{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T12:45:08Z","timestamp":1791290708122,"version":"4.3.0"},"reference-count":37,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2013,6,10]],"date-time":"2013-06-10T00:00:00Z","timestamp":1370822400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0065114","type":"journal-article","created":{"date-parts":[[2013,6,10]],"date-time":"2013-06-10T17:08:16Z","timestamp":1370884096000},"page":"e65114","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":44,"title":["Circulating Endothelial Cells in Refractory Pulmonary Hypertension in Children: Markers of Treatment Efficacy and Clinical Worsening"],"prefix":"10.1371","volume":"8","author":[{"given":"Marilyne","family":"Levy","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Damien","family":"Bonnet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laetitia","family":"Mauge","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David S.","family":"Celermajer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pascale","family":"Gaussem","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David M.","family":"Smadja","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2013,6,10]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"S43","DOI":"10.1016\/j.jacc.2009.04.012","article-title":"Updated clinical classification of pulmonary hypertension","volume":"54","author":"G Simonneau","year":"2009","journal-title":"J Am Coll Cardiol"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"1219","DOI":"10.1183\/09031936.00139009","article-title":"Guidelines for the diagnosis and treatment of pulmonary hypertension","volume":"34","author":"N Galie","year":"2009","journal-title":"Eur Respir J"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1183\/09059180.00008410","article-title":"Treatment options for paediatric pulmonary arterial hypertension","volume":"19","author":"RM Berger","year":"2010","journal-title":"Eur Respir Rev"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"S74","DOI":"10.1016\/j.rmed.2010.03.020","article-title":"Evidence for the use of combination targeted therapeutic approaches for the management of pulmonary arterial hypertension","volume":"104","author":"DS O'Callaghan","year":"2010","journal-title":"Respir Med"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"2493","DOI":"10.1093\/eurheartj\/ehp297","article-title":"Guidelines for the diagnosis and treatment of pulmonary hypertension: The Task Force for the Diagnosis and Treatment of Pulmonary Hypertension of the European Society of Cardiology (ESC) and the European Respiratory Society (ERS), endorsed by the International Society of Heart and Lung Transplantation (ISHLT)","volume":"30","author":"N Galie","year":"2009","journal-title":"Eur Heart J"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"858","DOI":"10.1183\/09031936.05.00075305","article-title":"Goal-oriented treatment and combination therapy for pulmonary arterial hypertension","volume":"26","author":"MM Hoeper","year":"2005","journal-title":"Eur Respir J"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"584","DOI":"10.1016\/j.jpeds.2010.09.025","article-title":"Add-on therapy with subcutaneous treprostinil for refractory pediatric pulmonary hypertension","volume":"158","author":"M Levy","year":"2011","journal-title":"J Pediatr"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"374","DOI":"10.1161\/CIRCULATIONAHA.108.808246","article-title":"Circulating endothelial cells: a new candidate biomarker of irreversible pulmonary hypertension secondary to congenital heart disease","volume":"119","author":"DM Smadja","year":"2009","journal-title":"Circulation"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"698","DOI":"10.1160\/TH03-04-0251","article-title":"Circulating endothelial cells in pulmonary hypertension","volume":"90","author":"TM Bull","year":"2003","journal-title":"Thromb Haemost"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"1284","DOI":"10.1183\/09031936.00130809","article-title":"Distinct patterns of circulating endothelial cells in pulmonary hypertension","volume":"36","author":"DM Smadja","year":"2010","journal-title":"Eur Respir J"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"869","DOI":"10.1111\/j.1538-7836.2008.02931.x","article-title":"CD146-based immunomagnetic enrichment followed by multiparameter flow cytometry: a new approach to counting circulating endothelial cells","volume":"6","author":"A Widemann","year":"2008","journal-title":"J Thromb Haemost"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1111\/j.1538-7836.2006.01794.x","article-title":"Isolation and enumeration of circulating endothelial cells by immunomagnetic isolation: proposal of a definition and a consensus protocol","volume":"4","author":"A Woywodt","year":"2006","journal-title":"J Thromb Haemost"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"512","DOI":"10.1172\/JCI35070","article-title":"Endothelial-derived FGF2 contributes to the progression of pulmonary hypertension in humans and rodents","volume":"119","author":"M Izikki","year":"2009","journal-title":"J Clin Invest"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/j.vph.2008.06.003","article-title":"Endothelial cell dysfunction and cross talk between endothelium and smooth muscle cells in pulmonary arterial hypertension","volume":"49","author":"M Humbert","year":"2008","journal-title":"Vascul Pharmacol"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.yjmcc.2007.09.006","article-title":"Pathogenic mechanisms of pulmonary arterial hypertension","volume":"44","author":"SY Chan","year":"2008","journal-title":"J Mol Cell Cardiol"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"1655","DOI":"10.1056\/NEJMra035488","article-title":"Pulmonary arterial hypertension","volume":"351","author":"HW Farber","year":"2004","journal-title":"N Engl J Med"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"803","DOI":"10.1016\/j.jacc.2006.09.049","article-title":"Impaired apoptosis of pulmonary endothelial cells is associated with intimal proliferation and irreversibility of pulmonary hypertension in congenital heart disease","volume":"49","author":"M Levy","year":"2007","journal-title":"J Am Coll Cardiol"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"454","DOI":"10.1111\/j.1582-4934.2008.00639.x","article-title":"Circulating endothelial cells, microparticles and progenitors: key players towards the definition of vascular competence","volume":"13","author":"F Sabatier","year":"2009","journal-title":"J Cell Mol