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P, Cameron HD, editors. Online at <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/research.amnh.org\/entomology\/spiders\/catalog\/index.html\" xlink:type=\"simple\">http:\/\/research.amnh.org\/entomology\/spiders\/catalog\/index.html<\/ext-link>: American Museum of Natural History."},{"key":"ref47","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1016\/j.neuron.2004.07.015","article-title":"Tethering naturally occurring peptide toxins for cell-autonomous modulation of ion channels and receptors in vivo","volume":"43","author":"I Ibanez-Tallon","year":"2004","journal-title":"Neuron"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1006\/jmbi.1995.0406","article-title":"Three-dimensional solution structure of the calcium channel antagonist omega-agatoxin IVA: consensus molecular folding of calcium channel blockers","volume":"250","author":"JI Kim","year":"1995","journal-title":"J Mol Biol"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1038\/nature02632","article-title":"A membrane-access mechanism of ion channel inhibition by voltage sensor toxins from spider venom","volume":"430","author":"SY Lee","year":"2004","journal-title":"Nature"},{"key":"ref50","doi-asserted-by":"crossref","first-page":"4379","DOI":"10.1523\/JNEUROSCI.08-11-04379.1988","article-title":"Ionic currents of Drosophila neurons in embryonic cultures","volume":"8","author":"L Byerly","year":"1988","journal-title":"J Neurosci"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"8415","DOI":"10.1073\/pnas.83.21.8415","article-title":"A Drosophila mutation that eliminates a calcium-dependent potassium current","volume":"83","author":"T Elkins","year":"1986","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref52","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1242\/jeb.147.1.21","article-title":"Actions of dendrotoxin on K<sup>+<\/sup> channels and neuromuscular transmission in Drosophila melanogaster, and its effects in synergy with K+ channel-specific drugs and mutations","volume":"147","author":"CF Wu","year":"1989","journal-title":"J Exp Biol"},{"key":"ref53","doi-asserted-by":"crossref","first-page":"2101","DOI":"10.1091\/mbc.8.11.2101","article-title":"E.E. 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