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Available: <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/www.genedb.org\" xlink:type=\"simple\">http:\/\/www.genedb.org<\/ext-link>. Accessed 2013 Apr 10."},{"key":"ref22","doi-asserted-by":"crossref","first-page":"704","DOI":"10.1097\/MPH.0b013e31816916f6","article-title":"Hemophagocytic lymphohistiocytosis associated with Epstein Barr virus and Leishmania donovani coinfection in a child from Cyprus","volume":"30","author":"MG Koliou","year":"2008","journal-title":"J Pediatr Hematol Oncol"},{"key":"ref23","unstructured":"Ouellette M, Ward S (2002) Drug resistance in parasites. In Marr J, Nielsen T, Komuniecki R, editors. Molecular medical Parasitology. New York: Academic Press. 395\u2013430."},{"key":"ref24","first-page":"005","article-title":"In-silico studies of multi drug resistance (MDR) genetic markers of Plasmodium species","volume":"2","author":"CS Yah","year":"2010","journal-title":"J Comput Biol Bioinform Res"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1089\/10766290252913719","article-title":"The Entamoeba histolytica EhPgp5 (MDR-like) protein induces swelling of the trophozoites and alters chloride-dependent currents in Xenopus laevis oocytes","volume":"8","author":"DM Delgadillo","year":"2002","journal-title":"Microb Drug Resist"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"18427","DOI":"10.1016\/S0021-9258(18)55077-8","article-title":"Heavy metal resistance: a new role for P-glycoproteins in Leishmania","volume":"266","author":"HL Callahan","year":"1991","journal-title":"J Biol Chem"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/0166-6851(89)90010-8","article-title":"H DNA amplification in Leishmania resistant to both arsenite and methotrexate","volume":"34","author":"K Katakura","year":"1989","journal-title":"Mol Biochem Parasitol"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"1027","DOI":"10.1002\/j.1460-2075.1990.tb08206.x","article-title":"The amplified H circle of methotrexate-resistant leishmania tarentolae contains a novel P-glycoprotein gene","volume":"9","author":"M Ouellette","year":"1990","journal-title":"Embo J"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"1009","DOI":"10.1002\/j.1460-2075.1991.tb08035.x","article-title":"Direct and inverted DNA repeats associated with P-glycoprotein gene amplification in drug resistant Leishmania","volume":"10","author":"M Ouellette","year":"1991","journal-title":"Embo J"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"2855","DOI":"10.1128\/MCB.12.6.2855","article-title":"Multidrug resistance in Leishmania donovani is conferred by amplification of a gene homologous to the mammalian mdr1 gene","volume":"12","author":"DM Henderson","year":"1992","journal-title":"Mol Cell Biol"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/0166-6851(93)90028-V","article-title":"Molecular characterization of the ldmdr1 multidrug resistance gene from Leishmania donovani","volume":"60","author":"N Hendrickson","year":"1993","journal-title":"Mol Biochem Parasitol"},{"key":"ref32","unstructured":"Blast website. 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