Med"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"e10614","DOI":"10.1371\/journal.pone.0010614","article-title":"Reduction of circulating endothelial cells in peripheral blood of ALS patients","volume":"5","year":"2010","journal-title":"PLoS One"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1007\/s10456-012-9306-9","article-title":"Imbalance of circulating endothelial cells and progenitors in idiopathic pulmonary fibrosis","volume":"16","author":"DM Smadja","year":"2013","journal-title":"Angiogenesis"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"927","DOI":"10.1172\/JCI1910","article-title":"Monoclonal endothelial cell proliferation is present in primary but not secondary pulmonary hypertension","volume":"101","author":"SD Lee","year":"1998","journal-title":"J Clin Invest"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"558","DOI":"10.1164\/rccm.200709-1369PP","article-title":"The cancer paradigm of severe pulmonary arterial hypertension","volume":"178","author":"PR Rai","year":"2008","journal-title":"Am J Respir Crit Care Med"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"1270","DOI":"10.1160\/TH05-07-0499","article-title":"Presence of endothelial progenitor cells, distinct from mature endothelial cells, within human CD146+ blood cells","volume":"94","author":"B Delorme","year":"2005","journal-title":"Thromb Haemost"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1007\/s10456-010-9192-y","article-title":"Treprostinil increases the number and angiogenic potential of endothelial progenitor cells in children with pulmonary hypertension","volume":"14","author":"DM Smadja","year":"2011","journal-title":"Angiogenesis"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"1801","DOI":"10.1182\/blood-2006-08-043471","article-title":"Redefining endothelial progenitor cells via clonal analysis and hematopoietic stem\/progenitor cell principals","volume":"109","author":"MC Yoder","year":"2007","journal-title":"Blood"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"2137","DOI":"10.1161\/ATVBAHA.108.168815","article-title":"Bone morphogenetic proteins 2 and 4 are selectively expressed by late outgrowth endothelial progenitor cells and promote neoangiogenesis","volume":"28","author":"DM Smadja","year":"2008","journal-title":"Arterioscler Thromb Vasc Biol"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"2321","DOI":"10.1161\/01.ATV.0000184762.63888.bd","article-title":"PAR-1 activation on human late endothelial progenitor cells enhances angiogenesis in vitro with upregulation of the SDF-1\/CXCR4 system","volume":"25","author":"DM Smadja","year":"2005","journal-title":"Arterioscler Thromb Vasc Biol"},{"key":"ref28","first-page":"733","article-title":"Eisenmenger syndrome a clinical perspective in a new therapeutic era of pulmonary arterial hypertension","volume":"53","author":"M Beghetti","year":"2009","journal-title":"J Am Coll Cardiol"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1160\/TH04-09-0578","article-title":"Circulating endothelial cells. Biomarker of vascular disease","volume":"93","author":"AD Blann","year":"2005","journal-title":"Thromb Haemost"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"13S","DOI":"10.1016\/j.jacc.2004.02.029","article-title":"Cellular and molecular pathobiology of pulmonary arterial hypertension","volume":"43","author":"M Humbert","year":"2004","journal-title":"J Am Coll Cardiol"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"1703","DOI":"10.1016\/S0002-9440(10)64807-9","article-title":"Endothelin-1 induces an angiogenic phenotype in cultured endothelial cells and stimulates neovascularization in vivo","volume":"157","author":"D Salani","year":"2000","journal-title":"Am J Pathol"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1038\/sj.bjp.0706193","article-title":"Endothelin-1 enhances oxidative stress, cell proliferation and reduces apoptosis in human umbilical vein endothelial cells: role of ETB receptor, NADPH oxidase and caveolin-1","volume":"145","author":"F Dong","year":"2005","journal-title":"Br J Pharmacol"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1165\/ajrcmb.25.1.4331","article-title":"Endogenously released endothelin-1 from human pulmonary artery smooth muscle promotes cellular proliferation: relevance to pathogenesis of pulmonary hypertension and vascular remodeling","volume":"25","author":"SJ Wort","year":"2001","journal-title":"Am J Respir Cell Mol Biol"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"440","DOI":"10.1161\/01.CIR.87.2.440","article-title":"Impairment of endothelium-dependent pulmonary artery relaxation in children with congenital heart disease and abnormal pulmonary hemodynamics","volume":"87","author":"DS Celermajer","year":"1993","journal-title":"Circulation"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1183\/09059180.00004510","article-title":"Therapeutic approaches in adults with congenital heart disease-associated pulmonary arterial hypertension","volume":"19","author":"RL Cordina","year":"2010","journal-title":"Eur Respir Rev"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"S410","DOI":"10.1097\/00005344-200036051-00120","article-title":"The prostacyclin-mimetic cicaprost inhibits endogenous endothelin-1 release from human pulmonary artery smooth muscle cells","volume":"36","author":"SJ Wort","year":"2000","journal-title":"J Cardiovasc Pharmacol"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"758","DOI":"10.1161\/ATVBAHA.109.199893","article-title":"Reduced NO-cGMP signaling contributes to vascular inflammation and insulin resistance induced by high-fat feeding","volume":"30","author":"NO Rizzo","year":"2010","journal-title":"Arterioscler Thromb Vasc Biol"}],"updated-by":[{"DOI":"10.1371\/annotation\/ad9cc7fc-af50-4658-bd90-e3aaf0def017","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2014,1,3]],"date-time":"2014-01-03T00:00:00Z","timestamp":1388707200000}}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0065114","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,7,14]],"date-time":"2019-07-14T22:56:10Z","timestamp":1563144970000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0065114"}},"subtitle":[],"editor":[{"given":"James","family":"West","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2013,6,10]]},"references-count":37,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2013,6,10]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0065114","relation":{"correction":[{"id-type":"doi","id":"10.1371\/annotation\/ad9cc7fc-af50-4658-bd90-e3aaf0def017","asserted-by":"object"}]},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,6,10]]}}}