-199901000-00009","article-title":"The etiology of genital ulcer disease by multiplex polymerase chain reaction and relationship to HIV infection among patients attending sexually transmitted disease clinics in Pune, India","volume":"26","author":"A Risbud","year":"1999","journal-title":"Sex Transm Dis"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"906","DOI":"10.1097\/OLQ.0b013e3182663e22","article-title":"Genital ulcer disease in India: etiologies and performance of current syndrome guidelines","volume":"39","author":"P Prabhakar","year":"2012","journal-title":"Sex Transm Dis"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1136\/sti.2008.034207","article-title":"Evaluation of multiplex real-time PCR for detection of Haemophilus ducreyi, Treponema pallidum, herpes simplex virus type 1 and 2 in the diagnosis of genital ulcer disease in the Rakai District, Uganda","volume":"85","author":"TR Suntoke","year":"2009","journal-title":"Sex Transm Infect"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1097\/OLQ.0b013e3181bd1007","article-title":"Etiology and determinants of sexually transmitted infections in Karnataka state, south India","volume":"37","author":"M Becker","year":"2010","journal-title":"Sex Transm Dis"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"650","DOI":"10.1258\/ijsa.2009.009067","article-title":"Aetiology of genital ulcer disease in female partners of male participants in a circumcision trial in Uganda","volume":"20","author":"AE Brankin","year":"2009","journal-title":"Int J STD AIDS"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"880","DOI":"10.1097\/OLQ.0b013e318269cf90","article-title":"Prevalence and associations of genital ulcer and urethral pathogens in men presenting with genital ulcer syndrome to primary health care clinics in South Africa","volume":"39","author":"DA Lewis","year":"2012","journal-title":"Sex Transm Dis"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"S97","DOI":"10.1097\/00002030-200108004-00011","article-title":"The epidemiology of HSV-2 infection and its association with HIV infection in four urban African populations","volume":"15","author":"HA Weiss","year":"2001","journal-title":"Aids"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1128\/JCM.38.1.268-273.2000","article-title":"Etiology of genital ulcer disease in Dakar, Senegal, and comparison of PCR and serologic assays for detection of Haemophilus ducreyi","volume":"38","author":"PA Totten","year":"2000","journal-title":"J Clin Microbiol"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"787","DOI":"10.1097\/OLQ.0b013e31826ae97d","article-title":"Etiologic pattern of genital ulcers in Lusaka, Zambia: has chancroid been eliminated?","volume":"39","author":"M Makasa","year":"2012","journal-title":"Sex Transm Dis"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1086\/515603","article-title":"Molecular methods for the diagnosis of genital ulcer disease in a sexually transmitted disease clinic population in northern Thailand: predominance of herpes simplex virus infection","volume":"178","author":"C Beyrer","year":"1998","journal-title":"J Infect Dis"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1136\/sti.79.3.202","article-title":"Increasing relative prevalence of HSV-2 infection among men with genital ulcers from a mining community in South Africa","volume":"79","author":"W Lai","year":"2003","journal-title":"Sex Transm Infect"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"522","DOI":"10.1097\/01.olq.0000137896.40790.7d","article-title":"Changing patterns in sexually transmitted disease syndromes in Kenya after the introduction of a syndromic management program","volume":"31","author":"B Cheluget","year":"2004","journal-title":"Sex Transm Dis"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"1304","DOI":"10.1086\/496979","article-title":"Changes in the etiology of sexually transmitted diseases in Botswana between 1993 and 2002: implications for the clinical management of genital ulcer disease","volume":"41","author":"G Paz-Bailey","year":"2005","journal-title":"Clin Infect Dis"},{"key":"ref33","first-page":"153","article-title":"Etiology of genital ulcer disease. 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